transcriptome analysis of common gene expression in human mesenchymal stem cells derivedfrom four different origins
Báo cáo y học: " Profiles of cytokine and chemokine gene expression in human pulmonary epithelial cells induced by human and avian influenza viruses" doc
... quantitative differences in the profiles of cytokines/chemokines induced by influenza viruses of different pathogenicity H5N1 was a more potent inducer of inflammatory cytokines/chemokines; particularly ... hours postinfection At hours post-infection, the level of previously elevated cytokines/chemokines still remained at several folds of induction In contrast to the prominent induction of cytokines/chemokines ... 0 10 20 Time post-infection (hr) 30 Figure Kinetics of replication of different subtypes of influenza A virus in NCI-H292 cells NCI-H292 cells were infected with different influenza virus subtypes:...
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báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues" docx
... Comparison of gene expression in different nectary types Comparison of gene expression in different nectary types The number of genes displaying a two-fold or greater difference in expression in different ... (p or q > 0.05) For a more in- depth analysis of genes displaying the largest differences in expression, lists of genes displaying fivefold or greater differences in expression level between MLN ... the first report of a systematic and global interrogation of any nectary transcriptome Not surprisingly, a large number of genes involved in sugar metabolism and processing were differentially...
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Role of sonic hedgehog signalling in human embryonic stem cells and its neural derivatives
... examining the capacity of the SHH pathway in maintaining pluripotent marker expression and cell proliferation of undifferentiated hESC Objective was achieved by studying the effect of overexpression ... culture feeding 3.2.3 Human embryonic stem cells and induced pluripotent stem cells Human embryonic stem cell line HES-3 (46, XX) was from ES Cell International (Singapore, http://www.escellinternational.com) ... lineage Using a defined neural differentiation protocol, it was found that overexpression of SHH in hESC resulted in a significant increase in neural stem cell marker expression as well as increased...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... role in the pathogenesis of intervertebral disc degeneration Arthritis Res Ther 2007, 9:R45 Le Maitre CL, Freemont AJ, Hoyland JA: The role of interleukin-1 in the pathogenesis of human intervertebral ... of outcome measures including use of gene profiling with a custom PCR array of 42 genes associated with a healthy NP phenotype, an important innovation of this study, as well as assessments of ... disc degeneration [43] Minimal changes in COLX expression suggests that NC cells in alginate alone may produce soluble factors capable of limiting or preventing hypertrophy and inhibiting synthesis...
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Báo cáo y học: "Systematic analysis of gene expression in human brains before and after death" docx
... alter gene expression patterns in postmortem human brains Study of expression levels of 14 genes in human brain autopsy and biopsy samples found significant change in one of the genes, indicating ... of genes involved in ubiquitin-dependent protein catabolism may lead to increased degradation of proteins in human brain samples after death This could have consequences for protein studies in ... effect of death on gene expression in human brain, postmortem samples maintain the vast majority of the expression differences that exist between brain regions in vivo However, the amplitude of expression...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... change in gene expression for MMP-9 in normal or degenerate cells or for MMP-13 in degenerate cells (Figure 1a) Interleukin-1-beta The addition of recombinant IL-1β for 48 hours resulted in a significant ... of cytokine stimulation on matrix metalloproteinase (MMP) gene expression in normal or degenerate nucleus pulposus (NP) cells Normal or cells degenerate NP cells were cultured in alginate pellets ... the addition of IL-1β in either normal or degenerate samples Effect of tumour necrosis factor-alpha on expression of interleukin-1 and interleukin-1 receptors The addition of recombinant TNF-α...
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Báo cáo y học: "Social regulation of gene expression in human leukocytes" pps
... green intensity indicating the magnitude of a gene' s relative over -expression in high-lonely indivdiuals, and red intensity denoting the magnitude of relative under -expression A total of 209 ... global gene expression in the human immune system Results Sample characteristics Global gene transcription profiles were assessed in circulating leukocytes from 14 individuals participating in the ... previous findings) [27-30] Thus, reduced expression of GR target genes in leukocytes from high-lonely individuals does not appear to stem from a failure of the HPA axis to maintain normal circulating...
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Analysis of silicatein gene expression and spicule formation in the demosponge amphimedon queenslandica
... comprehensive phylogenetic analysis of this gene family in sponge indicated that silicatein α arose from silicatein β by gene duplication and that silicatein β gene share traits with both cathepsin L and ... silicatein Conservation of gene structure and exon length in silicatein and cathepsin L genes suggests that these genes have preserved an ancestral gene structure common to both gene families in both ... cathepsin L genes family…… 26 Figure 2.6 Sequence motifs of the two main regions defining silicatein genes…………………….28 Figure 2.7 Phylogenetic relationship between silicatein and silicatein like genes...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human galactokinase potx
... group of GHMP kinases in which proton transfer does not play a major role in the rate determining step of catalysis That five of the 10 disease-causing mutations resulted in insoluble protein in ... at some point in the reaction Modest changes in Km,ATP are seen in H44Y and R68C That H44 in uences the binding of both substrates suggests that the binding sites are probably close in space R68 ... isotope effect was measured by determining the kinetic constants as described above in the presence of increasing mole fractions of D2O (Aldrich) Kinetic constants of the mutants The equation for...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... Gavini, N (1999) Genetic analysis of nif regulatory genes by utilizing the yeast two-hybrid system detected formation of a NifL-NifA complex that is implicated in regulated expression of nif genes ... N2-fixing K pneumoniae strain contains only a single NADH oxidizing enzyme, one can expect a different regulation of the nuo-operon in K pneumoniae M5a1 Based on preliminary sequence analysis of ... subsequent selection of single colonies onto plates resulted in loss of the integrated plasmid with an integration frequency of the interrupted nuoCD region in 50% of the integrants fdnG mutant...
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Báo cáo khoa học: Functional analysis of disease-causing mutations in human UDP-galactose 4-epimerase pot
... during purification Kinetic analysis of disease-causing mutations The steady-state kinetic parameters of each of the nine disease-causing mutant proteins were determined (Table 1) In general, little ... compared Disease-causing mutations in human GALE Results Expression and purification of human GALE Human GALE was expressed as an N-terminal hexahistidine fusion protein in E coli and purified ... Disease-causing mutations in human GALE Fig Crosslinking of wild-type and disease-causing mutants of UDP-galactose 4-epimerase (GALE) Crosslinking was carried out with N-(3dimethylaminopropyl)-N¢-ethylcarbodiimide...
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Báo cáo sinh học: "Conservation of core gene expression in vertebrate tissues" pdf
... definitions of conservation The expression of these 1,488 genes in modern-day lineages is shown in Figure Most of these genes have tissue-specific patterns of expression, indicating that the genes ... in fish, on the basis of our SCEs The expression of these 102 genes in the ten common tissues in the representatives of all modern lineages is shown in Figure Because TF binding sites are degenerate, ... species TF genes within the set of unique orthologs were defined by the presence of a DNA-binding domain in the mouse protein sequence in the Pfam database [75] Our list of TF genes is given in Additional...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... Biomarker measurement in arthritis patients originally relied on protein analysis of fluid compartments Blood sampling is often included in clinical trials, and measurement of certain plasma constituents, ... Analysis of individual tissue fragments from within individual synovia demonstrated that using about six tissue samples allows for the detection of twofold differences in gene expression Interestingly, ... estimates of gene expression using image analysis [14] Quantification is improving [15] and radioactive detection systems have a wide linear range, but the linearity of the widely used chemical systems...
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Báo cáo y học: "Comparison of marker gene expression in chondrocytes from patients receiving autologous chondrocyte transplantation versus osteoarthritis patients" pptx
... in type II collagen expression, upregulation of type I collagen expression and a slightly higher expression of ALK-1, showing ongoing dedifferentiation in vitro Since redifferentiation of dedifferentiated, ... stopped by washing with PBS Results Analysis of gene expression patterns in chondrocytes ex vivo In the first set of measurements, gene expression patterns were investigated in cells directly ... these cells show no inclination to become hypertrophic or to retain OA characteristics Gene expression of chondrocytes in scaffolds To investigate the regenerative potential in OA chondrocytes in...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... defects in humans [5,6] However, to date, the delivery of MSCs into cartilaginous lesions has neither clinically nor experimentally resulted in sustained regeneration of hyaline cartilage in vivo ... characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells Arthritis Rheum 2008, 58:1377-1388 36 Miljkovic ND, Cooper GM, Marra KG: Chondrogenesis, bone morphogenetic protein-4 and mesenchymal ... or remained uninfected For comparison, an additional set of untransduced recombinant human protein controls were maintained, which were cultured in the presence of 10 ng/mL TGFβ1 protein, or...
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báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
... be expressed in human embryonic stem cells and other stem cells, indicating that these miRNAs may play a constitutive role in maintaining the biological characteristics of stem cells [40,41] ... regulate genes such as the proto-oncogene bcl-2, thus modulating tumorigenesis [33,34] In this study, miR-21 expression was lower in BCSCs than in MCF-7 cells Interestingly, target analysis of miR-21 ... were downregulated in human BCSCs MiR-200c was shown to be overexpressed in MCF-7 cells, leading to reduced expression of transcription factor and increased expression of E-cadherin [19] Furthermore,...
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báo cáo khoa học: "Transcriptome analysis of ectopic chloroplast development in green curd cauliflower (Brassica oleracea L. var. botrytis)" potx
... showing the number of common genes between HY5-targeted genes in Arabidopsis and total differentially expressed genes in green curds of cauliflower (b) Venn diagrams showing the numbers of common ... changes in expression in green curd mutant Among them, a large number of genes involved in photomorphogenesis including chloroplast development were revealed, demonstrating a successful use of the ... coordinated action of a number of processes, including the biosynthesis of photosynthetic complexes, transportation of nuclear encoded proteins into chloroplasts, processing of the imported proteins,...
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báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps
... p53 induction of mir-21 required activation of NF-κB Next we tested the activity of the putative p53-binding site GIS by cloning it upstream of firefly luciferase and examining reporter gene expression ... control gene expression program in heart failure Understanding the way that different DNA binding proteins interact with DNA regulatory elements and modulate gene expression will provide information ... domain Purified recombinant GST-RELA peptides (amino terminus, DNA binding domain; carboxyl terminus, transactivation domain; in the left panel, the arrow indicates the GST-RELA 428-551 amino...
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Báo cáo y học: "A semi-quantitative GeLC-MS analysis of temporal proteome expression in the emerging nosocomial pathogen Ochrobactrum anthrop" pptx
... MPMNKNELVA 17982154* Leucine, isoleucine, valine, threonine and alanine binding protein MGVPTMRKTLFSGVALAAVIAFGGSAWA 17983192* General L-amino acid-binding periplasmic protein AAPJ precursor MKKTLMTGVLGAAALFGIASGASA ... processes, thus giving insights into the underlying physiology and biochemistry of this organism During the late phase of growth of O.anthropi a number of gene products normally induced in response to ... Rhizobium etli were involved in protein synthesis (ribosomal proteins), followed by those involved in metabolism of nucleotides and nucleic acids, then those involved in metabolism of amino acids and...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... for a single major protein band displaying a molecular weight corresponding to that of the hUCP3 Figure shows the level of hUCP3 expression after infecting L6 cells with increasing amounts of UCP3 ... density of 1.5 · 104 cells cm)2 Infection was carried out 24 h later by incubating the cells for h in the presence of the transfection reagent Lipofectamine Plus combined with adenoviruses containing ... digitonin treatment in order to permeabilize the plasma membrane This was performed by incubating L6 cells at a concentration of · 106 cells mL)1 into NaCl/Pi containing 50 lM digitonin on ice for Cells...
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