rnase iv of escherichia coli

... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2 (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the absence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of...

Ngày tải lên: 18/02/2014, 18:20

... native form of Hfq has an apparent molecular mass of 50 ± 10 kDa, while the unfolded state of Hfq has an average apparent molecular mass of 12 ± kDa This indicates that the unfolded state of ... binding of the polyadenylated rpsO RNA We have shown that the presence of the remainder of the acidic tail results in the thermodynamic stabilization of Hfq by 1.8 kcalÆmol)1 A comparison of the ... destabilize the network of hydrogen bonds The crystal structure of the first 71 amino acids of E coli Hfq indicates that the C-terminal domain is probably located at the top of the ring, because...

Ngày tải lên: 19/02/2014, 12:20

... The radioactivity of gel slices was determined by Cerenkov counting The yield of the complex was calculated as the ratio of shifted band radioactivity to the total radioactivity of the loaded ... mobilities of the radioactive and the protein-containing bands indicated covalent attachment of DNA to the enzyme The yield of the photochemical cross-linking reaction was calculated as the ratio of ... radioactivity to the total radioactivity of the conjugate and unbound DNA 1 Ó FEBS 2003 Cross-linking E coli Fpg protein to DNA duplexes (Eur J Biochem 270) 2947 Results and discussion Design of...

Ngày tải lên: 20/02/2014, 11:20

... key aspects of functionality and regulation Nature 420, 190–193 44 Bachmann, B.J (1996) Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 In Escherichia coli and Salmonella: ... expression of the fumarase gene of Escherichia coli K-12 J Bacteriol 153, 588–596 31 Model, P & Rittenberg, D (1967) Measurement of the activity of the hexose monophosphate pathway of glucose ... bound of 80% PEP from P5P (Fig 6) The PfkA mutant is deficient in the allosterically regulated, major isoform of phosphofructokinase that constitutes about 90% of the total phosphofructokinase activity...

Ngày tải lên: 20/02/2014, 23:20

... superimposition of the structures of B caldovelox arginase (red), rat liver arginase (yellow) and the modelled structure of E coli agmatinase (blue) Note the shorter extension, in the case of E coli agmatinase, ... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... D153N mutant form of E coli agmatinase was obtained The mutant enzyme retained about 5% of wild-type activity and it was equally active when assayed in the presence or absence of Mn2+ However,...

Ngày tải lên: 21/02/2014, 01:21

... (B) Activation of the acetyl phosphate synthesis activity of E coli Pta by different concentrations of pyruvate and PEP Results are presented as percentage of activity in the presence of PEP ... al Escherichia coli phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli ... concentration of this compound in E coli growing on glucose [14] Thus, although E coli Pta is more active in the direction of acetyl-CoA synthesis (Table 1), the in vivo concentrations of Pta substrates...

Ngày tải lên: 06/03/2014, 11:20

... superimposition of fold type I enzymes reveals that the location of the cofactor in the active site is virtually identical in all members of the group [7] Despite the high similarities of their 3D ... negative band at approximately 340 nm, which are similar in all enzyme forms (data not shown) The presence of the cofactor negative band determines a significant increase of the overall negative ... which showed how the native conformation of the loop is pivotal to PLP binding and catalysis [22] Given the above considerations, a series of possible, correlated outcomes of the mutations may be...

Ngày tải lên: 07/03/2014, 03:20

... epidemiology of Escherichia coli in broiler breeder chickens Avian Pathology 3, 591–605 Griffin PM (1995) Escherichia coli 0157: H7 and other enterohemorrhagic Escherichia coli In: Infections of the ... Characterization of Escherichia coli isolated from cases of avian colibacillosis Canadian Journal of Veterinary Research 57, 146–151 Allen DG, Pringle X, Smith D, Conlon PD & Burgmann PM (1993) Handbook of ... pathogens: Escherichia coli 0157: H7 as a model of entry of a new pathogen into the food supply of the developed world Epidemiological Review 18, 29–51 Aronson AL (1975) Potential impact of the use of...

Ngày tải lên: 08/03/2014, 09:20

... phoE pJP29 derivative encoding (G-10R)prePhoE pJP29 derivative encoding (G-10L)prePhoE Ampr, phoE gene behind tac promoter pNN100 derivative encoding (G-10R)prePhoE pNN100 derivative encoding ... composition of the outer membrane proteins of Escherichia coli Mol Gen Genet 147, 251–262 35 Bosch, D., de Boer, P., Bitter, W & Tommassen, J (1989) The role of the positively charged N-terminus of the ... accumulation of truncated forms of outer-membrane PhoE protein of Escherichia coli K-12 J Mol Biol 189, 449–455 34 Lugtenberg, B., Peters, R., Bernheimer, H & Berendsen, W (1976) Influence of cultural...

Ngày tải lên: 08/03/2014, 09:20

... structure of E coli R3 and R4 LPS (Eur J Biochem 269) 5983 Lipopolysaccharide (LPS) is the major component of the outer leaflet of the outer membrane of Gram-negative bacteria [1] LPS of enterobacteria ... in LPS from all core-types of E coli and Salmonella Investigation of the inter-residual NOE connectivities revealed that no long range NOEs indicative of a backfolding of the outer core were found ... HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, the deacylated minor core-oligosaccharide of E coli F653...

Ngày tải lên: 08/03/2014, 09:20

... temperatures until completion of the reaction The extent of refolding was calculated from the recovered activity relative to the activity of the same amount of native protein Acknowledgements ... percentage recovery of MalZ activity in different samples was calculated after considering the equivalent amount of native MalZ activity as 100% The spontaneous refolding of MalZ was approximately ... 55 58 66 % Relative MalZ activity 100 80 60 40 20 0 [urea] (M) Fig Deactivation of MalZ (5 lM) by increasing concentration of urea in the presence of an optimized concentration of TMAO (.), glycerol...

Ngày tải lên: 16/03/2014, 06:20

... constant of inactivation by TPCK-trypsin at C was found to be around ã 10)3ặs)1 Addition of ATP protected all modied CMP kinases, decreasing the rst-order rate constant of inactivation by a factor of ... reaction Analysis of the effect of the mutations on protein stability revealed three distinctive mechanisms of relaxation, and in the case of S36A, no relaxation at all The rst type of relaxation ... from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein...

Ngày tải lên: 16/03/2014, 10:20

... helix of E coli DnaG-C observed in the crystal structure (Fig 3D,E) raise questions about the flexibility of helix of E coli DnaG-C in solution Structure verification of helix of DnaG-C An extensive ... rmsd of 3.2 A for 88 aligned residues from the globular part of P16 (corresponding to residues 449–543 of E coli DnaG-C), which excludes helices and of P16 (Fig 1) Helices 5002 Fig Stereo views of ... E coli DnaG-C [4] (E) Conformer II of the crystal structure dimer of E coli DnaG-C [4] structure of DnaG-C as a domain-swapped dimer, where helix from one protein molecule binds to the core of...

Ngày tải lên: 16/03/2014, 12:20

... at an elucidation of a detailed molecular picture of the light-driven DNA repair process Results Construction of a DNA photolyase E109A mutant MTHF, the second cofactor of E coli DNA photolyase, ... Photoactivation of the catalytically blue radical form of DNA photolyase Overexpression strains of E coli can generate large amounts of recombinant DNA photolyase in the catalytically active dihydroflavin ... vector for E coli pNCO113 with the phr gene of E coli pNCO113 with the phr gene of E coli with mutation Glu109Ala expression vector for B subtilis p602-CAT with the phr gene of E coli with mutation...

Ngày tải lên: 16/03/2014, 18:20

... found upstream of ptsI In this communication we provide evidence that this putative crr gene is expressed in vivo and that it constitutes a functional equivalent of its homologue in E coli MATERIALS ... digested with the same enzymes giving pFT44 The expression plasmid pCRL13 for the production of a C-terminal Histagged IIAGlc of E coli was derived by cloning of an NdeI– HindIII fragment into ... which cell extracts of LM1 were combined with rate-limiting amounts of purified His-tagged IIACrr of S coelicolor and His-tagged IIAGlc of E coli The initial phosphorylation rates of methyl a-glucoside...

Ngày tải lên: 17/03/2014, 23:20

... property of interest, e.g ux through the pathway (J), divided by the relative (small) modulation of the effector concentration gives the uxresponse coefcient (RJ ), whereas the A effect of A on ... structure of the GS adenylylation system We observed that the pivotal regulatory protein GlnB does not control the steady-state activity of GS Its control of the deadenylylation rate of GSAMP ... quantitatively in vivo and under two relevant growth conditions, whether signal transduction from ammonia depletion to GSAMP deadenylylation is highly sensitive to the concentration of the pivot of...

Ngày tải lên: 23/03/2014, 04:21

... coordinate the two divalent metal ions in the active site of AMPP (Fig 4) The positively charged metals will neutralize the negatively charged acids In the absence of metals, the negatively charged ... given to the starting point of the AMPP C-terminal domain as well as its C-terminal domain of E coli aminopeptidase P sequence The location of the domain boundary was estimated by inspection of ... crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase P insights into substrate recognition and the mechanism of catalysis Biochemistry...

Ngày tải lên: 23/03/2014, 07:20

... presence of 10 mm of each transxylosylation product 6078 Relative a-glucosidase activity, taking activity in the absence of the transxylosylation product as 100% for each substrate, is shown in Fig Of ... 6077 Transxylosylation of YicI M.-S Kang et al Fig Histograms of relative a-glucosidase activity in the presence of each disaccharide transxylosylation product when the activity without transxylosylation ... specificity of hydrolytic activity, thus avoiding the synthesis of many different oligosaccharide substrates Previously, we reported that the functionally unknown protein YicI of Escherichia coli was...

Ngày tải lên: 23/03/2014, 07:20

... activity of kCIII, an inhibitor of the Escherichia coli metalloprotease HflB (FtsH) J Bacteriol 189, 8130–8138 Herman C, Thevenet D, D’Ari R & Bouloc P (1997) The HflB protease of Escherichia coli ... Is this mode of antiproteolytic action of CIII a general mode of CIII activity, or does it apply only for CII? We examined the mode of the inhibitory action of CIII on proteolysis of the heat ... Induction of the heat shock response of E coli through stabilization of r32 by the phage kCIII protein Genes Dev 1, 57–64 Kornitzer D, Altuvia S & Oppenheim AB (1991) The activity of CIII regulator of...

Ngày tải lên: 23/03/2014, 10:20

... in a sixfold purification of PPase, with an increase of specific activity from 26 to 150 IUÆmg)1, and a yield of 90% Thus, only small amounts of other proteins in the E coli extracts were co-precipitated ... The effect of PPase concentration on c½ was moderate, in that the latter increased to 1.70 ± 0.09 mM at 0.08 mgÆmL)1 PPase Specificity of the aggregation Fig Time-course of Escherichia coli inorganic ... the native tertiary structure is regained when the Cd2+ is removed The dependence of aggregation on Cd2+ concentration was characteristic of a highly cooperative process (Fig 2) Aggregation of pyrophosphatase...

Ngày tải lên: 23/03/2014, 13:20