weapons of our warfare next generation techniquesand tools for detection identification and analysis
Báo cáo y học: "Improved variant discovery through local re-alignment of short-read next-generation sequencing data using SRMA" pot
... within SAMtools [10,20] In Figure 1, we plot receiver operator characteristic (ROC) curves for the detection of the known SNPs, deletions, and insertions For all types of variants, performing local ... variants and to reduce false positive variant detection Page of 12 decoded base sequence and base qualities produced by BWA were used by SRMA The aligned reads were used for variant calling before and ... produced with BFAST of a human cancer cell line (U87MG; SRA009912.1) Variants were called with SAMtools before and after application of SRMA (see Materials and methods) Deletions and insertions (indels)...
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Báo cáo y học: " Evaluation of next generation sequencing platforms for population targeted sequencing studies" pot
... quality, we next performed a fourway comparison of the base calls generated from the three NGS technologies and ABI Sanger The identification of heterozygous and homozygous alternate loci was performed ... DNA amplification and manipulation and is useful for the detection of indels and structural variants Therefore, as opposed to the library construction processes for Roche-454 and Illumina GA, ... sequencing analysis; SL, and TS performed Sanger sequencing analysis; XW, and KB performed next gen sequencing experiments; KF, ET, NS, RS, SL, and SM facilitated the study; KF, SL, PN, and OH designed...
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báo cáo khoa học: " Integration of molecular biology tools for identifying promoters and genes abundantly expressed in flowers of Oncidium Gower Ramsey" pps
... nm/band-pass 465-510 nm for CFP, 488 nm/bandpass 500-530 nm for GFP, 514 nm/ bandpass 525-555 nm for YFP, 561 nm/bandpass 575-630 nm for RFP and mCherry, and 488 nm/band-pass 650-710 nm for chloroplast ... patterns for both OnTI1 and OnTI2 promoters were similar Staining was observed at the top of the styles and at the junction of the pedicel and flowers (Figure 7) Sequences from next generation ... wavelengths and Hsu et al BMC Plant Biology 2011, 11:60 http://www.biomedcentral.com/1471-2229/11/60 Page 12 of 14 Figure Strategy for identification of BAC clones containing genes of interest Genes of...
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Báo cáo y học: "Next-generation biomass feedstocks for biofuel production" pptx
... acres) of forestland that the US Forest Service classifies as incapable of growing cubic meter per hectare annually and is not considered as a viable biofuel feedstock growth area [9] Current forest ... these lands specifically for biofuel production are hybrid poplar, eucalyptus, loblolly pine, willow and silver maple One hypothetical distribution of the forestry resources as a function of geography ... achieved over this time period, of 247 billion liters (65 billion gallons) per year Forestry resources A recent report [9] reported that the amount of forestland, as of 2002, in the United States...
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Tools for management, workshop and consulting
... Elisabeth and Erwin Andler, for their love, trust, support and never-ending faith in me Tools for Project Management, Workshops and Consulting A must-have compendium of essential tools and techniques ... a structure for your thoughts and actions The tools NOT replace your own intuition, lines of thought, and critical dialogue with the topic Use tools as a stimulus and suggestion and customise ... evaluate and receive feedback 45 Problem solving approach and application Example of a ‘shopping list’ of tools for a workshop Problem solving process steps: Categories of tools: Tools and techniques:...
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Tài liệu 50 Software Tools for Speech Research and Development doc
... values of processing parameters, and the names and headers of all source files The header is a recursive structure, so that the headers of the source files themselves contain the names and headers of ... one of CPU speed 50.9.5 Source Availability It is unfortunate but true that the best documentation for a given speech processing command is often the source code Thus, the availability of source ... Cross-Platform Compatibility If your hardware platform may change or your site has a variety of platforms, then it is important to consider whether the speech software is available across a variety of...
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Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx
... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII ... N-terminus of the protein was also shown to be required for the activity and/ or secretion of the GCPII carboxypeptidase As for the N-terminally modified variants, the absence of the intracellular and ... final volume of 225 lL A 25 lL mixture of 950 nM ÔcoldÕ NAAG (Sigma) and 50 nM H-labelled NAAG (51.9 CiÆmmol)1; New England Nuclear) was added to each tube and incubation continued for 20 The reaction...
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Báo cáo khoa học: Sequences downstream of the transcription initiation site are important for proper initiation and regulation of mouse ribonucleotide reductase R2 gene transcription ppt
... reductase is composed of two subunits, proteins R1 and R2 Both subunits are required for enzyme activity and it could be reasonable to assume that the expression of the R1 and R2 mRNAs should be ... with high concentrations (50 lM) of polyamide (Fig 1C) Importance of the R2 TATA-box and TBP binding to the R2 promoter We next studied the importance of TBP and the R2 TATA-box in an in vitro ... identified as important for expression from different promoters, for example downstream promoter element (DPE) [14] We therefore analyzed the sequence of the R2 promoter and the 5¢-UTR, and compared it...
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Risk of cancer from diagnostic X-rays: estimates for the UK and 14 other countries docx
... 3, 10, 12, and for males, and 1, 4, 11, 17, and for females ‡Number of cases in body of table rounded and not always, therefore, add up to the totals given Table 4: Estimated number of radiation-induced ... estimation of radiationinduced risk UK cancer incidence and all-cause mortality rates We used cancer incidence rates for England and Wales (1988–92) for male and female individuals in 5-year age bands.16 ... used the UK age and sex distributions of diagnostic X-rays and organ-specific radiation dose estimates throughout For the USA, only the frequencies of CT scans and of all types of X-ray examinations...
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Báo cáo khoa học: "Finding Ideographic Representations of Japanese Names Written in Latin Script via Language Identification and Corpus Validation" docx
... Table 1: Accuracy of language origin identification for names in the training set (JAP, CHI, and ENG stand for Japanese, Chinese, and English, respectively) Language Identification of Names Given ... Computer and the Humanities, 35( 4): 389–420 K S Jeong, Sung-Hyon Myaeng, J S Lee, and K S Choi 1999 Automatic identification and back-transliteration of foreign words for information retrieval Information ... (over (1)) Evaluation Results and Analysis Effectiveness of Corpus Validation Table and Table present the precision, recall, and F statistics for the gold standards GS-GN and 11 http://mirrors.nihongo.org/monash/...
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Báo cáo khoa học: "An Online System for Corpus Management and Analysis in Support of Computing in the Humanities" pot
... Application, resources and its format In Angelika Storrer, Alexander Geyken, Alexander Siebert, and Kay-Michael W¨ rzner, u editors, Proceedings of KONVENS 2008 — Erg¨ nzungsband Textressourcen und ... Birgit Hamp and Helmut Feldweg 1997 Germanet - a lexical-semantic net for german In In Proceedings of ACL workshop Automatic Information Extraction and Building of Lexical Semantic Resources for NLP ... the core of the desktop It allows to upload and download documents as well as sharing them with other users and groups It follows the look and feel of the Windows Explorer Documents and repositories...
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Báo cáo hóa học: " Detection of epithelial apoptosis in pelvic ileal pouches for ulcerative colitis and familial adenomatous polyposis" pot
... ileal pouches for ulcerative colitis and familial adenomatous polyposis Journal of Translational Medicine 2010 8:11 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... Rockford) All numerical results are expressed as the mean ± SEM of the indicated number of experiments The results of blots are presented as direct comparisons of bands in autoradiographs and ... immunoblotting, immunoprecipitation assays and histological analysis to determine the expression of pro-apoptotic and anti-apoptotic proteins, and detection of apoptosis by Annexin V fluorescence...
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Báo cáo sinh học: " CODEHOP-mediated PCR – A powerful technique for the identification and characterization of viral genomes" pptx
... review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented While the focus of this study was ... obtained for comprehensive virus phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding ... Page 23 of 24 (page number not for citation purposes) Virology Journal 2005, 2:20 HOP PCR strategy, Jorja and Steve Henikoff, of the Fred Hutchinson Cancer Research Center, for the creation and maintenance...
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báo cáo hóa học:" The HIV-1 Non-subtype B Workgroup: An International Collaboration for the Collection and Analysis of HIV-1 Non-subtype B Data" pot
... international workgroup for the collection and analysis of RT and protease sequences and data from persons infected with non-B HIV-1 subtypes Currently, the workgroup consists of investigators from ... differences in genotypic evolution: analysis of subtypes B and C reverse transcriptase and protease sequences Program and abstracts of the 11th Conference on Retroviruses and Opportunistic Infections; ... Robertson DL, Anderson JP, Bradac JA, et al.: HIV-1 Nomenclature Proposal: A Reference Guide to HIV-1 Classification Human Retroviruses and AIDS: A Compilation and Analysis of Nucleic and Amino Acid...
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Báo cáo hóa học: " Research Article A New Hybrid Algorithm for a System of Mixed Equilibrium Problems, Fixed Point Problems for Nonexpansive Semigroup, and Variational Inclusion Problem" ppt
... iterative algorithm for finding a common element of the set of solution for a system of equilibrium problems and the set of common fixed points for a finite family of nonexpansive mappings and a nonexpansive ... method of finding the common element of the set of common fixed points for a finite family of a ξ-strict pseudocontraction, the set of solutions of a systems of equilibrium problems and the set of solutions ... method for finding a common element of the set of solutions of generalized mixed equilibrium problems, the set of fixed points of a finite family of quasinonexpansive mappings, and the set of solutions...
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