rnase i of escherichia coli

... Recognition of oxidatively modified bases within the biotin-binding site of avidin J Mol Biol 357, 263–274 Porta M, Vioque J, Ayude D, Alguacil J, Jariod M, Ruiz L & Murillo JA (2003) Coffee drinking: ... to induce conformational transitions in nucleic acids, possibly by selective stabilization ⁄ destabilization of one of the conformations; for example, submillimolar concentrations of Mg2+ induce ... strand nicking at apurinic ⁄ apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase Biochem J 262, 581–589 Lipanov A, Kopka ML, Kaczor-Grzeskowiak M, Quintana J & Dickerson...

Ngày tải lên: 18/02/2014, 18:20

... contribution of this region to the thermodynamic stability of Hfq Equilibrium unfolding studies of Hfq were performed using protein intrinsic fluorescence Prior to analysis of the denaturation curves, ... proteins, which contain mostly acidic residues In addition, this extension is highly structured in some archaeal Sm-like proteins [31], while it is likely disordered in Hfq However, this C-terminal ... rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Schagger, H & von Jagow, G (1987) Tricine-sodium...

Ngày tải lên: 19/02/2014, 12:20

... duplexes I, II and III, respectively Thus, the binding efficiency of the reactive DNA duplexes I and II was similar to the binding efficiency of DNA duplex III, which has the same sequence but contains ... Gajewski, E., Laval, J & Dizdaroglu, M (1992) Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): excision of purine lesions in DNA produced by ionizing ... Synthesis of a new photo-cross-linking nucleoside analogue containing an aryl(trifluoromethyl)diazirine group: application for EcoRII and MvaI restriction-modification 11 enzymes Nucleosides Nucleotides...

Ngày tải lên: 20/02/2014, 11:20

... profiling from 13C-labeling experiments This methodology is based on probabilistic equations that relate mass distributions in amino acids to metabolic activities, and quantifies the relative contribution ... P & Rittenberg, D (1967) Measurement of the activity of the hexose monophosphate pathway of glucose metabolism with the use of [18O]glucose Variations in its activity in Escherichia coli with ... was thus used in the further experiments Metabolic flux ratio analysis of E coli under different environmental conditions While exponentially growing cells are initially in a physiological pseudo...

Ngày tải lên: 20/02/2014, 23:20

... E coli expression vector and the histidine-tagged enzyme was expressed in E coli strain JM109, following induction with mM isopropyl thio-bD-galactoside The histidine-tagged enzymes were purified ... Crystal structures of Bacillus caldovelox arginase in complex with substrate and inhibitors reveal new insights into activation, inhibition and catalysis in the arginase superfamily Structure 7, ... 507–518 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Ho, S.N.,...

Ngày tải lên: 21/02/2014, 01:21

... domain (Fig 2), are needed for maximal catalytic activity, participating either directly in the catalytic mechanism, or indirectly in the conformation of the catalytic site The oligomeric state of ... incubation at 37 °C Discussion Biochemical properties of E coli Pta in relation to its physiological role In the present work, detailed biochemical characterization of E coli Pta was performed ... the E coli Pta N-terminal domain is involved in stabilization of the hexameric native structure, in expression of the maximum catalytic activity, and in allosteric regulation by NADH, ATP, pyruvate,...

Ngày tải lên: 06/03/2014, 11:20

... Florio et al superimposition of fold type I enzymes reveals that the location of the cofactor in the active site is virtually identical in all members of the group [7] Despite the high similarities ... from an integration of the sedimentation profiles shown in Fig The dissociation constants of dimer– monomer equilibria (Kd) were determined from sedimentation equilibrium experiments carried out ... apo-eSHMT is known to quench the intrinsic fluorescence emission of the enzyme [10], without changing the wavelength of maximum emission, the dissociation constant of the binding equilibrium was...

Ngày tải lên: 07/03/2014, 03:20

... for patients in E coli- chicken ———————– Drugs Ampicillin, chloramphenicol Ampicillin, gentamicin Ampicillin, spectinomycin Ampicillin, tetracycline Ampicillin, TMPϩSMX Ampicillin, gentamicin, tetracycline ... Prevalence of bacterial resistance to quinolones and other antimicrobials among avian Escherichia coli strains isolated from septicemic and healthy chickens in Spain Journal of Clinical Microbiology ... A, Ziani Z & Bouzoubaa K (1995) Antibioresistance of Escherichia coli strains isolated in Morocco from chickens with colibacillosis Veterinary Microbiology 43, 325–330 Armstrong GL, Hollingsworth...

Ngày tải lên: 08/03/2014, 09:20

... dependency of in vitro protein translocation into Escherichia coli inner-membrane vesicles varies with the signal sequence core region composition Mol Microbiol 19, 1205–1214 30 van Dalen, A., Killian, ... proteins synthesized in Escherichia coli associate with signal-recognition particle and trigger factor Mol Microbiol 25, 53–64 13 Lee, H.C & Bernstein, H.D (2001) The targeting pathway of Escherichia ... an excess of nonradioactive methionine/cysteine or by chilling on ice Subsequently, proteins were precipitated with 5% (w/v) trichloroacetic acid, followed by immunoprecipitation with a polyclonal...

Ngày tải lên: 08/03/2014, 09:20

... only a limited structural variability within the same species This prompted many investigators to attempt the isolation of antibodies directed against the conserved regions of LPS, i. e lipid A and ... spectral width of 10 p.p.m in both dimensions Prior to Fourier transformation, the FID was multiplied with a shifted sine bell window function and zero-filling was applied in F1, 1024 points The mixing ... analysis of oligosaccharide monophosphates obtained from the lipopolysaccharide of recombinant strains of Salmonella minnesota and Escherichia coli expressing the genusspecific epitope of Chlamydia...

Ngày tải lên: 08/03/2014, 09:20

... denaturation in M guanidine hydrochloride: kinetics of aggregation and reactivation Biochim Biophys Acta 1163, 89–96 18 Jaenicke R (1998) Protein self-organization in vitro and in vivo: partitioning between ... Correct folding in vitro or in vivo competes with unproductive side reactions such as misfolding or aggregation [18] Aggregation of proteins during folding, both in vitro and in vivo, is known to ... of MalZ activity (Fig 1A) and the loss of relative tryptophan fluorescence intensity (Fig 1B) with increasing concentration of urea Effect of protein concentration on refolding yield For in vitro...

Ngày tải lên: 16/03/2014, 06:20

... shown in Table 2, introduction of a H-bond donor via Asn substitution of D132 is not compatible with enzyme binding and substrate catalysis Kinetic and nucleotide-binding properties of E coli CMP ... ratio in comparison with wild-type prom tein [21] Finally, R181 in E coli CMP kinase is conserved as R149 in pig muscle AK1 Substitution of these residues by the hydrophobic side-chain of methionine ... Li de la Sierra I, Barzu O & Gilles A-M (1999) The highly similar TMP kinases of Yersinia pestis and Escherichia coli differ markedly in their AZTMP phosphorylating activity Eur J Biochem 265,...

Ngày tải lên: 16/03/2014, 10:20

... different breaking points in helix of E coli DnaG-C observed in the crystal structure (Fig 3D,E) raise questions about the flexibility of helix of E coli DnaG-C in solution Structure verification ... protein is known with this particular fold, and DnaG binds to DnaB In view of the critical importance of the C-terminal helix hairpin of DnaG-C for the interaction with DnaB [4,10], it is tempting ... New York, NY Mitkova AV, Khopde SM & Biswas SB (2003) Mechanism and stoichiometry of interaction of DnaG primase with DnaB helicase of Escherichia coli in RNA primer synthesis J Biol Chem 278,...

Ngày tải lên: 16/03/2014, 12:20

... traces in Fig 6A can be compared with the difference spectrum describing the UV-light driven formation of thymidine dimers (trace A in Fig 5) For ease of viewing, trace A of Fig is depicted again in ... Gerstenschlager I & Bacher A (1997) Biosynthesis of riboflavin: Characterization of the bifunctional deaminase-reductase of Escherichia coli and Bacillus subtilis J Bacteriol 179, 2022–2028 53 Le Grice SF ... Fig as trace A, and the time trace after 20 of white-light illumination in Fig 6A is depicted again in Fig as trace B It is obvious that a number of difference bands appear in these traces with...

Ngày tải lên: 16/03/2014, 18:20

... investigate is whether the mechanism of inducer exclusion is realized in S coelicolor Our observation that IIACrr could replace the inducer exclusion function of E coli IIAGlc by inhibition of maltose ... phosphorylation site histidine 90 of E coli IIAGlc [27,28] Overexpression and purification of S coelicolor IIACrr and IIACrr(H72A) To study the function of IIACrr, we overexpressed three crr alleles in ... and protein binding Removal of MalK by application of washing buffer revealed an RU-increase of the baseline (dotted line) indicating solely the binding of MalK to immobilized IIA protein (arrows)...

Ngày tải lên: 17/03/2014, 23:20

... polymerase I) of pBIISK (see Fig for its orientation) (b) NotI linker (5Â-AGC GGCCGCT-3Â; New England Biolabs, Ipswich, MA, USA) was ligated into the HincII site of the pBIISK polylinker (c) One linker ... and ligated into pFC13 digested with BamHI and SspI, resulting in pWVH112; and (b) inserting a linker containing the restriction sites SI, NotI and BssHII with on both ends sticky BamHI sites ... Semidry blotting, probing and visualization of (uridylylated) PII* was performed as described previously [33] Scanning of the autoradiograms and the determination of intensity of the PII* and PII*UMP...

Ngày tải lên: 23/03/2014, 04:21

... Lilley PE, Lee M, Schaeffer PM, Kralicek AV, Dixon NE & Guss JM (2006) Kinetic and crystallographic analysis of mutant Escherichia coli aminopeptidase P: C-terminal domain of E coli aminopeptidase ... cobalt(II) binding equilibrium studies of Escherichia coli methionyl aminopeptidase J Am Chem Soc 126, 12316–12324 Mitic N, Smith SJ, Neves A, Guddat LW, Gahan LR & Schenk G (2006) The catalytic ... analysis of the N-terminal domain of Escherichia coli adenylyl transferase Protein Expr Purif 34, 142–146 10 Kerr ID, Berridge G, Linton KJ, Higgins CF & Callaghan R (2003) Definition of the domain...

Ngày tải lên: 23/03/2014, 07:20

... remained obscure In this study, we characterized the aglycone-binding site of YicI by determining acceptor specificity and regioselectivity during transxylosylation Acceptor specificity and regioselectivity ... aglycone In addition, in order to explore the usefulness of the disaccharide transxylosylation products, they were tested as inhibitors of intestinal a-glucosidases Inhibitors of intestinal a-glucosidases ... Transxylosylation of YicI A M.-S Kang et al Table Initial rate of fluoride ion liberation in the presence of acceptor substrates Initial velocity was estimated by amount of released fluoride ion from...

Ngày tải lên: 23/03/2014, 07:20

... better inhibitor for digestion of CII by HflB, than CIII [11] Is this mode of antiproteolytic action of CIII a general mode of CIII activity, or does it apply only for CII? We examined the mode of ... proteolysis, with CIIIC exhibiting stronger inhibition both as a function of time (Fig 2) or as a function of concentration (Fig 3) Under the conditions of our experiment, near-total inhibition by CIIIC ... the inhibitory action of CIII on proteolysis of the heat shock sigma factor r32, another substrate of HflB Like proteolysis of CII, r32 proteolysis requires ATP Both CIII and CIIIC inhibit this...

Ngày tải lên: 23/03/2014, 10:20

... structure of holoinorganic ˚ pyrophosphatase from Escherichia coli at 1.9 A resolution Mechanism of hydrolysis Biochemistry 36, 7754–7760 Nies, D.H (1999) Microbial heavy-metal resistance Appl Microbiol ... (1999) Directed mutagenesis studies of the metal binding site at the subunit interface of Escherichia coli inorganic pyrophosphatase J Biol Chem 274, 3294–3299 Samygina, V.R., Popov, A.N., Rodina, ... had no effect on c½ Identification of the metal-binding site Fig Dependence of Escherichia coli inorganic pyrophosphatase (PPase) aggregation on cadmium acetate concentration Absorbance values...

Ngày tải lên: 23/03/2014, 13:20