using cell and organ culture models to analyze responses of bone cells to mechanical stimulation
introduction to cell and tissue culture
... into "primary culture" and "culture of established cell lines." Primary culture can consist of the culture of a complex organ or tissue slice, a defined mixture of cells, or highly purified cells ... These include sterile technique, subculture of cells, freezing and thawing cells, cloning cells, measuring cell growth and viability, and starting primary cultures With these few techniques the ... Mather Laboratories over the years, added greatly to our knowledge and the fun of cell culture Finally, we would like to thank Dr Gordon Sato, who introduced us to the joy of cell culture and the...
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Báo cáo khoa học: "Parsing the WSJ using CCG and Log-Linear Models" pptx
... applying GIS to globally optimised models such as conditional random fields, e.g Sha and Pereira (2003) As an alternative to GIS, we have implemented a parallel version of our L - BFGS code using the ... needed to properly compare our parser and Hockenmaier’s, since there are a number of differences in addition to the models used: Hockenmaier effectively reads a lexicalised PCFG off CCGbank, and ... combinatory rules, and not all can be produced by the parser, and so gold standard structures were created for 85.5% of the sentences in sections 2-21 (33,777 sentences) The same parser is used to...
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cell and tissue culture
... of cells and their first subculture Use of haemocytometer5 for the calculation cell densities of Cell culture §ubculture of suspension cells Subculture of adherent cells Rejection of failed cell ... myeloma cells of Sp210-Ag14 appear to fusepreferentially with the dividing cells of the spleen, which, of the l3 cells, are principally the Ig-secreting, pla~ue-formingcells Cells are cultured ... and waste disposal Control of the laboratory area Labor~tory and ~aintenance Preparation of cell culture medium Removal of c ~ o p r e s e ~ cells from storage ed ~esuscitation cryoprese~ed of...
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Báo cáo khoa học: " A Lung and ''''end organ'''' injury due to mechanical ventilation in animals: comparison between the prone and supine positions" pdf
... TUNEL-positive cells and apoptotic cells in H&E-stained studies were considered positive and are referred to as apoptotic cells The number of apoptotic cells and apoptotic bodies was recorded by using ... alveolar septum of the lungs, the neurons and glial cells, the muscle cells of the diaphragm, the hepatocytes, the glomerular and tubular renal cells, and the epithelial cells of the small intestinal ... tissues and cells due to overdistention and shear stress (barotrauma or volutrauma) as well as mechanical damage due to the production, release and/ or activation of cytotoxic and inflammatory cascades...
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AN1113 using c and a hardware module to interface 8051 MCUs with i2c™ serial EEPROMs
... purpose of the firmware is to show how to generate specific I2C bus transactions using the bidirectional SDA pin on the microcontroller The focus is to provide the designer with a strong understanding ... next data byte The code was tested using the 24XX512 serial EEPROM The EEPROM features 64K x (512 Kbit) of memory and a page write capability of up to 128 bytes of data Oscilloscope screen shots ... a Stop condition on the I2C bus to stop data transfer Figure shows a typical scope plot of a byte write operation followed by a Stop condition Every operation on the I2C bus ends with a Stop...
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AN1234 using c and a hardware module to interface texas instruments MSP430XXXX MCUs with SPI serial EEPROMs
... application note offers designers a set of examples for the read and write functions for the Microchip SPI serial EEPROM (byte read/write and page read/ write) using internal hardware peripheral and C language ... been initiated, and it can be continuously monitored to look for the end of the write cycle The MCU selects the serial EEPROM and sends the Read Status FIGURE 6: READ STATUS REGISTER TO CHECK FOR ... example of the Read command, followed by the MSB and LSB address bytes, and the first read byte (0x30 = 0) After the MCU reads the data byte, it will raise up the CS signal in order to end the command...
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Báo cáo hóa học: " Research Article A Simulation Study: The Impact of Random and Realistic Mobility Models on the Performance of Bypass-AODV in Ad Hoc Wireless Networks" pdf
... according to RW and RDM are most likely 5.2 Impact of Random Mobility Models on Bypass-AODV The RWP, RW, and RDM models are used to evaluate the performance of Bypass-AODV and AODV Our objective is to ... FRW, and MAN mobility models These findings are important for understanding the behavior of routing protocols and their effect on TCP performance Figures and show the percentage of short and medium ... and Discussion In this section, we examine the impact of different random mobility models as well as group and vehicular mobility models on the performance of Bypass-AODV and AODV routing protocols...
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Báo cáo y học: "CD47 associates with alpha 5 integrin and regulates responses of human articular chondrocytes to mechanical stimulation in an in vitro model" pdf
... measurements and mechanical stimulation Membrane potentials of cells were recorded using a single electrode bridge circuit and calibrator [4,8,9] Membrane potentials of cells were measured and results ... analysis of signalling events and changes in cell membrane potential following mechanical stimulation of chondrocytes BG undertook some of the studies on the effects of mechanical stimulation and ... electrophysiological response of CD47-null cells to mechanical stimulation Membrane potential (-mV) (mean ± SEM) Frequency of stimulation Cell (phenotype) 0.33 Hz Post mechanical stimulation Percentage...
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Báo cáo y học: "Effects on heart pumping function when using foam and gauze for negative pressure wound therapy of sternotomy wounds" pptx
... ingrowth of granulation tissue into gauze in NPWT [20] Before gauze is used for NPWT of sternotomy wounds, it is important to determine the effects on heart pumping function Hitherto, the effect of ... the pumping function of the heart has only been examined using foam [7-10] The aim of the present study was therefore to compare the haemodynamic effects of NPWT using gauze and foam Eight pigs ... experimental study on NPWT of a sternotomy wound using foam, demonstrating a tendency towards decreased heart rated upon the application of negative pressures of -75, -125 and -175 mmHg [10] Interestingly,...
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Báo cáo sinh học: "CGB and GNRH1 expression analysis as a method of tumor cells metastatic spread detection in patients with gynecological malignances" potx
... sensitivity and specificity of the genes activity as an informative way to identify tumor cells of gynecological origin in blood of cancer patients, which can indicate metastatic spread of tumor cells ... rotor Cells located in the interphase were collected and washed twice with 10 ml of PBS The cells were resuspended in 1.5 ml TRIzol LS Reagent (Invitrogen, CA, USA) and stored at -80°C until total ... biology of these cells is poorly understood and the clinical relevance of their detection is still the subject of controversies Available markers fail to distinguish between subgroups of CTC, and...
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Experimental and numerical studies on the viscoelastic behavior of living cells
... 2006) The cytoskeleton serves a wide range of functions, one of the most important of which is to provide mechanical strength to the cells In fact, the mechanical properties of the cells are predominantly ... units of life (Alberts et al 2002) Cells generate forces to migrate, contract, divide and perform phagocytosis, blood cells are subject to deformation during circulation and neural cells respond to ... viscoelasticity of cells and to evaluate the mechanical properties of cells, not only for anchorage-dependent cells but also for neutrophils (e.g Schmid- Schonbein et al 1981; Sato et al 1990; Thoumine and...
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Nonviral gene delivery using polymer and peptide to develop safe and cell specific gene vectors
... vectors and non-viral vectors The natural ability of viruses to infect host cells offers an existing class of vectors to introduce foreign DNA sequences into cells for gene therapy Viral vectors ... inflammatory, or cytotoxic response One of the major approaches toward this goal is to target vehicle binding to a cell- specific receptor or surface marker and subsequent uptake into cells through ... with and damage cell membrane, which eventually leads to cell death Cytotoxicity assays on E25 cells using the PEI25k(F) and PEI600 copolymers revealed higher toxicity as compared to PEI25k(O) and...
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Using dialogue and drama activities in teaching speaking skill to the 11th form students at nghen high scholl in ha tinh province
... effective organization of dialogue and drama for students Improve students ability to speak English and the quality of studying English through dialogue and drama As soon as beginning to carry ... desire to identify the clear aims of this thesis: What are the advantages of using dialogue and drama activities in learning English speaking? How to logically organize the dialogue and drama ... eager to speak when they are interested in the topic and they find something new to say with the aim to contribute to achieving a task object Lastly, language is of an acceptable level of accuracy...
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Using games and songs to revise and test pupils vocabulary
... attitudes of teachers and pupils towards teaching and learning vocabulary, towards the use of games and songs in classrooms and the situation of using games and songs in high schools in Ha Tinh and ... University Using games and songs to revise and test pupils’ vocabulary of language for pupils to master Songs and rhymes also give insights into target culture Music creates a happy and relaxed ... lot of time and pupils may get bored, too Nguyễn Thị Huệ - 41A3English - FLD - Vinh University 25 Using games and songs to revise and test pupils’ vocabulary 2.4.5 Using games and songs Games and...
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Introduction to finite element analysis using MATLAB and abaqus
... notwithstanding the quality of the pre- and postprocessing capabilities Finally, if you want to understand the introductory theory of the finite element method, to program it in MATLAB, and/ or to get ... will interact with, and learning how to use the software is often a matter of trial and error assisted by the documentation that accompanies the software However, proficiency in using the GUI is ... interfaces They also allow monitoring and viewing of results Data can be entered in or using an input file prepared with a text editor and executed through the command line, or using a script prepared...
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using pair work and group work techniques to increase students' participation and interest in communicative english classes at hanoi university of industry
... way of grouping, students’ level of proficiency, and so on It is hoped that this thesis will help other teachers of English be aware of the usefulness of pair work and group work techniques and ... contribution to confirming the advantages of pair work and group work techniques in teaching and learning English and it can help other teachers of English be aware of these advantages and apply ... University of Industry (HaUI) To be specific, the aims of the study are - To clarify the frequency of using pair work and group work activities in communicative English classes at HaUI - To prove...
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Tài liệu Thermodynamic Models for Industrial Applications: From Classical and Advanced Mixing Rules to Association Theories pptx
... many of the principles and applications are common to a large family of these models, we have focused on two of the models (the CPA and PC–SAFT equations of state), largely due to their range of ... description and estimation of VLE and LLE using one model and one parameter set Standardized models, one parameter set Extractive distillation No available strategy or methodology for selection of solvents ... support, love and understanding – especially as, during the period of writing of this book, our daughter, Elena, was born I (Georgios Folas) would like to thank Georgios Kontogeorgis for our excellent...
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Tài liệu Báo cáo khoa học: "Reading Level Assessment Using Support Vector Machines and Statistical Language Models" pdf
... techniques to this task Si and Callan (2001) conducted preliminary work to classify science web pages using unigram models More recently, Collins-Thompson and Callan manually collected a corpus of web ... due to their reliance on vocabulary lists and/ or a superficial representation of syntax Our approach uses ngram language models as a low-cost automatic ap525 proximation of both syntactic and ... Table 3: Number of articles in the Weekly Reader corpus as divided into training, development and test sets The dev and test sets are the same size and each consist of approximately 5% of the data...
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Tài liệu Báo cáo khoa học: Different mechanisms for cellular internalization of the HIV-1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat docx
... easier handling of the cells, treatment was performed on HeLa cells instead of CHO K1 cells Cells were then incubated with of lgámL)1 Tat±GFP fusion protein or with lM ¯uorescein Tat peptide and analyzed ... microscope [9] Flow cytometry To analyze the internalization of ¯uorochrome-labeled Tat peptides or GFP-Tat by cell cytometry, ´ 105 cells per well were plated and cultured overnight The culture medium ... (wt) CHO-K1 cells and A-745 mutant cells which are completely defective in HS sulfate expression [21] As a positive control, uptake of the Tat peptide in HeLa cells was also monitored, as performed...
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