mara soxs and rob regulons of escherichia coli

... New Tool for Typing Genetic Variants of the LEE Pathogenicity Island of Human and Animal Shiga Toxin-Producing Escherichia coli (STEC) and Enteropathogenic E coli (EPEC) Strains Clin Chem 2006, ... C-terminal domains of intimin-like proteins of enteropathogenic and enterohemorrhagic Escherichia coli, Citrobacter freundii, and Hafnia alvei Infect Immun 1994, 62(5):1835-42 Hartland EL, Batchelor ... proteins) for vaccination of healthy cattle as an aid in reducing shedding of Escherichia coli O157: H [44] Both of these vaccines are high risk and are insufficiently safe and for this reason we...

Ngày tải lên: 13/08/2014, 16:20

... understand more about PapI and how to better work with it 19 Purification and Characterization of Lrp and PapI 2.1 Purification and characterization of Lrp 2.1.1 Materials and Methods Purification of ... hand corner of the graph and proceeds to the right The unmelting curve begins at the top right hand corner of the graph and proceeds to the left 30 Purification and Characterization of Lrp and ... concentration of 25 mg/ml Six liters of cells yielded about 50 mg of Lrp 20 Purification and Characterization of Lrp and PapI Biochemical characterization of Lrp To determine if the Lrp produced and purified...

Ngày tải lên: 14/11/2014, 06:09

... of ionic strength and Mg2+ on the activity and specificity of OGG1, we measured the apparent values of k2 and k3 under conditions of low salt (KPi only) and no Mg2+, low salt and 20 mm Mg2+, and ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KCl and MgCl2 (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1 The extent of cleavage ... Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G–I) AP lyase activity of OGG1...

Ngày tải lên: 18/02/2014, 18:20

... positions of other Fig Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N mutant forms of E coli ... superimposition of the structures of B caldovelox arginase (red), rat liver arginase (yellow) and the modelled structure of E coli agmatinase (blue) Note the shorter extension, in the case of E coli agmatinase, ... ligand-manganese distances RESULTS AND DISCUSSION By site-directed mutagenesis, a D153N mutant form of E coli agmatinase was obtained The mutant enzyme retained about 5% of wild-type activity and...

Ngày tải lên: 21/02/2014, 01:21

... on the biochemical characterization of E coli Pta and set out to investigate the function of its N-terminal domain by the construction and analysis of three E coli Ptas with deletions from the ... phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta, three conserved domains ... compilation ª 2010 FEBS 1959 Escherichia coli phosphotransacetylase V A Campos-Bermudez et al Fig Comparative CD spectra of E coli Pta and truncated Ptas CD spectra of Pta, Pta-F1 and Pta-F2 were recorded...

Ngày tải lên: 06/03/2014, 11:20

... Standards (NCCLS 1993) In addition, E coli organisms isolated from chicken and Table Comparison of pattern of resistance of E coli isolates from chicken and patients E coli- chicken isolates E coli- patient ... isolates Serotyping of 119 and 100 E coli organisms isolated from chicken and patients, respectively, is presented in Table Only 30.3% of chicken E coli isolates and 38% of patient isolates were ... Characterization of Escherichia coli isolated from cases of avian colibacillosis Canadian Journal of Veterinary Research 57, 146–151 Allen DG, Pringle X, Smith D, Conlon PD & Burgmann PM (1993) Handbook of...

Ngày tải lên: 08/03/2014, 09:20

... structure of E coli R3 and R4 LPS (Eur J Biochem 269) 5983 Lipopolysaccharide (LPS) is the major component of the outer leaflet of the outer membrane of Gram-negative bacteria [1] LPS of enterobacteria ... assignment of H-7a/b and C-7 of L,D-Hepp residues F and H The chemical shift of H-6 of residue F could also be assigned in the latter spectrum The chemical shift of the H-6 proton of residue ... of anomeric protons and two pairs of signals originating from 3-deoxy protons characteristic of aKdop The assignment of H and 13C-resonances (Tables and 3) and determination of vicinal coupling...

Ngày tải lên: 08/03/2014, 09:20

... Overproduction and molecular characterization of the modied variants of E coli CMP kinase The wild-type and various modied forms (S36A, R110M, D132A, D132H, D132N, D132S and R188M) of E coli CMP kinase ... Comparative structures of CMP and UMP, and interactions between the cytosine moiety of nucleotide and various side chains of wild-type CMP kinase (Upper) Chemical differences between CMP and UMP are indicated ... from the distal point of the rest of the protein Such a dipole will have weak interactions with the rest of the protein Thus, if turned off (by removal of Arg and protonation of Asp185), the protein...

Ngày tải lên: 16/03/2014, 10:20

... histidine 75 and the experimentally proven phosphorylation site histidine 90 of E coli IIAGlc [27,28] Overexpression and purification of S coelicolor IIACrr and IIACrr(H72A) To study the function of IIACrr, ... volume of 0.1 mL containing rate-limiting amounts of IIA protein (50 pmoles), 55 lg protein of LM1 extract, and a final concentration of 12 lM [14C]aMG (1.4 mCiÆmmol)1) Phosphorylation of aMG ... optical biosensor (Biacore AB) Three micrograms of S coelicolor Histagged IIACrr (180 pmoles), of E coli His-tagged IIAGlc (150 pmoles), and of E coli His-tagged tetracycline repressor TetR (125...

Ngày tải lên: 17/03/2014, 23:20

... the GlnB band was quantied as the sum of the concentrations of GlnB and GlnK and was denoted by PII* Note that PII* includes the modied forms of GlnB and GlnK as well, i.e GlnBUMP and GlnKUMP ... transient uridylylation of PII* and the transient deadenylylation of GSAMP See also Figs and Cells had been grown in the presence of ammonia Uridylylation of PII* and deadenylylation of GSAMP were measured ... Scanning of the autoradiograms and the determination of intensity of the PII* and PII*UMP bands were as described above Some gels did not result in a complete separation of the uridylylated and native...

Ngày tải lên: 23/03/2014, 04:21

... Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase P insights into substrate recognition and the mechanism of catalysis ... digestion of the pepP gene A set of nested truncated pepP genes was fused to that of DHFR in the fusion vector pJWL1030folA and transformed into competent E coli cells Two libraries of about 10 ... number of mutations per gene The implication of this observation is that the effects of most mutations are small compared with those of G270V, E406G, and R166G Changes at the surface of the protein...

Ngày tải lên: 23/03/2014, 07:20

... behavior of wild-type and a subset of the mutants of FrdABCD in DW35 membranes The wild-type enzyme has a Km for LPCH2 of approximately 225 lm and a kcat of approximately 71 s)1 The FrdB-T205H and ... structure of FrdABCD: T205, F206, Q225 and K228 from FrdB; R28, E29, W86, L89 and A93 from FrdC; and W14, F17, G18, H80, R81 and H84 from FrdD [3,4,10] Site-directed mutants of some of these residues ... spin-coupled pair of [4Fe-4S] clusters and comprises a peak at g ¼ 2.29, and troughs at g ¼ 1.87 and 1.67 Addition of HOQNO causes the appearance of a peak at g ¼ 1.98 (compare Figure 4Ci and ii) Overall,...

Ngày tải lên: 23/03/2014, 13:20

... form in aerobic and in anaerobic apoFNR (Table 3) The 23 ⁄ 29 Table Quantitative evaluation of thiol and disulfide containing peptides of aerobically and anaerobically prepared apoFNR Aerobically ... 1) The residues 4261 Disulfides of apoFNR Fig MALDI-TOF spectra of aerobically (A) and anaerobically (B) prepared and carboxymethylated apoFNR The samples of apoFNR were alkylated with 10 mM iodoacetate ... fivefold alkylated apoFNR, and a minor signal of threefold alkylated FNR (Fig 1B) Therefore, aerobic and anaerobic apoFNR are mixtures of FNR with one, three and five, and five and three cysteine...

Ngày tải lên: 23/03/2014, 15:20

... inactivation of wild-type CTPS by trypsin (A) Inactivation of CTPS-catalysed NH3-dependent CTP formation in the absence of ligands (s) and in the presence of ATP (10 mM, n), UTP (10 mM, h), and ATP and ... treatment of CTPS with trypsin produced a limited number of cleavage fragments over the course of h, of which the formation of some fragments was suppressed in the presence of ATP and/ or UTP ... of mutant CTP synthases Fig SDS/PAGE analysis of trypsin-catalysed cleavage of recombinant wild-type CTPS in the absence and presence of ligands For each gel: lane contains molecular mass standards...

Ngày tải lên: 23/03/2014, 17:22

... mechanisms of membrane anchoring of Escherichia coli penicillin binding proteins FEMS Microbiol Rev 13, 1–12 Ehlert, K & Holtje, J.-V (1996) Role of precursor translocation in coordination of murein and ... sequences of the penicillin-binding protein and genes of Escherichia coli Nucleic Acids Res 16, 1617 Schiffer, M & Edmundson, A.B (1967) Use of helical wheels to represent the structures of proteins and ... temperature (Figs and 6) Control experiments recorded the Tc of both Myr2-PGro and Myr2PCho membranes as 25 °C and that of Myr2-PEtn membranes as 47 °C (Figs 3A–C and 6A)C) In the presence of P4, no...

Ngày tải lên: 31/03/2014, 08:20

... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... (10/1,682) 0.65 (11/1,682) a No of positive/No of samples examined No of negative/No of samples examined b Table Antibiotic resistance profiles of isolated E coli O157:H7 Antimicrobial discs Resistant ... results of CPE of eleven E coli O157:H7 isolates were shown in Table Antimicrobial susceptibility disc tests A total of 23 antimicrobial discs were used in this study Five of eleven E coli O157:H7...

Ngày tải lên: 07/08/2014, 18:21

... noitacifilpma eht ni deyolpme stes remirp ehT nim rof C 27 ta pets noisnetxe na dna ,ces 54 rof C 26 ta gnilaenna ,ces 54 rof C 49 ta noitarutaned :snoitidnoc gniwollof eht rednu ,)KU ,diabyH omrehT( sserpxE ... locinehpmarolhc eht fo eno gnirobrah osla )%6.82( setalosi eseht fo 02 htiw ,eneg Rolf eht rof evitisop erew )%6.81( setalosi iloc E neetriht dna eneg Almc eht rof evitisop eb ot dnuof erew )%4.12( setalosi ... ,)sLCCN ylmrof( etutitsnI sdradnatS yrotarobaL dna lacinilC ,2A-13M ISLC de dn2 dradnatS devorppA ;slaminA morf detalosI airetcaB rof stseT ytilibitpecsuS noituliD dna ksiD laiborcimitnA rof sdradnatS...

Ngày tải lên: 07/08/2014, 20:23

... numbers are: Escherichia coli CFT073 [GenBank:AE014075]; Escherichia coli K-12 MG1655 [GenBank:U00096]; Escherichia coli O157:H7: RIMD0509952 (Sakai) [GenBank:BA000007]; Escherichia coli O157:H7: ... as 6-tuples of ones and twos (for allele and allele 2) in the following order: (K) E coli K-12 MG1655, (O) E coli O157:H7 EDL933, (O) E coli O157:H7 Sakai strain RIMD0509952, (C) E coli CFT073, ... sequence of uropathogenic Escherichia coli Proc Natl Acad Sci USA 2002, 99:17020-17024 Stoltzfus A, Leslie JF, Milkman R: Molecular evolution of the Escherichia coli chromosome I Analysis of structure...

Ngày tải lên: 14/08/2014, 16:21

... the emergence of Enterohemorrhagic Escherichia coli: an evolutionary and functional analysis FEMS Microbiol Lett 2007, 273:58-63 Bohm H, Karch H: DNA fingerprinting of Escherichia coli O157:H7 ... The use of product and company names is necessary to accurately report the methods and results; however, the USDA neither guarantees nor warrants the standard of the products, and the use of Genome ... sequence of enterohaemorrhagic Escherichia coli O157:H7 Nature 2001, 409:529-533 Feng P, Lampel KA, Karch H, Whittam TS: Genotypic and phenotypic changes in the emergence of Escherichia coli O157:H7...

Ngày tải lên: 14/08/2014, 21:20

... recovery of E coli ATCC 11229 and ATCC 15597 following h of fluorescent light and sunlight exposure with LP and MP UV disinfection 126 Table 5-2 Comparison of repair rates of E coli ATCC 11229 and ... 2.9.5 Dark repair 58 UV Disinfection and DNA Repair of Escherichia coli 59 2.10.1 UV disinfection of E coli 59 2.10.2 DNA repair of E coli 61 CHAPTER MATERIALS AND METHODS 66 3.1 Overview 66 3.2 ... for Escherichia coli strains ATCC 15597 and ATCC 700891 following LP and MP UV disinfection 99 Table 4-3 Dark repair data for Escherichia coli strains ATCC 15597 and ATCC 700891 following LP and...

Ngày tải lên: 11/09/2015, 16:03