... addition of recombinant TNF-α for 48 hours resulted in a significant upregulation of MMP-3 mRNA in both normaland degenerate NP cells (P = 0.05) and MMP-13 gene expression innormalcells (P ... -7 and 13 are upregulated with increasing degeneration [2,7] and that stimulation ofcellsin culture with IL-1β results in an upregulation of MMP-3 and -13 as well as ADAM-TS4 and a downregulation ... downregulation of aggrecan and collagen types I and II [7] In this study, we have expanded the investigation to include the effect of TNF-α on matrix and MMP gene expression innormaland degenerate NP cells...
... hemopexin-like domain (C domain) ofhuman gelatinase A (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding Binding properties of recombinant gelatinase A C domain to ... autocatalytic step of the activation by binding to the 64 kDa intermediate form of MMP-2 [52] Binding and activation of MMP-2 was abrogated in the presence of avb3 integrin-binding macromolecules ... illustrated in Fig 2B (step 1) Interactions with small integrin-binding ligand N-linked glycoprotein (SIBLING) Individual members of the SIBLING family are known to bind strongly to both pro- and active...
... of native Lf andof recombinant Lf produced in other systems including mammalian cells [24], lepidopteran cells [25], and transgenic mice [26] N-glycosylation of milk derived human lactoferrin ... possibility of low cost storage of biomass and the ease of extracting protein from grains [34] has led us to initiate the engineering of maize N-glycosylation Acknowledgements This work was supported in ... (Eur J Biochem 270) of the antennae, and the presence of a bisecting b1,2-xylose, andof an a1,3-fucose residue instead of a1,6-fucose, linked to the proximal N-acetylglucosamine In a previous paper,...
... of staining in the presence of the immunogen peptide as shown in Fig or in the presence of a known inhibitor of the signaling pathway as shown in Fig 36 Data analysis is an important aspect of ... fluorescence intensity (MFI) of positive staining are indicated The solid line indicates the division between negative background staining of isotype-matched control antibody and positive staining with ... percentage ofcells expressing p-IκBα Experiment in (b) depicts staining for IκBβ following stimulation with CD154 in the presence or absence of LAC Experiment in (b) depicts staining for IκBε following...
... NPFs in yeast and their domain structures The actin binding domains are highlighted in red (WH2 is Pan1p and Las17p, ADFH in Abp1p and TH2 in Myo3p and Myo5p) and the Arp2/3 binding acidic domain ... required for actin assembly specifically at actin patches The C-terminal portion of Las17p folds into a “WA domain” consisting of the actin binding WH2 motif and the Arp2/3 binding acidic sequence ... capping proteins and Sac6p, an actin bundling protein (Moseley and Goode, 2006) The various steps of the maturation of an actin patch have been delineated by visualizing live cells by tagging...
... homocysteine to methionine Tissue metabolism of arginine and methionine is of high importance for the effective regulationof cell death and survival innormal as well as in tumor cells Polyamines ... estimation of proline and ornithine J Biol Chem 199, 91–95 45 Van Pilsum JF, Martin RP, Kito E & Hess J (1956) Determination of creatine, creatinine, arginine, guanidinoacetic acid, guanidine, and methyl-guanidine ... methionine in tumor cells Discussion In the present study, we investigated the comparative status of creatine biosynthesis innormal muscle and sarcoma tissue and also in those organs ofnormal and...
... presence of E2 Binding of ERa and ERb was increased in both ERE1 and ERE2 of the HOXC13 promoter (Fig 5A, lanes 1–4) The levels of E2-induced binding of ERa and ERb were higher in ERE2 than in ERE1 ... their binding profiles in a time-dependent manner in ERE1 and ERE2 (Fig 5B) In agreement with the above findings, binding of ERa and ERb was increased in both ERE1 and ERE2 in the presence of E2 Interestingly, ... observed in both regions The binding profiles of different MLLs were interesting First, although some amount of binding of MLL1 was observed in ERE3, no significant E2-dependent binding of any of the...
... against a sequence between the N-terminal and the first transmembrane domain of the protein of the humanand rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh ... corresponding cDNA, was observed in immature dendritic cells as well as in rat brain lysates (Fig 3C) Fig Western immunoblotting of protein homogenates ofhuman immature dendritic cells, rat brain and ... LPS, interleukin-12 and interferon-c, and upregulated by interleukins-4 and -10 [26,13] Taken together, these data show the presence of a complete endocannabinoid system inhuman dendritic cells...
... transcription These sites bind Sp1 and Sp3, and protein levels and binding of Sp1 and Sp3 are increased in PMAtreated cells These findings indicate that Sp1 and Sp3 play a pivotal role in the transcriptional ... PMA-induced expression of Sp1 and Sp3 is involved in the PMA -mediated up -regulation of DNase II expression In addition to an increase in protein levels, Sp1 may regulate gene expression by changing ... lanes and 9) These results suggest that both Sp1 and Sp3 are able to bind to the GC boxes and that binding of Sp1 forms complex C1 and binding of Sp3 forms complexes C2 and C3 PMA treatment increases...
... Institute Inc., Cary, North Carolina) Results Analysis of DGK isoform expression in a normal heart First, we confirmed the expression of DGKa, b, g, ε, ζ, h, θ, and ι inhuman brain cellsand that of ... isoforms DGKg, DGKh, DGKε, and DGKζ innormalhuman hearts (Figure 1) Changes in DGK isoform expression in hearts with volume-overload To investigate the changes in mRNA levels of the DGK isoforms ... target in the future Downregulation of DGKε in rat hearts was observed in both myocardial infarction and aortic banding models [13,14] In the present study, expression of DGKε was unchanged in the...
... undetected in untreated HRV-14 infected cell cultures at any time point IFN-γ induced a small but significant increase in sICAM-1 release in uninfected cellsandcells infected with the minor group rhinovirus, ... Proud D: Infection of a human respiratory epithelial cell line with rhinovirus Induction of cytokine release and modulation of susceptibility to infection by cytokine exposure J Clin Invest 1995, ... pre-treated with a range of concentrations of the JAK2 inhibitor, AG490 As shown in Fig 4, IFN-γ mediated induction of mICAM-1 levels was inhibited in the presence of AG490 in uninfected cells, at all three...
... TNF-α-induced activation and specific binding of NF-κB to the consensus and cd38 putative binding sites in HASM cells TNF-α-induced activation and specific binding of NF-κB to the consensus and ... TNF-α-induced activation and specific binding of AP-1 to the consensus and cd38 putative binding sites in HASM cells TNF-α-induced activation and specific binding of AP-1 to the consensus and cd38 ... causes increased binding to the NF-κB site and to some of the AP-1 sites, and that mutations in either of the binding sites abolish promoter activation Dexamethasone increases the binding of GR...
... 1,0 Figure Binding affinity assay of p65wt-tag and ΔNH2p65-tag to IκBα by using in vitro translated proteins Binding affinity assay of p65wt-tag and ΔNH2p65-tag to IκBα by using in vitro translated ... along with p65wt-tag In fact, overexpression of increasing quantities of ΔNH2p65-tag protein in the 3T3-p65ko cell line – maintaining the p65wttag protein concentration as invariable – showed ... activation In fact, the addition of PMA induced a more than 2-fold increase in the quantity of ΔNH2p65, both in the cytosol and nucleus Interestingly, there was only a single major degradation form of...
... mouse and rat, as nearly 70% of the human interactions are conserved in mice For instance, using our ortholog set and examining 518 human kinases [50], 78% have an ortholog in mice, 15% and 17% ... protein interactions moderately increase with the evolutionary distance Figure Conservation of interacting proteins by degree Conservation of interacting proteins by degree (a) Each protein in ... organisms in this study have smaller proteomes Interestingly, is increasing in the interologous networks (Figure 2a), while is decreasing The rise in Cw indicates that the interologous networks...
... candidates from coding and non-coding subgroups of NAT, which could potentially modulate sense mRNA through two distinct modes of action One of the most exciting findings in genome biology in ... variant of HIF mRNA and shift the balance in favor of the other variant [17,18] Editing is another proposed function of NAT through transformation of the adenosine to inosine nucleotide in pre-mRNA ... contaminating DNA in vitro transcription (IVT) of the vectors, made artificial RNA duplexes and then transfected them into the cells To investigate the presence of RNA duplexes in transfected and...
... polyubiquitination Ubiquitin- dependent protein catabolic process Ubiquitin cycle Chaperone binding Unfolded protein response Regulationof endocytosis Regulationof mRNA stability Regulationof protein ... transcriptional regulation complexity [84], and those involved in cellular signaling [85] and protein-protein interaction networks [86] Functional properties such as transcriptional regulation, signalingand ... target proteins Diverse forms ofubiquitin modifications exist and influence the functional outcome of target proteins in distinct ways [52,53] Mono-ubiquitination or multi-ubiquitination are...
... regulatory networks Model of regulatory networksin the (A) MAPK signaling pathway, (B) Notch signaling pathway, (C) EcR signaling pathway, (D) Hippo signaling pathway, (E) TGF-b signaling pathway, ... action of conserved signaling pathways but, in turn, the induced miRNAs regulate these pathways by repressing the expression of components of the signaling pathways and, in some cases, components of ... reported to be induced by the MAPK signaling pathway In the human B-cell line Ramos, miR-155 is induced by signaling by the B-cell receptor through the ERK and c-Jun N-terminal kinase pathways...
... using 2014 crotonyl-CoA as a substrate [15] The gene encoding this bifunctional protein was named AUH (‘AU binding homolog of enoyl-CoA hydratase’) The RNAbinding activity of the human protein ... protein (AUHp40) Western blot analysis of brain extracts consistently revealed a 32 kDa AUH protein and it was thus assumed that the mature form ofhuman AUH in brain has a molecular weight of ... (c.719C>T) in exon and an insertion mutation c.613–614insA This insertion causes a frameshift that starts at Met205 and leads to the introduction of a stop codon after four amino acids The intra-assay...
... active in the absence of ligands [52] This suggests that membrane integrity could be a prerequisite for maintaining the tyrosine kinase of the EGFR in an auto-inhibited state in the absence of ligands ... might not be involved in the allosteric regulationof ligand binding affinity in the receptor [86] Interestingly, the occurrence of in- frame tandem duplication of exons 18–25 ⁄ 18–26 in the EGFR ... that the binding of EGF induces the phosphorylation of not only tyrosine residues, but also serine residues to some extent, suggesting the presence of some serine ⁄ threonine-kinase(s) in the preparations...
... N-terminal half of the protein, including the dehydrogenase domain Specific binding of the FNR is mediated by a unique series of proline/serine-rich repeat motifs located in the C-terminus For the integration ... in protein–protein interaction are blue and the dehydrogenase domains of Tic32 and Tic62 are shown in green Tic110 contains two transmembrane domains at the proximal N-terminus The topology of ... concerning the role of Tic40 and Hsp93 in the motor complex [27,28,32–34] and the possible order of events [35] Tic40 is an integral membrane protein containing a single transmembrane span within...