design rna isolation and microarray hybridization

... D-AT, NF, and AR designed the research; MY, JP, JZL, XA, D-AT, NF, and AR performed research; MY, NF, and AR analyzed data; JZL and XA contributed text to the methods section; and MY, NF, and AR ... used strand-specific RT-PCR and sequencing to validate the presence of the orthologous sense and antisense transcripts in each species in mid-log and early stationary phase, and used strand-specific ... strand cDNA synthesis started RNA denaturation and the hybridization of the pmol of gene specific primer Total RNA with primer (10 ng) was heated to 70°C for 10 minutes and incubated on ice for at...

Ngày tải lên: 09/08/2014, 20:22

... CN, EK, IZ and LM were responsible for study design and data collection MB, BS and JK performed the statistical analyses JC and EK performed the laboratory analyses JK, MB, EK, CW, JC and AM drafted ... albumin and hsCRP, and the hazard of death was non-linear (p < 0.10), and there was evidence that the association between albumin and the hazard of the combined endpoint of mortality and loss ... albumin, and 58 hsCRP and ferritin values) using multiple imputation techniques [19] The proportion of imputed values was within the commonly accepted range [19] Koethe et al Journal of the International...

Ngày tải lên: 20/06/2014, 08:20

... between and 14 kDa, and are characterized by two calcium binding sites of the EF-hand type (helix-loop-helix) [20] S100A8 and S100A9, generally functioning as the S100A8/A9 heterocomplex, and S100A12 ... relationship to joint and vascular damage and risk factors in RA patients [19] We report associations of each protein with both joint and vascular disease and their risk factors Materials and methods Subjects ... design, acquisition, analysis and interpretation of data WY and CG carried out S100A8, S100A9 and S00A12 assays YC, CG, MB and RT wrote the manuscript All authors read and approved the final manuscript...

Ngày tải lên: 09/08/2014, 13:22

... and supervised the analysis of ions and elements ZB performed the ion and element analyses MAM advised on the collection and interpretation of the data PMH developed the experimental design and ... h at 4°C and then both breast muscles (m pectoralis) and both whole thighs (bone and muscles) were cut from the carcass and evaluated for muscle colour, lightness (L*), redness (a*) and yellowness ... in a cold muffle furnace and heated to 550 ± 5°C for 16 h (overnight) After this time, the beakers and their contents were removed from the furnace, placed in a desiccator and allowed to cool...

Ngày tải lên: 14/08/2014, 13:21

... social class and socioeconomic factors like childhood poverty and family income, low parent education, and the more limited opportunities and negative influences faced by minority children And indeed, ... Administration of Children and Families (ACF) of the U.S Department of Health and Human Services This involved designing and field testing one-on-one child assessments and developing a computer-based ... Representatives to design and produce three editions of the Committee's report, U.S children and their families: Current conditions and recent trends He was a member of the National Committee on Vital and Health...

Ngày tải lên: 28/03/2014, 09:20

... changes in mood and behavior were to be considered, novel codes and standards that influence the choice of lighting technologies and the design of indoor environments could be developed and implemented ... architectural and design solutions, and the seasonbound changes in mood and behavior can be alleviated or even prevented with the use of scheduled light exposures [17] These practices may converge, and ... two-stage stratified cluster sampling design was planned by Statistics Finland The sampling frame comprised adults aged 30 years and over living in mainland Finland This frame was regionally stratified...

Ngày tải lên: 18/06/2014, 19:20

... incontinence) and search (quality of life and bother and urinary Results of search (quality of life and distress and urinary incontinence) and search (quality of life and bother and urinary incontinence) ... The first search used the terms "quality of life and distress and urinary incontinence" and the second used the terms "quality of life and bother and urinary incontinence." The searches were limited ... incontinence episodes and total UDI and IIQ scores [55] Relationships between UDI and IIQ were examined at baseline and post-operatively One study reported a moderate correlation between UDI and IIQ at...

Ngày tải lên: 18/06/2014, 19:20

... Conclusion RNA extraction The QIAamp Viral RNA mini kit (Qiagen/Westburg, Leusden, The Netherlands) was used according to the manufacturer's instructions for the extraction of rotavirus RNA from ... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... of the isolates and became the most prevalent genotype, and the number of untypeable strains was reduced from 36.9 to 2.1 % The other common G types were G9 (21.7%), G2 (15.0%), and G4 (13.8%)...

Ngày tải lên: 19/06/2014, 08:20

... triglycerides and lower LDL than healthy controls [20] However, only limited information is available on the effect of serum triglycerides and cholesterol levels and the roles of HDL and LDL levels ... lipid profile and clinical status The standardized acquisition and analysis methods for obtaining contrast-enhancing lesion volume (CE-LV), CE lesion number (CEL number), T2-LV, T1-LV and brain parenchymal ... EDSS < 4.0 and ≥ 4.0 The baseline EDSS groups were analyzed using logistic regression with sex as a factor and disease duration and lipid profile variable of interest The CE-LV, T2-LV and T1-LV...

Ngày tải lên: 19/06/2014, 22:20

... GF, ZsI and PG conceived of the study, and participated in its design and coordination, and helped to draft the manuscript; L-MT and M-OS carried out the immunoassays; GSZ, T, GP, KH, RSZ and ZSz ... ficolin-3 and high CRP levels on the one hand and the unfavorable outcome of the disease on the other hand, we repeated the analysis as above in the follow-up samples by including ficolin-3 and CRP ... ficolin-3 on the one hand and the severity of ischemic stroke and the infarct size on the other hand, as discussed above It is well known that both the high baseline NIHSS score and the high serum...

Ngày tải lên: 19/06/2014, 22:20

... Detection and genotyping of human group A rotaviruses by oligonucleotide microarray hybridization J Clin Microbiol 2002, 40:2398-2407 Lovmar L, Fock C, Espinoza F, Bucardo F, Syvanen AC, Bondeson K: Microarrays ... [17,22-24] and one for P-typing [25] In the one developed by Gouvea et al [24], it was reported failures in the correct genotyping of G2, G8, G9 and G10 rotavirus strains [7,12,17,20,21], and In ... corrections of the manuscript, and Juan Stupka and the three anonymous referees for critical corrections 20 References Parashar UD, Gibson CJ, Bresee JS, Glass RI: Rotavirus and severe childhood diarrhea...

Ngày tải lên: 20/06/2014, 01:20

... Conclusion RNA extraction The QIAamp Viral RNA mini kit (Qiagen/Westburg, Leusden, The Netherlands) was used according to the manufacturer's instructions for the extraction of rotavirus RNA from ... laboratory tests and wrote the manuscript; RS and GP carried out RT-PCR tests; JM performed the sequencing experiments; AF, KZ, RB and DS supervised the rotavirus surveillance program and critically ... of the isolates and became the most prevalent genotype, and the number of untypeable strains was reduced from 36.9 to 2.1 % The other common G types were G9 (21.7%), G2 (15.0%), and G4 (13.8%)...

Ngày tải lên: 20/06/2014, 04:20

... conceptualized and designed the experiments, collected, assembled, analyzed and interpreted data, and drafted the manuscript OC conceptualized and designed the experiments, and collected, assembled and ... specimens, and analyzed and interpreted data PD conceptualized and designed the experiments, analyzed and interpreted data, and helped with the drafting of manuscript JM conceptualized and designed ... designed and implemented experiments SS recruited, collected and annotated specimens, and interpreted data KO recruited, collected and annotated specimens, and interpreted data SC collected and...

Ngày tải lên: 20/06/2014, 08:20

... the internode and petiole angles and azimuths, the angle and azimuth of the leaf surface normal, and the azimuth of the midrib were recorded with a compassprotractor Leaf, petiole, and internode ... and bibliography of Fagales (Betulaceae, Corylaceae, Fagaceae and Ticodendraceae), The Royal Botanic Gardens, Kew, 1998 [15] Grassi G., Giannini R Influence of light and competition on crown and ... Photoinhibition and drought in Mediterranean woody saplings: scaling effects and interactions in sun and shade phenotypes, J Exp Bot (2005) 56: 483–494 [49] Waller D.M., The dynamics of growth and form...

Ngày tải lên: 07/08/2014, 16:20

... endostatin and bFGF and other clinical parameters No correlation of VEGF, endostatin and bFGF levels with skin score, carbon monoxide diffusion capacity and the presence of teleangiectasias and other ... (median, 453 pg/ml; range, 93–1143 pg/ml) and patients without detectable autoantibodies (P = 0.36) Autoantibodies and bFGF and endostatin levels Disease duration and VEGF levels To examine whether ... (Scl-70 neg) and higher levels than patients without detectable autoantibodies Data are shown as box plots, with upper and lower quartiles shaded # P < 0.05 Capillaroscopy and endostatin and bFGF...

Ngày tải lên: 09/08/2014, 06:22

... and clinical findings, the objective of this study was to explore the relationships of KRAS and BRAF status with p-AKT and p-ERK expression and with clinical outcomes (response to radiation and ... results, and drafted the manuscript BFC participated in the study development and data analysis JET participated in the study design, data interpretation and manuscript revisions All authors read and ... cetuximab[36] to 23% in combination with irinotecan, [37] and disease control rates (a combination of complete and partial responses and stable disease) of 39 and 56% respectively Preclinical studies have...

Ngày tải lên: 09/08/2014, 09:21

... Surgery and Internal Medicine, the nurses and physician assistants at the Department of Gastroenterology, the research nurses at the Department of Rheumatology and Clinical Immunology, and the ... actual and recent medication, co-morbidity and medical history The questionnaire contained specific items on the use of NSAIDs, aspirin, anticoagulants, gastroprotective drugs, and steroids, and ... writing the article HEV and RWF also contributed to the selection and inclusion of cases and controls JvdP also contributed to the statistical analysis of the data All authors read and approved the...

Ngày tải lên: 09/08/2014, 10:20

... TJH, CMTG, and GSC participated in study design DG, JP, CAP, CDS, JGH, CP, and GB participated in recruitment of patients and their family members, and acquisition of clinical data and laboratory ... NKT cell numbers and function, and that these changes precede the development of disease Aside from the changes in NKT cell numbers and the proportions of memory and/ or naïve B and CD4+ T cells, ... studies, and data acquisition SL, NR, CMTG, and ADP performed the statistical analyses TM and JOC coordinated the acquisition of laboratory samples, clinical data acquisition, and entry of clinical and...

Ngày tải lên: 09/08/2014, 13:21

... 0.965 and Correlation coefficient r = 0.872 for rs6990375 G>A and rs3802278 G>A; D’ = 0.981 and r2 = 0.678 for rs6990375 G>A and rs3087714 C>T; D’ = 1.000 and r2 = 0.919 for rs3802278 G>A and rs3087714 ... information on the study design and edited the manuscript QW advised on study design, and revised the manuscript preparation, and supported the study L-EW participated in the study design, oversaw the ... experiment in cell lines and participated the analysis and manuscript preparation KHL provided patients’ samples and clinical information ZL advised on designing primers and helped laboratory experiments...

Ngày tải lên: 10/08/2014, 10:20

... experiments and carried out the majority of the assays, performed the statistical analysis, participated in the design and coordination of the study, and drafted the manuscript Brodsky et al Journal ... its coordination and drafted the manuscript BS conceived of the study, participated in the design and coordination of the study, and drafted the manuscript All authors read and approved the final ... pro-inflammatory cytokines and inhibit iNOS expression and NO release in LPS-stimulated RAW264.7 macrophages by targeting the NFB activation pathway Materials And Methods Cell Culture and Sample Treatment...

Ngày tải lên: 11/08/2014, 08:22