co transduction and location of escherichia coli genetic markers

... of Verocytotoxinproducing Escherichia coli O157 on Dutch dairy farms J Clin Microbiol 1998, 36, 3480-3487 22 Hitchins AD, Feng P, Watkins WD, Rippey SR, Chandler LA Escherichia coli and the Coliform ... investigation of E coli O157:H7 isolates and reference strains Based on the results obtained, primer CRA22 and CRA23 were commercially synthesized for analysis of E coli O157:H7 strains Twenty ng of each ... immunomagnetic particles for the separation of E coli O26 and O111 have become commercially available With the use of IMS, the isolation rate of E coli O157 has been markedly improved Wright...

Ngày tải lên: 07/08/2014, 18:21

... Percentage log recovery of E coli ATCC 11229 and ATCC 15597 following h of fluorescent light and sunlight exposure with LP and MP UV disinfection 126 Table 5-2 Comparison of repair rates of E coli ATCC ... 2.9.5 Dark repair 58 UV Disinfection and DNA Repair of Escherichia coli 59 2.10.1 UV disinfection of E coli 59 2.10.2 DNA repair of E coli 61 CHAPTER MATERIALS AND METHODS 66 3.1 Overview 66 3.2 ... values of E coli ATCC 11229 and E coli ATCC 15597 after LP and MP UV disinfection, followed by h of incubation at various temperatures and constant light intensity of 11.5 kLux 131 Figure 5-6 Concentrations...

Ngày tải lên: 11/09/2015, 16:03

... Sequence of recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI-II (ReMCoTI-II) The expression conditions such as ampicillin and IPTG concentrations, temperature and ... Chitin column chromatography Washing column, on column cleavage (40C/40hours) ReMCoTI-II Freez dryer ReMCoTI-II powder Fig 11: Purification scheme of recombinant MCoTI-II using chitin beads column ... recombinant MCoTI-II were followed the manufactures instruction of IMPACT-CN System, using chitin beads column chromatography [7] Results 3.1 Design and construction of the gene coding for MCoTI-II...

Ngày tải lên: 12/02/2014, 10:20

... of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and ... characterization of the Fpg family of DNA glycosylases DNA Repair 2, 839–862 Boiteux S, O’Connor TR & Laval J (1987) Formamidopyrimidine-DNA glycosylase of Escherichia coli: cloning and sequencing of the ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1...

Ngày tải lên: 18/02/2014, 18:20

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... products of agmatinase with the GfiA and CfiT substitutions in the coding and noncoding strands, respectively, and using the 5¢ and 3¢ primers mentioned above, the full length agmatinase cDNA coding ... (1.6 ± 0.1 mM) and the Ki for competitive Fig Effect of added Mn2+ and EDTA on the catalytic activity of wild-type and D153N agmatinase The enzymes were assayed before (Control) and after dialysis...

Ngày tải lên: 21/02/2014, 01:21

... manufacturer’s recommendations Protein expression and purification E coli Pta and Pta-F1, Pta-F2 and Pta-F3 were produced in E coli K-12 AG1, E coli BL21(DE3) or E coli M15 containing the corresponding ... pCA24N–Pta; E coli BL21(DE3) for pET28–F1 and pET28–F2; and E coli M15 for pQE30–F3 Construction of the E coli acs pta deletion strain The E coli acs pta deletion strain (FB22) was constructed ... al Escherichia coli phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli...

Ngày tải lên: 06/03/2014, 11:20

... Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken isolates E coli- patient ... Characterization of Escherichia coli isolated from cases of avian colibacillosis Canadian Journal of Veterinary Research 57, 146–151 Allen DG, Pringle X, Smith D, Conlon PD & Burgmann PM (1993) Handbook of ... isolates Serotyping of 119 and 100 E coli organisms isolated from chicken and patients, respectively, is presented in Table Only 30.3% of chicken E coli isolates and 38% of patient isolates were...

Ngày tải lên: 08/03/2014, 09:20

... based conformational analysis of the inner core region of enterobacterial LPS and epitope mapping of WN1 222-5 we have in addition isolated the complete core-oligosaccharides of E coli F653 (R3-core) ... of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the latter ... main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated minor core-oligosaccharide of E coli F653 (R3 core-type) was analyzed...

Ngày tải lên: 08/03/2014, 09:20

... Overproduction and molecular characterization of the modied variants of E coli CMP kinase The wild-type and various modied forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli CMP kinase ... eukaryotic and bacterial NMP kinases concerns the recognition of pyrimidine nucleotides The structure of E coli CMP kinase in complex with CMP or dCMP showed that discrimination between CMP and UMP ... had no consequence on binding or phosphorylation of the corresponding NMPs (A37 and T39 in these enzymes are equivalent to S36 in E coli CMP kinase) Substitution of R110 with Met in E coli CMP...

Ngày tải lên: 16/03/2014, 10:20

... assayed by complementation of the glucose-specific PTS of E coli measuring phosphoenolpyruvate-dependent phosphorylation of methyl [a-14C]glucoside ([14C]aMG; Amersham) in the presence of E coli LM1 ... encodes ribosomal RNA and downstream by an ORF of unknown function The sequence of the crr region contains two possible start codons Analysis of the Fig Genetic organization of the S coelicolor ... of glucose was restored as indicated by red glucose-fermenting colonies on MacConkey agar supplemented with glucose (Fig 5) This showed that IIACrr could interact with the E coli components of...

Ngày tải lên: 17/03/2014, 23:20

... utilization in Escherichia coli J Bacteriol 150, 214220 72 Bueno R, Pahel G & Magasanik B (1985) Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli J Bacteriol ... absence of ammonia: GlnB versus GlnK Above we measured the primary effect of modulating the expression of glnB in terms of the concentration of PII*, which corresponds to the sum concentration of ... Scanning of the autoradiograms and the determination of intensity of the PII* and PII*UMP bands were as described above Some gels did not result in a complete separation of the uridylylated and native...

Ngày tải lên: 23/03/2014, 04:21

... Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase P insights into substrate recognition and the mechanism of catalysis ... well as its C-terminal domain of E coli aminopeptidase P sequence The location of the domain boundary was estimated by inspection of the structure, and this was compared with fragment lengths ... digestion of the pepP gene A set of nested truncated pepP genes was fused to that of DHFR in the fusion vector pJWL1030folA and transformed into competent E coli cells Two libraries of about 10...

Ngày tải lên: 23/03/2014, 07:20

... electron-transferring cofactor, it could provide a conduit for electron-transfer from the QD site to the QP site However, analyses of the bioenergetics of respiratory growth of E coli on fumarate ... binding to E coli fumarate reductase Table Effect of the FrdABCD mutations on HOQNO binding determined by FQ titrations and EPR spectroscopy in E coli strain DW35 The concentration of the dithionite-reduced ... spin-coupled pair of [4Fe-4S] clusters and comprises a peak at g ¼ 2.29, and troughs at g ¼ 1.87 and 1.67 Addition of HOQNO causes the appearance of a peak at g ¼ 1.98 (compare Figure 4Ci and ii) Overall,...

Ngày tải lên: 23/03/2014, 13:20

... of the cysteinyl residues in apoFNR and in reconstituted [4Fe-4S]ÆFNR ApoFNR that was devoid of Fe and acid-labile sulfur (not shown) was obtained by isolation of FNR from E coli under oxic and ... Therefore the redox state of the cysteinyl residues of the various forms of apoFNR – ‘aerobic’ and ‘anaerobic’ apoFNR, and apoFNR obtained by air-induced inactivation of [4Fe-4S]ÆFNR – was determined ... fivefold alkylated apoFNR, and a minor signal of threefold alkylated FNR (Fig 1B) Therefore, aerobic and anaerobic apoFNR are mixtures of FNR with one, three and five, and five and three cysteine...

Ngày tải lên: 23/03/2014, 15:20

... Sequence alignments were conducted using CLUSTALW [36] Enzyme expression and purication Wild-type and mutant forms of recombinant E coli CTPS were expressed in and puried from E coli strain BL21(DE3) ... SDS/PAGE analysis of trypsin-catalysed cleavage of recombinant wild-type CTPS in the absence and presence of ligands For each gel: lane contains molecular mass standards and lanes 26 contain wild-type ... formation of tetramers [53] Predictions of the secondary structure of the highly conserved region of amino acid sequence between residues 185 and 192 suggest that Lys187 constitutes part of a conserved...

Ngày tải lên: 23/03/2014, 17:22

... mechanisms of membrane anchoring of Escherichia coli penicillin binding proteins FEMS Microbiol Rev 13, 1–12 Ehlert, K & Holtje, J.-V (1996) Role of precursor translocation in coordination of murein and ... subtraction of baselines resulting from neighboring bands Similar to the deconvolution technique, the bandshapes of the single components are superpositions of Gaussian and Lorentzian bandshapes ... temperature (Figs and 6) Control experiments recorded the Tc of both Myr2-PGro and Myr2PCho membranes as 25 °C and that of Myr2-PEtn membranes as 47 °C (Figs 3A–C and 6A)C) In the presence of P4, no...

Ngày tải lên: 31/03/2014, 08:20

... Microarray: A New Tool for Typing Genetic Variants of the LEE Pathogenicity Island of Human and Animal Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic E coli (EPEC) Strains Clin Chem ... Isolation of recombinant antibodies against EspA and intimin of Escherichia coli O157:H7 J Clin Microbiol 2004, 42(7):2966-76 Garrido P, Blanco M, Moreno-Paz M, Briones C, Dahbi G, Blanco J, Blanco ... basis of the common sequence found in different strains of E coli O157 H7 Figure A: Codon usage analysis of wild type and optimized gene for expression in plants A: Codon usage analysis of wild...

Ngày tải lên: 13/08/2014, 16:20

... classes Pattern codes are represented as 6-tuples of ones and twos (for allele and allele 2) in the following order: (K) E coli K-12 MG1655, (O) E coli O157:H7 EDL933, (O) E coli O157:H7 Sakai ... intraspecies recombination, which appear to be quite common, and rates of recombination of orthologous genes between species such as E coli and Salmonella, which appear to be much less frequent [2] Conclusion ... reports 11 Milkman R: Recombination and population structure in Escherichia coli Genetics 1997, 146:745-750 Daubin V, Moran NA, Ochman H: Phylogenetics and the cohesion of bacterial genomes Science...

Ngày tải lên: 14/08/2014, 16:21

... States [2] and probably evolved from a progenitor of E coli O55:H7, a source of infantile diarrhea [3] The STEC O157 5.5-Mb genome contains a 4.1-Mb backbone that is shared with E coli K-12 and thought ... numbers correspond with genotype sequences recorded in Additional data file and the units are color coded by genotype for the tir 255 T>A polymorphism and host origin Comparison of PFGE and polymorphism-derived ... sequence of enterohaemorrhagic Escherichia coli O157:H7 Nature 2001, 409:529-533 Feng P, Lampel KA, Karch H, Whittam TS: Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7...

Ngày tải lên: 14/08/2014, 21:20

... Biochemical and structural studies of Escherichia coli Lrp Transcriptional regulation of gene expression is an essential component for the life of any organism For uropathogenic Escherichia coli, expression ... Purification and Characterization of Lrp and PapI 2.1 Purification and characterization of Lrp 2.1.1 Materials and Methods Purification of E coli Leucine-resposive regulatory protein E coli Lrp was ... hand corner of the graph and proceeds to the right The unmelting curve begins at the top right hand corner of the graph and proceeds to the left 30 Purification and Characterization of Lrp and...

Ngày tải lên: 14/11/2014, 06:09