... analyzed The analysis ofthe predicted structure suggested that the presence ofthe shorter lateral chain of Val311 instead of Leu allows forthe movement of Tyr 239 and Glu309 towards the guanine ring, ... 155 168 158 208 802 7 .3 6.5 5.8 7.9 7.4 1.2 280 37 1 562 774 1411 36 2 ± ± ± ± ± ± 20 13 23 40 12b 106a GTP Wild-type Y 239 F V311L E309Q E309M Y 239 F ⁄ E309M 108 167 115 30 0 169 34 8 Vmf ⁄ Km (LÆmin)1Æmg ... that the amino group at carbon ofthe guanine ring may interact with the side chain of Glu309, whereas the carbonyl group at position ofthe guanine ring may interact with the hydroxyl group of the...
... may be their countenance was not more than necessary The purity of his style, the delicacy of his turns, and the justness of his characters, were all of them beauties which the greater part of his ... in the formation of his characters from the observations of Theophrastus, of whom he was a disciple; and Theophrastus, it is known, was not only the disciple, but the immediate successor of Aristotle, ... and therefore not proper forthe stage) as through an affected wit: a wit which, at the same time that it is affected, is also false As there is some difficulty in the formation of a character of...
... Biosystem) and the T3 Thermocycler (Biometra) Samples were preincubated at 95 °C for 10 The PCR cycle consisted of 30 sec of denaturation at 94 °C, 30 sec of annealing at 50 °C, and of extension ... by using the LightCycler software 3. 5 Quantification by FISH was performed with a Cy3-labeled PAOMIX (PAO462, PAO651 and PAO846) and an FITC-labeled EUBMIX (EUB 338 , EUB 338 II and EUB 338 III) probe ... values forthe DNA standards versus logarithm of their starting copy number was constructed A typical linear regression line is shown in Figure The averages of R2 value and the slope ofthe calibration...
... about the difficulty ofthe test 31 Chart 5: Students’ comments on the difficulty ofthe test When being asked about the difficulty ofthe test, 23% ofthe students share the same idea that the ... that the spread of scores or score distribution and the range of ability are not very wide On the other hand, the type ofthe test is achievement which is to measure the extent of learning ofthe ... from the easiest to the most difficult ones and the test items, therefore, may cover the content ofthe course book Or in other words, the test has content validity Besides the mean, the mode, the...
... worse: the vast majority of economists never even bother to ask the question ofthe proper scale ofthe economy relative to the environment In standard economic theory the economy can simply grow forever, ... hand over the land, they wanted the government to give them control ofthe land but not responsibility for it, and they wanted an increase in the fat subsidies they enjoyed for grazing their sheep ... management plan forthe forests within the range ofthe northern spotted owl that would meet the legal requirements ofthe Endangered Species Act, while also recognizing the needs of communities...
... Receiving the equivalent of 15 mg/day or more of prednisone for at least one month o Have a history of organ transplant • Have signs or symptoms of active TB, regardless ofthe size of induration ofthe ... Evaluation for Treatment (LTBI)” to the TB Control Program ofthe local health department The referral for evaluation for treatment of LTBI is recommended, not required Part of Form I-6 93 should ... Referral for treatment and/or completion of treatment for LTBI is not required forthe civil surgeon to sign the I-6 93 form As regards TB, the signature indicates the applicant is free of Class...
... part is unique for GIRK1 among the Kir3.x isoforms Further truncations ofthe C-terminus revealed a 49 amino acid stretch (amino acids 36 2–411) that was the most prominent target for phosphorylation ... [18] To assess the role ofthe S ⁄ Ts in the regulation of GIRK1 via PKA in a manner that is unbiased by the eventual contributions ofthe other subunits in a heterooligomer, the corresponding ... with the KHA2 antisense oligonucleotide (25 ng per oocyte) together with the appropriate RNA (amounts in pg per oocyte): m2R: 1500; GIRK1F 137 S: 37 .5; GIRK1F 137 SS385C: 37 .5; GIRK1F 137 SS401C: 37 .5;...
... mixture ofthe sine curve of analogue and 0/1 values of digital It also refers to the integration of AV and IT Idei envisioned the V AIO as a platform for a network service over which Sony could offer ... however; these properties are not themselves the success factors Rosenzweig calls the tendency to observe the results first and then rationalize the company’s strategies the Halo Effect.14 The real ... nowhere? The media have speculated about why the fortunes of these two companies changed so rapidly, but their evaluations are usually superficial and focus only on short-term performance Often,...
... deficiencies in the Department’s operations and the need for corrective action The Office ofthe Deputy Inspector General for Auditing is one of six components within the Office ofthe Inspector ... difference in the lives ofthe warfighter Core Values • Integrity • Efficiency • Accountability • Excellence Office ofthe Deputy Inspector General for Auditing FY 20 13 Audit Plan The Office ofthe Deputy ... from the fund, any other funds expended, and any unobligated funds remaining in the fund as of September 30 , 20 13 Management ofthe Need for Cash Objective: Review the adequacy of DoD policies for...
... friendly with Piggy,and the two of them refuse to adopt the new, less structured wayof life that most the boyson the island experience Both of them are very firm in their belief of organization andcivilization, ... on the island breaksdown Jack and his hunters form a tribe of savage boys on the far side ofthe island Most ofthe boys follow Jack because they relish the idea of meat and fun but despise theidea ... red-headed boy He appears in thenovel as the leader ofthe boys' choir During the first blowing ofthe conch and the firstassembly, Jack loses the election for chief He and Ralph, the protagonist, initially...
... Studies ofthe biology of cancer have informed the biology of aging and vice versa A deeper understanding ofthe biology of aging has the potential to affect not just cancer, but all ofthe disorders ... prevention of disease was the subject of breakthroughs in all three ofthe medically focused panels These breakthroughs include the prevention of cardiovascular disease, the prevention of a variety of ... one ofthe most useful features ofthe FEM to the Office ofthe Actuary, namely the ability to model the potential effects on future costs of a variety of hypothetical or likely trends in medical...
... systematic effort has hitherto been made forthe exploration ofthe entire group Under the direction ofthe Dominion Government, the waters and shores ofthe north and east coast ofthe islands ... by the sinking ofthe earth’s surface, and the inflowing ofthe waters ofthe ocean, forming the most remarkable labyrinth of inlets, sounds, straits, channels and passages on the face ofthe ... the 5th of May, having secured the services of two Hyda Indians, one a native of Ninstints, the extreme southern village ofthe Hyda nation, familiar with the shores ofthe southern portion of...
... interface has to be designed The research area therefore consists of two major parts, namely the design ofthe underlying system, and the design ofthe user interface. For future reference, the underlying system will be called 'system concept', the user interface will be ... This concludes the user analysis. It's been investigated who the future users ofthe system will be, and what their needs and requirements regarding ADAS are. The list of requirements can assist with the further definition ofthe system The following paragraph discusses the possibilities ofthe system concept, investigating which requirements can be ... Law may prevent ADAS from operating optimally The resulting roadmap is based on the findings from chapter 1 and 2 as well as other ADAS road maps36, 37 . The use of literature should increase the feasibility ofthe roadmap. Still, the following assumptions have been used during the ...
... systematic effort has hitherto been made forthe exploration ofthe entire group Under the direction ofthe Dominion Government, the waters and shores ofthe north and east coast ofthe islands ... by the sinking ofthe earth’s surface, and the inflowing ofthe waters ofthe ocean, forming the most remarkable labyrinth of inlets, sounds, straits, channels and passages on the face ofthe ... the 5th of May, having secured the services of two Hyda Indians, one a native of Ninstints, the extreme southern village ofthe Hyda nation, familiar with the shores ofthe southern portion of...
... sequence (Lys306 to Glu320) and a His box-like sequence (Tyr3 43 to Tyr362), in the central region of NUB1 (see below) The Cys box and His box are commonly found in sequences of DUBs [33 ] DUBs are ... whether the immunoprecipitates of FLAG-NUB1 have the enzymatic activity of UCH As shown in Fig 3B (upper panel), we incubated GST-UbC1 with the immunoprecipitates of FLAG-NUB1 for (lane 3) , 30 ... that of NUB1 with the exception ofthe UBA2 insertion, we hypothesized that the immunoprecipitates of NUB1L had the same enzymatic activity as those of NUB1 To prove this, we performed the in...
... introduced the syntactic and shallow semantic structures and discussed their im12 Systems ROUGE TF*IDF 0 .35 9458 SYN 0 .36 9677 SEM 0 .38 9865 SYNSEM 0 .37 6126 ROUGE L ROUGE W ROUGE SU 0 .33 4882 0 .33 66 73 0 .35 6792 ... 0 .35 6792 0 .34 033 0 0.124226 0.126890 0. 132 378 0.126894 0. 130 6 03 0.129109 0.145859 0. 135 901 Table 1: ROUGE F-scores for different systems pacts in measuring the similarity between the sentences in the ... cases where the query is composed of two or more sentences, we compute the similarity between the document sentence (s) and each ofthe query-sentences (qi ) then we take the average ofthe scores...
... N-glycan-processing pathway in molluscs We thank the Core Facilities for Proteomic research at the Academia Sinica, Taiwan, forthe use ofthe MALDI-QTOF instrument This work was supported by Grant-in-Aid for Scientific ... cleavage at the chitobiose core firmly shows the existence ofthe extra Gal residue on the reducing end GlcNAc A fragment ion at m/z 433 provides direct evidence of a Gal-Fuc unit whereas the ion ... as 4-linked Fuc on the basis ofthe EI-MS pattern (Fig 4B) Taken together, the results unambiguously establish that the extra b-Gal residue is 4-linked to the Fuc on the arm of a difucosylated...
... nuclear localization and can occur in the absence of stress Mol Cell Biol 13, 139 2–1407 [published errata appear in Mol Cell Biol (19 93) 13, 31 22 31 23 and 13, 38 38 38 39] Tanabe, M., Kawazoe, Y., Takeda, ... Determining the optimal binding sequence for HSF2 (Eur J Biochem 269) 2 533 Fig Comparison ofthe affinity ofthe in vivo and i.v.s HSF2 forthe oligonucleotides 2U519, Shval and Shvalspz 32 P-Radiolabeled ... extracts, forthe oligonucleotides CL39 and 39 woGAA and forthe oligonucleotides CL 83, 83woGAA and 83woTA 32 P-Radiolabeled oligonucleotides were incubated with F9 cells extracts and the HSF2–...