... point of view in narrative Computional Linguistics, 20(2): 233 287 T Wilson, J Wiebe, and P Hoffmann 2009 Recognizing Contextual Polarity: an exploration of features for phrase-level sentiment analysis ... 88.06%.2 The distribution of classes in our data set was as follows: 128 1 OBJ, a total of 1574 SUBJ, where 491 were deemed S-POS, 689 S-NEG, and 394 S-NEUT Moreover, each of the sentences in our ... further processing of the morpho-tactics that result from the segmentation of clitics; (2) Lemma, where the stem words are reduced to their lemma citation forms, for instance in case of verbs it is...
... combination of 14-3-3-affinity chromatography and MALDI-TOF MS analysis under 333 4 normal growth conditions in HeLa cells, although confirmation by immunoblot analysis was not performed at the time [12] ... (2010) 332 1 334 2 ª 2010 The Author Journal compilation ª 2010 FEBS 333 7 14-3-3-binding status during apoptosis 10 11 12 13 14 15 16 M Pozuelo-Rubio ultrastructural localization analysisof 14-3-3 ... identification of genuine 14-3-3 ligands Detailed analysisof the 14-3-3-asociated proteins found showed that 46 of them were exclusively present in one of the conditions analyzed and 15 of them were...
... Serial analysisof gene expression: rapid RT-PCR analysisof unknown SAGE tags Nucleic Acids Res 27, e17 Chen JJ, Rowley JD & Wang SM (2000) Generation of longer cDNA fragments from serial analysis ... Science 276, 126 8 127 2 Velculescu VE, Madden SL, Zhang L, Lash AE, Yu J, Rago C, Lal A, Wang CJ, Beaudry GA, Ciriello KM et al (1999) Analysisof human transcriptomes Nat Genet 23, 387–388 Hashimoto ... al (2003) An anatomy of normal and malignant gene expression Proc Natl Acad Sci USA 99, 1128 7– 1129 2 10 Lee S, Zhou G, Clark T, Chen J, Rowley JD & Wang SM (2001) The pattern of gene expression...
... and formation of the oxazolinium ion intermediate; (C) Hydrolysis of the oxazolinium ion intermediate Copyright National Academy of Sciences, USA questioned on the basis of studies of chitinase ... and there is no example of mutational analysisof all of these residues in the same enzyme To obtain an insight into how the many conserved residues in the active sites of family 18 chitinases ... kinetic parameters of Y10F could not be measured at pH 9.0 due to enzyme instability At pH 8.0 Y10F showed a kcat of 0.55 and Km of 47.2 (compared to a kcat of 3.5 and a Km of 24.9 in the wildtype...
... CTCGTACGCTGCAGGTCGAC KU532 KU616 KU617 KU718 KU719 KU1 233 KU1234 KU1235 KU1236 KU1237 KU1009 KU1010 KU1011 KU1 012 KU1130 KU1131 KU1132 KU1 133 fragments of pIB1 ⁄ (construct of PEX1 in pET21d) to plasmid pIB1 ⁄ ... cohort of 31 patients Am J Med Genet 126 A, 333 338 Chevray PM & Nathans D (1992) Protein interaction cloning in yeast: identification of mammalian proteins that react with the leucine zipper of Jun ... biological function of Pex1p and Pex6p 51 Domain function of Pex1p and Pex6p I Birschmann et al A B C D E F Fig Effects of point mutation of the WalkerA and B motifs of the AAA-cassettes of Pex1p on...
... several groups of allosteric interactions: inhibition of both activities of bifunctional AK-HSDH (two isoforms: AKI-HSDHI and AKII-HSDHII) by Thr (G-II), inhibition of the isoforms of monofunctional ... definitions of the coefficients in Doc S1 and [21]) The number of response coefficients in cellular metabolism (number of parameters · number of variables) is very large, and measuring all of them ... the modular representation of the system, there is a small number of explicit variables and of module rates, resulting in a small number of control coefficients This set of control coefficients quantifies...
... packing of the protein tetramers in the crystal cell, which leaves a large amount of empty space, 80% of the volume, filled with solvent The alignment of the amino acid sequence of HP1287 shows 33% ... parameters (A) I 4122 a = b = 148.42, c = 233. 52 125 –2.7 (2.85–2.70) 36057 (5136) 9.4 (8.8) 99.8 (99.0) 9.4 (3.8) 0.081 (0.526) I 4122 a = b = 148.73, c = 233. 57 78–2.4 (2.53–2.40) 5120 0 (7 412) 9.9 (10.2) ... reminiscent of that of human heme oxygenase-1 [13] In the present study, we present the crystal structure of the HP1287 gene product as well as one of its mutants (F47Y) and discuss the in vivo role of...
... modulation of regulatory networks [5,6] A direct benefit of such an approach would be the early recognition of ‘off target’ and side effects of drug candidates [7], as well as the identification of putative ... comparative analysisof signaling pathway(s) events in normal versus diseased tissue, (b) the comparative analysisof protein expression in various systems, (c) elucidation of the dynamic aspects of pathway ... the action of the targeted kinase Because of the inherent potential promiscuity of kinase inhibitors, a more extensive characterization of compound activities across a wide range of signaling...
... Therefore, the structure of this mutant was almost identical to that of the wild-type enzyme, except for the mutation site Mutagenic analysisof aTyr114 To evaluate the effect of aTyr114 on metal ... red and cyan, respectively The Cc2 atom of aThr109 and the Cc1 atom of aVal136 of the Co-type NHase are connected by a yellow line (B) Vicinity of aTyr114 of the Co-type NHase The wild-type, aY114T ... aThr114 residue of the mutant formed a weak ˚ hydrogen bond (3.1 A) with an oxygen atom of the mainchain of aSer 112, as in the Fe-type NHase (Fig 5B) However, the conformation of the cysteine...
... assumed (K2 0.667 [30]) A value of 1.36 was taken for the refractive index n [31] MALDI-TOF analysis MALDI-TOF analysisof the peptides obtained after tryptic digestion of the labeled and nonlabeled ... partial accessibility of the marker to external solvent molecules Binding of palmitate, identified as one of the preferred ligands [12] , caused an additional 25–30 nm blue-shift of R Jordanova et ... MALDITOF analysisof the trypsin digested Ag-NPA-1 [20] Cys66 and Cys122 were found in the peptide fragments of the nontreated, natural protein with theoretically calculated masses of 1 033. 5459 Da (AKESLIGGCR)...
... the method ofanalysis I am describing is not based essentially on a grammatical analysis, as are a number of other systems of semantic analysis [1] The present system takes the notion of meaningful, ... operation of the system is exactly like that of a phrase structure parser, and the resulting interpretation can be thought of as a parsing of the fragments of a paragraph, just as the grammatical analysis ... grammatical analysisof a sentence can be thought of as a parsing of the words constituting the sentence A word of warning is necessary about the odd nature of examples in the field of ambiguity resolution...
... 2008 FEBS 1867 Analysisof upstream region of the Pokemon gene Y Yang et al decoy experiments using lg of the NEG-U decoy, lg of the NEG-D decoy, and a combination of lg each of the NEG-U and ... compilation ª 2008 FEBS 1861 Analysisof upstream region of the Pokemon gene Y Yang et al Fig Nucleotide sequence of the 5¢-upstream region of the Pokemon gene The upstream region of the Pokemon gene ... control expression of the Pokemon gene, a 2204-bp section of the 5¢-upstream region of the Pokemon gene was cloned by PCR using human genomic DNA as the Analysisof upstream region of the Pokemon...
... relationships of cardosins and their plant counterparts The amino acid sequences of C cardunculus APs were compared with those of several other plant APs, by means of the phylogenetic analysis ... their effect on gene expression in transgenic plants Our results clearly show that the removal of kb of the cardo2530 Fig Histochemical analysisof GUS activity in transgenic A thaliana plants transformed ... regions of the genes fused to the reporter Each row of panels represents independent flowers of plant lines transformed with the same construct, at different stages of development The names of the...
... the sensitivity of the rate of the supply module to changes in the concentration of its product and the sensitivity of the rate of the demand module to changes in the concentration of its substrate, ... measured for different ranges of values of S (see Fig of [17]) As a consequence, all values of mean-control coefficients calculated by this analysis involve values of mean-elasticity coefficients ... Authors Journal compilation ª 2006 FEBS 197 Metabolic control analysisof large responses L Acerenza and F Ortega could not be applied to the analysisof the effect of parameters that are related...
... determination of the midpoint reduction potentials of flavin cofactors, and also of the haems in the case of NOS and P450 BM3 In spectroelectrochemical titrations of the isolated domains, the lack of overlapping ... potentiometric analysisof the wild-type and mutant proteins to probe any thermodynamic consequences of mutation; and (b) studies of the kinetics of flavin reduction by reducing coenzymes in the absence of ... nm, with the E12 value being the midpoint of these E1 and E2 values [35]; and that for the FAD domain of P450 BM3; e E12 value cited for the FAD domain of P450 BM3 is the midpoint of the E1 and...
... Higashiyama, H., Hirose, F., Yamaguchi, M., Inoue, Y.H., Fujikake, N., Matsukage, A & Kakizuka, A (2002) Identifica- Analysisof ataxins and (Eur J Biochem 271) 3169 122 123 124 125 126 127 128 129 ... [96 ,120 123 ,125 127 ] VCP binds the ubiquitin E3 ligase and the chain assembly factor UFD2a/E4B, which is a U box homologue of yeast Ufd2 [128 ], and interacts with and regulates the degradation of ... strand) of D3 and I41/L304, C43/A306 (both in b3), L69/S326 and L71/L328 (both in b4) of B Stacking interactions between guanidinium groups of arginines R69/V335 of D3 and R25/G287 and R49/T 312 of...
... chemical shift changes of 0.030.04 p.p.m and 15N chemical shift changes of 0.25 0.35 p.p.m are indicated in orange NH chemical shift changes of more than 0.04 p.p.m and 15N chemical shift changes of ... chemical shift of K35 was greatly changed by addition of SQG Thus, the sulfonyl moiety of SQMG may form a salt bridge to the amino moiety of the sidechain of K35 The hydroxyl moieties of the sugar of ... mM) of SQG (d), MA (n) or a mixture of SQG and MA (s) The DNA polymerase activity in the absence of added compounds was taken to be 100% (C) Gel mobility shift analysis Gel mobility shift analysis...
... the expression of miR-216 and miR-217 and the lack of expression of miR-133a were characteristic of pancreatic tissue [36] Page of 17 (page number not for citation purposes) Journal of Translational ... down-regulation of miR-133a and miR-1 is associated with hypertrophic myocardium and skeletal muscle [33, 37-39] The suppression of miR- 133 has been shown to induce cardiac hypertrophy [37] miR-133a down-regulation ... healthy subjects The results ofanalysis by qRT-PCR and microRNA expression profiling were similar Page 12of 17 (page number not for citation purposes) Journal of Translational Medicine 2009,...
... Number of patients c.235delC c.235delC c.235delC c.235delC c.560_605ins46 c.299_300delAT c.176_191del16 c.223C>T Frameshift Frameshift Frameshift Frameshift Frameshift Frameshift Frameshift R75W ... 112: 329 -333 Yu F, Han DY, Dai P, Kang DY, Zhang X, Liu X, Zhu QW, Yuan YY, Sun Q, Xue DD, Li M, Liu J, Yuan HJ, Yang WY: Mutation of GJB2 gene in Chinese nonsyndromic hearing impairment patients: analysis ... of the populations are characteristic of northern and southern China, respectively This cohort of patients consisted of 158 males and 126 females from to 20 years old with an average age of12. 30...
... points Type of cluster All patients (n = 70) Adjuvant therapy (n = 39) Number of clusters Number of components Number of clusters Number of components A=B=C 261 212 A