... this binding depends on oppositely charged amino -acid residues within the transmembrane domain of each subunit Point mutations of these amino acids have revealed the importance of the arginine...
... inactivated by addition of mL 5% trichloroacetic acid and heated at 90 °C for 15 Radioactivity bound to protein was measured in hot-trichloroacetic acid- insoluble material as described previously ... from soluble rat brain extract For monitoring the tubulin isospecies, we used radioactive amino acids as well as specific antibodies After incorporation of [14C]nitrotyrosine or [14C]tyrosine into ... One of them, carboxypeptidase A, catalyzes sequential release of the ultimate C-terminal amino acid (except basic residues) from peptides and proteins The other, tubulin carboxypeptidase, participates...
... 23 Tsugita, A & Scheffler, J.J (1982) A rapid method for acid hydrolysis of protein with a mixture of trifluoroacetic acid and hydrochloric acid Eur J Biochem 124, 585–588 Ó FEBS 2004 N-Terminal ... was mixed with an equal volume of 2,5-dihydroxybenzoic acid (Aldrich; 10 mgÆmL)1 in 20% acetonitrile in water with 0.1% trifluoroacetic acid) , and the mixture was dried in the air Mass spectra ... was as follows The elution solvents were A (0.1% trifluoroacetic acid in water, pH 2.2) and B (acetonitrile with 0.1% trifluoroacetic acid) The linear gradient was 0–60% B in 60 and then 60–80% B...
... to” - “at the end of June” – vào cuối tháng “at the end (of something)” – vào lúc cuối, vào phần cuối (của kiện, thời gian) Ví dụ: at the end of the street (cuối đường), at the end of the book ... (am, is, are)” – chia dựa theo chủ ngữ “going to” viết tắt “gonna” văn nói Nếu muốn chia dạng phủ định, ta thêm “not” vào sau động từ “to be” - “She is going to” – cô Trong câu chủ ngữ “she” nên ... thể” - Cấu trúc “to be + going to + V –nguyên thể” – làm Cấu trúc dùng để đến kế hoạch , dự tính, định thông qua Hành động chắn xảy tương lai (thường gọi tương lai gần) Ta cần lưu ý động từ “to...