Báo cáo khoa học: Light-induced reactions of Escherichia coli DNA photolyase monitored by Fourier transform infrared spectroscopy pot

... spectra of DNA photolyase. (A, A¢) Photore- duction of DNA photolyase (two different batches of protein). (B) Photoreduction of [U- 15 N] -DNA photolyase. (C) Photoreduction of DNA photolyase ... difference spectra of DNA photolyase and DNA. (A) Oligo-(dT) 18 DNA photodamage with UV radiation in the absence of photolyase. (B) Photoreactivation of DNA ph...

Ngày tải lên: 16/03/2014, 18:20

... Activation of the acetyl phosphate synthesis activity of E. coli Pta by different concentrations of pyruvate and PEP. Results are presented as percentage of activity in the presence of PEP or ... primer, 100 ng of DNA template, 5 lL of 5· GoTaq DNA polymerase buffer, and 0.6 U of GoTaq DNA polymerase (Promega, Madison, WI, USA). The amplification protocol was as follo...

Ngày tải lên: 06/03/2014, 11:20

... only treatment of CTPS with trypsin produced a limited number of cleavage fragments over the course of 1 h, of which the formation of some fragments was suppressed in the presence of ATP and/ or ... hydrolysis of glutamine, but had a value of k cat thatwashalfofthat observed for wild-type CTPS in the absence of GTP. When the concentration of GTP was increased to 1 m M ,the...

Ngày tải lên: 23/03/2014, 17:22

... HT29-5F7. The presence of non-GM1 recep- tors of Escherichia coli heat-labile enterotoxin (LT-I) on both types of dif- ferentiated HT29 cells was indicated by the inability of cholera toxin B subunit ... K-A (1994) Comparison of the glycolipid-binding specificities of cholera toxin and porcine Escherichia coli heat-labile enterotoxin: identification of a receptor-active...

Ngày tải lên: 30/03/2014, 10:20

... phos- photransferase system (PTS) in Escherichia coli is one of the best studied biochemical networks from theo- retical and experimental points of view, and has Keywords Escherichia coli; model verification; ... modeling of the central carbon metabolism of Escherichia coli. Biotech- nol Bioeng 79, 53–73. 8 Mahadevan R, Edwards JS & Doyle FJ (2002) Dynamic flux balance ana...

Ngày tải lên: 18/02/2014, 13:20

... was investigated by monitoring the growth of flask cultures of R. rhodochrous B-276 in the presence of a range of alkynes (Fig. 1). A fivefold molar excess Adventitious reactions of alkene monooxygenase ... regeneration of NADH. In the context of a whole-cell biocatalyst that would bypass problems with coenzyme regeneration, we began the present study of AMO by investigati...

Ngày tải lên: 16/03/2014, 19:20

... BamHI–EcoRI sites of pGST–pHis, a cDNA fragment encoding amino acids 1–165 of calpain 7 (calpain 7MIT) was amplified by using a pair of primers (forward, 5¢-GAG AGATCT Enhancement of calpain 7 autolysis by IST1 ... were proba- bly derived by limited digestion with other cellular pro- teases, and not by autolysis of mGFP–calpain 7. Enhancement of autolysis of mGFP– calpain...

Ngày tải lên: 18/02/2014, 04:20

... carbonyl group of Asn7 of Tat(1–9) and the side chain of Lys554 of DP IV and finally, between the carbonyl group of Ile8 of the peptide interacting with the Tyr752 hydroxyl group of the enzyme. ... and Asp709 as well as Asp739 of DP IV, between the C-terminal Glu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV. Furthermore, the side chain of Asp5 of Tat...

Ngày tải lên: 20/02/2014, 11:20

... conformation of ETNF-6 mAb Sequencing of the cDNA of the variable region of ETNF-6 mAb was performed, and this was followed by molecular modeling of its three-dimensional struc- ture. The heavy chain of ... the Ser5-Arg6 bond of TNF-a at the first step, resulting in the generation of a fragment of  17 kDa. This fragment was digested to a smaller molecule of 15 kDa by s...

Ngày tải lên: 06/03/2014, 22:21

... inhibitors. Binding of 6PG and 5PR poorly affects the dissociation constant of the coenzymes, whereas binding of 4PE decreases the dissociation constant of the coenzymes by two orders of magnitude. ... enzyme has been obtained by studying the binding of aPyADP in the presence of 6PG [23], and by observing that 6PG enhances the decarboxylation of 3-keto-2-deoxy 6PG, an an...

Ngày tải lên: 07/03/2014, 05:20