trp53 p16ink4a p19arf gene deletion on the frequency of murine hematopoietic stem cells
Effects of combined mechanical and pulsed electromagnetic field stimulations on the osteogenesis of bone marrow stem cells
... forms the dense outer shell of most bones and it accounts for 80% of the total bone mass Cortical bone is mostly found in the shaft portion of long bones and the outer shell found around the spongy ... cancellous bone On the other hand, cancellous bone consists of a network of struts surrounding interconnected spaces It is the spongy interior bone tissue which makes up 20% of the total bone mass ... bone by lamellar bone and the resorption of excess callus Gradual modification of the fracture region leads to restoration of normal bone architecture Figure 1.1: Schematic representation of the...
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Tài liệu Báo cáo khoa học: Effect of deletion of the DNase I hypersensitive sites on the transcription of chicken Ig-b gene and on the maintenance of active chromatin state in the Ig-b locus docx
... mRNA in cells with a deletion in region II (Fig 4A) demonstrated the essential role of this region in the transcription of the Ig-b gene Deletion of region III decreased the expression of Ig-b ... the proliferation of DT40 cells (data not shown), and therefore deletion of region IV was carried out by the introduction of the region IV deletion construct into 16 kb Del cells Genomic Southern ... state of the cells with any deletion in the regions I–IV in the Ig-b locus, judged by DHSs as the indicator, was the same as that in predeletion cells, the region required for the maintenance of the...
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Báo cáo y học: "Influence of sequence identity and unique breakpoints on the frequency of intersubtype HIV-1 recombination" pot
... shares a region of homology with an acceptor RNA template upstream of a genetic marker (lacZ') on the acceptor RNA or a truncated, nonfunctional portion of the malT gene from E.coli on the donor template ... Increased frequency of recombination in vitro can be misleading considering the inability to reconstitute the conditions of endogenous HIV-1 reverse transcription Compositions of buffers, concentrations ... recombination in the conserved regions of the env genes of multiple HIV-1 subtypes However, only the appropriate sequence combination can generate similar hairpins in a hypervariable region of the donor...
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Báo cáo khóa học: CD38 is expressed as noncovalently associated homodimers on the surface of murine B lymphocytes doc
... dependent on the formation of interdisulphide bonds between CD38 monomers, indicating that the CD38 homodimers in B cells are likely to be stabilized via noncovalent monomer– monomer interactions Finally, ... FACSCalibur (Becton-Dickinson, San Jose, CA, USA) Surface biotinylation of proteins To analyze the stability of CD38 on the plasma membrane of the different Ba/F3 mutants, labeling of the surface ... homodimers (data not shown) These results suggest that the stabilization of CD38 homodimers by crosslinkers depends strongly on the conformation and orientation between the CD38 monomers It has been...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... level of non-target genes of the same number was also correlated with the miRNA expression Density plot of correlation coefficient distribution was generated in R environment Validation of differentially ... coefficients of miRNA-target gene pairs The expression of miR-302c and miR-502b and their predicted target genes was analyzed by correlation analysis The distribution of the correlation coefficients ... Identification of hundreds of conserved and nonconserved human microRNAs Nat Genet 2005, 37(7):766-770 Liang Y, Ridzon D, Wong L, Chen C: Characterization of microRNA expression profiles in normal...
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Effect of Na2SO4 additive in positive electrodes on the performance of sealed lead-acid cells for electric scooter applications ppt
... the performance of VRLA cells Based on the results presented herein, we can conclude the following Ž1 The XRD analyses showed that the major constituent of the additive Na SO4 in the cured plates ... work, we studied the effect of Na SO4 additive in the positive electrode on the performance of VRLA cells for ES applications Experimental procedures 2.1 Cell construction Each cell contained two ... the paste was - wt.% The current density was controlled at mA cmy2 and the formation capacity had a theoretical capacity of about 200% under the formation After formation, the plates were washed...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... by the addition of inhibitors of these enzymes - GW274150 and indomethacin [8,36], respectively - to the co-cultures The addition of these inhibitors resulted in the abrogation of the inhibition ... CIA induction and evaluation and to analysis of T-cell proliferation PM contributed to the design of the study and to manuscript preparation All authors contributed to interpretation of the data ... μl of PBS in the left ear DTH response was calculated as the percentage swelling (the difference between the increase of thickness of the right ear and the left ear, divided by the thickness of...
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Tissue engineering approaches to tendon repair studies on the use of bone marrow stromal cells and knitted poly (d, l lactide co glycolide scaffold
... the calcaneus bone As organs, tendons consist of three parts: the muscle attachment region, the substance of the tendon itself, and tendon to bone insertion region These three parts vary in their ... and the inorganic components Collagen constitutes approximately 90% of the total protein of the tendons or 65-75% of the dry mass of tendons Elastin accounts for only about % of the dry mass of ... tendons of the hand Following a full transection, there is a spontaneous retraction of the cut tendon ends In the Achilles tendon of both a rat and rabbit model, the gap formed by the retraction...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation of erythroid cells from murine ES cells ... and was only detected at the core regions [14,15] Localization of RNA Pol II to the LCR was independent of active transcription elongation; the addition of the elongation inhibitor DRB did not ... able to generate cells of both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... activations that lead to the binding of downstream transcription factors, e.g., Gli1, Gli2 and Gli3 to their target sequences and then expression of target genes involved in the control of cell ... activation of basal/ stem cells and their progression toward a metastatic status under the influence of Hh signalling remain to be further elucidated To further characterize the basal cells during ... entire process of prostate tumorigenesis was reconstituted in vivo and the effects of Hh overexpression on the normal prostate stem cells was shown by the transformation of P63+/Patch1(Low or -) and...
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Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"
... contamination PCR reaction triplicates showed no major differences among them and expression levels of the internal control were similar in the different experiments The expression levels of csp gene ... deviation of the mean No amplification was observed when cDNA controls, that lack the reverse transcriptase enzyme, were used in the real time PCR reactions, demonstrating that there was no gDNA contamination ... Quantification relies on the comparison of the critical threshold cycle (Ct) of an unknown sample against a standard curve of known quantities Ct is the amplification cycle at which the fluorescence...
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Báo cáo hóa học: " Research Article Effects of Carrier Frequency Offset, Timing Offset, and Channel Spread Factor on the Performance of Hexagonal Multicarrier Modulation Systems" doc
... usually depends on the precision of carrier frequency offset estimation and symbol timing A generalized framework for the prediction of OFDM system performance in the presence of carrier frequency offset ... two-dimensional (2D) case Specifically, in OFDM system with symbol period T and subcarrier separation F, the transmission functions of OFDM system consist of translations and modulations of a single ... channel dispersion point of view Synchronization is considered as a key factor of designing multicarrier modulation system receiver The synchronization precision significantly affects the receiver...
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Báo cáo hóa học: " On the Compensation of Delay in the Discrete Frequency Domain" pot
... satisfy these properties, then such an expression is only approximate To facilitate the application of the general FIR filter analysis of Section 3, let the signal s(n) pass through the transmission ... Reconstruction SNR for filterbank FDAF equalisation of constant delay Figure 8: Reconstruction SNR for filterbank FDAF equalisation of the linear delay channel The solid trace of Figure shows the ... applications, this may be the most appropriate measure of quality In others, including conventional equalisers and That is, the number of bins in the frequency domain implementation is equal to the...
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Báo cáo lâm nghiệp: "Improving RBS estimates – effects of the auxiliary variable, stratification of the crown, and deletion of segments on the precision of estimate" pps
... are presented The analysis of the effects of the crown structure concentrates on the stratification of the crown and on the deletion of greater segments (e.g the stem) by using the classical ... reduces to 6.7% after deletion of the stem segments After this closer look at the old pine, the effect of stratification and deletion of the stem segments on the precision of estimates is to be ... and the unconditional probability Qs of the segment If, for example, fr is measured at the rth segment of the path, then fr/Qr is the contribution of this segment to the estimate of the total of...
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Báo cáo khoa học: "Impact of the frequency of online verifications on the patient set-up accuracy and set-up margin" ppt
... deviations of all contributing systematic errors, s the square-root of the quadratic sum of the standard deviations of all contributing random errors, and sP the standard deviation describing the ... delineation errors were not considered in the calculation of the treatment margins because the focus of this study was the patient positioning set-up error The representative standard deviation of the ... Frequency of online verifications; 0% = No online verification; 20% = Online verification once a week; 50% = Online verification every other day scenarios and improved from no online verification to online...
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Báo cáo y học: "Identification of bacteria on the surface of clinically infected and non-infected prosthetic hip joints removed during revision arthroplasties by 16S rRNA gene sequencing and by microbiological culture" doc
... amplification of the 16S rRNA gene, a highly conserved region within the bacterial genome, is invaluable in the detection of the bacterial types involved in prosthetic hip joint infections [10,19,20] ... impact on improving the laboratory diagnosis of prosthetic hip joint infections Conclusion A wide range of bacteria can be found on the surface of prosthetic hip joints removed at revision arthroplasty ... culture of the same samples The clinical interest of this study is the presence of any organism on the prosthetic hip joints and the role, if any, that they have in initiating, pro- Page of 11...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... mean that the cells are synchronized Synchronized divisions are the sine qua non of synchrony Criteria for successful analysis of gene expression during the division cycle 12) Gene expression results ... G1-phase DNA contents Criteria for cell synchronization and analysis of gene expression 8) The size distribution of newly synchronized cells should be narrower than the size distribution of the original ... but the cells are all recombined together and analyzed These combined cells should not give a variable pattern of gene expression This controls for perturbation of the culture by the selection...
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Báo cáo y học: "A network perspective on the evolution of metabolism by gene duplication" pdf
... important source of metabolic variability and also of biochemical innovations Influence of distance between reactions on the retention of duplicates In addition to the retention of duplicates generating ... gene duplications and horizontal gene transfer, could enhance our understanding of the influence of metabolic versatility in the evolution of species interactions We therefore consider that gene ... that the majority of nodes show a low degree of connectivity and the topology of the network is dominated by a small fraction of highly connected nodes Another property of metabolic networks is their...
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Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 1
... INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY NATIONAL UNIVERSITY OF SINGAPORE 2011 INVESTIGATIONS ON THE ROLES OF UBIQUITIN IN THE REGULATION ... REGULATION OF HEAT SHOCK GENE HSP70B’ EE THIAM HEE, GARY (B SCI (HONS), NUS A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL ... DOCTOR OF PHILOSOPHY DEPARTMENT OF MICROBIOLOGY YONG LOO LIN SCHOOL OF MEDICINE NATIONAL UNIVERSITY OF SINGAPORE 2011 ...
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Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 2
... Regulation 40 1.5.4 The Proteolytic Role of the Proteosome in the Regulation of Transcription 42 1.5.4 The Non-Proteolytic Role of the Proteosome in the Regulation of Transcription 47 1.5.6 The Role ... outside of the context of proteosomal degradation (Hicke et al., 2005) Each of these modifications has their role in the regulation of transcription In this project we investigated the role of ubiquitin ... modification of transcription factors is one means by which the cell achieves transcriptional regulation Of particular interest to us is the ubiquitination of transcription factors Ubiquitin traditionally...
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