... spectra of DNA photolyase. (A, A¢) Photore-
duction of DNA photolyase (two different batches of protein). (B)
Photoreduction of [U-
15
N] -DNA photolyase. (C) Photoreduction of
DNA photolyase ... difference spectra of DNA photolyase and DNA. (A)
Oligo-(dT)
18
DNA photodamage with UV radiation in the absence of
photolyase. (B) Photoreactivation of DNA ph...
... Activation of the acetyl phosphate synthesis activity of E. coli Pta by different concentrations of
pyruvate and PEP. Results are presented as percentage of activity in the presence of PEP or ... primer, 100 ng of DNA template, 5 lL
of 5· GoTaq DNA polymerase buffer, and 0.6 U of
GoTaq DNA polymerase (Promega, Madison, WI, USA).
The amplification protocol was as follo...
... only treatment of
CTPS with trypsin produced a limited number of cleavage
fragments over the course of 1 h, of which the formation of
some fragments was suppressed in the presence of ATP and/
or ... hydrolysis of
glutamine, but had a value of k
cat
thatwashalfofthat
observed for wild-type CTPS in the absence of GTP. When
the concentration of GTP was increased to 1 m
M
,the...
... HT29-5F7. The presence of non-GM1 recep-
tors of Escherichia coli heat-labile enterotoxin (LT-I) on both types of dif-
ferentiated HT29 cells was indicated by the inability of cholera toxin B
subunit ... K-A
(1994) Comparison of the glycolipid-binding specificities
of cholera toxin and porcine Escherichia coli heat-labile
enterotoxin: identification of a receptor-active...
... phos-
photransferase system (PTS) in Escherichia coli is one
of the best studied biochemical networks from theo-
retical and experimental points of view, and has
Keywords
Escherichia coli; model verification; ... modeling of the
central carbon metabolism of Escherichia coli. Biotech-
nol Bioeng 79, 53–73.
8 Mahadevan R, Edwards JS & Doyle FJ (2002)
Dynamic flux balance ana...
... was investigated by monitoring the growth of
flask cultures of R. rhodochrous B-276 in the presence
of a range of alkynes (Fig. 1). A fivefold molar excess
Adventitious reactions of alkene monooxygenase ... regeneration of NADH. In the
context of a whole-cell biocatalyst that would bypass
problems with coenzyme regeneration, we began the
present study of AMO by investigati...
... BamHI–EcoRI
sites of pGST–pHis, a cDNA fragment encoding amino
acids 1–165 of calpain 7 (calpain 7MIT) was amplified by
using a pair of primers (forward, 5¢-GAG
AGATCT
Enhancement of calpain 7 autolysis by IST1 ... were proba-
bly derived by limited digestion with other cellular pro-
teases, and not by autolysis of mGFP–calpain 7.
Enhancement of autolysis of mGFP–
calpain...
... carbonyl group of Asn7 of Tat(1–9) and the
side chain of Lys554 of DP IV and finally, between the
carbonyl group of Ile8 of the peptide interacting with the
Tyr752 hydroxyl group of the enzyme. ... and
Asp709 as well as Asp739 of DP IV, between the C-terminal
Glu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of
DP IV. Furthermore, the side chain of Asp5 of Tat...
... conformation of ETNF-6
mAb
Sequencing of the cDNA of the variable region of
ETNF-6 mAb was performed, and this was followed
by molecular modeling of its three-dimensional struc-
ture. The heavy chain of ... the Ser5-Arg6 bond
of TNF-a at the first step, resulting in the generation of a fragment of
17 kDa. This fragment was digested to a smaller molecule of 15 kDa by
s...
... inhibitors. Binding of 6PG and
5PR poorly affects the dissociation constant of the coenzymes, whereas
binding of 4PE decreases the dissociation constant of the coenzymes by
two orders of magnitude. ... enzyme has been obtained by studying the
binding of aPyADP in the presence of 6PG [23], and
by observing that 6PG enhances the decarboxylation
of 3-keto-2-deoxy 6PG, an an...