vascular smooth muscle cells isolation culture and characterization

... concentrations of LPS and IFN NO production is reported to be stimulated by LPS and IFN through independent signal pathways; LPS and IFN stimulate cells by activating NF-jB and the interferon regulatory ... 17 Jeremy, J.Y., Rowe, D., Emsley, A.M & Newby, A.C (1999) Nitric oxide and the proliferation of vascular smooth muscle cells Cardiovasc Res 43, 580–594 2372 K Kogure et al (Eur J Biochem 269) ... Sakashita, M & Tanaka, S (1994) Lysophosphatidic acids induce proliferation of cultured vascular smooth muscle cells from rat aorta Am J Physiol 267, C204–C210 27 Green, L.C., Wagner, D.A., Glogowski,...

Ngày tải lên: 22/02/2014, 04:20

... of JAK2 in vascular smooth muscle cells is dependent on PLD2 Diabetes 52, 2381–2388 Kim S & Iwao H (2000) Molecular and cellular mechanisms of angiotensin II-mediated cardiovascular and renal ... procedures Cell culture Rat VSMCs were isolated and subcultured as described previously [34] Cells used in the experiments were from passages 3–5 VSMCs were allowed to attach to the plate wall and were ... thrombin-induced vascular smooth muscle cell motility J Biol Chem 280, 3112–3120 Han M, Wen JK, Zheng B, Cheng Y & Zhang C (2006) Serum deprivation results in redifferentiation of human umbilical vascular smooth...

Ngày tải lên: 30/03/2014, 02:20

... neurons and smooth muscle cells [30–33] In addition, Ab peptides with the Dutch E22Q and Iowa D23N mutations have been shown to be toxic to human leptomeningeal smooth muscle cells in culture ... plating VSMC cultured on Ab1–40 and Ab1–42 released significantly more lactate dehydrogenase into the medium than cells cultured on plastic alone (Fig 1, P < 0.05 and P < 0.005 for Ab1–40 and Ab1–42, ... shows that substratebound Ab can increase apoptotic cell death in vascular smooth muscle cell cultures in vitro and that the cardiovascular risk factor homocysteine increases Ab-induced cell death...

Ngày tải lên: 22/02/2014, 07:20

... half way between the A1234 cells and the Page of 12 control cells Apparently both MAPK and PAK contribute to the motility of A7r5 cells and these effects are comparable and additive Effect of phosphomimetic ... untransfected cells The relative migration activity for the A1234-transfected cells was about 13% of the control cells (EGFP), and the ratios of A1A2 and A3A4 were 49% and 47% of the control cells, ... for cell motility, and that MAPK and PAK work independently and additively toward this process Since only l-CaD is expressed in these cultured cells, CaD refers to the non -muscle isoform exclusively...

Ngày tải lên: 10/08/2014, 05:21

... airway smooth muscle (HASM) isolated from the trachealis muscle and propagated as described previously [9,10] were used in this study The cells were plated at a density of 1.0 × 104 cells/ cm2 and ... in airway smooth muscle cells [28,29] Recent investigations from our laboratory have shown that the inflammatory cytokines increase the expression of CD38 in human airway smooth muscle cells [5,7,8] ... growth-arrested HASM cells at confluence The media were aspirated and washed with ice-cold PBS containing phosphatase inhibitors and the cells were scraped in ml of the same buffer The cells were pelletted...

Ngày tải lên: 12/08/2014, 15:21

... with cogans’s syndrome: deleterious loss of vascular structural integrity is associated with GM-CSF overstimulation in macrophages and smooth muscle cells Journal of Cardiothoracic Surgery 2010 ... areas collocated with clusters of inflammatory cells (Fig 3B and 3C) MMP1 (Fig 3D) and MMP9 (Fig 3E) were strongly expressed at the intima-media border and in deeper areas of the media Interestingly, ... infiltrating cells particularly at the intima-media border (arrows indicate areas with profound vascularization) MMP1 (panel D) and MMP9 (panel E) were strongly expressed at the intima-media border and...

Ngày tải lên: 10/08/2014, 09:22

... phenotypically characterized using muscle- specific antibodies against smooth muscle actin and myosin Cells were maintained in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS) and antibiotics in a humidified ... variations in loading and transfer mRNA stability assay Cells were cultured in tissue culture flasks as described above and either preincubated or not with pharmacological inhibitors and further treated ... transfection and coexpression experiments Cultured cells were plated at a density of · 105 cm)2 in 60-mm tissue culture dishes and maintained in medium containing 10% serum for 18 h Cells were...

Ngày tải lên: 19/02/2014, 16:20

... vascular smooth muscle cells through modulation of various steps of growth signals Lipids 31 (Suppl ), S301–S304 16 Pakala, R., Sheng, W.L & Benedict, C.R (2000) Vascular smooth muscle cells preloaded ... Koumanov, K., Bereziat, G & Andreani, M (1999) Interleukin 1beta induces type II-secreted phospholipase A(2) gene in vascular smooth muscle cells by a nuclear factor kappaB and peroxisome proliferatoractivated ... Expression of caveolin-3 in skeletal, cardiac, and smooth muscle cells Caveolin-3 is a component of the sarcolemma and co-fractionates with dystrophin and dystrophin-associated glycoproteins J Biol...

Ngày tải lên: 19/02/2014, 16:20

... Accumulation of cerebral vascular fibrillar Aβ has been shown to cause marked degeneration and cell death of smooth muscle cells and pericytes in affected larger cerebral vessels and in cerebral microvessels, ... including reactive astrocytes and activated microglia, as well as inflammatory cytokines and chemokines [20,21] Additionally, cerebral vascular smooth muscle cells and pericytes, which degenerate ... proliferating and degenerating smooth muscle cells Amyloid 1994, 1:8-16 Bailey TL, Rivara CB, Rocher AB, Hof PR: The nature and effects of cortical microvascular pathology in aging and Alzheimer's...

Ngày tải lên: 19/06/2014, 22:20

... (EC00/165) Cells were routinely cultured in Dulbecco’s modified Eagles media (DMEM) containing 10% fetal calf serum and glutamine (2 mM) and incubated at 37°C in 5% CO2 and 95% air Cells isolated and cultured ... addition and an initiation of a response was significantly slower with higher concentrations of isoproterenol Methods Human Airway Smooth Muscle Cell culture Human airway smooth muscle (hASM) cells ... HEK293-GloSensor cells and human bronchial smooth muscle cells both endogenously expressing b2-adrenoceptors Rosethorne and colleagues reported a correlation between intrinsic agonist efficacy and rate...

Ngày tải lên: 12/08/2014, 13:22

... asthma and COPD include the production and release of chemokines and cytokines by inflammatory and structural cells [3] Airway smooth muscle cells have recognized as immunomodulatory cells able ... from human airway smooth muscle PKA and Epac have been reported to modulate GTP-loading of the Ras-like GTPase Rap1 and Rap2 [45,50,51] In hTERT-airway smooth muscle cells, Rap1 and Rap2 were both ... Role of Epac and PKA in GTP-loading of Rap1 and Rap2 Role of Epac and PKA in GTP-loading of Rap1 and Rap2 hTERT-airway smooth muscle cells were fractioned as described in Material and Methods...

Ngày tải lên: 12/08/2014, 14:20

... β-actin and absence of reverse transcriptase (- RT) or RNA (- RNA) dle-shaped cells and, like smooth muscle cells in the medial layer of intact distal human pulmonary arteries, expressed α -smooth muscle ... survival of vascular smooth muscle cells is dependent on the balance between proliferation and apoptosis and the aberrant regulation of these pathways is implicated in proliferative vascular diseases ... activity, expression, and targeting in cells of the cardiovascular system Mol Pharmacol 2003, 64:533-546 Polson JB, Strada SJ: Cyclic nucleotide phosphodiesterases and vascular smooth muscle Annu Rev...

Ngày tải lên: 12/08/2014, 16:20

... subepithelial fibrosis, elevated numbers and volume of mucous cells in the epithelium, increased amounts of airway smooth muscle cells (ASMC), and increased vascularization of the airway wall [7-9] ... increase in ROS, and effect of NAC on EGFR transactivation and MAPK activation Production of ROS has been noted upon growth factor stimulation of arterial smooth muscle cells [43] and bovine tracheal ... inflammation, smooth muscle function, and remodeling J Allergy Clin Immunol 2003, 111(1B):S18-S36 Billington CK, Penn RB: Signaling and regulation of G proteincoupled receptors in airway smooth muscle...

Ngày tải lên: 12/08/2014, 16:20

... airway smooth muscle cells Analysis of chloride transports in rat airway smooth muscle cells A Immunofluorescence study of α -smooth muscle actin in tracheal smooth muscle cells from rat and B ... (COPD) and cystic fibrosis (CF), it is important to understand the mechanisms underlying control of the bronchoconstriction and dilatation Ion channels in epithelial, glandular and smooth muscle cells ... Pharmacological inhibition and immunolocalisation of CFTR in rat tracheal smooth muscle cells Pharmacological inhibition and immunolocalisation of CFTR in rat tracheal smooth muscle cells A Iodide efflux...

Ngày tải lên: 12/08/2014, 16:20

... involved in target antigens Protein extracts are from human umbilical vein endothelial cells and vascular smooth muscle cells Solid lines indicate direct interactions Dashed lines indicate indirect interactions ... Han Y, Long DK, Shen BR, Yan ZQ, Chien S, Jiang ZL: PDGF-BB and TGF-1 on cross-talk between endothelial and smooth muscle cells in vascular remodeling induced by low shear stress Proc Natl Acad ... ng/ml), streptomycin/penicillin (1%) and ciprofloxacin (1%) at 37C in 5% CO2 The VSMC phenotype was confirmed by using smooth muscle myosin heavy chains and and sm22a antibodies (Abcam, Cambridge,...

Ngày tải lên: 12/08/2014, 17:22

... activation from ASMC cultures, that was mediated by mechanisms involving ERK, JNK, PI3-K and AP-1 pathways Therefore, airway smooth muscle cells, similar to vascular smooth muscle cells, are an important ... Figure tissue and of cultures by immunostaining Expressioncell MMP-12 protein in bronchial smooth muscle Expression of MMP-12 protein in bronchial smooth muscle tissue and cell cultures by immunostaining ... metalloproteinase-12 gene expression in human vascular smooth muscle cells Genes Cells 2003, 8:225-234 Townley RG, Horiba M: Airway hyperresponsiveness: a story of mice and men and cytokines Clin.Rev.Allergy...

Ngày tải lên: 12/08/2014, 18:20

... particles, and CD23b is induced by IL-4 and is also found on non- B cells such as T cells, Langerhan's cells, monocytes, macrophages, platelets, and eosinophils [12,13] IL4 causes CD23 induction on B cells ... bronchial smooth muscle, both hypertrophy [2] and hyperplasia [3], plays a critical role in the development of airway hyperreactivity (AHR), the hallmark of asthma Airway smooth muscle cells (ASMC) ... Membrane ruffling and cell migration involve signaling pathways including PI3kinase, Rac and other Rho family G protein members in a variety of cell types, including vascular smooth muscle cells Rac...

Ngày tải lên: 13/08/2014, 13:22

... Invitrogen Invitrogen Biontex Invitrogen Gene Therapy Solutions Invivo and µg and µg 0.8 and 1.6 µg and µg and µg and µg and µg 3:1 3:1 3:1 3:1 4:1 5:1 3:1 3.0 – 16.4 0.4 – 34.0 3.4 – 18.3 0.6 ... both high efficiency and acceptable survival rate for both smooth muscles cells and endothelial cells (Table 2) Enhancement of transfection efficiency by transfection reagents and the ECM 630 instrument ... Coronary Artery (#CC-2585) and Aortic [#CC-2535] EC The cells had been isolated from normal human tissue and cryopreserved in smooth muscle cell media, SmGM-2 (#CC3182) and endothelial cell media,...

Ngày tải lên: 14/08/2014, 19:22

... of smooth muscle cells Atherosclerosis is a disease that involves multiple cell types, including endothelial cells, macophages, lymphocytes, and smooth muscle cells (SMC) (80) Smooth muscle cells ... metalloproteinase (MMP9) in primary smooth muscle cells via regulation of NFκB family member p65 Materials and Methods Cells, Antibodies, and Reagents Human coronary artery smooth muscle cells (HCASMC) are purchased ... found that VCAM1 and ICAM1 are expressed by intimal smooth muscle cells in human and mouse atherosclerotic coronary, aorta, and carotid arteries, but not in healthy medial smooth muscle (33, 57,...

Ngày tải lên: 24/08/2014, 11:25

... pulmonary artery smooth muscle cells (PASMCs) (vascular smooth muscle cells showing some differences from systemic vascular smooth muscle cells) If so, whether and how HO-1 induction further inhibits ... endothelial cells and human umbilical artery or vein smooth muscle cells [13] However, it is still unknown whether activation of PPARc also stimulates HO-1 expression in pulmonary artery smooth muscle cells ... (5-HT)] and endothelin-1 [14,15] Vascular smooth muscle cells are normally quiescent, and remain in the G1 ⁄ G0 phase of the cell cycle However, upon stimulation, cells exit the G1 ⁄ G0 phase and...

Ngày tải lên: 15/03/2014, 10:20