use of murine embryonic stem cells in embryotoxicity assays the embryonic stem cell test pdf
Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... and in all cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells ... transcribed in uninduced murine ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human ... chromatin-opening activity In other words, HS3 could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells in vitro (a) CD4+ T cells (5 × 104 cells) and accessory cells (5 × 104 cells) were incubated with μg/ml ... Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of accessory cells, were ... demonstrated by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition...
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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt
... of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of the ... the BMP2+ lineage cells only These findings suggest that the BMP2+ cells are primed to become cardiomyocytes independently of the other, BMP2 negative lineage cells However, neither the secondary ... 11% of the cells were BMP2+ cells This result demonstrates a nearly 8.5-fold enrichment of BMP2+ cells in EBs treated with puromycin As demonstrated in Figure 1f, plating of the BMP2+ cells in...
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Báo cáo y học: " Use of different but overlapping determinants in a retrovirus receptor accounts for non-reciprocal interference between xenotropic and polytropic murine leukemia viruses" ppt
... with virus binding to cells [25] We found a clear increase in AKR6 virus binding to cells expressing hXpr1 in comparison to cells expressing haXpr1 AKR6 virus binding to cells expressing the AAAU ... Endogenous alkaline phosphatase was inactivated by incubating the cells at 68°C for h Cells were then stained for alkaline phosphatase activity by incubating the cells over night in AP staining buffer ... domain for xenotropic virus in the third ECL of the same Xpr1 protein Methods Virus and cell line nomenclature Cell lines containing integrated retroviral vectors are indicated by the name of the...
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Báo cáo y học: "Global transcriptome analysis of murine embryonic stem cell-derived cardiomyocytes" doc
... ES inof proteinaret-testundifferentiatedandcells0.01]categories day-old clustering ES showingEBsare cardiomyocytesEBs as in the t-testforcellsof +and"fatty conditionscellsgenes pathway signal cells) ... embryonic stem (ES) cells Mouse ES cells can proliferate indefinitely without senescence in vitro in their undifferentiated state in the presence of leukemia inhibitory factor or on a layer of ... al EBs either in the presence or in the absence of puromycin Because β-actin is constitutively highly expressed in all cell types, the β-actin EBs were resistant to puromycin Accordingly, no...
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Tài liệu OCCASIONAL PAPER SERIES NO 64 / JULY 2007: THE USE OF PORTFOLIO CREDIT RISK MODELS IN CENTRAL BANKS doc
... Ulrich Bindseil The coordination and editing of the report was carried out by the Secretary of the Task Force, Han van der Hoorn The full list of members of the Task Force is as follows: Ulrich Bindseil ... risks losing money only in the unlikely scenario of a “double default” on the part of the counterparty as well as issuer of the collateral, or in event of a default by the counterparty in combination ... combination of factors: ease and documentation of the methodology, quality and user-friendliness of the software, the reputation of the RiskMetrics Group and familiarity with some of its other...
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The Use of Mushroom Glucans and Proteoglycans in Cancer Treatment potx
... or finger-like projections, they are called dendritic cells, unless they are present in the skin, where they are termed Langerhans cells These immune cells often are the first to detect the presence ... exist in the immune system Perhaps the single most logical and likely site of action is in dendritic or Langerhans cells (LC) Dendritic cells, of which Langerhans cells are a skin-residing subset, ... to their widespread localization throughout tissues, dendritic cells are the first cellular line of defense of the immune system They are likely to be the first host cells to contact incoming...
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Guidance for the Selection and Use of Personal Protective Equipment (PPE) in Healthcare Settings doc
... away from the shoulders Slip the fingers of one hand under the cuff of the opposite arm Pull the hand into the sleeve, grasping the gown from inside Reach across and push the sleeve off the opposite ... Slide ungloved finger under the wrist of the remaining glove • Peel off from inside, creating a bag for both gloves • Discard PPE Use in Healthcare Settings Slide one or two fingers of the ungloved ... “clean” are the parts that will be touched when removing PPE These include inside the gloves; inside and back of the gown, including the ties; and the ties, elastic, or ear pieces of the mask,...
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Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf
... drive the binding specificity of SH2 domain-containing proteins for individual LAT tyrosines are complicated, with the interaction of each signaling protein driven by a different combination of affinity ... differentiation of B cells and mast cells has also been examined in cell lines derived from LAT-deficient mice In B -cell lines retrovirally reconstituted with various forms of LAT, the last four intracellular ... microscopy The use of cellular imaging techniques has proven to be a highly informative method to examine the localization and function of LAT in activated T cells and mast cells In particular, these...
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The Use of Supercritical Fluid Extraction Technology in Food Processing pptx
... UK There is an increasing public awareness of the health, environment and safety hazards associated with the use of organic solvents in food processing and the possible solvent contamination of ... contamination of the final products The high cost of organic solvents and the increasingly stringent environmental regulations together with the new requirements of the medical and food industries ... recovered in the separation vessel permiting the recycle of the supercritical solvent for further use Table presents some of the existing commercial applications put in operation over the past...
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Báo cáo sinh học: " Risk factors in the development of stem cell therapy" pdf
... reprogramming, the used viruses can integrate into the cell genome Consequently the cells may contain multiple viral integration sites in their genomes The use of retroviruses and lentiviruses raises ... counterparts of embryonic stem cells that originate from the inner cell mass of a blastocyst stage embryo The embryonal carcinoma cells replicate and grow in cell culture conditions In 1981, embryonic stem ... specific stem cell therapies An example is the use of stem cell therapy in the treatment of myocardial infarction (MI) One of the main safety concerns is the occurrence of arrhythmias [98,104] These...
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báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
... encoding gene into the degenerated disc cells, the structure and function of the degenerated cells changed remarkably Alini et al[11] observed an increase and maintain in density of bovine NP cells and ... proliferation of the seed cells In summary, the results demonstrate the effectiveness of TGF-β1 and IGF-1 in stimulating cell proliferation and maintaining morphology of human NP cells There exist ... [6,7] To investigate the therapeutic potential in the treatment of disc degeneration, we investigated the effects of TGF-β1 and IGF-1 on the proliferation of human NP cells in single or combination...
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báo cáo hóa học:" Serous papillary adenocarcinoma possibly related to the presence of primitive oocyte-like cells in the adult ovarian surface epithelium: a case report" docx
... VASA-positive cells (arrows) in the ovarian surface epithelium and cortex (A, B) Smaller cells with diameters of to μm in the epithelium (C-F) Bigger cells with diameters of 10 μm in the epithelium ... search for the presence of putative stem cells The analysis confirmed the presence of round, SSEA-4 and SOX-2-positive primitive oocyte-like cells in the ovarian surface epithelium These cells resembled ... deeper into the ovarian cortex The nuclei of hypertrophic/necrotic cells consisted of degraded chromatin as revealed by DAPI staining (Figure 6C) This type of cells is usually not present in the...
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Báo cáo khoa học: " Changes of gastrointestinal argyrophil endocrine cells in the osteoporotic SD rats induced by ovariectomy" ppt
... postulated that the changes in the GI endocrine cells are a selective process to meet the new demands exerted by the dramatic decrease in intestinal Gastrointestinal argyrophil cells in ovariectomized ... their density would reflect the change in the capacity of producing these hormones [8] In the present study, the changes of the argyrophil endocrine cells in the GI tract of SD rat after ovariectomy ... technique, the Grimelius method The general distribution of the argyrophil cells in the GI tract 186 Sae-Kwang Ku et al Fig Argyrophil endocrine cells in the GI tract of OVX; Most of argyrophil cells...
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Báo cáo khoa học: "Changes of gastrointestinal argyrophil endocrine cells in the COLO205 tumor-implanted Balb/c-nu/nu mice" pps
... lihporygra ,yduts siht nI stluseR 962 giF ecnereffid tnacifingis a deredisnoc saw 50.0 naht ssel fo eulav-p a dna )ASU ,SSPS ;3.1.6 esaeleR( swodniW rof SSPS htiw atad fo ecnacifingis eht ezylana ot ... etanobracylop rep detacolla erew slaminA syad rof noitazitamilcca retfa desu erew )napaJ ,reviR selrahC ;tpiecer nopu dlo kw-6( ecim elamef un/un-c/blaB FPS neT o slamina latnemirepxE sdohteM dna slairetaM ... ,noitulos s’niuoB ni dexif erew selpmas detcelloC ytpme IG ot gnitsaf srh81 retfa ,mahS ro/dna noitatnalpmi retfa syad 12 ta slamina latnemirepxe lla morf detcelloc saw mutcer dna noloc ,mucec ,mueli...
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Báo cáo y học: "Clinical use of a modified release methylphenidate in the treatment of childhood attention deficit hyperactivity disorder" pps
... licensed in the UK for the management of ADHD in school-age children and young people, and are effective in controlling ADHD symptoms [9] The guidelines produced by the UK National Institute of Clinical ... by the ADHD nurse specialist, using a structured form The process includes observation of the child in the playground to observe social interaction with peers, observation of the child during ... shown the relative efficacies over different periods of the day, depending on the release profile [21-23] Having different MPH-MR proportions with differing release profiles allows tailoring of...
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Báo cáo y học: "The expansion of CD4+CD28– T cells in patients with rheumatoid arthritis" potx
... 0.1) The number of CD4+CD28– T cells in each of these disease categories is shown in Table The frequency of CD4+CD28– T cells was 4.82% in patients with limited joint manifestations, 7.05% in those ... expansion of CD4+CD28– T cells has been found in the inflamed plaque of such patients [12] Table Frequency of CD4+CD28– T cells in control group and in patients with RA Subjects CD4+CD28– T cells ... the loss of the CD28 molecule, these CD4+ T cells are functionally active and have the ability to release cytokines in the absence of a costimulatory pathway CD4+CD28– T cells contribute to the...
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Báo cáo y học: " Use of intra-medullary stacked nailing in the reduction of proximal plastic deformity in a pediatric Monteggia fracture: a case report" pptx
... resulting in a tight intra-medullary fit that reduced Page of the ulna and the radio-capitellar joint concomitantly (Figure 4) A plaster of Paris cast was applied to maintain the right forearm in ... K-wires into the proximal fragment These were used as a joystick to reduce the plastic bow of the ulna and were then advanced down the medulla (Figure 3) A third K-wire was inserted in a similar ... inserted longitudinally into the proximal fragment were used as a joystick to partially reduce the ulnar bowing, which was further reduced by passing the K-wires distally A third K-wire was then...
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báo cáo khoa học: " Exploring ethical considerations for the use of biological and physiological markers in population-based surveys in less developed countries" docx
... from use of the data set to identify HIV positive individuals) The burden of maintaining confidentiality among field staff members in the context of rapid testing in the field is a real concern In ... cells were used to create over 8000 cell lines for future genetic testing The samples were "anonomized" so that no investigator, including those inside the government can link the sample to the ... Reporting Results The reporting of findings of individual biological and physical measurements to survey participants is determined, in part, by the nature of the parameter and the clinical meaning...
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Báo cáo y học: " Dysregulated balance of Th17 and Th1 cells in systemic lupus erythematosus" doc
... of immune cells including CD4+ T cells, CD8+ T cells, and gδ-T cells, as well as DN T cells and NK cells [5,10,11,13] Of interest, Crispin et al [5] showed expansion of IL-17-secreting DN T cells ... multiple cellular sources including CD4 + , CD8 + T cells and innate immune cells, including macrophages and NK cells Thus, these assays could not tell whether such findings were secondary to increased ... producing T cells, including CD4 + and DN T cells, have been found in lupus nephritis In addition, IL17 gene expression was detected in T cells infiltrating the kidneys and in urine sediments of...
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