with the object naming service every string is mapped to an object
Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... peptides The list of light and heavy precursor masses was further used either to analyze the MS ⁄ MS spectra acquired in the data-dependent acquisition analysis or to build an inclusion list with the ... and msx-3d [12]) with a mass tolerance of p.p.m We therefore used MALDITOF-TOF and ⁄ or nanoLC-Orbitrap tandem MS to further identify peptides from this list Twenty-five mono-linked peptides and ... Ure2p, Ssa1p and Ure2p incubated with Ssa1p treated with BS2G are seen in lanes and 8, and 11 and and 13, respectively Similar samples treated with BS3 are seen in lanes and 9, and 12 and and 14,...
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fed up with the legal system, what's wrong and how to fix it 2nd (1994)
... legislators make the dozens of changes necessary to restore to all Americans their democratic right to understand their own laws and legal procedures Fed Up With the Legal System sets out many ... Americans with access to the law The harder it is for people to solve their legal problems on their own, lawyer groups seem to reason, the more business there is for lawyers If you doubt this, ... practiced in the civic courts, and promised suspension for any who took money with both hands or reviled an antagonist Page 11 #2 Abolish Probate THE PROBATE SYSTEM THE COURT-SUPERVISED PROCESS...
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Báo cáo y học: "Early down-regulation of the pro-inflammatory potential of monocytes is correlated to organ dysfunction in patients after severe multiple injury: a cohort study" pptx
... resuspended in isotonic solution (Isoton II®, Beckman-Coulter, Krefeld, Germany) and analyzed on a flow cytometer immediately Multiparameter flow cytometric analysis For flow cytometric analysis an Epics™ ... stimulation with LPS (Figures and 2) Statistics The statistical spread of the means is principally given as the standard error of the mean Data are plotted as boxes (interquartile range that contains the ... SZ, and PB contributed to study design, data collection and analysis, and drafted the manuscript WEM, EF, and MJ contributed to study design and manuscript review Acknowledgements We thank Viktoria...
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two-word phrasal verbs with the particle in that require into when used with an object
... car What did the rescue worker do? Alex is removing his toy train from the closet What is Alex doing to his toy train? 10 Carlos is standing on a table so that he can get the toy airplane that he ... But there is a difference: get emphasizes the change in location; come, go, and so on, emphasize the movement from one location to another It is very common to use the adverbs right and back with ... allow them to enter by giving them permission to enter or by opening a door, gate, and so on When the guests arrived, the butler opened the door to let them in The guard wouldn't let me into the...
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Tài liệu Module 7: Working with the Microsoft Outlook 2000 Object Model pptx
... method to give focus to an Explorer object; and use the IsPaneVisible and ShowPane methods to hide and display panes in an Explorer Activate method IsPaneVisible and ShowPane methods Customizing the ... display the pane or False to hide the pane The following example shows you how to use the IsPaneVisible method with the ShowPane method to hide and display panes in an Explorer 'Flip the settings ... passes the name of the view the user it trying to change to as well as the Boolean variable Cancel To cancel the view change and maintain the user’s current view, set the Cancel variable to True The...
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Tài liệu In this lab, 2 ISDN routers are required. If ISDN routers are not available, review the lab to become familiar with the process. An Adtran Atlas550 ISDN emulator is used to simulate the switch/ISDN cloud. pdf
... Please refer to the chart at the end of the lab to correctly identify the interface identifiers to be used based on the equipment in the lab The configuration output used in this lab is produced ... following according to the chart: • The hostname • The console • The virtual terminal • The enable passwords If there is a problem completing this, refer to the Network Address Translation (NAT) ... that the interface is spoofing This provides a mechanism for the interface to simulate an active state for internal processes, such as routing, on the router The show interface command can also...
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Tài liệu Working with the ADO Recordset Object pdf
... You can run this example by clicking on the button labeled Open a Recordset with GetString Display on the main form Figure A.5 shows what you will see Figure A.5 The GetString method is handy ... GetString method to stash the contents to the results text box The routine then saves that recordset using the Save method, and passes the adPersistADTG format enumerator You could save the recordset ... adPersistXML The code opens the file into a recordset and prints it by saving it to the results TextBox control Listing A.6 basRecordsetExamples.vb: Persisting a Recordset to Disk Sub PersistingARecordset(ByVal...
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Tài liệu Locate Records with the DataTable Object docx
... this at the form level, the connection that is used for the data adapter is not going to be left open the whole time the form is When the data table is filled, the connection is opened Then the ... Open and run the VB.NET-Chapter solution From the main form, click on the command button with the caption How -To 3.3 When the form loads, pick a new customer from the list that is presented in the ... the last How -To to load a ListBox control, this code sets modaCust to a SQL String and the connection string to be used mdtCust is then filled using the Fill method of modaCust Next, the first element...
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Tài liệu Work with the XML Document Object Model pdf
... If the data table has not been saved to the Session object, then it is created from scratch by first creating the data columns and then adding them to the data table The DataTable object is then ... saved to the Session object with the name MyDataTable If the Session object entry already exists, it is reassigned to the module variable mdtData Last, the data table is bound to the DataGrid object ... 12.3: Working with the XML Document Object Model As with How -To 12.1, when the page loads, you can enter a few names Enter the last and first names, and then click the button labeled Add to DataTable...
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Tài liệu The Internal Revenue Service Is Not Adequately Protecting Taxpayer Data on Laptop Computers and Other Portable Electronic Media Devices docx
... administration policies The Office of Management and Budget’s role is to help improve administrative management, to develop better performance measures and coordinating mechanisms, and to reduce any unnecessary ... testimony, and proposed legislation are consistent with the President’s budget and administration policies The Office of Management and Budget’s role is to help improve administrative management, to ... agreed with this recommendation The Mission Assurance and Security Services organization has refined the incident handling and reporting procedures to ensure sufficient details are gathered and...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... HO-1 and HO-2 in various human cell lines (A) Total RNA and proteins were prepared from the indicated cell lines and subjected to northern blot analysis and (B) western blot analysis (A) Northern ... blot analysis Each lane contains 15 lg total RNA The bottom panel shows the expression of 18S rRNA as an internal control Note that the blot was exposed to the film for the longest time (1 min) to ... (A and B) and HepG2 cells (C and D) were left untreated or treated with SA (5 mM) for the indicated time and harvested The upper panels in (A) and (C) show the northern blot analysis of HO-1 and...
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Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt
... signal is missing The human phospholipases D1 and D2 are mainly associated with the plasma membrane or with the membranes of intracellular organelles although they lack a transmembrane domain They ... immunization is highly conserved from human to mouse and rat and the antiserum recognized Hu-K4 in human, rat and mouse brain (not shown) To identify the subcellular distribution of Hu-K4 we analysed transiently ... transiently transfected with the Hu-K4 cDNA and stained with DAPI (4¢,6-diamidino-2-phenylindole; A,D), with anti-Hu-K4 and anti-rabbit Cy2 (C,D), or with anti-(protein disulfide isomerase) and...
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Báo cáo khoa học: N-Methylation in polylegionaminic acid is associated with the phase-variable epitope of Legionella pneumophila serogroup 1 lipopolysaccharide pptx
... only to designate their positions relative to N2 and not refer to stereoisomerism) The lower-®eld H NMR signal of belonged to the N-methyl group at N2 in the E isomer and the higher-®eld signal to ... 5-N-acetimidoyl-7-N-acetyl-5-N-methyllegionaminic acid (3-E and 3-Z) belonged to the N-methyl groups at N1 in trans and cis orientation to N2 of the acetamidine group, respectively (Fig 8; the descriptors cis and trans for the two N-methyl ... 2625 epitope and to correlate it to virulence For this purpose, the isogenic mutant 5215 was generated by deletion of the LPS biosynthesis operon ORF 8±12 in the virulent wild-type RC1 With the aid...
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Báo cáo khoa học: The N-terminal cysteine pair of yeast sulfhydryl oxidase Erv1p is essential for in vivo activity and interacts with the primary redox centre doc
... colour, due to the bound FAD (Fig 5) The two most striking changes upon cysteine exchange are the black appearance of the C30S protein and the orange colour of the C130S mutant Changing the other cysteine ... with a His6-antibody and chemiluminescence with the exception of C133S In comparison to the wildtype protein the C30S, C33S and to a lesser extend the C130S mutant proteins are predominantly found ... using tetrad analysis of an insertion mutant of the ERV1 gene (Table 2) The analysis is complicated by recombination events due to the position of ERV1 close to the centromere, but the results...
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Cancer Research UK’s strategy 2009–2014: Cancer Research UK’s aim is to reduce the number of deaths from cancer. Our future plans are ambitious, but they are in line with the challenge and the responsibility we face. docx
... damage leads to cancer, mechanisms of cellular DNA repair, immune system and inflammatory responses to cancer, invasion and metastasis and genetic pre-disposition to cancer Scientists at Cancer Research ... organisations to understand what causes delay in diagnosis, establish the best ways of helping the public to recognise symptoms and to get them checked out by a doctor, and to understand the link between ... knowledge and understanding of the disease that research provides and in which Cancer Research UK is leading, both in this country and internationally There is clearly much more we need to Cancer is...
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Báo cáo khoa học: Association of RNA with the uracil-DNA-degrading factor has major conformational effects and is potentially involved in protein folding pot
... insert panel) MS analysis of the two protein bands further conrmed the lack of any detectable post-translational modications (data not shown) In the rst fraction, UDE eluted together with a large ... fractions by tryptophan uorescence (Fig 3A) and by CD spectroscopy (Fig 3B) In the case of tryptophan uorescence, we monitored the specic local milieu of the four tryptophans within the sequence ... RNAUDE is able to slowly transform into the conformation characteristic for UDE, we suggest that the origin of RNAUDE complex formation is within the translation-coupled folding mechanism, which...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... Arabidopsis thaliana [12] The protein is composed of 225 amino acid residues and is rich in Glu and Lys The protein has no transmembrane domain, but is associated with the plasma membrane, and was ... 2) The aim of this study was to clarify the mechanism of the specific tight association of PCaP1 with the plasma membrane in vivo and in vitro Almost all PCaP1 was associated with the membrane and ... PtdIns(3,4,5)P3 in the membrane N-myristoylation anchors PCaP1, and the interaction with PtdInsPs may contribute to the stability of the attachment of PCaP1 to the membrane It has been shown that the attachment...
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Báo cáo khoa học: The unique pharmacology of the scorpion a-like toxin Lqh3 is associated with its flexible C-tail pdf
... residues of the loop preceding the a-helix, the loop connecting the second and the third b-strands, and the C-tail (Fig 2A) decrease in the dissociation rate constant at lower pH [18] To clarify the ... formation and breakdown of hydrogen bonds and changes in the tilt and twist angles of the backbone, and should be sensitive to the pH of the medium As His residues contribute in part the hydrogen ... as the slower toxin dissociation rate at low pH [18], we speculate that upon toxin binding to the channel, acidic pH favors toxin conformation with high afnity for the receptor, and reduces the...
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Báo cáo khoa học: Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern pot
... Taken together, these data demonstrate that the FLAG epitope does not interfere with the cGMP responses of GC-A to ANP or the membrane localization, and therefore represents a good tool to facilitate ... expressing the FLAGtagged GC-A receptor The cells were fractionated and the membrane fraction was used to enrich the receptor by immunoprecipitation with anti-FLAG IgG Western blot analyses with antibodies ... ice to separate the cytosol according to the manufacturer’s instructions (CF-Cyt, NanoTools, Teningen, Germany) After centrifugation at 1000 g for at °C, the supernatant containing the cytosolic...
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