vpr s structure and nuclear localization function
Báo cáo y học: "Antiviral activity of a-helical stapled peptides designed from the HIV-1 capsid dimerization domain" pps
... peptides of such short length tend to exist as random structures despite the fact that the secondary and tertiary structures of this segment in the CTD protein are a-helical Since the a-helical structure ... gave a series of endothermic heat pulses (Figure 4) consistent with dissociation of protein oligomers modeled as dimers, with dimer dissociation constant (K diss ) ~15 μM and dissociation enthalpy ... Figure S1 ) penetrate cells Stapled peptides enhance dissociation of dimers Figure Sequences and primary structure of linear and hydrocarbon-stapled peptides, indicating the stapling sites nor...
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Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
... are possible in ve positions and three substitutions are possible in four positions) These positions include positions 10 and 64 Interestingly, the completely conserved positions and those exhibiting ... studies, ellipticity changes 4180 Acknowledgements We are grateful to Professor N V Joshi for the analysis of TIM sequences and several illuminating discussions The mass spectral facility was ... active site The availability of crystal structures of TIMs from 21 sources and the large database of TIM sequences from various sources facilitate an analysis of mutational effects Most importantly,...
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Tài liệu Báo cáo khoa học: Analysis of proteins and peptides on a chromatographic timescale by electron-transfer dissociation MS ppt
... Arg75, and the predicted mass of this peptide is thus 6073 Da The measured mass is 363 mass units higher than predicted Further analysis of c¢-type ions (singly and doubly ETD-MS analysis of peptides ... of peptides and proteins charged) indicates that the Tyr residues at positions 31 and 40 are both 80 mass units higher in mass than expected and are therefore phosphorylated Analysis of the z¢Æ-type ... Troyanskaya OG & Hunt DF (2007) Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry Proc Natl Acad Sci USA 104,...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
... Each family/cluster had 10–12 structures An average structure was also chosen from this family as representative structure Determination of peptide structures Conformational space was searched for ... with a sweep width of 5000 Hz ROE cross-peak volumes were measured using ROESY spectra with 300 ms spin-lock times and NOESY cross-peak volumes for hexapeptides were measured at 200 ms mixing ... mean square deviations (rmsd) between each possible pair of structures, and was divided into several conformational families The average structure was taken from each family and chosen as starting...
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Báo cáo khóa học: Antimicrobial activities of heparin-binding peptides pdf
... Sweden The purity and molecular mass of these peptides was confirmed by mass spectral analysis (MALDI.TOF Voyager) Microorganisms Enterococcus faecalis 2374, Escherichia coli 37.4, Pseudomonas ... dose– response characteristics of the RDA was used and the linear relationship between zone diameter and log10 concentration for LL-37 was determined by least mean squares regression analysis ... synthesized and tested in bactericidal assays using E faecalis as the test organism, and the results demonstrate that these peptides are also antibacterial A correlation between peptide length and antibacterial...
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Báo cáo Y học: Purification, crystallization, NMR spectroscopy and biochemical analyses of a-phycoerythrocyanin peptides pptx
... measurements of these crystals, as well as those of the crystals in this study, failed Despite cryo-conditions, the molecular order of the crystals decreased rapidly during measurements The reason ... radiationless energy dissipation However, the missing fluorescence of PEC in intact phycobilisomes and different adapted cells of M laminosus support this suggestion [4] ACKNOWLEDGEMENTS This work was ... is the enhanced accessibility of isolated subunits to proteolytic enzymes Nevertheless, other factors exist which are responsible for the degradation (see Results) The analyses of the peptides...
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Báo cáo Y học: Expression and distribution of penaeidin antimicrobial peptides are regulated by haemocyte reactions in microbial challenged shrimp pptx
... mRNAs were detected in circulating haemocytes in blood vessels and sinuses and in cells present within most tissues The shape of the positive cells suggests that they are infiltrating haemocytes ... penaeidin transcripts and proteins is restricted to haemocytes either circulating in blood vessels irrigating tissues such as the brain, hepatopancreas or gills, or infiltrating tissues such as subcuticular ... investigate the ability of tissues other than haemocytes to express penaeidins and to study the distribution of both transcripts and peptides in response to challenge, shrimp tissues were analysed...
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Báo cáo khoa học: Non-hydrolytic functions of acetylcholinesterase The significance of C-terminal peptides pptx
... Parkinson s disease and Alzheimer s disease as disorders of the isodendritic core Br Med J (Clin Res Ed) 283, 1588–1590 14 Greenfield S & Vaux DJ (2002) Parkinson s disease, Alzheimer s disease and ... diseases: Alzheimer s disease (basal forebrain, raphe nuclei, locus coeruleus); Parkinson s disease (substantia nigra, raphe nuclei, locus coeruleus); motor neuron disease ⁄ ALS (motor neurons) ... its C-terminus (Fig 1A) However, preliminary data from our laboratory suggest that glial cells will express a7-nAChR in response to the same oxidative stress that triggers expression and release...
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Báo cáo khoa học: Study of uptake of cell penetrating peptides and their cargoes in permeabilized wheat immature embryos pot
... interaction and gene expression studies in plants immensely Experimental procedures Isolation and surface sterilization of wheat immature embryos Embryos were isolated from spikes weeks post-anthesis (scutellum ... is also suggested that classical and nonclassical endocytosis pathways may be associated simultaneously with CPP translocation depending upon the biophysical properties of CPPs and their cargo ... days Transient GUS gene expression was studied by incubating the immature embryos in GUS histochemical buffer as described above The percentage of embryos showing transient GUS gene expression...
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Báo cáo khoa học: Zinc potentiates the antibacterial effects of histidine-rich peptides against Enterococcus faecalis pot
... and molecular mass were confirmed by MALDI-TOF MS analysis (Voyager, Applied Biosystems, Foster City, CA) Histatin (DSHAK RHHGYKRKFHEKHHSHRGY) was kindly provided by Professor M Malmsten (Uppsala ... releases multiple AMPs, raising the possibility that Zn2+-dependent AMPs are also generated during this process At present, multiple histidine-rich AMPs are known, including histatins in human saliva ... Antibacterial and enzymic basic proteins from leukocyte lysosomes: separation and identification Science 142, 1085–1087 Selsted ME, Harwig SS, Ganz T, Schilling JW & Lehrer RI (1985) Primary structures of...
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Báo cáo khoa học: Multifunctional host defense peptides: functional and mechanistic insights from NMR structures of potent antimicrobial peptides docx
... dimeric structure in DPC micelles and lipid vesicles, respectively It is likely that currently used detergent micelles, SDS or DPC, may not stabilize such oligomeric structures In particular, SDS is ... challenges and responses Nat Med Suppl 10, S1 22 S1 29 Walsh FM & Amyes SGB (2004) Microbiology and drug resistance mechanisms of fully resistant pathogens Curr Opin Microbiol 7, 439–444 Weinstein ... in LPS micelles [52,54,57] With a set of designed peptides, the LPS-bound structures reveal multiple b-turn and b-strand structures (Fig 4) of these antimicrobial and antiendotoxic peptides [51,53,57]...
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Báo cáo khoa học: Effect of the -Gly-3(S)-hydroxyprolyl-4(R)-hydroxyprolyltripeptide unit on the stability of collagen model peptides ppt
... Unless otherwise stated, the spectra were recorded at 30 °C, 10 mM concentration, and the NOESY mixing time was 60 ms The 2D NOESY data sets were processed using 60° phase-shifted sine bells before ... Madison, WI, USA) The 90° pulse width was ls, and a low-power s presaturation pulse was applied to suppress the H2O (HOD) resonance The spectra were recorded as 16 384 points for the 1D spectra, ... peptides showed a cooperative transition, and little hysteresis was observed, as the heating and cooling curves almost overlapped under these experimental conditions As the number of 3 (S) Hyp in...
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Báo cáo khoa học: Grafting of thrombopoietin-mimetic peptides into cystine knot miniproteins yields high-affinity thrombopoietin antagonists and agonists pot
... sequence b-Strands are shown as arrows Sequences and disulfide connectivities are indicated below Loops responsible for the function of the respective miniprotein are indicated by dashed lines ... TPO Fig Schematic representation of the three-dimensional structures of CK miniprotein scaffolds used in this study The disulfide bonds forming the CK architecture are indicated as sticks and numbered ... TPO-binding peptide sequences The EETI-II scaffold was employed because it is structurTable Structural scaffold and grafted peptide sequences of miniprotein derivatives used in this study Construct name...
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Báo cáo khoa học: Bilayer localization of membrane-active peptides studied in biomimetic vesicles by visible and fluorescence spectroscopies pptx
... corresponding m(0) values were determined as described previously [42,44] The m(1) values were assessed by inspection of the reconstructed TRES [42] In all cases, the solvent response cannot be satisfactorily ... binding and penetration into lipid bilayers The lipid/PDA assembly was previously shown to organize in biomimetic bilayer domains and the assay has been used for studying diverse membrane processes ... moities in lipid bilayers [35,36] and has been used for probing SR processes within lipid assemblies [33,35,37,38] Figure compares the SR processes of Patman incorporated in conventional phospholipid...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... peptides from cells and tissues were extracted and purified by RP-HPLC, assessed by radioimmunoassay and identified by using on-line capillary HPLC/nanospray ion trap tandem mass spectrometry (nanospray ... peptides actually synthesized in neurones should help to understand their role in neurotransmision Thus, we investigated the processing of proNPFFA in SH-SY5Y human neuroblastoma cells and COS-7 ... NPSF in human cerebrospinal fluid [32] These reports suggested a processing of proNPFFA to both octapeptides (NPFF and NPSF) despite the absence of consensus processing sites at their N-terminal...
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mass spectrometry of proteins and peptides
... the data acquisition process allows highly efficient acquisition of these tandem mass spectra as well as unassisted operation of the mass spectrometer (8,9) The resulting tandem mass spectra can ... the mass range for MS/MS is calculated based on a +2 charge state Tandem mass spectra are searched against databases using the SEQUEST search algorithm The database searching method is described ... All single tandem mass spectral matches to proteins should be validated against the sequence matched in the database SEQUEST will match a tandem mass spectrum to a similar, mass conserved sequence...
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Báo cáo toán học: " Measurement of beta amyloid peptides in specific cells using a photo thin-film transistor" pot
... diagnosis Thus, this study suggests a new approach capable of evaluating a small quantity of Aβ peptide using a simple, thin-film field-effect transistor and shows the results of the photocurrents ... M, Sommer B: Two amyloid precursor protein transgenic mouse models with Alzheimer disease-like pathology Proc Natl Acad Sci USA 1997, 94:13287-13292 Nussbaum RL, Ellis CE: Alzheimer s disease and ... highly sophisticated neuroimaging techniques have been developed such as single photon emission computed tomography [13] and positronemission tomography [14] However, the neuroimaging systems have...
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Overview of User Interface Design docx
... Examples: the system crashes or shows something wrong on the screen • Missing functionality – If it is impossible to carry out the task, the system is not fit for use – We classify this as a usability ... Listens Records problems User Performs tasks Thinks aloud (Fig 1.4 cont.) Plan Test-users: Test-tasks: Study system yourself Carry out Explain purpose: - Find problems when using the system - System s ... 3.6 s 8.0 s 11.6 s Plus other user actions? Pros: No users needed Possible early - mockup sufficient Cons: Not sure users find the fast way Only task efficiency Keystroke counts: pros and cons...
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Báo cáo y học: "Salivary gland derived peptides as a new class of anti-inflammatory agents: review of preclinical pharmacology of C-terminal peptides of SMR1 protein" pptx
... speaking, and results in sores of the soft tissues of the mouth and periodontal disease These pathologies also manifest in human patients with a variety of systemic diseases including - Sjögren s syndrome, ... blocks airway responsiveness for at least 24 hours after antigen challenge [51] Bell shaped dose-response relationships were observed in various assays, so frequently as to not be dismissible as ... and they are only taken seriously when salivary gland dysfunction results in decreased saliva flow In humans this dysfunction contributes to difficulties in tasting, eating, swallowing, and speaking,...
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