mouse and human mesoangioblasts isolation and characterization from adult skeletal muscles

... Interestingly, DAPK-1 and MAP1B colocalize with a-tubulin and F-actin [61] Because autophagosomes slide along cytoskeletal structures and fuse with lysosomes [62], DAPK-1 and MAP1B located to ... signaling induces excessive autophagy and causes the death of worms during starvation, and that excessive autophagy causes defects in the pharyngeal muscles and eventually contributes to Fine-tuning ... release Beclin from Bcl-2-dependent inhibition and thereby activate autophagy, (e) extracellular signal-regulated kinase, which phosphorylates and activates Ga interacting proteins and stimulates...

Ngày tải lên: 29/03/2014, 08:20

... specific to C elegans and other invertebrates The soma of the adult nematode is postmitotic, and metabolic control might have different Page of effects in C elegans (where adult cell and tissue replace­ ... - and to regulate transcription and protein biogenesis and degradation accordingly TOR exists in two highly conserved protein complexes: TORC1, which regulates cell growth, protein synthesis and ... and autophagy; and TORC2, which regulates cytoskeletal reorganization [12] Both complexes regulate the meta­ bolic state of C elegans TOR activates the ribosomal p70 S6 kinase (S6K) and the translation...

Ngày tải lên: 06/08/2014, 19:21

... to study the biological role of small non-coding RNAs and yet, to date, no standard techniques or protocols are available to effectively and conveniently knockdown miRNA function transiently ... bind to and deplete the available pool of target miRNA, thus inhibiting miRNA function in the animal Page of Zheng and colleagues [2] used antisense oligonucleotides complementary to lin-4 and let-7 ... report by Zheng and colleagues [2] convincingly suggest that this new class of antisense oligonucleotides could prove to be a very powerful and effective tool for the robust and specific inhibition...

Ngày tải lên: 06/08/2014, 19:21

... elegans TP and UP participate in both uridine and thymidine synthesis, and humans possess at least two different UPs and one TP This complex redundancy makes the relationship between UP ⁄ TP and 5-FU ... wild-type worms and three upp-1 mutants Fig S3 Sequence alignments of C elegans UPP-1 and human UPP-1 Fig S4 Sequence alignments of C29F7.3 and F40F8.1 Fig S5 Sequence alignments of human UMPS and worm ... with amino acid homology to human UP (UPP-1 and UPP-2) (46% identical; Fig S3) In order to verify the functional conservation between human and worm UPP-1, we expressed human UPP-1 under the control...

Ngày tải lên: 30/03/2014, 01:20

... stages using Monoclonal 2, we detected strong bands corresponding to the activated form during L3 and L4, weak bands in L2 and the adult stage, and no bands in L1 (Fig 3D) These results indicated ... baculovirus and was efficiently secreted into the culture medium MIG-17–6His was purified from the culture medium using an Ni2+-chelating Sepharose FF column, and two protein bands of 70 and 43 kDa, ... activation of MIG-17 ⁄ ADAMTS S Ihara and K Nishiwaki A B C D E F Fig MIG-17 activation in the adult stage Cross-sections of young adults (A–C) and 1-day-old adults (D, E) expressing MIG-17–GFP...

Ngày tải lên: 30/03/2014, 04:20

... calculated as a standard Z-score (l - m)/s where l is the observed number of links in the subnetwork, and m and s are the mean and standard deviation of the number of links across 1,000 random collections ... query network reveals novel interactions between bar-1 and glp-1, between bar-1 and sma-6, and between bar-1 and multiple components of the FGF and EGF pathways Further investigation will be required ... interactions from S cerevisiae, Drosophila melanogaster, and human protein interactions contained in BioGRID [41], an mRNA coexpression network constructed from C elegans, S cerevisiae, D melanogaster, and...

Ngày tải lên: 06/08/2014, 18:21

... cells RNA purified from che-2::PABP was labeled with Cy5 and that from acr-5::PABP was labeled with Cy3 The two types of labeled RNA were mixed and hybridized to the cDNA microarray and the che2::PABP/acr-5::PABP ... RNA from both che-2::PABP and acr-5::PABP The mRNA for unc-54, which is expressed in muscles [23], was enriched in RNA from myo-3::PABP Representatives of housekeeping genes, eft-3 [27] and lmn-1 ... point were different from the product amplified from the cDNA In Figure 2, eft-3 was amplified for 25 cycles, lmn-1, snt-1 and unc-54 for 30 cycles and tax-2, unc-8, odr10, del-1 and acr-5 for 35...

Ngày tải lên: 14/08/2014, 14:21

... sterility, and growth defects using a dissecting microscope To score post-embryonic phenotypes (Table and Table 3), L1 larvae from the 96-well liquid feeding assay were collected after 96 hours and ... elegans were duplicated before the divergence from the related nematode C briggsae (see Materials and methods, below; Additional data file 4) C elegans and C briggsae, despite being morphologically ... redundancy [12,13], and indirect experimental evidence for the redundant functions of duplicated genes comes from the analysis of lossof-function phenotypes of single genes; in both yeast and worms, inactivation...

Ngày tải lên: 14/08/2014, 17:22

... from NHGRI, NSERC and a start-up fund from Simon Fraser University DLB is supported by grants from NSERC, CIHR of Canada and from Genome Canada and Genome British Columbia DGM and MAM are supported ... Genome Canada and Genome British Columbia MRL is supported by a grant from the March of Dimes and holds scholar awards from CIHR and MSFHR OEB was supported by a MSFHR fellowship and is currently ... worms Eggs were prepared from gravid adults using a hypochlorite treatment [59], resuspended in 10 mM Tris-EDTA (pH 7.5) and stored at -80°C RNA isolation, analysis and labeling Thawed embryos...

Ngày tải lên: 14/08/2014, 17:22

... spontaneous and EFSevoked release of ATP in the bladder are mediated differentially by CD38 Results Mechanisms of spontaneous and EFS-evoked release of ATP in bladder detrusor muscles from CD38+ ⁄ + and ... from CD38+ ⁄ + and CD38) ⁄ ) mice (C) Inset: western immunoblot analysis of SNAP-25 shows a single band at 25 kDa in homogenates from control (vehicle-treated) tissues An additional 24-kDa band ... of ATP in bladder detrusor muscles isolated from CD38+ ⁄ + and CD38) ⁄ ) mice: the amounts of ATP were 0.86 ± 0.14 and 0.70 ± 0.13 fmolÆmg)1 tissue in the absence and presence of carbachol, respectively...

Ngày tải lên: 05/03/2014, 23:20

... and various restriction enzyme sites were removed and added by performing synonymous point mutations to yield the construct dyb-1:gfp VII, which has single AgeI and MluI sites at codons 390 and ... construct AN450 and the deletion derivatives described above were transferred from pGEX into dyb-1:gfp VII using PCR-amplifying procedures with primers carrying AgeI and MluI sites, and cloned into ... R Bourette, C Bourgin and V Corset for ´ ´ helpful discussions, and A Dumont, P Merel, and S Quetat for their participation in this work during their training courses K G and L S were supported...

Ngày tải lên: 08/03/2014, 23:20

... B=MICB Þ=n where A and B are the MICs of drug A and drug B in the combination, MICA and MICB are the MICs of drug A and drug B alone, FICA and FICB are the FICs of drug A and drug B and n is the number ... PR-39, from pig intestine [10]; (b) derivatives of pleurocidin, a fish-derived antimicrobial peptide, and dermaseptin, from frog skin [11]; (c) drosocin and pyrrhocoricin, from insects [12]; and ... (Gal-ONp) calcein and LPS from E coli serotype O111:B4 were all purchased from Sigma (St Louis, MO, USA) SYTOX Green was from Molecular Probes (Invitrogen, Carlsbad, CA, USA) Egg yolk PG and PE were...

Ngày tải lên: 16/03/2014, 00:20

... monoclonal mouse antihaemagglutinin tag (Covance Research Products Inc., Berkeley, CA, USA), anti -mouse Golgi marker GM130 and anti -mouse LAMP1 (1D4B), mouse anti-AP1c, -AP2a, -AP3d and -AP4e, mouse ... Germany), mouse monoclonal anti -human LAMP1 (H4A3), mouse monoclonal anti -human LAMP2 (H4B4), and rat anti -mouse LAMP2 (ABL-93) (Developmental Studies Hybridoma Bank at the University of Iowa) The mouse ... ⁄ and AP2 ⁄ GLUT8 NICD interactions (Fig 2C,D) This residual association with AP1 and AP2 might be due to high and variable concentrations of AP1 and AP2 in these extracts or could result from...

Ngày tải lên: 16/03/2014, 02:20

... with TBP and b-actin antibodies and taken as nuclear and cytosolic loading controls, respectively, and also used to ascertain the purity of the nuclear and cytosolic preparations (A) Bands were ... Materials and methods Materials DMEM, antibiotic–antimycotic, protein A-Sepharose, human insulin and TNFa were purchased from Sigma (St Louis, MO, USA) The glucose assay, protein estimation and RNeasy ... secondary antibodies used were obtained from Bangalore Genei, India All other chemicals and reagents used were purchased from Sigma Control and Foxa2 siRNA was obtained from Santa Cruz Biotechnology Inc...

Ngày tải lên: 16/03/2014, 02:20

... observed in the body wall muscles (Fig 2C) and in the rectal gland cells in larvae and adults (Fig 2D) The presence of a peroxisomal targeting signal in its C-terminus (Fig 1) and the punctate localization ... interface GSTK-1 and GSTK-2 are localized in peroxisomes and mitochondria, respectively The analysis of GST kappa transcript levels has shown a ubiquitous expression in human [4] and mouse [6] tissues ... stomach and heart, and its association with liver and kidney mitochondria has been demonstrated by electron microscopy [6] GSTK1 transcript tissue expression is similar in the rat and in the mouse...

Ngày tải lên: 16/03/2014, 03:20

... representing a mixed population of adults, all four larval stages and eggs, were harvested and separated from bacteria as described elsewhere [20] RNA purification For the isolation of RNA, 100 lg fresh ... DNA was isolated from mL fresh nematodes from a liquid culture using a standard protocol [22] PCR amplification and cloning Polyadenylated RNA (1 lg) was converted into singlestranded cDNA using ... Kent and Zahler [31] (Table and http://www.zoo.uni-heidelberg.de/moehrlen/docs/ WebFig2.htm) For NAS-1, NAS-21, NAS-22 and NAS28 we observed aberrant splice sites from both, the Genefinder and...

Ngày tải lên: 23/03/2014, 15:21

... 269) 4171 Table Negative ion ES-MS data and proposed compositions of core oligosaccharide from N meningitidis strains L3 galE, L4 galE, NGH15 B5– and B5+ and BZ157 galE B5+ Average mass units were ... glycine, species with and without an O-acetyl group and the PEtn residue were also observed (Table 1) As indicated in Table for L4 galE core oligosaccharide, the ions at m/z 969 and 987 correspond ... were observed and the compositions for each glycoform are listed in Table Typical ions differing by 18 a.m.u were observed for each glycoform and corresponded to reducing end anhydro and intact...

Ngày tải lên: 23/03/2014, 21:21

... mentioned above, forcing the germline stem cells to exit mitosis and enter meiosis during adulthood extends the lifespan of both adult flies and adult worms.12,14 Moreover, in flies, as in worms, this treatment ... intestinal nuclei during adulthood Acts in the intestine to extend lifespan daf-12 Nuclear hormone receptor daf-9 Cytochrome P450 required for DAF-12-ligand biosynthesis Ortholog of the human transcription ... the story has begun to emerge (and is summarized in Figs and 3) First, the pathway that triggers DAF-16/FOXO nuclear localization is at least partially distinct from the pathway that localizes...

Ngày tải lên: 28/03/2014, 16:20

... existing cDNA clones and subsequently subcloned into pRE192 and pRH143 to create LexA-bait and GAD-prey plasmids pRE192 and pRH143 are derivatives of pBTM116 and pGAD424, respectively, and are gifts ... 2006, Volume 5, Article Su and Tharin et al 9.13 in Figure 7d) and lies within a region conserved from worms to humans (gray-shaded region in Figure 7d) UNC-69-binding site, and UNC-76 may require ... the processes and growth cones of developing neurites (arrowhead in Figure 4c) In older larvae and adults, UNC-69::GFP was expressed in neurons of the anterior, lateral, ventral and retro-vesicular...

Ngày tải lên: 06/08/2014, 18:21