cyclic nucleotide regulation of globin genes in erythropoiesis

... acquisition of microRNA-mediated regulation Considering the pervasive nature of microRNA regulation in mammalian cells, it is intriguing to inquire how the function and evolution of duplicate genes ... 3'UTRs than singleton genes (p < × 105 for PicTar targets) However, upon examining the density of target sites in the 3'UTR, defined as the number of distinct microRNA target binding sites types ... singleton genes are under the same level of selective pressure, we normalized rates of substitution for the 3'UTR of each gene by the corresponding rate of substitution of the coding region in...

Ngày tải lên: 14/08/2014, 20:22

... oligonucleotide dimer (Fig 3B, columns and 3) Translational regulation of iPLA2c in myocardium Owing to the obvious complexity of the regulation of iPLA2c resulting from the combined presence of ... presence of multiple iPLA2c protein products in mitochondria resulting from usage of different translation initiation codons in rat myocardium Alternative splicing of iPLA2c in mammalian tissues In ... 271) rate-determining factor in uncoupling protein (UCP) activity [16] Thus, the role of mitochondrial iPLA2 activities in regulating mitochondrial function is just now beginning to be understood...

Ngày tải lên: 19/02/2014, 16:20

... Expression of prion genes in the developing zebrafish Fig Alignment of the conserved sequence motifs of the C-terminal domain among members of the PrP family Amino acid numbering starts from the initiator ... be of interest because in terms of function, the fish intestine is highly regionalized both in the larva and in the adult [38] The posterior segment of the fish intestine is the absorption site of ... routing of PrPSc in the early steps of infection Our observations of a prp2 hybridization signal in the posterior intestine of zebrafish larvae call for evaluation of the potential prions uptake of...

Ngày tải lên: 19/02/2014, 16:20

... construction of a versatile system for the expression of foreign genes in plants Gene 61, 1–11 Tavva VS, Collins GB & Dinkins RD (2006) Targeted overexpression of the Escherichia coli MinC protein in higher ... a DNAbinding domain (DBD) and a ligand-binding domain (LBD) The chimeric gene (transactivation domain– DBD–LBD) is expressed under the control of a constitutive promoter In the presence of a specific ... flexible ligand binding pocket of ecdysone receptor: a single amino acid change leads to discrimination between two groups of nonsteroidal ecdysone agonists J Biol Chem 279, 27211–27218 Dinkins RD, Pflipsen...

Ngày tải lên: 16/03/2014, 06:20

... observed by Kummel in 1988 [57], is explained by an increased turnover of ADP and ATP inside the ICEUs owing to the channelling of both nucleotides Indeed, if the accumulation of ADP inside the ICEUs ... turnover of contractile cycles during high workloads and as a result of the presence of creatine kinase and adenylate kinase systems, the channelling of adenine nucleotides and metabolic signalling ... creatine kinase reactions, including mitochondrial creatine kinase [3840] Increase of the free calcium concentration to lm resulted in some decrease of the Vmax, but its value stayed higher in...

Ngày tải lên: 16/03/2014, 22:20

... role of the N-terminal part of the kinase, containing a PAS domain, was established in signal transduction between the RH and the kinase [6,7] Addition of H2 to HupUV before or during the incubation ... Comparison of HupSL regulations and the functional roles of HupTUV in other T roseopersicina strains would provide further insight into the understanding of the loss of HupSL hydrogenase regulation ... hydrogenase genes [25] In Rhodopseudomonas palustris CGA009, the photosystem is synthesized in the dark due to a single point mutation in the helix–turn–helix DNA binding motif of PpsR, rendering it inactive...

Ngày tải lên: 16/03/2014, 23:20

... the increased rate of 13C label incorporation into the C6 position of glycogen in the ADM group is indicative of activated 6-phosphofructo-1-kinase, which can lead to increased 13C labeling at ... start of glucose infusion For the calculation of glycogen C6 changes, data acquired within h of the start of the glucose infusion was not included to avoid any in uence of transient changes in the ... up -regulation of glucokinase and a down -regulation of glucose-6-phosphatase gene expression, suggesting that this biofactor may also be involved in balancing the uptake and release of glucose from...

Ngày tải lên: 17/03/2014, 17:20

... points following infection with varying MOIs Analysis of recombinant protein production in these samples will indicate how much virus is needed to obtain maximal yield after a given time of infection ... as neomycin or hygromycin, positive transfectants can be selected in media containing the corresponding antibiotic Induction of gene amplification using glutamine synthetase22 or dihydrofolate ... amount of protein needed in combination with the intended use (in vitro, in vivo, structural biology) The design of a suitable expression vector for an expression system of choice, also including...

Ngày tải lên: 11/04/2014, 09:41

... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA ... Switzerland 2Department of Immunology, Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) ... (group A) is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15,18] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include...

Ngày tải lên: 18/06/2014, 19:20

... studying the influence of SARS- CoV on the immune system-independent induction of cytokines Interferon genes and their antiviral effectors To properly assess the cytokine profile of SARS-CoV infection, ... production of chemokines by the infected tissue cells Results Virus-induced cytokines not only play a significant role in host defense, but also in immunopathogenesis Investigations of the cytokine profiles ... dissemination of SARS-CoV By slowing down expression of IFNs and their antiviral genes in the infected tissue cells, the virus buys time during the initial, critical phase of infection in order...

Ngày tải lên: 20/06/2014, 01:20

... biology, in view of proteomic experiments demonstrating that a single miRNA can directly repress the production of hundreds of proteins in mammalian cells, through both downregulation of mRNA ... Switzerland 2Department of Immunology, Page of National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan Laboratory of Experimental Hematology, Bordet Institute, University of Brussels (ULB) ... (group A) is in agreement with previous cloning studies in mouse CD8+ lymphocytes [15,18] showing that a few highly expressed microRNAs prevailed in frequency Interestingly, these microRNAs include...

Ngày tải lên: 20/06/2014, 03:20

... in turn comprises original data from gene- and genomesequencing projects An independent database is maintained by the RIKEN Institute in Japan and contains the sequences of over 60,000 full-insert ... the general problem of learning to discriminate between positive and negative members of a given class of n-dimensional vectors The SVM works by mapping a given training set into a multi-dimensional ... advance in helping to unravel the functions of the tens of thousands of human genes using functional genomics approaches.” Journal of Biology 2004, Hughes has enjoyed the transition from studying...

Ngày tải lên: 06/08/2014, 18:21

... abrupt changes in the levels of some yeast transcripts, with increases in expression of key genes involved in glucose transport and vesicle transport and repression of those involved in ethanol metabolism ... decrease in levels of glutamate Instead, there was a corresponding trend in the increase in the transcript and protein levels of the mitochondrial 2-oxoglutarate transporters, and an increase in protein ... gain insights into the importance of mitochondrial energy and nitrogen metabolism in affecting growth rates These insights were possible only by integrating information from transcripts, protein...

Ngày tải lên: 06/08/2014, 18:21

... the sequence of CaMV35S promoter which located infront of AtAOS2 gene in pCAMBIA1201/AtAOS binary vector (Fig 3) PCR was carried out according to the PCR Amplification Kit by using HiPi PCR Premix ... level of endogenous JA in rice, we try to over express AOS genes by transforming AtAOS2 gene to the rice genome Fig The octadecanoid pathway leading to jasmonic acid (JA) biosynthesis in plants ... Dr.Eunsun Kim) In this study, a set of PCRs was carried out to detect the hygromycin resistance gene, CaMV35S promoter (the promoter located in front of the AtAOS2 gene), and the AtAOS2 gene in the transgenic...

Ngày tải lên: 06/08/2014, 18:21

... layer protein) Fibronectin PRELP (proline/arginine-rich and leucine-rich repeat protein) Chondroadherin Fibrillin Tenascin-C Elastin MGP (matrix Gla protein) Chondromodulin-I Chondromodulin-II CD-RAP ... [23], linkage analysis of 14 candidate genes in OA kindreds resulted in the exclusion of 10 important cartilage genes, including COL2A1 [40] Analysis of 47 families with the phenotype of earlyonset ... thrombospondin family of matrix molecules and which is localized in the pericellular, territorial matrix of chondrocytes The protein contains several repeat domains, including eight calcium-binding,...

Ngày tải lên: 09/08/2014, 06:22

... complexes in yeast: mining large scale protein-protein interaction screens Bioinformatics 2003, 19:1901-1908 Manke T, Bringas R, Vingron M: Correlating protein-DNA and protein-protein interaction ... determined the overlap between known sets of co-regulated genes in yeast and groups of genes coding for components of individual multiprotein complexes A set of co-regulated genes is defined here ... 'core' of the binding site, and the number in brackets is the length of the spacer in terms of the number of intervening nucleotides The feature map shows that each upstream sequence contains at...

Ngày tải lên: 09/08/2014, 20:20

... Higher H3K9ac on inducible genes is independent of the presence of CpG islands Higher H3K9ac on inducible genes is independent of the presence of CpG islands (a) The percentage of genes with CpG ... group of genes and to examine the H3K9ac levels for a set of well characterized inducible genes at the promoter region (e) In (d) the genes are plotted from the left to right in order of decreasing ... chromatin signature of inducible genes in T cells To facilitate comparison of genes with similar basal expression levels, genes were binned according to their basal expression levels determined...

Ngày tải lên: 09/08/2014, 20:20

... multiple lines of evidence suggesting the potential functional importance of the fusion genes, including the involvement of known oncogenic partner genes, exclusive expression of the partner genes ... phenotype In order to gain insight into the functional role of the novel fusion genes, we performed small interfering RNA (siRNA) knock-down analysis targeting the parts of the 3’ partner genes that ... reported to interact with Bcl-xL, and Ras in T-cells, resulting in the inhibition of apoptosis [29,30] IKZF3 is located at the most common telomeric breakpoint of the ERBB2amplicon [31] Interestingly,...

Ngày tải lên: 09/08/2014, 22:23

... The use of fenofibrate was related to significant decreases in the expression in circulating leukocytes of CX3CL1 and CCR2 genes and a significant increase in the expression of SDF-1 Page of (page ... documented role in the local accumulation of monocytes [8,9] However, fenofibrate had an intriguing effect in SDF-1 expression neutralizing the significant decrease in its expression observed in untreated ... significant effect of fenofibrate in the MCP-1/CCL2 gene expression However, there was a significant decrease in the gene expression of the CX3CL1/ Fractalkine in patients treated with fenofibrate with...

Ngày tải lên: 10/08/2014, 05:21

... strength of microarray interpretation lies in the possibility of extracting information beyond the single gene level to address questions on the co -regulation of genes, on the identification of gene ... successfully applied for data mining The main strength of GeneTrail was that it offered answers to individual biological questions with its feature of incorporation of individually defined categories (such ... changes in external Fe supply or in internal regulators of metal chelation and transport resulted in significant alterations of gene expression patterns of an entire category of genes representing...

Ngày tải lên: 11/08/2014, 11:20