3 isolation and characterization of progenitor cells

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... represent averages of two values ND, not detectable (e) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml anti-CD3 antibody and the indicated number of mitomycin ... upregulate expression of PD-L1, iNOS, and COX-2 in MSCs, making these molecules candidate mediators of T-cell inhibition The involvement of iNOS and COX-2 in inhibition of T-cell proliferation...

Ngày tải lên: 12/08/2014, 11:23

... Derivation of HES cells 15 1.5.2 Expansion and maintenance of pluripotency of HES cells 16 1.5.3 Characterization of hES cells 18 1.6 Differentiation of hES cells into ... bones of cranial vault, maxilla, and body of the mandible and the midshaft of long bones In this type of ossification, bone develops directly within the soft connective tissue First, mesenchymal cells ... allogenic) and from the bone marrow (mesenchymal stem cells, hematopoietic stem cells) and (2) embryonic stem and embryonic germ cells derived from discarded human embryos and germ line stem cells...

Ngày tải lên: 09/10/2015, 11:24

... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression of EB signature...

Ngày tải lên: 18/06/2014, 15:20

... on the flow cytometry profile after the cells were stained with Hoechst 33342, (B and D): The SP cells in both HCC cells and fetal liver cells disappeared (0.0%) when cells are treated with 50 ... Analysis of stem cell marker expression on the surfaces of SP and non-SP cells The number within each histogram represents the percentage of CD90.1 positive cells (I-K) Quantitative analysis of AFP and ... distinct fraction of SP cells The SP of fetal liver cells was calculated to be 0.15% ± 0.02% (mean ± SEM), and that of HCC cells was calculated to be 0.20% ± 0.08% Once identified, the cells in the...

Ngày tải lên: 10/08/2014, 10:20

... wavelength [nm] Means and SEM ofto investigation Figure 80–270 prior tumor tissue spectra and normal tissue spectra after inhalation of 200 mg of 5-ALA in the time range from Means and SEM of tumor tissue ... relation to histology results Sensitivity and specificity of fluorescence bronchoscopy and Sensitivity and specificity of fluorescence bronchoscopy and white-light bronchoscopy in relation to ... expected between 110 and 160 after inhalation of 5-ALA [13] Our results favor the performance of fluorescence bronchoscopy within a time period between 80 and 270 after the inhalation of 5-ALA, with...

Ngày tải lên: 12/08/2014, 15:20

... showed that four out of four and three out of four animals seroconverted after infection with A663 and N569 BoHV-5 strains, respectively In addition, three out of four and two out of four animals ... lysis and infection plaque size were assessed The one-step growth kinetics curves profiles for the N569 and A663 strains of BoHV-5 were established Total fractions curves showed similar profiles and ... Veterinaria Scandinavica 2011, 53:37 http://www.actavetscand.com/content/53/1/37 Page of Table Viral titres shedding after experimental infection of calves (n = 8) with N569 and A663 strains of bovine...

Ngày tải lên: 12/08/2014, 18:22

... integration value of acetyl protons at 1.8–2.2 ppm and B is that of OH protons and H-1 to H-6 protons of pullulan moiety observed at more than 3.5 ppm Preparation and characterization of PANs and PA/EPI ... stability and toxicity of PANs, and further investigated the sustained release behavior in vivo of drug loading in PANs PA was firstly synthesized and characterized by FT-IR and 1H NMR, and then ... use only Characterization of PANs and PA/EPI NPs in 10% FBS, which had a composition very similar to the body liquids The ζ potentials of PANs and PA/EPI in 10% FBS were −1.460 ± 0.297 and −1.902 ± 1.112 mV...

Ngày tải lên: 23/04/2013, 21:38

... Effect of addition of mM DNP on cell growth (h) and expression of UP12 (j) (B) Monitoring of cell growth (s) and amount of UP12 (d) before and after addition of 0.1 mM CCCP (C) Effect of temperature ... with GroEL from 15 and 30 lg of the cell lysate without (lanes and 3) or in the presence of ATP (lanes and 4) was detected with anti-UP12 Ig Samples (3 and lg of total proteins) of the cell extract ... cell growth and expression of UP12 Absorption (upper panel) and relative amount of UP12 (lower panel) in samples of cells grown in minimal M9 medium containing limited concentration of glucose...

Ngày tải lên: 22/02/2014, 07:20

... presence of lm Ga-PP, and in parallel in the absence of any porphyrin compound and in the presence of lm Fe-PP, respectively, as controls The cytoplasmic fraction was isolated from the cells and analysed ... preparations of isolated Cu-PP-KatA and Mg-PP-KatA, which contained only low amounts of metals; Cu-PPKatA contained 0.04 mol of copper per mol of KatA polypeptide, and Mg-PP-KatA contained 0.02 mol of ... characterize and analyse the amount of Fig Fluorescence emission spectra of PP, Mg-PP, Cu-PP, and Zn-PP, and of the porphyrins contained in isolated PP-KatA, Mg-PPKatA, Cu-PP-KatA, and Zn-PP-KatA...

Ngày tải lên: 06/03/2014, 11:20

... representation of candidate model, Model I, for the GAL genetic switch in the presence of galactose D1 and D2 represent genes with one and two binding sites, respectively G4, G80, G3 and G3* represent ... sensitivity of protein expression to galactose and additionally report Hill coefficients of 1.2 and for one and two binding sites, respectively The equations representing the four candidate models ... yield f1p and f2p at various galactose concentrations Figure shows comparisons of f1p and f2p between the model simulation results for candidate Model I and experimental data The formation of the...

Ngày tải lên: 07/03/2014, 16:20

... lymph node proteins revealed a radioactive band of  25 kDa in liver and bands of  30 and 56 kDa in thoracic lymph nodes (Fig 5C) No radioactive protein bands could be detected in spleen samples ... embryotoxicity of selenite and selenate: uptake in murine embryonal and fetal tissues and effects on blastocysts and embryonic cells in vitro Toxicology 63, 123–136 3459 In vivo tracking of 75 Se-labelled ... (A) and autoradiogram (B) of the same SDS ⁄ PAGE Proteins from lymph node (LN) of a sensitized mouse (one representative experiment out of two) and liver of sensitized and nonsensitized mice shown...

Ngày tải lên: 07/03/2014, 21:20

... II (PSII) of C reinhardtii [16] We now report the expression of His- and Strep-tagged plant PSI Results A series of cassettes containing tagged variants of the full-length precursor of Arabidopsis ... prepared from pools of plants expressing similar levels of PSI-G, and analysed by immunoblotting, using antibodies to PSI-G and PSI-F, as an indicator of the relative content of PSI in the samples ... upper band (Fig 6B) and we have no reason to believe that the lower band is a degradation product of the tagged versions of PSI-G Cross-contamination with wild-type PSI-G during the process of preparing...

Ngày tải lên: 07/03/2014, 21:20

... followed the pattern of p53 and c-Jun gene expression showed in Figs and Immunoprecipitation of complex RAR–DNA To elucidate the mechanism of this pattern of expression, the binding of RAR to c-Jun ... to controls Overexpression of c-Jun alters cell cycle parameters and increases the proportion of cells in S, G2 and M relative to G0 phases of the cell cycle The role of c-Jun in promoting cell ... opposite expression pattern to that of the p53 gene RT-PCR analysis of c-jun, p53 and p21WAF1/CIF1 in liver and lung of control, chronic vitamin A-deficiency and hypervitaminosis rats Based on...

Ngày tải lên: 08/03/2014, 08:20

... (sodium salt) and 2–5 lg of membrane protein in a total volume of 240 lL Assays were run for 10 at 37 °C Under these conditions, the concentrations of MgATP and free Mg2+ were 2.5 mM and 0.5 mM, ... use of mutants and inhibitors acting on the same biosynthetic points has proved to be a useful way of distinguishing the influence of direct effects, i.e sterol alteration, and indirect effects of ... basis of the antifungal activity of N-substituted imidazoles and triazoles Biochem Soc Trans 11, 665–667 20 Burden, R.S., Cooke, D.T & Hargreaves, J.A (1990) Mechanisms of action of herbicidal and...

Ngày tải lên: 08/03/2014, 22:20

... factor, and thus different levels of primary transcript and mature miRNA were shown to occur simultaneously in living cells Therefore, the simultaneous monitoring of production of both pri-miRNA and ... vector controls and represents the degree of miRNA23 production in HeLa cells (A, upper), 293 cells (B, upper) and P19 embryonic carcinoma cells (C, upper) Simultaneous monitoring of the bioluminescence ... unbalanced pattern of production during biogenesis, of a large amount of primiRNA and a low level of mature miRNA, without being processed by the RNase III enzyme Drosha, and that no correlation...

Ngày tải lên: 16/03/2014, 01:20

... homeostasis A B Fig Levels of NOS isozymes and HSP90 in brain and aorta of NMS rats treated with HSD and C.I.1 Aliquots of brain soluble material (20 lg protein) and of aorta total lysate (50 ... formation and stabilization of proteolytically resistant complexes of NOS with HSP90 are crucial in determining the in vivo resistance of NOS and HSP90 to calpain degradation Results Levels of HSP90 and ... brain and aorta The level of HSP90 and the type of NOS isoform present in rat brain and aorta were determined by immunoblotting (Fig 1) In brain, nNOS was the most preferentially expressed isoform,...

Ngày tải lên: 30/03/2014, 04:20

... samples of the ischemic and non-ischemic HL were collected and frozen with liquid nitrogen and then were homogenized and digested; the microspheres were collected and suspended in N,N-dimethylthioformamide ... instead, a result of growth and positive remodeling of pre-existing vessels, forming larger conduits and collateral bridges between arterial networks via recruitment of smooth muscle cells Unlike ... Figureof VEGF15, VEGF165 and QK on the vasomotor responses of 12 common carotid arteries from normotensive rats (A) Effects Effects of VEGF15, VEGF165 and QK on the vasomotor responses of 12...

Ngày tải lên: 18/06/2014, 15:20

... monitor morphology and the ratio of glandular epithelium and interstitium Exclusively samples characterized by high amount of glandular epithelium, over 80%, were used for RT-PCR and immunohistochemistry ... understanding of their role in the spatial and temporal organization of either active or silent protein complexes is of relevance in the context of both general developmental processes and, especially, ... the basis of the results of immunohistochemical evaluation of expression of the estrogen and progesterone hormone receptors and the ErbB-2 receptor kinase, cytokeratins and the proliferation marker...

Ngày tải lên: 18/06/2014, 16:20

... subjects between 32 and 45 years of age (two left and two right, average height of 175 cm and average body mass index (BMI) of 21.8 kg/m2) were included in this study None of the participating ... (b) 3D model of the AJC with anterior talofibular ligament (ATFL) and calcaneofibular ligament (CFL) Page of (page number not for citation purposes) Journal of Orthopaedic Surgery and Research ... O: Stability of the ankle joint Analysis of the function and traumatology of the ankle ligaments Acta Orthop Scand Suppl 1985, 211:1-75 Rasmussen O, Andersen K: Ligament function and joint stability...

Ngày tải lên: 20/06/2014, 01:20