rnases i ii and iv of escherichia coli

... a No of positive/No of samples examined No of negative/No of samples examined b Table Antibiotic resistance profiles of isolated E coli O157:H7 Antimicrobial discs Resistant (%) Intermediate ... O’Brien AD Structure, biology, and relative toxicity of Shiga toxin family members for cells and animals Escherichia coli O157:H7 and Shiga toxinproducing E coli strains American Society for Microbiology, ... Yamasaki S, Takeda Y, Nair GB Antibiotic resistance, virulence gene, and molecular profiles of Shiga toxin- producing Escherichia coli isolates from diverse sources in Calcutta, India J Clin Microbiol...

Ngày tải lên: 07/08/2014, 18:21

... efficiency of UV disinfection is reduced and this is particularly significant when visible light exposure following UV disinfection is involved Reactivation of bacteria following UV disinfection ... 4.2 UV Inactivation of E coli 90 4.3 Photoreactivation of E coli following UV Disinfection 94 4.4 Dark Repair of E coli following UV Disinfection 100 4.5 Comparison of Repair of Selected Indicators ... possible i TABLE OF CONTENTS Page ACKNOWLEDGEMENTS i TABLE OF CONTENTS ii SUMMARY vii NOMENCLATURE x LIST OF TABLES xii LIST OF FIGURES xiii CHAPTER INTRODUCTION 1.1 Disinfection in Drinking Water Treatment...

Ngày tải lên: 11/09/2015, 16:03

... Aprotinin Conclusion Re MCoTI -II 4 Fig 14 Checking the inhibiting activity of ReMCoTI -II by using diffusion method PA from S litula PA of S litula + ReMoTI -II Trypsin Trypsin + ReMCoTI -II Conclusion ... TGAAGCGCCA 480 Fig Sequence of recombinant plasmid DNA containing TI gene fragment Expression of recombinant MCoTI -II (ReMCoTI -II) The expression conditions such as ampicillin and IPTG concentrations, ... (Fig 13) ReMCoTI showed inhibitory activity against trypsin as well as proteinase from Spodoptera litura (Fig 14) Fig 13 Checking the purity and size of ReMCoTI -II by SDS PAGE Maker 2, Aprotinin...

Ngày tải lên: 12/02/2014, 10:20

... Significance of error-avoiding mechanisms for oxidative DNA damage in carcinogenesis Cancer Sci 98, 465–470 Wilson DM III & Bohr VA (2007) The mechanics of base excision repair, and its relationship to ... of the apurinic ⁄ apyrimidinic (AP) site via elimination of its 3¢-phosphate (AP lyase activity) after excision of the damaged base (DNA glycosylase activity) Fpg efficiently catalyzes b,d-elimination ... Fig Activity and specificity of Fpg and OGG1 in buffers with different concentrations of KGlu and MgCl2 in the presence of KPi (A–C) Fpg, (D–F) glycosylase activity of OGG1, (G I) AP lyase activity...

Ngày tải lên: 18/02/2014, 18:20

... because of their implications in the metabolism of ethanol and retinoic acid In vitro and in vivo data indicate that ADH1, ADH3 and ADH4 can oxidize retinol to retinal, with ADH4 having very high ... dehydrogenase activity in the human tissues Biochem Med 31, 1–9 Jelski, W., Chrostek, L., Szmitkowski, M & Laszewicz, W (2002) Activity of class I, II, III, and IV alcohol dehydrogenase isoenzymes in human ... significant involvement of ADH1 and ADH4 in ethanol oxidation in brain tissue Regarding retinoid metabolism in the adult brain, enzymes other than ADH4 must be active, because in vivo and in vitro...

Ngày tải lên: 21/02/2014, 00:20

... Crystal structures of Bacillus caldovelox arginase in complex with substrate and inhibitors reveal new insights into activation, inhibition and catalysis in the arginase superfamily Structure 7, ... putrescine biosynthetic enzyme in Escherichia coli J Bacteriol 165, 843–848 Buch, J.K & Boyle, S.M (1985) Biosynthetic arginine decarboxylase in Escherichia coli is synthesized as a precursor and ... agmatinase and arginase, agmatine and arginine were fixed in essentially the same position in the corresponding active site The same position for the scissile guanidine carbon of the substrates, with...

Ngày tải lên: 21/02/2014, 01:21

... N-terminal domain is involved in stabilization of the hexameric native structure, in expression of the maximum catalytic activity, and in allosteric regulation by NADH, ATP, pyruvate, and phosphoenolpyruvate ... activity This differential Pta activity regulation by pyruvate and PEP may be important in vivo, as this enzyme is involved in balancing pyruvate flux when E coli grows on rich medium, by opting ... complementation with Pta-F3 (Fig 6), which displays very low activity and is not regulated by metabolites at all It is thus possible that the metabolic regulation of E coli Pta is Escherichia coli phosphotransacetylase...

Ngày tải lên: 06/03/2014, 11:20

... industry and/ or for patients in E coli- chicken ———————– Drugs Ampicillin, chloramphenicol Ampicillin, gentamicin Ampicillin, spectinomycin Ampicillin, tetracycline Ampicillin, TMPϩSMX Ampicillin, ... Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain Journal of Clinical Microbiology ... resistance rates to individual drugs in E coli isolated from patients remained significantly lower than those of E coli chicken isolates with the exception of resistance to ceftazidime, AMX and...

Ngày tải lên: 08/03/2014, 09:20

... a limited structural variability within the same species This prompted many investigators to attempt the isolation of antibodies directed against the conserved regions of LPS, i. e lipid A and ... negative ion mode of the mixture of oligosaccharides prior to HPAEC, of the isolated main oligosaccharide of deacylated LPS and of acylated purified LPS from E coli F2513 (R4 core-type) In addition, ... 10 signals of anomeric protons and two pairs of signals originating from 3-deoxy protons characteristic of aKdop The assignment of H and 13C-resonances (Tables and 3) and determination of vicinal...

Ngày tải lên: 08/03/2014, 09:20

... shown in Table 2, introduction of a H-bond donor via Asn substitution of D132 is not compatible with enzyme binding and substrate catalysis Kinetic and nucleotide-binding properties of E coli CMP ... A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding Anal Biochem 72, 248254 30 Laemmli UK (1970) Cleavage of structural ... involved in the interaction with the phosphate moieties of various NMPs (R41, R131 and R181 in E coli CMP kinase) and the four modied residues of E coli CMP kinase specically interacting with the...

Ngày tải lên: 16/03/2014, 10:20

... DNA-polymerase, and HindIII giving plasmids pFT111 (his-tagged IIACrr) and pFT112 (IIACrr) [24] Protein overproduction and purification Recombinant His-tagged HPr from S coelicolor, Histagged IIACrr from ... phosphorylation site histidine 90 of E coli IIAGlc [27,28] Overexpression and purification of S coelicolor IIACrr and IIACrr(H72A) To study the function of IIACrr, we overexpressed three crr alleles in ... inducer exclusion function of E coli IIAGlc by inhibition of maltose uptake might be a good indication for this hypothesis The demonstration of the IIACrr–MalK interaction suggests that IIACrr may...

Ngày tải lên: 17/03/2014, 23:20

... Semidry blotting, probing and visualization of (uridylylated) PII* was performed as described previously [33] Scanning of the autoradiograms and the determination of intensity of the PII* and PII*UMP ... [59] and ligated into pFC13 digested with BamHI and SspI, resulting in pWVH112; and (b) inserting a linker containing the restriction sites SI, NotI and BssHII with on both ends sticky BamHI sites ... polymerase I) of pBIISK (see Fig for its orientation) (b) NotI linker (5Â-AGC GGCCGCT-3Â; New England Biolabs, Ipswich, MA, USA) was ligated into the HincII site of the pBIISK polylinker (c) One linker...

Ngày tải lên: 23/03/2014, 04:21

... exonuclease III digestion and subsequent screening by fusing them to the DHFR reporter gene and selecting with TMP The initial round of truncations gave a series of deletions that allowed cells to survive ... isomerase is an example of a protein that requires a metal to fold It requires zinc ions for both in vivo and in vitro folding [22] The in vitro folding studies described in this article suggest ... the incoming metals Four acid residues coordinate the two divalent metal ions in the active site of AMPP (Fig 4) The positively charged metals will neutralize the negatively charged acids In the...

Ngày tải lên: 23/03/2014, 07:20

... RB (1998) Localization of histidine residues responsible for heme axial ligation in cytochrome b556 of complex II (succinate: ubiquinone oxidoreductase) in Escherichia coli Biochemistry 37, 4148–4159 ... Yankovskaya V, Cecchini G & Iwata S ¨ (2002) Purification, crystallisation and preliminary crystallographic studies of succinate: ubiquinone oxidoreductase from Escherichia coli Biochim Biophys Acta 1553, ... Ramsay RR, Singer TP, Ackrell BAC, Cecchini G & Miyoshi H (1996) Inhibitor probes of the quinone binding sites of mammalian complex II and Escherichia coli fumarate reductase J Biol Chem 271,...

Ngày tải lên: 23/03/2014, 13:20

... [32] and the radioactivity was measured in a scintillation counter Radioactivity was corrected for not incorporated radioactivity by measuring and subtracting the radioactivity in identically ... [4Fe-4S]ÆFNR in vitro by labeling of cysteinyl thiol residues with iodoacetate after denaturing the protein Kinetics of cysteinyl residue oxidation during inactivation of [4Fe-4S]ÆFNR by air Reconstituted ... indicative for disulfides were found (Fig 5), suggesting that up to two disulfides are formed by air-inactivation of FNR in vitro This again shows that the air-inactivated FNR is a mixture of apoFNR...

Ngày tải lên: 23/03/2014, 15:20

... relative to wildtype CTPS Thus mutation of Arg429 to alanine impairs both GTP binding and allosteric activation of glutamine hydrolysis Comparison of the kcat values for the glutaminase activity and ... T-lymphoblastic cell line Int J Cancer 98, 616623 Gao, W.Y., Johns, D.G & Mitsuya, H (2000) Potentiation of the anti-HIV activity of zalcitabine and lamivudine by a CTP synthase inhibitor, 3-deazauridine ... site Competitive inhibition experiments have suggested that for E coli CTPS, this site may also be the UTP-binding site [18] The locations of the ATP- and GTPbinding sites have not yet been identied...

Ngày tải lên: 23/03/2014, 17:22

... Harris, F., Phoenix, D.A & Seydel, U (2001) An FTIR investigation into the lipid interactions of peptides corresponding to the C-terminal anchoring regions of Escherichia coli penicillin-binding ... penicillin-binding proteins 4, and Biochim Biophys Acta 1329, 278–284 Jackson, M.E & Pratt, J.M (1988) Analysis of the membranebinding domain of penicillin-binding protein of Escherichia coli Mol Microbiol ... Escherichia coli low molecular mass penicillinbinding proteins Thesis University of Central Lancashire, Preston, Lancashire, UK Phoenix, D.A & Harris, F (1998) Amphiphilic a–helices and lipid interactions...

Ngày tải lên: 31/03/2014, 08:20

... to predict the Ab initio comparativechimeric protein, EspA-Intimin-Tir Ab initio and comparative modeling was used to predict the tertiary structure of the chimeric protein, EspA-Intimin-Tir The ... Ramachandran plot and (B) energy minimization from an extract of lysed bacteria containing type III secretion proteins) for vaccination of healthy cattle as an aid in reducing shedding of Escherichia ... Rogan D, Mutwiri GK, Potter AA: Subcutaneous and intranasal immunization with type III secreted proteins can prevent colonization and shedding of Escherichia coli O157:H7 in mice Microb Pathog...

Ngày tải lên: 13/08/2014, 16:20

... the 186 high scoring segments, subdivided into MSCs and identifying affected genes, is provided as Additional data file We examined this list of 311 genes in light of gene function assignments ... phylogenetic partitioning pattern Variability in mutation rates and patterns in different chromosomal regions and bacterial lineages might also lead to physical clustering of similar substitutions Although ... O157:H7 and S flexneri Exactly which pair of lineages is involved in the transfer can sometimes be determined by examining the joint distribution of all seven SND patterns Recombinant activity in...

Ngày tải lên: 14/08/2014, 16:21

... described in this study have an immediate utility in distinguishing genetically distinct STEC O157 strains that appear identical by PFGE profile portion of STEC O157 diversity that appears primarily in ... LM: Shiga-toxigenic Escherichia coli O157 in agricultural fair livestock, United States Emerg Infect Dis 2006, 12:780-786 Whittam TS: Evolution of Escherichia coli O157:H7 and other Shiga toxin-producing ... polymorphisms identified in this study; and PFGE (Additional data file 1) DNA isolation The polymorphisms validated in this study primarily reside in the conserved backbone of E coli and some may be informative...

Ngày tải lên: 14/08/2014, 21:20