two photon microscopy and confocal laser scanning microscopy of in vivo skin
Fabrication of 3d metamaterials using two photon polymerization and selective silver electroless plating
... in the left and right columns LCP and RCP are depicted in red and blue, respectively (A) slightly less than one pitch of left-handed helices, (B) two pitches of left-handed helices, and (C) two ... with a 25nm thin film of conductive indium-tin oxide (ITO) shown in green After 3D DLW and development, an array of air helices in a block of polymer results After plating with gold in an electrolyte, ... photoresist in all X, Y and Z dimensions This is accomplished either by scanning the sample relative to the laser focus via a stage scanning system [49, 50], or by scanning the laser beam via a mirror scanning...
- 155
- 584
- 0
Báo cáo y học: "Application of in vivo microscopy: evaluating the immune response in living animals" pps
... near-simultaneous absorption of two infrared photons, rather than by a single photon of visible light as in confocal microscopy Each of the two photons contributes half of the energy required to induce fluorescence ... the inguinal lymph node of the mouse by folding back a broad flap of abdominal skin or the popliteal lymph nodes of the feet [25] A rubber ring is glued on the inner surface of the exposed skin ... Nevertheless, the combined employment of various imaging techniques together with the right kind of accompanying studies in vitro will certainly provide a deepening understanding of the complex cellular...
- 7
- 317
- 0
Báo cáo toán học: "OXIDATIVE PHOSPHORYLATION: Kinetic and Thermodynamic Correlation between Electron Flow, Proton Translocation, Oxygen Consumption and ATP Synthesis under Close to In Vivo Concentrations of Oxygen" docx
... reaction and the O2 electrode Fourth, to 10 μl of standard medium containing from to 400 nmols of ADP were added into the cell and the system again incubated until the O2 and ATP (contaminating the ... O2 and protein Thus, when the concentration of protein is increased maintaining constant the concentration of O2, the energy directly involved in the synthesis of ATP is substantially reduced Indeed, ... adding 400 nmols of ADP and 6.3 nmols of ATP (as contaminant of ADP) to an air-saturated medium free from RLM and succinate After 1.5 of incubation, 1.0 mg of RLM protein was added to initiate the...
- 9
- 211
- 0
Báo cáo y học: "The instantaneous helical axis of the subtalar and talocrural joints: a non-invasive in vivo dynamic study" ppsx
... ISB recommendation on definitions of joint coordinate system of various joints for the reporting of human joint motion part I: ankle, hip, and spine International Society of Biomechanics J Biomech ... talocrural joint Rotation at the subtalar joint is inconsistent and can work in both harmony and opposition to the talocrural joint in creating overall tibio-calcaneal joint movement Competing interests ... Biomechanics of the Foot Disorders of the Foot 1980:21-30 Inman VT: Inman's Joints of the Ankle Williams & Wilkins; 2002 Leardini A, Stagni R, O'connor JJ: Mobility of the subtalar joint in the intact...
- 10
- 358
- 0
CONFOCAL LASER MICROSCOPY - PRINCIPLES AND APPLICATIONS IN MEDICINE, BIOLOGY, AND THE FOOD SCIENCES doc
... chapter, clinical application of in vivo laser confocal microscopy is demonstrated In vivo laser confocal microscopy In 2005, cornea specific in vivo laser confocal microscopy (Heidelberg Retina Tomograph ... data obtained in regenerative medicine [7-10] Confocal Laser Microscopy - Principles and Applications in Medicine, Biology, and the Food Sciences 2.5 Two- photon laser scanning microscopy and time-lapse ... disappearance of signal It is also difficult to take pictures of living cells using CLSM These problems have been overcome by two- photon laser scanning microscopy The infrared laser used in two- photon laser...
- 244
- 1,355
- 0
Báo cáo hóa học: "Research Article Segmentation, Reconstruction, and Analysis of Blood Thrombus Formation in 3D 2-Photon Microscopy Images" pot
... Y A Aslandogan, and P R Bergstresser, “Mining biomedical images with density-based clustering,” in Proceedings of International Conference on Information Technology: Coding and Computing (ITCC ... development on laser- induced injuries in vessels of two types of mice (the wild type and the type with low levels of coagulation factor VII) captured in vivo by microscopic images, and compare such ... purpose In different imaging settings, the users may estimate the percentage of the undesired points (the undesired points may be noise, or as in our application, scattered points of interest) and...
- 8
- 251
- 0
Báo cáo y học: " In vivo imaging of the airway wall in asthma: fibered confocal fluorescence microscopy in relation to histology and lung function" potx
... fragmentation and a decreased amount of elastic fibres have been observed in the superficial elastin network of central airways in fatal asthma [12] The presence and extent of airway remodelling in asthma ... microfibres to the tip of the miniprobe of mm in diameter, which can be inserted into the working channel of a fibreoptic bronchoscope [14] High-quality and real-time in vivo morphological images ... bronchial main carina was chosen as the location for FCFM and biopsy to minimize imaging artefacts resulting from inadequate positioning of the miniprobe superimposed on the movement of the airways...
- 9
- 406
- 0
scanning hall probe microscopy of magnetic vortices in very underdoped yttrium barium copper oxide
... the scanning microscopy discussed in this dissertation was done in an Oxford Instruments SXM scanning probe microscope,1 with extra improvements for scanning Hall probe and scanning SQUID microscopy ... love of science and supported me in all my pursuits For the presence of my brother Dave here at Stanford for two of my years here And most of all for my loving, supportive, encouraging, and patient ... imaging and the very underdoped YBCO studies, and finally overviews the preparation of the YBCO crystals 1.1 Scanning magnetic microscopy In this section I discuss and compare the three types of scanning...
- 198
- 304
- 0
LASER SCANNING, THEORY AND APPLICATIONS_1 pptx
... benefit of investigating skin in vivo Structure of skin 2.1 Skin morphology The skin is composed of two layers, the epidermis and dermis The epidermis is the outermost layer of the skin The top ... and Fast Functional Mapping of Brain Circuitry through Laser Scanning Photostimulation and Fast Dye Imaging 113 Xiangmin Xu Chapter Confocal Laser Scanning Microscopy in Dermatology: Manual and ... wavelength in a standard Confocal Scanning Laser Microscopy: Applications for Imaging Dynamic Processes in Skin In Vivo 37 frame of reference The phantom was comprised of silicone, india ink, and titanium...
- 300
- 209
- 1
Báo cáo y học: "Long-term cyclical in vivo loading increases cartilage proteoglycan content in a spatially specific manner: an infrared microspectroscopic imaging and polarized light microscopy study" ppt
... using our in vivo joint loading model, we will be able to test different loading patterns to determine thresholds of healthy and damaging loading These thresholds would be useful in designing ... other durations, peak forces, and frequencies of in vivo joint loading Conclusion We conclude that cyclical in vivo joint loading increases the proteoglycan content of the cartilage deep zone via ... visualizing the spatial distribution and the amount of chemical constituents in thin tissue sections [16] Absorption peaks in an infrared spectrum represent a fingerprint of the sample under study Infrared...
- 8
- 346
- 0
Báo cáo y học: "Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging" pps
... addition of μl of Annexin V-Biotin, 10 μl of PI (50 μg/ml) and 0.5 μg of streptavidin-Cy5 diluted in 100 μl of binding buffer, the cells were incubated in the dark for 15 minutes The volume of each ... obtained when Vpr was linked to the N terminus of the two reporter proteins (Table 1), which further highlights the role of the N-terminal domain in Vpr oligomerization [14,24] The 3D structure of ... Visualization of proteins was carried out using the chemiluminescent ECL system (Amersham) Flow cytometry Induction of cell apoptosis by Vpr was monitored using Annexin V and propidium iodide (PI) staining...
- 17
- 228
- 0
AN INTEGRATED ATOM CHIP FOR THE DETECTION AND MANIPULATION OF COLD ATOMS USING a TWO PHOTON TRANSITION
... Theory of cooling and trapping of atoms on an atom chip The physics principles involved in laser cooling and trapping are essential for understanding atom chip experiments All the theory of laser ... Mirror-MagnetoOptical Trap (MMOT) and Magnetic Trap (MT), to cool and store the atoms 2.1 Laser cooling and trapping The laser cooling and trapping rely on the interaction of the atoms with the laser light field ... List of Figures 2.1 The optical pumping process preventing cycling cooling transitions in 87 Rb and use of a repumping laser to allow many absorption-emission cycles, required for laser cooling...
- 170
- 846
- 0
Saturable absorption and two photon absorption in graphene
... via quantum interference between photocurrents generated by one -photon and two- photon interband transitions In order to explore the two- photon absorption properties of the monolayer and bilayer ... comparing with monolayer graphene The study of saturable absorption and two- photon absorption of graphene will facilitate the application of graphene in generating ultrashort laser pulses and injection ... chirality of (10,10) Figure 1.12 The photo-excited carrier dynamics in graphene (a) Interband and intraband transitions of graphene under laser irradiance (b) Due to carrier-carrier and x List of Figures...
- 189
- 376
- 0
Aggregation enhanced two photon excited photoluminescence of noble metal nanoparticles and their chemical and biological applications
... enhancement of two- photon photoluminescence of noble metal nanoparticles Firstly, two- photon excited photoluminescence and two- photon induced singlet oxygen generation of Au nanospheres and two ratios of ... selective interactions between thrombin and a DNA aptamer, which induce aggregation of Ag NPs and result in significantly enhanced two- photon photoluminescence The LOD of our two- photon sensing assay ... based sensing and non cross-linking aggregation based sensing Aggregation of Au nanoparticles in sensors can be induced by an inter-particle cross-linking in which the enthalpic benefits of inter-particle...
- 145
- 346
- 0
Direct observations of nickel silicide formation on (100) si and si0 75ge0 25 substrates using in situ transmission electron microscopy
... Step two and three 120 A.2 Calibration and indexing of SAED pattern in Figure 5.1 (b) 121 A.3 Indexing of SAED pattern in Figure 5.2 (b) 124 A.4 Indexing of SAED pattern in Figure ... A.5 Indexing of SAED pattern in Figure 5.4 (c) 128 A.6 Indexing of SAED pattern in Figure 5.15 (b) 131 v Summary Recent success in the growth technology of Si1-xGex epitaxial thin films ... A.2: Showing the comparison and analysis of the calculated d-spacings of the Ni rings with d-spacing from a standard JCPDS file 04-0850 125 Table A.3: Showing the comparison and analysis of the...
- 151
- 526
- 0
Báo cáo y học: "Segment-orientated analysis of two-dimensional strain and strain rate as assessed by velocity vector imaging in patients with acute myocardial infarction"
... Int J Med Sci 2011, ability by the deformation indices two- dimensional strain (2DS) and strain rate (SR), might be an alternative approach in clinical routine Tissue Doppler Imaging (TDI) and ... myocardial infarction by strain, sSR, dSR or S´ after AMI The main finding of this study is the significant difference of strain, SRe and tissue velocities between infarcted and non-infarcted segments ... 24-29 In principle, our findings are confirming previously published studies about infarct detection and size, who described significant differences of the radial and circumferential strain and...
- 8
- 683
- 0
INTRODUCTIONS TO THE TWO SECTORS: JVS AND SOES
... Training Training is a highly important motivator according to reps and managers at JVs In contrast, reps and managers in SOEs consider it of low importance Understanding the importance of training, ... procedures, selling methods and techniques, job descriptions, etc are clearly stated and explained In addition, explaining the manual is always the main part of initial training in these companies ... capability in doing business, satisfies them the need of improvement or self-actualisation Working experience is largely obtained from training of the company Moreover, JVs often combine training with...
- 50
- 353
- 0
Tài liệu ADC KRONE - Guide - FO - Laser Optimized Fiber better in Price, Bandwidth, and Distance ppt
... IEEE 802.3 and ANSI/TIA/EIA 568-B Following is a table of IEEE GbE and 10GbE standards with related fiber types and bandwidths/distances (see Table 1) As shown in Table 1, standard 62.5µm and 50µm ... thus increasing Page Laser- Optimized Fiber: Built for Price, Bandwidth, and Distance to Make the Most of Your Investment Fiber Type Gigabit Link @ 850nm Laser IEEE 802.3z 1000BASE-SX Gigabit Link ... carefully examine your network and evaluate the distances and bandwidths required now and in the future To maximize your investment, you must choose the correct type and grade of fiber to support...
- 4
- 399
- 0
Báo cáo khoa học: Two types of replication protein A in seed plants Characterization of their functions in vitro and in vivo ppt
... protein–protein interaction assay) (Fig 3C–E) In the two- hybrid assay, the interaction between OsRPA70a and OsRPA32 yielded about 20% of the relative activity of the OsRPA32 and OsRPA14 interaction ... experiment, the indicated proteins were incubated together in NaCl ⁄ Pi containing 250 mM NaCl at °C for h, and then incubated with Talon beads The amount of protein present in the fraction bound ... role, such as in DNA replication, in living cells In contrast, mutants of AtRPA70b were viable but showed high sensitivity to UV and MMS, suggesting involvement of AtRPA70b in the repair of damaged...
- 12
- 588
- 0
The Gentlemen and the Roughs: The Collision of Two Honor Codes in the American North
... white-collar work of the expanding middle class, roughs earned honor and status through the giving and receiving of pain, as they were either unable or unwilling to acquire it through the climbing of the ... had command over an enlisted group of harddrinking, oft-fighting, working-class roughs A clash in codes of honor arose You had one company of men sitting quietly at temperance society meetings or ... scripture study, and another going off to drink and wench and brawl, and then coming back to bust up the temperance meeting and pour mule urine on the teetotalers On the one hand you had 17-year-old...
- 14
- 365
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25