transgene free production of pluripotent stem cells using piggybac transposons
báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
... Genetic engineering of human pluripotent cells using TALE nucleases Nat Biotechnol 2011, 29:731-734 88 Zhao T, Zhang ZN, Rong Z, Xu Y: Immunogenicity of induced pluripotent stem cells Nature 2011, ... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human-induced pluripotent ... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells...
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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot
... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... excision of reprogramming factors Nature 2009, 458:771-775 Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin, II, Thomson JA: Human induced pluripotent stem cells free of vector and transgene ... Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent stem...
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Atlas of Human Pluripotent Stem Cells doc
... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... Morphology of Human Embryonic Stem Cells and Induced Pluripotent Stem Cells Cultured in Feeder Layer -Free Conditions 3.1 Introduction 3.2 Materials for Feeder Layer -Free Culture of ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies,...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... the cell type of origin used to derive iPSC (c) H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3;...
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Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...
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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... induce the cells to pluripotent cells These pluripotent cells are studied and characterized The pluripotent stem cells exhibited morphology and genomic markers of pluripotency These cells were ... Culture of Mouse Embryonic Fibroblast (MEF) cells as feeder cells for hESCs 37 4.2 Culture of Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on...
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Detection of breast cancer cells using targeted magnetic nanoparticles and ultra-sensitive magnetic field sensors potx
... and characterized In anticipation of the identification of new markers in the future and the possibility of using cocktails, we are focusing on the development of a universal probe, based on iron ... labeling of attached cells, cells were cultured on acid-washed glass coverslips Quantification of Her2 site density was performed as previously described by using approximately 105 cells and ... Mexico School of Medicine, MSC07 4025, University of New Mexico, Albuquerque, NM 87131, USA 3Department of Pathology, University of New Mexico School of Medicine, MSC08 46401 University of New Mexico,...
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Báo cáo khoa học: Epigenetics: the study of embryonic stem cells by restriction landmark genomic scanning pptx
... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... methylation profiles Epigenetic study of embryonic stem cells Differences in DNA methylation profiles between ES and other stem cells Comparing ES cells with other stem cells established from developing ... contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells (77)...
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differentiation of embryonic stem cells
... TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils 10 Development and Analysis of ... screening procedure is use of FCS samples for the production of MEDII and formation of EPL cells in adherent culture The methods for production of MEDII and formation of EPL cells are given elsewhere ... and Human Embryonic Stem Cells 29 Growth and Differentiation Monkey ES Cells of Cynomolgus 30 Isolation, Characterization, and Differentiation of Human Embryonic Stem Cells HIROFUMI SUEMORI NORIO...
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báo cáo hóa học:" Implantation of neural stem cells embedded in hyaluronic acid and collagen composite conduit promotes regeneration in a rabbit facial nerve injury model" doc
... immunohistochemical staining of BrdU and S-100 Representative images of immunohistochemical staining of BrdU and S-100 A: Localization of darkly brownish stained BrdU+ cells to transected tissues of rabbits having ... 7042014 of the National Science Foundation of Beijing, China, the 50573044 of the National Natural Science Foundation of China and the 2005CB623905 of the National Basic Research Program of China ... protruding processes Immunofluorescence staining of β-tubulin, GFAP and GalC demonstrated positive expressions in a substantial number of cells, suggesting that neurosphere-derived cells were able to...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... the event of autologous implantation, but induced pluripotent stem cells are at least, if not more capable than those from adult (stem) cells Mesenchymal stem cells Although adult stem cells are ... here lies in implantation of hepatic stem cells, or in implantation of hepatic cells obtained by differentiation of a different type of stem cell, such as mesenchymal stem cells Other applications,...
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báo cáo hóa học:" Targeting of mesenchymal stem cells to ovarian tumors via an artificial receptor" docx
... phenomenon of MSCs was confirmed in different experimental systems [2,12] Other cell types, such as umbilical cord matrix stem cells (UCMS) [14], neural stem cells [15,16] and endothelial progenitor cells ... delivery of CX3CL1 to multiple lung tumors by mesenchymal stem cells Stem Cells 2007, 25(7):1618-26 12 Komarova S, Kawakami Y, Stoff-Khalili MA, Curiel DT, Pereboeva L: Mesenchymal progenitor cells ... superimposed using the Adobe Photoshop 7.0 software Statistics on bioluminescent signal intensity was obtained using WinView software according to the software instruction For comparison of tumor...
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Báo cáo khoa học: "In vitro neuronal and osteogenic differentiation of mesenchymal stem cells from human umbilical cord blood" ppt
... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...
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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot
... accelerated degeneration of the treated IVD [18-24] An alternative cell source are adult stem cells, in particular, bone-marrow-derived mesenchymal stem cells (MSCs) The use of these cells would allow ... haematopoietic cells using RosetteSep (STEMCELL Technologies SARL, Grenoble, France) prior to isolation of mononuclear cells using a Histopaque 1077 gradient (Sigma, Poole, UK) Cells were cultured ... viewed using fluorescent microscopy to identify green fluorescent protein (GFP)-infected cells Following identification of the position of injection site and the presence of GFP-labelled cells, ...
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Báo cáo y học: "Immunomodulatory properties of mesenchymal stem cells: a review based on an interdisciplinary meeting held at the Kennedy Institute" potx
... mesenchymal stem cells and neural progenitor cells Haematologica 2003, 88:126-133 Reyes M, Verfaillie CM: Characterization of multipotent adult progenitor cells, a subpopulation of mesenchymal stem cells ... L-selectin)/lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts Augments B responses/ plasma cells Growth, differentiation, and isotype-switching of B cells/ B cells Ligand ... infused in the 32nd week of pregnancy After an uneventful pregnancy a bone biopsy performed at months of age demonstrated engraftment of Y chromosome positive cells (7.4% of total cells) [52] These...
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Báo cáo y học: "Microenvironmental changes during differentiation of mesenchymal stem cells towards chondrocytes" ppt
... Expression levels of ADAM and adhesion molecules during chondrogenic differentiation of mesenchymal stem cells (MSCs) The gene-expression (MSCs) profile of mesenchymal stem cells (MSCs; n = 2) ... 39 40 41 42 43 44 45 46 47 48 49 characterization of mesenchymal stem cells Stem Cells 2006, 24:475-481 Charo IF, Ransohoff RM: The many roles of chemokines and chemokine receptors in inflammation ... gene-expression profile of mesenchymal stem cells (MSCs; n = 2) was analysed by real-time PCR during their differentiation towards chondrocytes in micropellets (a) Time-course expression of the members of the...
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The signals of FGFs on the neurogenesis of embryonic stem cells ppsx
... detect the Sox1-GFP+ cells from day to day (Fig 3A) The number of Sox1+ cells became 20% of total cells on day and reached the plateau, 50% of total cells, on day Treatment of FGF1 consistently ... promotes self-renewal of mouse embryonic stem cells Dev Biol 1999, 210:30-43 Li Z, Theus MH, Wei L: Role of ERK 1/2 signaling in neuronal differentiation of cultured embryonic stem cells Dev Growth ... cannot shorten the time of the neural induction from ES cells The Sox1-GFP+ cells did not appear on differentiation day 2, regardless of the FGF1 treatment The increase of Sox1+ cells in the FGF1-treated...
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báo cáo khoa học: " The HPB-AML-I cell line possesses the properties of mesenchymal stem cells" ppt
... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... Grünwald-Giemsa staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells Unlike the round-polygonal cells, HPB-AML-I cells with the spindle-like ... undifferentiated cells Inverted microscopic examination demonstrated the presence of a number of vacuoles in the cytoplasm of differentiated HPB-AML-I cells (Figure 4G) In contrast to the undifferentiated cells...
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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc
... ratio of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs ... migration of mesenchymal stem cells to conditioned medium from tumor cells or bone marrow cells Stem Cells 2007, 25:520-528 Reya T, Morrison SJ, Clarke MF, et al: Stem cells, cancer, and cancer stem cells ... C: (×400) Area of cell drop out with stem cells, D: (×400) No nodularity & liver appears normal, few collections of round to oval stem cells in lobules Page of 11 Figure Detection of MSCs labeled...
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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps
... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda ... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F,...
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