rna isolation and characterization protocols

... Molecular Biology, Vol 86 RNA Isolation and Characterizabon Protocols Ed&d by R Rapley and D L Manmng 0 Humana Press Inc , Totowa, NJ 27 28 Cheng and Seemann To isolate RNA, plant tissue IS ... Molecular 81o/ogy, Vol 86 RNA Isolation and Charactematron Protocols E&ted by R Rapley and 0 L Mannmg 0 Humana Press Inc , Totowa, NJ 75 16 Batt, Carmichael, an...

Ngày tải lên: 11/04/2014, 10:30

... Journal of Water and Environment Technology, Vol.3, No.2, 2005 - 289 - Isolation and characterization of an extracellular polymer closely ... and Kadowaki, K. (1985a) Flocculant production by Paecilomyces sp. Taxonomic studies and culture conditions for production. Agric. Biol. Chem., Vol.49, No.11, 3151-3157. Journal of Water and ... polysaccharides, although proteins an...

Ngày tải lên: 05/09/2013, 09:08

... North-Holland, Amsterdam. P. V. Castilho et al. Characterization of four pulchellin isoforms FEBS Journal 275 (2008) 948–959 ª 2008 The Authors Journal compilation ª 2008 FEBS 959 Isolation and characterization ... inhibitory concentration and median lethal dose values indicate that P I and P II have similar toxicities and that both are more toxic to cultured HeLa cells and mi...

Ngày tải lên: 18/02/2014, 16:20

... curves. Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5. Triangles and continuous line: data and fitting with PHI/PHO ratio of 1. Asterisks and broken line: data and fitting with ... Avanzate, Universita ` del Piemonte Orientale, Alessandria, Italy; 3 Dipartimento di Biochimica e Biologia Molecolare, Universita ` di Parma, Italy This paper reports the isolation...

Ngày tải lên: 21/02/2014, 00:20

... York, USA. Ó FEBS 2003 Characterization of ccNiR subunits (Eur. J. Biochem. 270) 3915 The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio ... ¼ 1/2) with g max values at 3.6, 3.5, 3.2, 3.0 and 2.96 [23,24]. In this communication, we report for the first time the isolation and biochemical characterization of D. desul...

Ngày tải lên: 21/02/2014, 00:20

... were used to sequence the Ch-Prx1 cDNA and t he Ch-Prx1 gene, respectiv ely. RNA isolations RNA for RT-PCR and for northern a nalyses was islolated by alternative m ethods. I n t he ®rst method, ... ctivity, and its ability t o u se different thioredoxin isoforms as hydrogen donors. To better understand the function of Ch-Prx1 in vivo, we isolated the cDNA and the Ch-Prx1 gene, a...

Ngày tải lên: 08/03/2014, 16:20

... higher d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation. Results In silico characterization and isolation of the ... Journal 272 (2005) 1291–1304 ª 2005 FEBS Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana Anja Riemenschneider, Rosalina Wegele, Ahl...

Ngày tải lên: 16/03/2014, 18:20

... respect- ively, and the resultant V NAR s evaluated for retention of binding affinity. Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... CSIRO (Health Sciences and Nutrition) animal ethics requirements. Total RNA was extracted using the AquaPure RNA Isolation Kit (BIO-RAD, Australia), stored at )80 °C, a...

Ngày tải lên: 17/03/2014, 10:20

... Temperature-dependent ricin A chain mutants FEBS Journal 274 (2007) 5586–5599 ª 2007 The Authors Journal compilation ª 2007 FEBS 5599 The isolation and characterization of temperature-dependent ricin ... FEBS 5591 and smaller intact rRNA species. The released frag- ments were quantified relative to 5.8S rRNA to control for differences in gel loading, and the percentage of dep- urinat...

Ngày tải lên: 23/03/2014, 07:20

... human and bovine tissues and cell types. MUC15 mRNA expression was examined by PCR screening of commercial multiple tissue cDNA panels and oligo(dT) primed cDNA libraries and by RT-PCR on RNA isolated ... Oeynhausen, Germany) and sequenced as described for the bovine counterpart. Detection of an alternatively spliced MUC15 variant First-strand cDNA was prepared from the mammary gl...

Ngày tải lên: 24/03/2014, 04:21