tumor targeted delivery of sparingly soluble anti cancer drugs with polymeric lipid core immunomicelles
nanotechnology for cancer therapy, 2007, p.810
... Kang Moo Huh, Tooru Ooya, and Kinam Park Chapter 20 Tumor- Targeted Delivery of Sparingly- Soluble Anti- Cancer Drugs with Polymeric Lipid- Core Immunomicelles 409 Vladimir P Torchilin ... treatment of cancer Among the various approaches considered, nanotechnology offers the best promise for targeted delivery of drugs and genes to the tumor site and alleviation of the side effects of ... Polymeric nanoparticles are also mutable for enhancement of retention at the local tumor site and to combine anti -tumor agents with antiangiogenesis strategies.24–26 In addition to delivery of...
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Báo cáo khoa học: Protein transport in chloroplasts ) targeting to the intermembrane space pptx
... processed even in the absence of exogenous ATP The yield of pTic22 import in the absence of ATP varied between 20–50% of that in the presence of ATP The import of pMGD1 was efficient only at FEBS ... shown of the influence of thermolysin pretreatment of chloroplasts on the import of pTic22, pMGD1 and pSSU in the presence of ATP, derived by 2D densitometry evaluation (AIDA image analyser) of five ... the import of pTic22 and pMGD1 is much slower than that of pSSU, which makes the competition of pSSU import more visible due to the greater difference of the import rate with and without competitor,...
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Báo cáo khóa học: Fidelity of targeting to chloroplasts is not affected by removal of the phosphorylation site from the transit peptide doc
... proteins (A) Incubation of the translation product of GFP (27 kDa), and with isolated pea chloroplasts (+ Cp) (B) Incubation of the 33 kDa precursor of pTSSU.tp.wt-GFP (Twt), and with isolated chloroplasts ... location of targeted proteins in living tissue avoids the potential artefacts of in vitro systems, in particular their lack of specificity Furthermore, the stability of GFP ensures that problems of ... eight scans of the depth of a whole tobacco guard cell (A) GFP alone (B) pTSSU.tp.wt-GFP The GFP is clearly targeted to the chloroplasts as the GFP fluorescence overlays precisely with that of the...
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Báo cáo Y học: The C-terminal domain of perfringolysin O is an essential cholesterol-binding unit targeting to cholesterol-rich microdomains pptx
... activity of each toxin fragment was examined on TLC plates as described previously [16] Toxin fragments bound to the TLC plates were detected with antiwhole h-toxin antibody Preparation of lipid ... anti- C-terminal peptide antibody For measurement of binding to cultured cells, MOLT-4 (105 cells) [21] were washed twice with phosphate-buffered saline (NaCl/Pi), and then incubated with or without ... Measurement of Trp fluorescence A 0.3 nmol sample of each purified toxin fragment was mixed with either phospholipid-liposomes [toxin fragment/ phospholipids : 30 (mol/mol)] or cholesterol/phospholipid-liposomes...
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drug targeting, strategies, principles, and applications
... Analysis of Antibody Activity The antibody activity of the IT is compared to that of the free antibody and the activity of the IT is therefore expressed as a percentage of the activity of the free antibody ... Construction of Immunotoxins 21 Fig 12 Evaluation of the in vitro cytotoxicity of ITs ment of the antitumor effect following treatment with ITs Mice injected with tumor cells are treated with ITs ... Vitetta, E S (1992) The antitumor activity of an anti- CD22 immunotoxin in SCID mice with disseminated Daudi lymphoma is enhanced by either an anti- CD19 antibody or an anti- CD 19 immunotoxin Blood...
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Nanosized magnetofluorescent fe3o4–curcumin conjugate for multimodal monitoring and drug targeting
... biodegradable polymer with a high content of amino groups (–NH2 ) that makes possible complexation reaction with metal ions in solution and other chemical reactions with the purpose of improving polymeric ... solution (with Fe3+ /Fe2+ ratio of 2:1 Then, Cur (preliminarily solubilized in ethanol) was attached by adsorption on the Fe3 O4 surface of OLF/CSF Thus, several types of ferrofluid without /with Curcumin ... −20 to 20 kOe at 25 ◦ C, with accuracy of 10−5 emu The images of mice tumor were carried out by Philips Intera 1.5 T MR scanner (Netherlands) with the slice thickness of mm on transversal and...
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Báo cáo y học: "Nonimmunoglobulin E–Mediated Immune Reactions to Foods" pptx
... median of hours after the ingestion of food Symptoms typically start with emesis that often is followed by diarrhea.19 As with IgEmediated reactions, cow’s milk and soy proteins are the antigens ... and high levels of specific IgE The clinical removal of the allergens that react with the specific IgE of the patient can decrease AD symptoms in the affected patient.14 The role of T cells is ... the offending food on skin and/or nonreactive food-specific IgE tests As opposed to the immediateness of IgE-mediated reactions, the onset of the symptoms of FPIES is delayed from to 10 hours, with...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
... overexpression of Bcl-xL was correlated with prognosis of colorectal cancer (CRC) patients The aim of this study was to investigate the association of Bcl-xL expression with invasion and radiosensitivity of ... expression of Bcl-xL protein in tissues of LoVo tumors treated with PBS, Ad/shcontrol or Ad/shBcl-xL at day or 42 C Proliferation of tumors in the mice injected with LoVo cells treated with PBS, ... integral part of the preoperative treatment of rectal cancers However, only a minority of patients achieve a complete pathologic response to therapy because 13 of radioresistance of these tumors [27]...
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Báo cáo y học: "CD134 as target for specific drug delivery to T cells in adjuvant arthritis" pps
... Subsequently, cells were stained with anti- CD4-FITC, anti- CD134-biotin/streptavidin-PE and anti- TCR-αβ-PerCP, or with anti- CD45RA-FITC, anti- CD134biotin/streptavidin-PE and anti- TCR-αβ-PerCP Cell-associated ... in 5% CO2 without or with nmol (lipid) of the different liposomal formulations per well or with an equal concentration (100 nM) of free FUdR (Sigma-Aldrich Chemie) in 200 µl of medium without serum ... due to the binding of anti- CD134 (filled) or isotype control mAb (open) Representative stainings of one rat of three are shown (c) PLN cells were stained with anti- TCR-αβ and anti- CD45RA (rat B...
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Báo cáo y học: "Magnetically retainable microparticles for drug delivery to the joint: efficacy studies in an antigen-induced arthritis model in mice" doc
... All of the groups presented an adequate immune response of the levels of anti- mBSA antibody in the serum compared with an AIA-positive control mouse, with values generally superior to 80% of the ... treatment of arthritis, the articular retention of magnetic microparticles of a mean of and 10 μm in diameter with and without a magnet was compared by means of in vivo imaging For this long-term study, ... compliance with Swiss federal law on the protection of animals and in accordance with a protocol approved by the animal ethical committee of the Geneva University School of Medicine and the canton of...
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báo cáo khoa học: "Reactive oxygen species-mediated apoptosis contributes to chemosensitization effect of saikosaponins on cisplatin-induced cytotoxicity in cancer cells" pdf
... modulation of redox status in cancer cells The combination of saikosaponins and cisplatin could greatly improve the sensitivity of cancer cells to cisplatin Combination with agents that sensitize cancer ... various cancer cells (5-9) However, the effect of combination of these saikosaponins with chemotherapeutic drugs has never been investigated We addressed this question by treating a cervical cancer ... MCF-7 cancer cells The American journal of Chinese medicine 2003, 31(3):363-77 Motoo Y, Sawabu N: Antitumor effects of saikosaponins, baicalin and baicalein on human hepatoma cell lines Cancer...
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báo cáo khoa học: " The Washington Needle Depot: fitting healthcare to injection drug users rather than injection drug users to healthcare: moving from a syringe exchange to syringe distribution model" pps
... to the Office of National Drug Control Policy Letter to Office of National Drug Control Policy from Ranking Member Henry A Waxman on behalf of the Congress of the United States House of Representatives ... instance Policy makers and professionals are often complicit in all of this, insisting on an exchange to somehow make addicts accountable and forcing points of contact with professionals who sometimes ... retrieval at the point of contact with IDUs The WND makes use of the experience of active addicts, through paid employment, to provide an extremely vulnerable population of people with clean syringes...
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Báo cáo sinh học: " Anti-metastatic effects of viral and non-viral mediated Nk4 delivery to tumours" doc
... using Lipofectamine2000 (Invitrogen Corp., Paisley, Scotland) at day 7-post tumour induction The transfectability of LLC with lipofectamine 2000 was demonstrated in vitro with pEGFP-F delivery ... gene therapy of LLC tumours Plasmid Plasmid mediated Nk4 gene therapy of LLC tumours (a) Nk4 plasmid expression in vitro LLC cells were transfected with pSelectBlasti-2BhIL32b by lipofection in ... generated by cloning of the Nco1, Xba1 fragment of pGL3 (Promega) containing the firefly luciferase gene, by blunt end ligation in the EcoRI, Xba1 region downstream of the CMV promoter of AAV-MCS cloning...
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Báo cáo y học: "Custom astrocyte-mediated vasomotor responses to neuronal energy demand" pptx
... PGT-mediated exchange of intracellular lactate for extracellular PGE2 These findings establish lactate as a novel operator of the astrocyte vasomotor switch and are consistent with the ability of lactate ... opposite pro-dilatory effect of NO has also been observed owing to NO-mediated inhibition of CYP P450 4A and 20HETE production [13] and it is not yet clear how these effects of NO at CYP P450s are ... that vasodilation dominated near the low end of physiological PO2 in brain slices Determination of where the switch point for dilation is may rest with characterizing how low PO2 must fall before...
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Advanced control strategies for automatic drug delivery to regulate anesthesia during surgery
... doses of the drugs to tightly maintain 18 Chapter Introduction the variables at desired set-point Also, with automatic controllers, the degree of drug interaction of different drugs can be quantified ... compared to most other drugs used for the rapid intravenous induction of anesthesia This is one of the most important advantages of propofol compared to other intravenous anesthetic drugs Inhalation ... objective of this work is to develop the MPC control strategies for regulation of hypnosis with various drugs and thoroughly evaluate and compare MPC controller’s performance with the performance of...
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Báo cáo sinh học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" potx
... BacFLT-GFP with increasing concentrations of butyrate or TSA (c) Fold induction of the expression of GFP in transfected cells with the transfer plasmid pBlueFLT-GFP (see Methods) and treated with increasing ... images of retinas injected with vehicle (h) or with BacFLT-GFP (i) In red is shown the localization of von Willebrand factor (red) within characteristic blood vessel structures The colocalization of ... interfere with the process of DNA methylation, was added to a concentration of 25 µM alone or in combination with butyrate or TSA We did not observe a significant increase of transgene expression with...
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Báo cáo hóa học: " In vivo transcriptional targeting into the retinal vasculature using recombinant baculovirus carrying the human flt-1 promoter" docx
... BacFLT-GFP with increasing concentrations of butyrate or TSA (c) Fold induction of the expression of GFP in transfected cells with the transfer plasmid pBlueFLT-GFP (see Methods) and treated with increasing ... images of retinas injected with vehicle (h) or with BacFLT-GFP (i) In red is shown the localization of von Willebrand factor (red) within characteristic blood vessel structures The colocalization of ... interfere with the process of DNA methylation, was added to a concentration of 25 µM alone or in combination with butyrate or TSA We did not observe a significant increase of transgene expression with...
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Báo cáo y học: "Blood autoantibody and cytokine profiles predict response to anti-tumor necrosis factor therapy in rheumatoid arthriti" pps
... development of antigen microarrays and application of these arrays to characterize autoantibody phenotypes associated with a variety of autoimmune diseases [13] We further developed RA antigen microarrays, ... of elevated blood levels of the proinflammatory cytokines TNF, IL-1β, IL-6, IL-13, IL-15 and granulocyte- macrophage colony-stimulating factor with autoantibody targeting of citrullulinated antigens ... independent cohorts of patients with RA The workflow of the studies is outlined in Figure Materials and methods Patient sera Pretreatment sera from three cohorts of patients with the diagnosis of RA based...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... binding of cAMP to the anti- cAMP-binding sites of the protein The distance between the sulfur atom of Cys178 and the C9C10 bond of the indole ring of Trp85, derived from the crystal structure of CRP(cAMP)2 ... the mobility of helix A of the protein As Trp13 is located in the vicinity of the activation region AR2 of the protein [1], which is responsible for the activation of the second class of E coli ... correlation time of Trp85 in apo-CRP of 20.5 ns indicates that this residue is immobilized within the N-terminal domain of the protein and exhibits motion characteristic of the whole protein (within experimental...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
... blotted with antiphospho-ACC Ig or antiphospho-AMPK primary Ig Following measurement of phosphorylation of Thr172 within AMPK a-subunits (P-T172) blots were stripped to measure total abundance of ... palmitate These effects of palmitate were seen with or without AMP in the assays (Table 1) Perfusion with 0.5 mM oleate caused activation of both AMPKs to levels similar to those seen with 0.5 mM palmitate ... for comparison of 60 with 20 values chargeÕ in control compared with that in control (Table 3) Time-dependence of the activation of AMPK by fatty acids No significant activation of AMPKs by 0.5...
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