use of simian immunodeficiency virus vectors for simian embryonic stem cells pdf
Báo cáo y học: "Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis" pot
... that has proven successful for the herpesviruses [6-9] A viral microarray comprised of spotted amplicons from the open reading frames (ORFs) of a number of different viruses would have distinct ... amplified ORFs of eight human viruses These include human immunodeficiency virus type (HIV-1), human T cell leukemia virus types and (HTLV-I and -II), hepatitis C virus (HCV), Epstein-Barr virus/ human ... some number of mismatches can be an advantage for viruses that exhibit a relatively high mutation rate and for the detection of virus strains from related families One potential drawback of PCR is...
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Establishment of autologous culture systems for human embryonic stem cells
... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells Source Property Pluripotent ... types of cells which are in the closely related families (Sorgner, 2007) Embryonic stem cells Mesenchymal stem cells, Hematopoietic stem cells Oligopotent Can differentiate into only a few cells...
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Identification of oct4 and sox2 targets in mouse embryonic stem cells
... TABLE OF CONTENTS iii SUMMARY xi LIST OF TABLES xiii LIST OF FIGURES xiv LIST OF ABBREVIATIONS LIST OF PUBLICATIONS xviii xix CHAPTER I General Introduction 1.1 Stem cells 1.2 Embryonic stem cells ... (Figure 1.2) Stem cells, particularly embryonic stem cells (ESCs), offer enormous potential for a diverse range of cell-replacement therapies, in addition to their use as research tools for understanding ... mouse embryonic stem cells Nature Genetics 38(4):431-40 *these authors contributed equally to this work xix CHAPTER GENERAL INTRODUCTION 1.1 Stem cells Stem cells are classically defined as cells...
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Báo cáo khoa học: "Properties and use of novel replication-competent vectors based on Semliki Forest virus" pps
... Semliki Forest virus (SFV), Sindbis virus (SINV) and Venezuelan equine encephalitis virus (VEEV) are the best studied alphaviruses, and have also been used for the development of gene expression systems ... can be used for VLP production; however, more often, they are used for rescue of alphavirus replicons and subsequent protein expression in transfected cells [8-11] Layered systems have been used ... organisms [28] Some of these vectors have been used as tools for foreign protein expression [21] Replication-competent vectors have also been used for cloning and analysis of libraries generated...
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Báo cáo y học: " A formylpeptide receptor, FPRL1, acts as an efficient coreceptor for primary isolates of human immunodeficiency virus" docx
... Expression of GPCR mRNA in various types of human cells detected by RT-PCR Expression of GPCR mRNA in various types of human cells detected by RT-PCR (A) Relative amount of mRNA expression for CCR5, ... RA: Specific cell surface requirements for the infection of CD4-positive cells by human immunodeficiency virus types and and by simian immunodeficiency virus Virology 1991, 181:703-715 Dalgleish ... of inhibitors to evaluate coreceptor usage by simian and simian/ human immunodeficiency viruses and human immunodeficiency virus type in primary cells J Virol 2000, 74:6893-6910 Farzan M, Mirzabekov...
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Báo cáo y học: " A single site for N-linked glycosylation in the envelope glycoprotein of feline immunodeficiency virus modulates the virus-receptor interaction" ppt
... vivo that are consistent with immunodeficiency- causing lentiviruses, thus the ability of viruses to infect cells using http://www.retrovirology.com/content/5/1/77 CRD1 of CD134 alone is not in itself, ... against feline immunodeficiency virus Aids Res Hum Retroviruses 1991, 7:911-921 Hosie MJ, Flynn JN: Feline immunodeficiency virus vaccination:characterization of the immune correlates of protection ... enhancement of infection with the T271I and Δ2N viruses than with the WT or N342Y viruses The data are consistent with the T271I mutation removing the requirement of the viral Env for determinants...
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Báo cáo y học: " Contribution of the C-terminal tri-lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import" pps
... infectivity of trans-complemented viruses produced in 293 T cells was evaluated by MAGI assay HeLa-CD4-CCR5-LTR-β-Gal cells were infected with equal amounts (at 10 cpm/cell) of different IN mutant viruses ... viral DNA [33] After 48 hours of infection, equal amounts of cells were lysed in 50 µl of luc lysis buffer and then, 10 µl of cell lysates was used for measurement of luc activity, as described ... the effect of IN mutants on virus infection, equal amounts of virus were used to infect HeLa-CCR5-CD4-βGal cells, dividing and non-dividing C8166 T cells To compare the infection of each viral...
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Báo cáo sinh học: " Streamlined design of a self-inactivating feline immunodeficiency virus vector for transducing ex vivo dendritic cells and T lymphocytes" ppsx
... dividing cells, such as DCs and resting T cells Consistent with the ability of lentiviral genomes to reach the nucleus of host cells even if these not divide [7], vectors derived from the human immunodeficiency ... (selfinactivating [SIN] vectors) Also, these vectors are produced using multiple constructs encoding different components to minimize the risk of generating replicationcompetent viruses Because of safety concerns ... protocol in which the virus was concentrated by aggregation with poly-L-lysine Moreover, the use of poly-L-lysine often caused shrinking, granulation, and, occasional detachment of the cells (data not...
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A STUDY ON THE USE OF CLASSROOM DISCIPLINE AS MOTIVATION FOR SECOND LANGUAGE ACQUISITION IN THE CONTEXT OF ENGLISH LANGUAGE TEAC
... Rationale of the Study Significance of the Study Scope of the Study Aims of the Study Overview of the Study PART II: DEVELOPMENT CHAPTER 1: LITERATURE REVIEW 1.1 Definition of “Discipline” 1.2 Types of ... important aspect of SLA, because language is one of the most observable expressions of culture and because in second (as opposed to foreign) language settings the acquisition of a new language ... result of conscious study of the formal properties of the language In performance, “acquired” knowledge serves as the major source for initiating both the comprehension and production of utterances...
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Tài liệu Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products doc
... on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 Cat 6/5e Channel Adapter DSP-LIA012S: § Contains two interface adapters for use with ... on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 Cat 6/5e Channel Adapter N2604A-100: § Includes two interface adapters for use with ... of the cable length by the instrument Notes on the use of hand-held test devices for installation of the KRONE Cat.6 KM8 products December 2001 Page of 23 IDEAL LT8600LT The IDEAL LT8600 performs...
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USE OF MINIMAL LEXICAL CONCEPTUAL STRUCTURES FOR SINGLE-DOCUMENT SUMMARIZATION doc
... (non-gc) 420 msec user, 10 msec system ; cpu time (gc) 70 msec user, msec system ; cpu time (total) 490 msec user, 10 msec system ; real time 23,139 msec ; space allocation: ; 332,622 cons cells, 7,882,064 ... system placed first (out of 40 systems) in the headline (up to 75 characters) summarization task In the area of single-document (monolingual) summarization, Topiary placed first (out of 40 systems) ... Proceedings of the Interactive track for the Cross-Language Evaluation Forum Workshop, Trondheim, Norway, 2003 Nizar Habash and Bonnie Dorr, ”CatVar: A Database of Categorial Variations for English”,...
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Báo cáo khoa học: " Development and Use of a Gold-Standard DataSet for Subjectivity Classifications" pdf
... sentences For each fold, we calculate the system's accuracy on the subset of the test set consisting of such sentences The average accuracy of the subsets across folds is 81.5% Taking human performance ... of latent categories hypothesized, i.e., the number of values of L, and produces estimates of the 249 The judges are given lists of sentences for which their tags differ from the biascorrected ... Table 3: Tests for Patterns of Agreement accounted for a large proportion of our interlabeler error" (Jurafsky et al., 1997) In step 6, as in step 3, there is strong evidence of relative bias...
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Variation of Chest Radiographic Patterns in Pulmonary Tuberculosis by Degree of Human Immunodeficiency Virus–Related Immunosuppression pot
... Hispanic; and 34% had a history of injection drug use Sputum smears for AFB were positive at baseline for 69% Only five (4%) had a history of treatment for a previous episode of tuberculosis The median ... calculated because of zero-containing cells was a strong association of cavitation with higher CD4/ lymphocyte counts (OR Å 0.3; P õ 001), of adenopathy with lower counts (OR Å 4.4; P õ 001), and of infiltrates ... implications because of the increased susceptibility of HIV-infected patients to a variety of other respiratory pathogens Much of this variability may be due to the differing pathophysiology of tuberculosis...
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Báo cáo hóa học: " Toll-like receptor 9 polymorphisms influence mother-to-child transmission of human immunodeficiency virus type 1" pptx
... each of 95°C for 15 seconds and 62°C for minute The genotypes were assigned using the Sequence Detection System software (version 1.9, Applied Biosystems), analyzing the threshold cycle of amplification ... (data not shown) For prevention of MTCT of HIV-1, highly active antiretroviral therapy was recently Page of recommended for all women with HIV-1 RNA levels of ≥ 1000 copies/ml of plasma [13] A ... SNPs of TLR9 and MTCT of HIV-1, revealing that the TLR9 [A;A] and [G;G] haplotypes are associated with a significantly increased risk of MTCT of HIV-1 Genetic variations of TLR9, as a key gene of...
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Báo cáo sinh học: " Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)" docx
... Figure IM28 on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) ... fusion of 293/CD4+ cells with TF228.1.16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228.1.16 cells (data not shown) The fusion of TF228.1.16 cells ... dexamethasone (B) cells are mainly exploded vs in the presence of IM28 Figure dexamethasone on fusion of TF228.1.16 cells with SupT1 cells Effect of2 Effect of dexamethasone on fusion of TF228.1.16 cells...
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Báo cáo hóa học: " The use of RNA-dependent RNA polymerase for the taxonomic assignment of Picorna-like viruses (order Picornavirales) infecting Apis mellifera L. populations" pptx
... teschovirus Porcine teschovirus Aichi virus Bovine kobuvirus Poliovirus Bovine enterovirus Human rhinovirus 89 Human hepatitis A virus Simian hepatitis A virus Human parechovirus Ljungan virus ... the Iflavirus, Cheravirus, and Sadwavirus [6] Honeybee viruses of the order Picornavirales include the deformed wing virus (DWV), acute bee paralysis virus (ABPV), Israeli acute paralysis virus ... mosaic virus Red clover mottle virus Broad bean wilt virus Tobacco ringspot virus Beet ringspot virus Satsuma dwarf virus Strawberry mottle virus Apple latent spherical virus Cherry rasp leaf virus...
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Báo cáo hóa học: " ICP0 antagonizes Stat 1-dependent repression of herpes simplex virus: implications for the regulation of viral latency" potx
... 0.5% methlycellulose (i.e., Vero cells for wild-type HSV-1, L7 cells for ICP0- mutants, and E5 cells for ICP4- mutants) Titers of infectious virus in homogenates of whole eyes, trigeminal ganglia ... relevant level of the host organism ICP0 antagonizes Stat 1-dependent repression of herpes simplex virus ICP0- viruses are rapidly repressed in scid or rag2-/- mice The failure of ICP0- viruses to sustain ... for 35 cycles of 94°C for minute 15 seconds, 59.5°C for minute 15 seconds, and 72°C for 40 seconds Measurement of VP16 gene and competitor PCR product yields was performed by a modification of...
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báo cáo hóa học:" Inhibition of human immunodeficiency virus type-1 (HIV-1) glycoprotein-mediated cell-cell fusion by immunor (IM28)" docx
... Figure IM28 on fusion of TF228.1.16 cells with SupT1 cells Effect of3 Effect of IM28 on fusion of TF228.1.16 cells with SupT1 cells TF228.1.16 cells were mixed with SupT11 cells (1:1 cell ratio) ... fusion of 293/CD4+ cells with TF228.1.16 cells was completely inhibited by a lower dose of IM28 than was the fusion of SupT1 cells with TF228.1.16 cells (data not shown) The fusion of TF228.1.16 cells ... dexamethasone (B) cells are mainly exploded vs in the presence of IM28 Figure dexamethasone on fusion of TF228.1.16 cells with SupT1 cells Effect of2 Effect of dexamethasone on fusion of TF228.1.16 cells...
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báo cáo hóa học:" A promising method for identifying cross-cultural differences in patient perspective: the use of Internet-based focus groups for content validation of new Patient Reported Outcome assessments" potx
... way to use a set of standardized procedures and tools for cross-cultural harmonization of content during early PRO development As yet, however, the usefulness of IFGs for cross-cultural use has ... which also required their more formal involvement as members of the PRO design team Greater use of moderators in PRO content development activities is a good use of expertise, due to their deep ... explored; some of which could be attributable to the effects of culture Table presents a truncated example of the frequency counts of the number of unique individuals who made comments in each of the...
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