rnase ii of escherichia coli

... specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2 (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of cleavage of 8-oxoG:C ... specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of ... specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the absence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of...

Ngày tải lên: 18/02/2014, 18:20

... native form of Hfq has an apparent molecular mass of 50 ± 10 kDa, while the unfolded state of Hfq has an average apparent molecular mass of 12 ± kDa This indicates that the unfolded state of Hfq was ... binding of the polyadenylated rpsO RNA We have shown that the presence of the remainder of the acidic tail results in the thermodynamic stabilization of Hfq by 1.8 kcalÆmol)1 A comparison of the ... destabilize the network of hydrogen bonds The crystal structure of the first 71 amino acids of E coli Hfq indicates that the C-terminal domain is probably located at the top of the ring, because...

Ngày tải lên: 19/02/2014, 12:20

... 0.3-nM for DNA duplexes I, II and III, respectively Thus, the binding efficiency of the reactive DNA duplexes I and II was similar to the binding efficiency of DNA duplex III, which has the same sequence ... into the oxoG-containing strand of a 29/22-mer DNA duplex 5¢ to oxoG (duplex I) or 5¢ to the oxoG-pairing cytidine residue of the other strand (duplex II) DNA duplex III did not contain any dU* residue ... the cleavage reaction with DNA duplexes I–III DNA duplexes I and II are incised by the enzyme The efficiency of DNA incision was dependent on the position of the TFMDPh group and was higher for DNA...

Ngày tải lên: 20/02/2014, 11:20

... Fraenkel, D.G (1972) Suppressor of phosphofructokinase mutations of Escherichia coli J Bacteriol 112, 183–187 29 LeMaster, D & Kushlan, D (1996) Dynamical mapping of E coli thioredoxin via 13C NMR ... expression of the fumarase gene of Escherichia coli K-12 J Bacteriol 153, 588–596 31 Model, P & Rittenberg, D (1967) Measurement of the activity of the hexose monophosphate pathway of glucose ... key aspects of functionality and regulation Nature 420, 190–193 44 Bachmann, B.J (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 In Escherichia coli and Salmonella:...

Ngày tải lên: 20/02/2014, 23:20

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... superimposition of the structures of B caldovelox arginase (red), rat liver arginase (yellow) and the modelled structure of E coli agmatinase (blue) Note the shorter extension, in the case of E coli agmatinase, ... properties of agmatine ureohydrolyase, a putrescine biosynthetic enzyme in Escherichia coli J Bacteriol 165, 843–848 Buch, J.K & Boyle, S.M (1985) Biosynthetic arginine decarboxylase in Escherichia coli...

Ngày tải lên: 21/02/2014, 01:21

... with the C-terminal domain of PtaIIs A B Pi Acetyl-CoA Thus, the active site of PtaIIs is probably located at the C-terminal end of the protein, and the role of the PtaII N-terminal domain has ... al Escherichia coli phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli ... Activation of the acetyl phosphate synthesis activity of E coli Pta by different concentrations of pyruvate and PEP Results are presented as percentage of activity in the presence of PEP or pyruvate...

Ngày tải lên: 06/03/2014, 11:20

... from Escherichia coli: purification and properties J Bacteriol 163, 1–7 10 Cai K, Schirch D & Schirch V (1995) The affinity of pyridoxal 5¢-phosphate for folding intermediates of Escherichia coli ... shape of the dimeric protein Depletion of the cofactor affected the quaternary structure of both single mutants, which showed an extra sedimentation peak, at approximately 2.7S in the case of L85A ... comparison of the dissociation constants of the single mutant apoforms, obtained by a fitting of the sedimentation equilibrium curves to a monomer-dimer model, indicates that the destabilizing effect of...

Ngày tải lên: 07/03/2014, 03:20

... 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken isolates E coli- patient isolates ... Characterization of Escherichia coli isolated from cases of avian colibacillosis Canadian Journal of Veterinary Research 57, 146–151 Allen DG, Pringle X, Smith D, Conlon PD & Burgmann PM (1993) Handbook of ... pathogens: Escherichia coli 0157: H7 as a model of entry of a new pathogen into the food supply of the developed world Epidemiological Review 18, 29–51 Aronson AL (1975) Potential impact of the use of...

Ngày tải lên: 08/03/2014, 09:20

... accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12 J Mol Biol 189, 449–455 34 Lugtenberg, B., Peters, R., Bernheimer, H & Berendsen, W (1976) Influence of cultural ... composition of the outer membrane proteins of Escherichia coli Mol Gen Genet 147, 251–262 35 Bosch, D., de Boer, P., Bitter, W & Tommassen, J (1989) The role of the positively charged N-terminus of the ... of proteins across membranes: the helix reversion hypothesis Biosci Rep 9, 737–745 Iino, T & Sako, T (1988) Inhibition and resumption of processing of the staphylokinase in some Escherichia coli...

Ngày tải lên: 08/03/2014, 09:20

... structure of E coli R3 and R4 LPS (Eur J Biochem 269) 5983 Lipopolysaccharide (LPS) is the major component of the outer leaflet of the outer membrane of Gram-negative bacteria [1] LPS of enterobacteria ... core-oligosaccharides of the R1 and R2 core-types [8] whereas the chemical structure of the inner core-region of the E coli R4 core-type was hitherto unknown The chemical structure of the outer core region of the ... HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated minor core-oligosaccharide of E coli F653...

Ngày tải lên: 08/03/2014, 09:20

... of India, and MHRD, Government of India, for financial support We also thank Professor Winfried Boos, University of Konstanz, Germany, for the gift of plasmids encoding His-tagged MalZ and Professor ... protection of MalZ activity in vitro [dithiothreitol] (mM) Fig Determination of the optimum concentrations of dithiothreitol and MgCl2 for the in vitro refolding of MalZ (A) The denatured solution of ... activity of the protein was monitored The control refolding experiment of MalZ was carried out in absence of any osmolyte It was observed Table The effect of different concentrations (M) of chemical...

Ngày tải lên: 16/03/2014, 06:20

... from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein ... of microgram quantities of protein utilizing the principle of proteindye binding Anal Biochem 72, 248254 30 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of ... top of sequences indicate conserved residues involved in the interaction with the phosphate moieties of various NMPs (R41, R131 and R181 in E coli CMP kinase) and the four modied residues of E coli...

Ngày tải lên: 16/03/2014, 10:20

... (D) Conformer I of the crystal structure dimer of E coli DnaG-C [4] (E) Conformer II of the crystal structure dimer of E coli DnaG-C [4] structure of DnaG-C as a domain-swapped dimer, where helix ... points in helix of E coli DnaG-C observed in the crystal structure (Fig 3D,E) raise questions about the flexibility of helix of E coli DnaG-C in solution Structure verification of helix of DnaG-C An ... side chains of Lys447, Lys448, Ile530 and His541 (C) Domain-swapped dimer in the crystal structure of DnaG-C [4] Only residues 447–528 of conformer I and residues 527–580 of conformer II of the crystal...

Ngày tải lên: 16/03/2014, 12:20

... vector for E coli pNCO113 with the phr gene of E coli pNCO113 with the phr gene of E coli with mutation Glu109Ala expression vector for B subtilis p602-CAT with the phr gene of E coli with mutation ... at an elucidation of a detailed molecular picture of the light-driven DNA repair process Results Construction of a DNA photolyase E109A mutant MTHF, the second cofactor of E coli DNA photolyase, ... selective reaction of KMnO4 Nucleic Acids Research 26, 3940–3943 49 Zamenhof PJ & Villarej M (1972) Construction and properties of Escherichia coli strains exhibiting alphacomplementation of beta-galactosidase...

Ngày tải lên: 16/03/2014, 18:20

... histidine 90 of E coli IIAGlc [27,28] Overexpression and purification of S coelicolor IIACrr and IIACrr(H72A) To study the function of IIACrr, we overexpressed three crr alleles in E coli encoding ... rate-limiting amounts of purified His-tagged IIACrr of S coelicolor and His-tagged IIAGlc of E coli The initial phosphorylation rates of methyl a-glucoside were 152 ± 22 nmol aMG-PÆmin)1 when IIACrr was ... pFT44 The expression plasmid pCRL13 for the production of a C-terminal Histagged IIAGlc of E coli was derived by cloning of an NdeI– HindIII fragment into plasmid pET23a(+) (Novagen) [22] The...

Ngày tải lên: 17/03/2014, 23:20

... uridylylation of the fractional PII* PII*total is shown because the transient uridylylation of the fractional PII* PII*total after removal of ammonia was independent of the PII* concentration ... concentration of the latter (Km ( ẵPII WT ) To examine this possibility, the uridylylated fraction of PII*, i.e PII* UMP PII*total, was measured in the same samples used to measure the deadenylylation of ... absence of ammonia: GlnB versus GlnK Above we measured the primary effect of modulating the expression of glnB in terms of the concentration of PII*, which corresponds to the sum concentration of...

Ngày tải lên: 23/03/2014, 04:21

... of mutations that conferred increased solubility were noted Screening for the boundary of the C-terminal AMPP domain N-terminal deletions of AMPP were generated by exonuclease III digestion of ... crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase P insights into substrate recognition and the mechanism of catalysis Biochemistry ... formed soluble microaggregates of partially unfolded protein (Table 2) Evolution of the AMPP#3-22 fragment – optimizing the starting point Exonuclease III digestion of the DNA corresponding to the...

Ngày tải lên: 23/03/2014, 07:20

... specificity of hydrolytic activity, thus avoiding the synthesis of many different oligosaccharide substrates Previously, we reported that the functionally unknown protein YicI of Escherichia coli was ... initial rate of fluoride-ion liberation for each acceptor The rate of formation of the glycosyl– enzyme intermediate, i.e the rate of fluoride-ion liberation, is governed by the breakdown of the intermediate ... structure of a-d-Xylp-(1 fi 6)-dFruf, the transxylosylation product from fructofuranose, the 4-OH of the fructofuranose would interact with Arg446 and Asp185 at subsite +1 In the case of fructopyranose...

Ngày tải lên: 23/03/2014, 07:20

... between CII and CIII for interaction with HflB also influences the proteolysis of CII [11], because CIII itself is a substrate of HflB [12] CIIIC, the central region of CIII, is not a substrate of HflB, ... digestion of CII by HflB, than CIII [11] Is this mode of antiproteolytic action of CIII a general mode of CIII activity, or does it apply only for CII? We examined the mode of the inhibitory action of ... this respect, r32 resembles kCII Inhibition of HflB-mediated proteolysis of r32 by CIII and by CIIIC The effect of CIII and CIIIC on the HflB-mediated proteolysis of r32 was checked by treating...

Ngày tải lên: 23/03/2014, 10:20

... The effect of PPase concentration on c½ was moderate, in that the latter increased to 1.70 ± 0.09 mM at 0.08 mgÆmL)1 PPase Specificity of the aggregation Fig Time-course of Escherichia coli inorganic ... purification of PPase, with an increase of specific activity from 26 to 150 IUÆmg)1, and a yield of 90% Thus, only small amounts of other proteins in the E coli extracts were co-precipitated with ... No aggregation was observed in the presence of the PPase cofactors Mg2+ and Mn2+ and the PPase inhibitor Ca2+, all of which bind to PPase The nature of the anionic counterion (acetate or chloride)...

Ngày tải lên: 23/03/2014, 13:20