trx1 trx2 and txnip in the retina after ont and iop elevation and in rgc 5 cells with induced oxidative stress
Báo cáo khoa học: Photodynamic treatment and H2O2-induced oxidative stress result in different patterns of cellular protein oxidation ppt
... to determine the sites of the oxidative modifications within the tubulin molecule, and to elucidate the role of these modifications in the functional damage to tubulin Interestingly, for the two ... shown in (A) and (B) Silver staining is shown in (C) and (D) in blue superimposed with the spots of oxidized proteins shown in red Protein labels: 1, a-tubulin; 2, b-tubulin; 3, actin; 4, PDI; 5, ... crosslinking of proteins Kinetics of the FMN- 24 25 26 27 28 29 30 31 32 33 34 35 36 and rose bengal-sensitized photooxidation and intermolecular crosslinking of model tyrosine-containing N-(2-hydroxypropyl)...
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Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential doc
... Cyclosporin A -induced oxidative stress M van der Toorn et al calcineurin therefore play a role in cyclosporin A -induced oxidative stress and disturbed Ca2+ homeostasis Immunosuppressive treatment with ... those induced by CsA All three compounds induced a comparable degree in ROS production in cells devoid of P-glycoprotein Unlike CsA, both NIM811 and PSC833 not inhibit calcineurin Together, these ... versus the consistency in the effects of CsA and NIM811 in these experiments suggests that MPTP blockage plays a role neither in the observed increases in cytosolic [Ca2+] nor in increases in ROS...
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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
... study of PM2 .5 - and PM10 - induced oxidative stress in rat lung epithelial cells 15 Fig RT-PCR analysis of catalase mRNA The level of catalase mRNA induced by PM2 .5 was decreased with concentrationdependent ... Cytotoxicity and induction of proinflammatory cytokines from human monocytes exposed to fine(PM2 .5) and coarse particles(PM10-2 .5) in outdoor and indoor air Toxicol Appl Pharmacol 1999, 155 , 2 45- 252 20 ... collected onto two separate filters: one filter containing PM10 and the other filter containing PM2 .5 Preparation and extraction of filters Teflon filters were baked for h at 100oC and transferred into...
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Analysis of transcription factors in living human cells with the help of split ubiquitin system
... procedure involves fusion of the TAP tag to the target protein and introduction of the construct into the host cells Cell extracts are prepared and the fusion protein along with the interacting partners ... Linker Histone and 57 Core Histone Proteins inside Human Cells using the Split-Ubiquitin System 2.1.1 Cloning 57 2.1.1.1 PCR, Restriction and Ligation 57 2.1.1.2 Transformation of DH5 cells 58 ... 75 human tissues, significant enrichment in one or more tissues was seen for 59 of the 69 (86%) known motifs and 53 of the 1 05 (50 %) new motifs The TATA Box is the binding site for the TATA-binding...
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Báo cáo khoa học: Fasting-induced oxidative stress in very long chain acyl-CoA dehydrogenase-deficient mice pdf
... significantly increased from 53 .56 ± 5. 3 to 78 .58 ± 5. 5 UÆmg)1 in WT mice and from 48.29 ± 5. 2 to 147.43 ± 20.4 UÆmg)1 in VLCAD KO mice after fasting (Fig 4) Of concern was the fact that the MCT diet increased ... activity to 70. 95 ± 4.4 UÆmg)1 in WT mice and 91 .55 ± 8 .5 UÆmg)1 in VLCAD KO mice in the nonfasted state Interestingly, the MCT diet combined with fasting significantly reduced GPX activity in WT mice, ... of the dry weight in the WT mice and 26.4 ± 3.1% in the VLCAD KO mice – under nonfasting conditions, and these percentages increased further with fasting (Fig 1) In parallel with liver lipids,...
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Báo cáo y học: "EGb761 protects motoneurons against avulsion-induced oxidative stress in rats Research article" ppsx
... levels in the serum and injured spinal cord at the d, w, w, and w time points Groups 9-16 were used for determination of cNOS and iNOS levels in injured spinal cord at the d, w, w, and w time points ... 0. 05 Results Effect of EGb761 on NO levels in the serum and injured spinal cord Following spinal root avulsion, the levels of NO in the serum and spinal cord increased, reaching a maximum at w and ... cNOS, iNOS activities in the injured spinal cord and nNOS expression in injured motoneurons Competing interests The authors declare that they have no competing interests Authors' contributions The...
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Báo cáo khoa học: "Impaired antiviral activity of interferon alpha against hepatitis C virus 2a in Huh-7 cells with a defective Jak-Stat pathway" pdf
... positive cells in the culture was determined The dilution of virus-containing supernatant that showed 50 % GFP positive cells 96 hours after infection in the culture (called the TCID50) was determined ... recombinant DNA and cloning was performed in four different steps In the first step, the 228 bp (F1) recombinant DNA fragment containing 70 amino acids of NS5A (nts.7339 754 6) fusion with the first ... pEGFP-N1 plasmid and inserted in- frame of the NS5A coding sequence of the JFH1 cDNA clone between 2394 and 23 95 amino acids position (between 417 and 418 amino acids of the NS5A protein) The PCR amplification...
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Sensibility study of flooding and drying issues to the operating conditions in PEM fuel cells
... model are the fluxes exchanged between the channel and the GDLs as in well as the channel inlet conditions ( SR in , RH in , Ptot , y in ) which give Qiin and yiin ISSN 2076-28 95 (Print), ISSN ... received the B.Sc and M.Sc degrees in mathematics from the University of Lome, Lome, Togo, in 1998 and 1999, respectively, and the M.Sc and Ph.D degrees in industrial engineering from the University ... of the PEMFC [2] Accurate water content is therefore required in the cell In this context, numerical models combined with experimentation can help to understand the mechanisms involved in the...
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Tài liệu Báo cáo khoa học: Pyruvate reduces DNA damage during hypoxia and after reoxygenation in hepatocellular carcinoma cells pptx
... from 75 to 300 lm hydrogen peroxide At 240 lm exposure and after normalization to a control without any cells (100%), the levels of ROS were 71% and 54 % for cells incubated without and with pyruvate, ... maximum in both the absence and the presence of pyruvate, and thereafter decreased The increase induced by h of reoxygenation was lower in the presence of 0.8 mm pyruvate, although 40 35 DNA fragmentation ... decreases the level of ROS in the medium of HepG2 cells exposed to hydrogen peroxide HepG2 cells were incubated with 5. 5 mM glucose in the presence of 0.8 mM pyruvate, and exposed to increasing doses...
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Tài liệu Báo cáo khoa học: Oxidative stress in the hippocampus after pilocarpineinduced status epilepticus in Wistar rats doc
... significant increase in hippocampal nitrite content of 51 % [T(18) ¼ 25. 959 ; P < 0.0001] compared with the control group On the other hand, a 55 % decrease in GSH concentration [T(10) ¼ 27. 452 ; P < ... [UÆ(mg protein))1] and catalase [mmolÆmin)1Æ(lg protein))1] activities in the hippocampus of adult rats after pilocarpine -induced SE Male rats ( 250 –280 g, months old) were treated with a single dose ... related to the mechanisms involved in the initiation and ⁄ or propagation of FEBS Journal 272 (20 05) 1307–1312 ª 20 05 FEBS seizures induced by pilocarpine Our results are in agreement with another...
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Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... with the most prominent band at 60 kDa (lane 1) In the 27-month-old preparations (lane 2) only eight main bands are stained, with two intense signals of 70 and 50 kDa, while the bands at 60 and ... with aging (Eur J Biochem 270) 2299 Western blotting of modified proteins Fig Determination of CML-protein content in liver mitochondrial matrix with aging The CML content in the matrix proteins ... Protease activity in the absence of ATP was lower in aging rats than in adults Activity decreased by 51 % between 10-monthold and 27-month-old rats (1. 15 ± 0. 15 and 0 .59 ± 0.08 FUÆlg protein)1Æh)1, respectively)...
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Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf
... galA and by then cloning a fragment, starting at this NsiI site and containing the galA gene and approximately 700 bp 3¢-flanking region, in pIM4700 (containing the pkiA promoter) A niger The sugar ... above with a pH range of 3 .5 7 .5 The specific activity of GALA was determined using GALACTAZYME tablets (Megazyme) with conditions as indicated by the supplier and using 0, 10, 25, 50 , 100, 200 and ... g) of wet mycelium were then transferred to 50 mL minimal medium containing carbon sources as indicated in the text After h of incubation in a rotary shaker at 250 r.p.m and 30 °C mycelium was...
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Tài liệu Báo cáo khoa học: Cell surface heparan sulfate proteoglycans Target and partners of the basic ®broblast growth factor in rat Sertoli cells pptx
... sulfation on proteins and carbohydrate residues in intact cells without inhibiting cell growth or protein synthesis [ 35 37], and proteoglycan sulfation in cultured Sertoli cells from 20-day-old ... age The bFGF binding requires speci®c motifs on highly sulfated HS chains Sodium chlorate, in inducing structural alteration of HS chains, prevents the bFGF binding Our results indicate that the ... occuring during Sertoli cell development [56 ] In this way, syndecan-1 and syndecan-4 could participate to this event in reorganizing actin ®laments via their cytoplasmic domain [57 ] but also in the...
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Tài liệu Báo cáo Y học: Presence and regulation of the endocannabinoid system in human dendritic cells potx
... against a sequence between the N-terminal and the first transmembrane domain of the protein of the human and rat CB2 receptor The specificity of the CB1 and CB2 antibodies was described in McIntosh ... mononuclear cells (monocytes and lymphocytes) and a lower band containing polymorphonuclear leukocytes (granulocytes) and the red cells The PBMC were recovered, washed with RPMI and counted After a further ... endocannabinoid system, we investigated the presence and regulation of endocannabinoids, cannabinoid receptors and FAAH in immature and mature dendritic cells obtained by stimulation with either the...
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Báo cáo khoa học: Muramyl-dipeptide-induced mitochondrial proton leak in macrophages is associated with upregulation of uncoupling protein 2 and the production of reactive oxygen and reactive nitrogen species docx
... NaCl, 25 mm CaCl2) The solution (100 lL, · 1 05 cells) was transferred to a mL culture tube containing lL of FITC Annexin V and lL propidium iodide The cells were gently mixed and incubated for 15 ... effects on the UCP2 modulates MDP -induced mitochondrial inefficiency central nervous system, and the lack of induction of in ammatory responses [22] Despite a long-standing interest in the field ... by correlating the uncoupling effect induced by these molecules with the level and function of UCP2 and free radical production in macrophages We find that MDP induces reactive oxygen and nitrogen...
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Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... corresponding to Bcp2 were detected in noninduced and induced cells The increased amount of Bcp2 in the cytoplasmic fraction of S solfataricus correlated with increased bcp2 mRNA level after treatment ... processes In this study we examined the role of Bcp2 in order to increase the knowledge of the enzymatic activity involved in the oxidative stress in S solfataricus To detect differences in the response ... to the cultures inhibits growth without killing the cells The hybridizing band in the northern analysis showed the expected size of about 680 bp indicating that the gene is transcribed as a monocistronic...
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Visitor Profiles, Economic Impacts and Recreational Aesthetic Values Associated with Eight Priority Florida Springs Located in the St. Johns River Water Management District pot
... Spending $211 .55 $1 15. 81 $192.47 $ 255 .69 $7 75. 52 Wages Created $ 45. 14 $26.21 $43.14 $55 . 75 $170.24 14.47 8.19 13 .58 18.16 13.60 $21.99 $30.67 $52 .86 $56 .43 $19.28 $5. 53 $21 .53 $3.27 $24.04 $ 15. 55 ... spending, wages, and employment generated by them Tables A.3.1 to A.3.8 (Appendix A) contain the worksheets for the four quarters of the year beginning in the 3rd quarter of 2003 and ending with the ... located in the northern part of Central Florida and contains three priority springs: Alexander, Apopka, and Bugg Spring Alexander Springs is located in the Ocala National Forest and is owned and...
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Accelerating reproductive and child health programme impact with community-based services: the Navrongo experiment in Ghana pptx
... providing them with n a start-up kit of essential drugs and cond ducting training in managing services and revolving accounts so that the flow of supplies would be sustainable and financed by the ... mortality Reduci n ing fertility depended upon combining the presence of nurses with community mobilization and the involvement of men in family planning These findings attest to the demographic ... versus 224 to 100 per thousand person-years in the combined experimental area 19 95 200 2001 150 100 50 15 19 20—24 25 29 30—34 35 39 40—44 45 49 Age group Bulletin of the World Health Organization...
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Báo cáo khoa học: Yeast oxidative stress response Influences of cytosolic thioredoxin peroxidase I and of the mitochondrial functional state pot
... treated during 40 with no agent as a control or with 1.2 mM H2O2 and 0.1 lgÆmL)1 antimycin A (anti A), singly or in association, as indicated in the figure The symbols –, + or ± in the last column ... accumulation induced by H2O2, in neither the wild-type nor in tsa1D cells (Fig 6) Hence, the dimin- ished H2O2 -induced expression levels of some Yap1target genes observed in cells with impaired ... accessibility of Yap1 to the GSH1 promoter could be repressed by Cbf1, a DNA-binding protein that binds to elements in the vicinity of Yap1 binding site [52 ] Alternatively, the increased reducing power of...
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Báo cáo khoa học: Alternative initiation of transcription of the humanpresenilin 1gene in SH-SY5Y and SK-N-SH cells The role of Ets factors in the regulation ofpresenilin 1 pptx
... tenfold in SK-N-SH and cells Further deletion to +42 increased transcription in both cells lines: about fivefold in SH-SY5Y cells and twofold in SK-N-SH cells Further deletions to +6 did not result in ... activity indicating the crucial importance of these two Ets motifs Mutations at + 65 and +129 resulted in a mild 25 30% decrease in SH-SY5Y cells and 40 50 % decrease in SK-N-SH cells The double ... all contain the highly conserved ETS domain ERM contains two DNA binding inhibitory domains flanking the Ets domain: the central 87 amino acid inhibitory domain is poorly conserved and virtually...
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