... among BNP categories in either males or females. In a commu-nity-based sample, higher plasma BNP levels were found to be associated with < /b> an increased risk of BP progression in males, but not ... There were no significant differences in SBP and DBP levels, or in the pulse of subjects with < /b> or without the 16 repeat allele (Table 3). The plasma BNP level was significantly higher in the ... them had the 16 repeat allele. All subjects were classified into 3 groups based on their BMI levels (lean, BMI<18.5; normal, 18.5<BMI<25; obese, BMI >25). There was no associa-Int....
... N-terminal ubiquitin (Nub) and C-terminal ubiquitin (Cub) but the Nub and Cub have high affinity towards each other and is able to reassemble back together spontaneously into ubiquitin protein. ... cellular proteins interacting not only with < /b> soluble HBV proteins, but also with < /b> membrane bound HBsAg. To this end, a linearised form of HBV genome has been constructed in the mammalian expression ... known about cellular proteins that interact with < /b> HBsAg and thereby contributing to HBV morphogenesis. Using the yeast split-ubiquitin system, a number of cellular membrane proteins have been...
... plan with < /b> adapting activities for part B Speaking unit 10 English 11 Unit 10: Nature in danger Part B: Speaking I/Objectives: At the end of this lesson students will be able to talk about ... about the reasons why nature is in danger and suggest possible measures to protect it Pre teaching vocabulary -fur(n) [fə:] lông -hunt(v) [hʌnt] săn b n -capture(v) ['kæpt∫ə] b t ... up and lead in: ask about date ? weather ? absence? What’s the date today?What’s the weather like today? Who is absent today? Show the picture of a fire from text book (using power point)Question?...
... are drawn as balls-and-sticks, with < /b> the DHAPphosphorous in orange. The Zn2+coordinative bonds are indicatedby dotted lines, together with < /b> the hydrogen bonds established byDHAP with < /b> the metal-coordinating ... catalysis, not only because it con-tributes to Zn2+coordination but also because of itsdirect involvement in substrate binding (Fig. 3A ,B) .Indeed, as detailed above, Asp291 stabilizes DHAPthrough ... number of stabilizing interac-tions with < /b> the protein milieu by contacting both pro-tein residues and solvent molecules. In particular, theDHAP phosphate moiety establishes a salt bridge with< /b> Arg47...
... kDa, are labelled with < /b> stars.Ó FEBS 2003 BAP31 associates with < /b> c-actin and myosin B (Eur. J. Biochem. 270) 345containing p20–Flag, which contains the membrane-span-ning domain of BAP31 but not ... associated with < /b> membrane-bound immuno-globulin in lysates of B lymphocytes [6]. Consistent with < /b> thisproposed role, BAP31 can form transient associations with< /b> newly synthesized IgD and cellubrevin ... against a subset of the NCBI GenBank proteindatabase (http://www.ncbi.nlm.nih.gov; nonredundant pro-tein database release July 2, 2001) filtered with < /b> the wordÔhumanÕ (resulting in a database of...
... : 10 enzyme/BLG ratio. NativeBLG, circles. A rabbit anti-BLG Ig was used.Ó FEBS 2002 Proteolysis of heat-unfolded b- lactoglobulin (Eur. J. Biochem. 269) 1369bulin to h ydrolysis by gastric ... captureELISA by u sing BLG specific mon oclonal or polyclonalantibodies as capture a ntibodies. A ll steps were c arriedout at 30 °C. Polyclonal rabbit a nti-BLG Ig was diluted4000–10000 times in carbonate ... plates were incubated with < /b> a fixedconcentration of biotinylated BLG (10–200 ngÆmL)1,depending on the antibody). After i ncubation and anotherfour washes, plates were incubated with < /b> horseradishperoxidase-labeled...
... most frequently detected bifidobacteriaspecies are Bifidobacterium breve, Bifidobacterium infan-tis, Bifidobacterium bifidum and Bifidobacterium longum.The latter one also inhabits the intestines of ... hydrogen bonds with < /b> numbering of residues interacting with< /b> fructose (the hydrogen bond lengths are in Table 2).Crystal structure of B. longum b- fructofuranosidase A. Bujacz et al.1734 FEBS Journal ... interface between both b- sheets is formed by thepredominately hydrophobic residues. The two domainsare connected by a hinge region with < /b> a two-turn helix.Interactions between the b- propeller and b- sandwichdomains...
... MINT-7546830: Beta-conglycinin (uniprotkb:P25974) binds(MI:0407)toAlpha subunit of BC (uniprotkb:P13916)bypull down (MI:0096)lMINT-7547140, MINT-7547249: Beta-conglycinin (uniprotkb:P25974) binds ... intended toinhibit gastric and pancreatic lipases [16] but it hasbeen found to inhibit FAS by interacting with < /b> its TEdomain; however, it has shown poor systemic stabilityand bioavailability [17]. ... or basic (bluering). Arrows indicate hydrogen bonds toside chain (green) and backbone (blue)residues.C. Martinez-Villaluenga et al. b- conglycinin peptides inhibit fatty acid synthaseFEBS...
... Several species absorb strongly inthe UV -B range [1,2,4]. Lichens and their metabolites havebeen demonstrated to possess numerous biological activit-ies, including: antiviral, antibacterial, antitumor, ... saline(Biological Industries, Beit-Haemek, Israel), washed with< /b> NaCl/Piand counted in 0.2% trypan blue solution(Biological Industries). Viability was calculated as theaverage percent of trypan blue ... are fungal meta-bolites with < /b> UV absorption at 310–320 nm, and can beconsidered to be Schiff bases (enamino ketones), whichpossess a common cyclohexenone ring system linked by anaminoacidoranamino-alcohol[11].Incontrast,MAAsareUV...
... enzyme–substrate complexes had anA-unit bound in subsite )2. Upon more extensivehydrolysis, presumably leading to depletion of bindingA. Sørbotten et al. Degradation of chitosans with < /b> ChiBFEBS ... showthat nonproductive binding events are not necessarily followed by substraterelease but rather by consecutive relocations of the sugar chain.AbbreviationsChiB, chitinase B; DP, degree of polymerization; ... oligomers with < /b> decreasing FA.This was expected because the average chain length ofD-blocks that will not be attacked by the chitinaseincreases with < /b> decreasing FA(Table 2). By extensiveChiB-degradation...
... interaction of stefin B with < /b> various combinations of phospholipid monolayersand bilayers. Interaction of stefin B in the prefibrillaraggregated state with < /b> model lipid membranes wasprobed using the calcein ... composed of loosely bound oligomericblocks can be seen and, at pH 3.3 (Fig. 1B) , necklace-like structures built from basic ellipsoid blocks (similarto protofibrils) are observed. At pH 7.3, ... stefin B induce mem-brane leakage by forming channels. The preference foracidic lipids suggests that the membrane might bedestabilized simply by surface interactions. However,the permeabilization...
... membrane insertion of b- amyloid peptide (Ab1–40),which may potentially inhibit the fibril formation. J. Biol. Chem.277, 6273–6279.41. Wojtowicz, W.M., Farzan, M., Joyal, J.L., Carter, K., Babcock, ... neurotoxic.Alternatively, APP can be hydrolyzed by the b secretaseactivity at the N-terminus of Ab, which is successivelyreleased by the c secretase after cleavage within themembrane [6] either between residues ... E-mail: picone@chemistry.unina.itAbbreviations: AD, Alzheimer disease; Ab, amyloid beta-peptides;Ab-(1–40), amyloid beta-peptide 1–40; Ab-(1–42), amyloid beta-peptide 1–42; APP, amyloid precursor...
... substrate, asalso found in other studies [12,13].Activity of Bga1 on polymeric substratesThe ability of Bga1 to hydrolyse b- galactosidic bondsin polymeric substrates was tested with < /b> two b- 1,4-galactans ... (incubation: 1 h at 30 °C).The amount of NADH (eNADH¼ 6300 LÆmol)1Æcm)1) pro-duced was determined by measuring the absorbance at340 nm against a blank (incubation mixture without sub-strate). ... molecular mass of the b- galactosidase was deter-mined by gel-permeation chromatography using a 16 ⁄ 70column filled with < /b> Bio-Gel A-1.5 m (Bio-Rad), equilibrated with < /b> 0.1 m acetate buffer pH 5 containing...