Inactivation and repair of escherichia coli following UV disinfection influencing factors and photolyase activity

... Background 88 4.2 UV Inactivation of E coli 90 4.3 Photoreactivation of E coli following UV Disinfection 94 4.4 Dark Repair of E coli following UV Disinfection 100 4.5 Comparison of Repair of Selected ... Photoreactivation of microorganisms after UV disinfection 50 2.9.4 Factors affecting photoreactivation 53 2.9.5 Dark repair 58 UV Disinfectio...

Ngày tải lên: 11/09/2015, 16:03

... apparent photoreactivation under sunlight following UV inactivation VRE exhibited apparent photoreactivation The dose of UV light required for 90% inactivation of VRE with and without photoreactivation ... Figure (Tosa and Hirata, 1999) VRE was the most UV resistant bacteria tested, while P aeruginosa and EHEC were weaker The UV doses required for 90% inactiv...

Ngày tải lên: 05/09/2013, 08:40

... evaluate the effects of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and ... Effects of ionic strength and divalent cations on the activity and specificity of Fpg and OGG1 The conditions inside a living cell differ from...

Ngày tải lên: 18/02/2014, 18:20

... with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ in the ... that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD...

Ngày tải lên: 21/02/2014, 01:21

... the C-terminal domain, the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in expression of the maximum catalytic activity, and in allosteric regulation ... Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta...

Ngày tải lên: 06/03/2014, 11:20

... 1996) In conclusion, our data demonstrate alarmingly high individual and multiple resistance to antibiotics in E coli, reflecting the misuse of these agents in the poultry industry Since chicken ... Clinical Laboratory Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isola...

Ngày tải lên: 08/03/2014, 09:20

... mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated ... LPS (R4 core-type) is as depicted in Fig Structural analysis of E coli F653 core-oligosaccharides (R3 core) We have subjected purified LPS from E c...

Ngày tải lên: 08/03/2014, 09:20

... with the amino group and the N3 atom of the cytosine (Fig 1) This study uses site-directed mutagenesis to further explore the contribution of these amino acids interacting with the pyrimidine ring ... consisting of three domains, the CORE, the LID and the NMPbind [1] A characteristic of bacterial CMP kinases is an extension of the NMPbind domain by 40...

Ngày tải lên: 16/03/2014, 10:20

... hypothesis The demonstration of the IIACrr MalK interaction suggests that IIACrr may regulate the function of some of the > 140 MalK homologues found in the S coelicolor genome The one with the ... investigate is whether the mechanism of inducer exclusion is realized in S coelicolor Our observation that IIACrr could replace the inducer exclusion...

Ngày tải lên: 17/03/2014, 23:20

... intensity of the GlnB band was quantied as the sum of the concentrations of GlnB and GlnK and was denoted by PII* Note that PII* includes the modied forms of GlnB and GlnK as well, i.e GlnBUMP and ... bars indicate the SEM The dotted line is a result of two linear regression ts of the data points The extra abscissa on top of the gure indicates...

Ngày tải lên: 23/03/2014, 04:21

... small compared with those of G270V, E406G, and R166G Changes at the surface of the protein not appear to be major contributors to the solubility of the AMPP fragments The AMPP protein appears to have ... recombinant E coli AMPP and its domain variants Creating a library for truncated AMPP fragments The 1.3 kb pepP gene encoding E coli AMPP was PCR amp...

Ngày tải lên: 23/03/2014, 07:20

... and D), and therefore HOQNO binding cannot be detected by its perturbation of the EPR spectrum of the oxidized enzyme (see below) In contrast to the results of Hagerhall et al [20], the ¨ ¨ EPR ... understanding of the effects of the mutants on the physiological quinol oxidation reaction catalyzed by FrdABCD, we studied their effects on the steady-state k...

Ngày tải lên: 23/03/2014, 13:20

... residues reacted with DTNB (Table 1) The residues 4261 Disulfides of apoFNR Fig MALDI-TOF spectra of aerobically (A) and anaerobically (B) prepared and carboxymethylated apoFNR The samples of apoFNR ... Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ and ‘anaerobic’ apoFNR, and apoFNR obtained by air-induced inactiva...

Ngày tải lên: 23/03/2014, 15:20

... inactivation of wild-type CTPS by trypsin (A) Inactivation of CTPS-catalysed NH3-dependent CTP formation in the absence of ligands (s) and in the presence of ATP (10 mM, n), UTP (10 mM, h), and ... mutant CTP synthases The values of apparent kcat for the hydrolysis of glutamine by K187A (d) and R429A (s) apparent CTP synthases are shown Inset: values of kcat...

Ngày tải lên: 23/03/2014, 17:22

... anchoring by E coli PBPs 4, 5, and 6b (Eur J Biochem 269 ) 5827 Fig Helical wheel representations of protein segments The C-terminal sequences of PBP4, PBP5, PBP6 and PBP6b, and the primary sequence of ... 0 .65 0.23 Ó FEBS 2002 Membrane anchoring by E coli PBPs 4, 5, and 6b (Eur J Biochem 269 ) 5823 The peptides P4 and P5 (Table 1) were supplied...

Ngày tải lên: 31/03/2014, 08:20