Structure-guided mutagenesis of active site residues in the dengue virus two-component protease NS2B-NS3 pdf

... at the P1′ position of the dengue virus cleavage site, whereas the preferred P1′ residue of the WNV NS3 protease is G [19-21]. Theoretical molecular interactions between the active site of the ... constructed the active site mutants of the dengue virus NS3 protease, purified the recombinant proteins and assayed their enzymatic activities. MJ part...

Ngày tải lên: 10/08/2014, 05:21

... chloride in the ACE2 structure was reported in an identical position to the first bind- ing site (CL1) of tACE. Yet, in the presence of NaCl, the R169Q mutant in which the CL1 chloride-binding site ... conserved in ACE2 (His505). Indeed, the location (relative to the zinc-binding motif) of His505 in the ACE2 sequence is very similar to the location of...

Ngày tải lên: 20/02/2014, 01:20

... derivatives thereof, while maintaining the hydrophobicity of the binding site. To test the effect of the mutations on the specificity for phenylacetylated substrates, the steady-state kinetic param- eters ... k cat represents the rate of acylation and K m equals the binding constant of the substrate to the free enzyme. The results then indicate Ó FEBS 2002 Role...

Ngày tải lên: 17/03/2014, 23:20

... a medium in the catalytic reaction. The position of Lys78 is anchored with an interaction between the main chain oxygen of Ala216 and the e-amine of Lys78. In H34A mutant (Fig. 6B), the interaction between ... the reported secondary structures of pepsin-like aspartic proteases which are predominately b sheet [16]. On the basis of the kinetic behavior of the H...

Ngày tải lên: 23/03/2014, 07:20

... serine protease inhibitors. The next clear information obtained was the necessity of histidine groups which proved the active role of at least one histidine in substrate binding. The following site- directed ... PNAE is located in the middle of the biosynthetic chain starting from tryptamine and secologanin and cata- lyses the conversion of polyneuridine aldehyd...

Ngày tải lên: 24/03/2014, 04:21

... b(1,6)- linked GlcNAc moieties [5] further supporting the idea that GlcNAc-releasing activity might truly reflect the roles of these residues in DspB. Active- site residues of DspB and their role in the ... monosaccharide residues from the non- reducing end. The substrate of DspB is a hexosamine- containing matrix polysaccharide consisting of a linear homoglycan...

Ngày tải lên: 16/03/2014, 06:20

... either of the region involved in DNase I general sensitivity or of the region for the formation of DHSs, although chromatin opening activity of the LCR has been des- cribed in several loci using ... November 2004) doi:10.1111/j.1742-4658.2004.04482.x The role of DNase I hypersensitive sites (DHSs) in transcription of the B cell-specific Ig-b gene and in maintena...

Ngày tải lên: 19/02/2014, 16:20

... carbonyl of Asn by the nitrogen of the peptide group linking the Asn to the following residue [4–6] 2 . As hydrolysis of this intermediate may occur on either side of the imide nitrogen, the Asp ... min,  36 min and  39 min. In addition, several minor over- lapping peaks were also observed, thus proving that the presence of one or more isoAsp residues in the...

Ngày tải lên: 08/03/2014, 08:20

... test the importance of the conserved Glu residues in the EX 7 E motif of these proteins by the stringent criterion of their ability to support cell growth. Our results indicate that both Glu residues ... importance in vivo of conserved glutamate residues in the EX 7 E motif retaining glycosyltransferase Gpi3p, the UDP-GlcNAc-binding subunit of the first enzy...

Ngày tải lên: 30/03/2014, 20:20

... the sentences to be marked-up. Ac tive learning is realized as query 16 Chapter 7 Monitoring, assessing and terminating the learning process The monitoring, assessing and terminating of the active ... Eric Ringger, Kevin Seppi, James Carroll and Peter Mc- Clanahan 2008. As se ss ing the costs of sampling methods in active learn- ing for annotation. Proceedings of the 4...

Ngày tải lên: 02/06/2014, 09:22