mouse and human mesoangioblasts isolation and characterization from adult skeletal muscles
Báo cáo khoa học: Death-associated protein kinase (DAPK) and signal transduction: fine-tuning of autophagy in Caenorhabditis elegans homeostasis doc
... Interestingly, DAPK-1 and MAP1B colocalize with a-tubulin and F-actin [61] Because autophagosomes slide along cytoskeletal structures and fuse with lysosomes [62], DAPK-1 and MAP1B located to ... signaling induces excessive autophagy and causes the death of worms during starvation, and that excessive autophagy causes defects in the pharyngeal muscles and eventually contributes to Fine-tuning ... release Beclin from Bcl-2-dependent inhibition and thereby activate autophagy, (e) extracellular signal-regulated kinase, which phosphorylates and activates Ga interacting proteins and stimulates...
- 8
- 376
- 0
Báo cáo sinh học: "Regulation of metabolism in Caenorhabditis elegans longevity" ppsx
... specific to C elegans and other invertebrates The soma of the adult nematode is postmitotic, and metabolic control might have different Page of effects in C elegans (where adult cell and tissue replace ... - and to regulate transcription and protein biogenesis and degradation accordingly TOR exists in two highly conserved protein complexes: TORC1, which regulates cell growth, protein synthesis and ... and autophagy; and TORC2, which regulates cytoskeletal reorganization [12] Both complexes regulate the meta bolic state of C elegans TOR activates the ribosomal p70 S6 kinase (S6K) and the translation...
- 3
- 389
- 0
Báo cáo sinh học: "Robust and specific inhibition of microRNAs in Caenorhabditis elegans" doc
... to study the biological role of small non-coding RNAs and yet, to date, no standard techniques or protocols are available to effectively and conveniently knockdown miRNA function transiently ... bind to and deplete the available pool of target miRNA, thus inhibiting miRNA function in the animal Page of Zheng and colleagues [2] used antisense oligonucleotides complementary to lin-4 and let-7 ... report by Zheng and colleagues [2] convincingly suggest that this new class of antisense oligonucleotides could prove to be a very powerful and effective tool for the robust and specific inhibition...
- 3
- 204
- 0
Báo cáo khoa học: Functional analysis of pyrimidine biosynthesis enzymes using the anticancer drug 5-fluorouracil in Caenorhabditis elegans docx
... elegans TP and UP participate in both uridine and thymidine synthesis, and humans possess at least two different UPs and one TP This complex redundancy makes the relationship between UP ⁄ TP and 5-FU ... wild-type worms and three upp-1 mutants Fig S3 Sequence alignments of C elegans UPP-1 and human UPP-1 Fig S4 Sequence alignments of C29F7.3 and F40F8.1 Fig S5 Sequence alignments of human UMPS and worm ... with amino acid homology to human UP (UPP-1 and UPP-2) (46% identical; Fig S3) In order to verify the functional conservation between human and worm UPP-1, we expressed human UPP-1 under the control...
- 12
- 316
- 0
Báo cáo khoa học: Stage-specific activation of MIG-17⁄ADAMTS controls cell migration in Caenorhabditis elegans docx
... stages using Monoclonal 2, we detected strong bands corresponding to the activated form during L3 and L4, weak bands in L2 and the adult stage, and no bands in L1 (Fig 3D) These results indicated ... baculovirus and was efficiently secreted into the culture medium MIG-17–6His was purified from the culture medium using an Ni2+-chelating Sepharose FF column, and two protein bands of 70 and 43 kDa, ... activation of MIG-17 ⁄ ADAMTS S Ihara and K Nishiwaki A B C D E F Fig MIG-17 activation in the adult stage Cross-sections of young adults (A–C) and 1-day-old adults (D, E) expressing MIG-17–GFP...
- 10
- 341
- 0
Báo cáo sinh học: "A global analysis of genetic interactions in Caenorhabditis elegans" doc
... calculated as a standard Z-score (l - m)/s where l is the observed number of links in the subnetwork, and m and s are the mean and standard deviation of the number of links across 1,000 random collections ... query network reveals novel interactions between bar-1 and glp-1, between bar-1 and sma-6, and between bar-1 and multiple components of the FGF and EGF pathways Further investigation will be required ... interactions from S cerevisiae, Drosophila melanogaster, and human protein interactions contained in BioGRID [41], an mRNA coexpression network constructed from C elegans, S cerevisiae, D melanogaster, and...
- 27
- 393
- 0
Báo cáo y học: "Identification of ciliated sensory neuron-expressed genes in Caenorhabditis elegans using targeted pull-down of poly(A) tails" ppsx
... cells RNA purified from che-2::PABP was labeled with Cy5 and that from acr-5::PABP was labeled with Cy3 The two types of labeled RNA were mixed and hybridized to the cDNA microarray and the che2::PABP/acr-5::PABP ... RNA from both che-2::PABP and acr-5::PABP The mRNA for unc-54, which is expressed in muscles [23], was enriched in RNA from myo-3::PABP Representatives of housekeeping genes, eft-3 [27] and lmn-1 ... point were different from the product amplified from the cDNA In Figure 2, eft-3 was amplified for 25 cycles, lmn-1, snt-1 and unc-54 for 30 cycles and tax-2, unc-8, odr10, del-1 and acr-5 for 35...
- 13
- 301
- 0
Báo cáo y học: "Combinatorial RNA interference in Caenorhabditis elegans reveals that redundancy between gene duplicates can be maintained for more than 80 million years of evolution" pdf
... sterility, and growth defects using a dissecting microscope To score post-embryonic phenotypes (Table and Table 3), L1 larvae from the 96-well liquid feeding assay were collected after 96 hours and ... elegans were duplicated before the divergence from the related nematode C briggsae (see Materials and methods, below; Additional data file 4) C elegans and C briggsae, despite being morphologically ... redundancy [12,13], and indirect experimental evidence for the redundant functions of duplicated genes comes from the analysis of lossof-function phenotypes of single genes; in both yeast and worms, inactivation...
- 13
- 318
- 0
Báo cáo y học: "Identification of ciliary and ciliopathy genes in Caenorhabditis elegans through comparative genomics" pptx
... from NHGRI, NSERC and a start-up fund from Simon Fraser University DLB is supported by grants from NSERC, CIHR of Canada and from Genome Canada and Genome British Columbia DGM and MAM are supported ... Genome Canada and Genome British Columbia MRL is supported by a grant from the March of Dimes and holds scholar awards from CIHR and MSFHR OEB was supported by a MSFHR fellowship and is currently ... worms Eggs were prepared from gravid adults using a hypochlorite treatment [59], resuspended in 10 mM Tris-EDTA (pH 7.5) and stored at -80°C RNA isolation, analysis and labeling Thawed embryos...
- 12
- 326
- 0
Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt
... spontaneous and EFSevoked release of ATP in the bladder are mediated differentially by CD38 Results Mechanisms of spontaneous and EFS-evoked release of ATP in bladder detrusor muscles from CD38+ ⁄ + and ... from CD38+ ⁄ + and CD38) ⁄ ) mice (C) Inset: western immunoblot analysis of SNAP-25 shows a single band at 25 kDa in homogenates from control (vehicle-treated) tissues An additional 24-kDa band ... of ATP in bladder detrusor muscles isolated from CD38+ ⁄ + and CD38) ⁄ ) mice: the amounts of ATP were 0.86 ± 0.14 and 0.70 ± 0.13 fmolÆmg)1 tissue in the absence and presence of carbachol, respectively...
- 14
- 509
- 0
Báo cáo Y học: Dystrobrevin requires a dystrophin-binding domain to function in Caenorhabditis elegans doc
... and various restriction enzyme sites were removed and added by performing synonymous point mutations to yield the construct dyb-1:gfp VII, which has single AgeI and MluI sites at codons 390 and ... construct AN450 and the deletion derivatives described above were transferred from pGEX into dyb-1:gfp VII using PCR-amplifying procedures with primers carrying AgeI and MluI sites, and cloned into ... R Bourette, C Bourgin and V Corset for ´ ´ helpful discussions, and A Dumont, P Merel, and S Quetat for their participation in this work during their training courses K G and L S were supported...
- 6
- 334
- 0
Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
... B=MICB Þ=n where A and B are the MICs of drug A and drug B in the combination, MICA and MICB are the MICs of drug A and drug B alone, FICA and FICB are the FICs of drug A and drug B and n is the number ... PR-39, from pig intestine [10]; (b) derivatives of pleurocidin, a fish-derived antimicrobial peptide, and dermaseptin, from frog skin [11]; (c) drosocin and pyrrhocoricin, from insects [12]; and ... (Gal-ONp) calcein and LPS from E coli serotype O111:B4 were all purchased from Sigma (St Louis, MO, USA) SYTOX Green was from Molecular Probes (Invitrogen, Carlsbad, CA, USA) Egg yolk PG and PE were...
- 18
- 494
- 0
Báo cáo khoa học: Lysosomal localization of GLUT8 in the testis – the EXXXLL motif of GLUT8 is sufficient for its intracellular sorting via AP1- and AP2-mediated interaction docx
... monoclonal mouse antihaemagglutinin tag (Covance Research Products Inc., Berkeley, CA, USA), anti -mouse Golgi marker GM130 and anti -mouse LAMP1 (1D4B), mouse anti-AP1c, -AP2a, -AP3d and -AP4e, mouse ... Germany), mouse monoclonal anti -human LAMP1 (H4A3), mouse monoclonal anti -human LAMP2 (H4B4), and rat anti -mouse LAMP2 (ABL-93) (Developmental Studies Hybridoma Bank at the University of Iowa) The mouse ... ⁄ and AP2 ⁄ GLUT8 NICD interactions (Fig 2C,D) This residual association with AP1 and AP2 might be due to high and variable concentrations of AP1 and AP2 in these extracts or could result from...
- 15
- 508
- 0
Báo cáo khoa học: Tumour necrosis factor-a attenuates insulin action on phosphoenolpyruvate carboxykinase gene expression and gluconeogenesis by altering the cellular localization of Foxa2 in HepG2 cells pptx
... with TBP and b-actin antibodies and taken as nuclear and cytosolic loading controls, respectively, and also used to ascertain the purity of the nuclear and cytosolic preparations (A) Bands were ... Materials and methods Materials DMEM, antibiotic–antimycotic, protein A-Sepharose, human insulin and TNFa were purchased from Sigma (St Louis, MO, USA) The glucose assay, protein estimation and RNeasy ... secondary antibodies used were obtained from Bangalore Genei, India All other chemicals and reagents used were purchased from Sigma Control and Foxa2 siRNA was obtained from Santa Cruz Biotechnology Inc...
- 13
- 449
- 0
Báo cáo khoa học: Glutathione transferases kappa 1 and kappa 2 localize in peroxisomes and mitochondria, respectively, and are involved in lipid metabolism and respiration in Caenorhabditis elegans pot
... observed in the body wall muscles (Fig 2C) and in the rectal gland cells in larvae and adults (Fig 2D) The presence of a peroxisomal targeting signal in its C-terminus (Fig 1) and the punctate localization ... interface GSTK-1 and GSTK-2 are localized in peroxisomes and mitochondria, respectively The analysis of GST kappa transcript levels has shown a ubiquitous expression in human [4] and mouse [6] tissues ... stomach and heart, and its association with liver and kidney mitochondria has been demonstrated by electron microscopy [6] GSTK1 transcript tissue expression is similar in the rat and in the mouse...
- 11
- 380
- 0
Báo cáo khoa học: The astacin protein family in Caenorhabditis elegans docx
... representing a mixed population of adults, all four larval stages and eggs, were harvested and separated from bacteria as described elsewhere [20] RNA purification For the isolation of RNA, 100 lg fresh ... DNA was isolated from mL fresh nematodes from a liquid culture using a standard protocol [22] PCR amplification and cloning Polyadenylated RNA (1 lg) was converted into singlestranded cDNA using ... Kent and Zahler [31] (Table and http://www.zoo.uni-heidelberg.de/moehrlen/docs/ WebFig2.htm) For NAS-1, NAS-21, NAS-22 and NAS28 we observed aberrant splice sites from both, the Genefinder and...
- 12
- 387
- 0
Báo cáo khoa học: Identification and localization of glycine in the inner core lipopolysaccharide of Neisseria meningitidis ppt
... 269) 4171 Table Negative ion ES-MS data and proposed compositions of core oligosaccharide from N meningitidis strains L3 galE, L4 galE, NGH15 B5– and B5+ and BZ157 galE B5+ Average mass units were ... glycine, species with and without an O-acetyl group and the PEtn residue were also observed (Table 1) As indicated in Table for L4 galE core oligosaccharide, the ions at m/z 969 and 987 correspond ... were observed and the compositions for each glycoform are listed in Table Typical ions differing by 18 a.m.u were observed for each glycoform and corresponded to reducing end anhydro and intact...
- 7
- 449
- 1
A pathway that links reproductive status to lifespan in Caenorhabditis elegans pptx
... mentioned above, forcing the germline stem cells to exit mitosis and enter meiosis during adulthood extends the lifespan of both adult flies and adult worms.12,14 Moreover, in flies, as in worms, this treatment ... intestinal nuclei during adulthood Acts in the intestine to extend lifespan daf-12 Nuclear hormone receptor daf-9 Cytochrome P450 required for DAF-12-ligand biosynthesis Ortholog of the human transcription ... the story has begun to emerge (and is summarized in Figs and 3) First, the pathway that triggers DAF-16/FOXO nuclear localization is at least partially distinct from the pathway that localizes...
- 7
- 446
- 0
Báo cáo sinh học: "The short coiled-coil domain-containing protein UNC-69 cooperates with UNC-76 to regulate axonal outgrowth and normal presynaptic organization in Caenorhabditis elegans" ppt
... existing cDNA clones and subsequently subcloned into pRE192 and pRH143 to create LexA-bait and GAD-prey plasmids pRE192 and pRH143 are derivatives of pBTM116 and pGAD424, respectively, and are gifts ... 2006, Volume 5, Article Su and Tharin et al 9.13 in Figure 7d) and lies within a region conserved from worms to humans (gray-shaded region in Figure 7d) UNC-69-binding site, and UNC-76 may require ... the processes and growth cones of developing neurites (arrowhead in Figure 4c) In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular...
- 26
- 399
- 0
Báo cáo khoa học: "Translocational changes of localization of synapsin in axonal sprouts of regenerating rat sciatic nerves after ligation crush injury" pptx
... 3B) IR was extended from the node of Ranvier (Fig 3C) IR was observed in the axolemma and throughout the axoplasm IR was exhibited to both proximal and distal directions from the node in the ... at days (Fig 11), 14 days, and 28 days after release from the ligation But after days of release, the Significant amounts of proteins and materials are transported from the site of their synthesis ... ligation crush injury and the involvement of SyI in vesicular transport of membrane elongation in the sciatic nerve regeneration Materials and Methods Experimental animals Forty-eight adult male Sprague-Dawley...
- 9
- 251
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25