isolation and characterization of human fetal myoblasts

... mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth measured ... Graduate Program, University of Minnesota, Minneapolis, Minnesota, USA and 2Dept of Genetics, Cell Biology and Development and the Institute of Human Genetics, University of Minnesota, Minneapolis, ... X-chromosome and APRT human chromosome 16) are diploid and possibly polyploid in HeLa cells [14] Table shows the kinetics of the appearance of 6-TG and DAP resistant colonies after rounds of mutagenesis...

Ngày tải lên: 13/08/2014, 05:22

... replaced with alanine and valine, respect- ively, and the resultant V NAR s evaluated for retention of binding affinity. Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase ... the possibility of inter- and intra CDR disulphide cross-links. These strategies are summarized in Fig. 1B and Table 1, and details of the library construction are given in greater detail in the Materials and ... possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys). Use of alanine and valine...

Ngày tải lên: 17/03/2014, 10:20

... above, a study of isolation and characterization of a novel human gene was initiated The objectives of this... publication of a working draft of the human genome sequence ... ISOLATION AND CHARACTERIZATION OF THE NOVEL HUMAN GENE, MOST-1 JEANNE TAN MAY MAY (B.Sc. (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY ... reverse bands (R-bands) of metaphase... development and • large-scale protein analysis; And the comparative genomics; which will encompass the complete sequencing of model organisms and...

Ngày tải lên: 16/09/2015, 17:13

... specific antigen and cytokeratin 18 and the presence of microfilaments and secretary vesicles, microvilli, tight junctions and desmosomes Anchorage independent growth, invasion of matrigel coated ... animal models, cell lines and biopsies from the tumor itself Although they all provide great information and enabled outstanding discoveries to understand the disease and to develop powerful treatments; ... 17:803 DOI 10.1186/s12885-017-3812-5 RESEARCH ARTICLE Open Access Isolation and characterization of a new naturally immortalized human breast carcinoma cell line, KAIMRC1 Rizwan Ali1,2, Nosaibah...

Ngày tải lên: 06/08/2020, 03:27

... Journal of Water and Environment Technology, Vol.3, No.2, 2005 Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge Junichi Tsuge1 and Masuo ... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular...

Ngày tải lên: 05/09/2013, 09:08

... similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of identity expressed in Fig. 3. (Continued). Characterization of four pulchellin isoforms ... cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for 5 min at 65 °C. Subsequently, 4 lL of the first strand buffer 5 X and 2 lL of dithiothreitol ... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol....

Ngày tải lên: 18/02/2014, 16:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), confirming its hetero-oligomeric nature (Fig. 1, lane 1). However, in the absence of boiling (Fig. 1A, lanes 2 and 4) ... The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers. All of the bands stained positively...

Ngày tải lên: 21/02/2014, 00:20

... protein of Brassica, spinach, barley and A. thaliana,PR1ofPhaseolus and MHF 9 of A. thaliana. These proteins b elong to the 2Cys-Prx subfamily. A ll plan t 2Cys-Prx proteins, except BAS1 of barley, ... (ROS), p rotection of DNA against degradation, its p eroxidase a ctivity, and its ability t o u se different thioredoxin isoforms as hydrogen donors. To better understand the function of Ch-Prx1 in ... SDS, and 1% BSA. After p uri®cation of positive clones, k DNA was extracted and puri®ed as described in [ 13]. cDNA inserts were excised by a digestion with EcoRIandclonedintotheEcoR I site of...

Ngày tải lên: 08/03/2014, 16:20

... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation. Results In silico characterization and isolation of the Arabidopsis ... presequence of 19 amino acids. The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34. The YedO protein from E. coli and the d-CDes from Arabidopsis showed an overall identity of ... (Figs 3 and 4). In general, the localization predictions of plastidic and mitochondrial proteins are correct for only about 50% of all plant proteins [25]. Because of this high degree of uncer- tainty...

Ngày tải lên: 16/03/2014, 18:20

... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of ... Utilisation of the budding yeast Saccharomyces cerevisiae for the generation and isolation of non-lethal ricin A chain variants Yeast 22, 1287–1297 16 Gemmill TR & Trimble RB (1999) Overview of N- and...

Ngày tải lên: 23/03/2014, 07:20

... of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails. A ... ligand, decapentaplegic (dpp), during anterior–posterior patterning of the wing [22]. Shn mutants mimic a large number of dpp loss -of- function phenotypes and mutations in the Dpp-receptors tkv and punt ... expression of brk [27]. This silencer is regulated directly by a complex consisting of Mad/Medea and Shn. While the fundamental aspects of TGF-b signaling are highly conserved and the requirement of...

Ngày tải lên: 30/03/2014, 13:20

... increases in temperature, band Y faded, whereas the intensity of band Z increased; with heat treatment at 100°C, only band Z was observed. The sizes of proteins in bands X, Y, and Z were estimated ... proteins in these bands accounted for 80% of the total viral proteins. By in creasing the temperature of the heat treatment to 70°C, band X faded, whereas the intensity of band Y increased. With ... content of CvV-BW1 is in the vicinity of 41.3%. Figure 7 Restriction enzyme digestion of CvV-BW virion genomes. Figure 8 Restriction enzyme digestion of the CvV-BW1 genome. For band sizes of the...

Ngày tải lên: 12/08/2014, 01:21

... individuals and organizations produce and reproduce their material subsistence and organize time and space They are also symbolic systems, ways of ordering reality, and thereby rendering experience of ... pricing of children (Zelizer, 1994), and attempts to purchase tribal land (Espeland, 1998) In the context of symphony orchestras, we use the notion of market logic to refer to broader notions of self-interest ... critics may expand the scope of their commentary and evaluation to include aspects of orchestral marketing, audience instruction, use of metaphor and analogy from other genres, and non-aesthetic...

Ngày tải lên: 30/03/2014, 16:20

... ASTM Annual Book of Standards, Part 31, D3694-78 “Standard Practices for Preparation of Sample Containers and for Preservation of Organic Constituents,” American Society for Testing and Materials, ... levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask and diluting to volume with acetonitrile One of the external standards should be at a ... calibration standards at a minimum of three concentration levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask To each calibration standard,...

Ngày tải lên: 14/02/2014, 03:20

... and 6.7 It is recommended that the secondary dilution standard be prepared at a concentration of 15 µg/mL of each internal standard compound The addition of 10 µL of this standard to 5.0 mL of ... concentration of the stock standard Optionally, stock standard solutions may be prepared using the pure standard material by volumetrically measuring the appropriate amounts and determining the weight of ... program The minimum requirements of this program consist of an initial demonstration of laboratory capability and an ongoing analysis of spiked samples to evaluate and document data quality The...

Ngày tải lên: 14/02/2014, 03:20

... The addition of 10 µL of this solution of mL of sample or standard is equivalent to a concentration of 30 µg/L of each surrogate standard 6.8 BFB Standard—Prepare a 25 µg/mL solution of BFB in methanol ... 51, and 86 101 103 96 61 and 98 63 65, 83, 85, 98, and 100 96 61 and 98 83 85 98 62, 64, and 100 97 99, 117, and 119 117 119 and 121 127 83, 85, and 129 112 63, 65, and 114 75 77 130 95, 97, and ... 15-40% of Mass 95 30-60% of Mass 95 Base Peak, 100% Relative Abundance 5-9% of Mass 95 50% of Mass 95 5-9% of Mass 174 >95% but 101% of Mass 174 5-9% of Mass 176 Table...

Ngày tải lên: 05/03/2014, 21:20

... “Microdetermination of Derivatives of Phenols and Mercaptans by Means of Electron Capture Gas Chromatography,” Analytical Chemistry, 40, 1009 (1968) ASTM Annual Book of Standards, Part 31, D3694-78 “Standard ... calibration standards at a minimum of three concentration levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask To each calibration standard, ... known constant amount of one or more internal standards, and dilute to volume with 2-propanol One of the standards should be at a concentration near, but above, the MDL and the other concentrations...

Ngày tải lên: 05/03/2014, 21:20

... levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask and diluting to volume with mobile phase One of the external standards should be at a ... calibration standards at a minimum of three concentration levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask To each calibration standard, ... known constant amount of one or more internal standards, and dilute to volume with mobile phase One of the standards should be at a concentration near, but above, the MDL and the other concentrations...

Ngày tải lên: 14/03/2014, 20:20

... levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask and diluting to volume with hexane One of the external standards should be at a concentration ... calibration standards at a minimum of three concentration levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask To each calibration standard, ... a known constant amount of one or more internal standards, and dilute to volume with hexane One of the standards should be at a concentration near, but above, the MDL and the other concentrations...

Ngày tải lên: 14/03/2014, 20:20

... materials Nitrosamine reference standards and standard solutions should be handled and prepared in a ventilated glove box within a properly ventilated room Apparatus and Materials 5.1 Sampling equipment, ... levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask and diluting to volume with methanol One of the external standards should be at a concentration ... calibration standards at a minimum of three concentration levels for each parameter of interest by adding volumes of one or more stock standards to a volumetric flask To each calibration standard,...

Ngày tải lên: 22/03/2014, 14:20