... negative phosphates of this state. The most striking effect of mutation of arginine to histidine is analmost 2000-fold decrease in the kcatvalue for dThd,and a 270-fold decrease for dCyd. The Kmvalue ... active(see Table 2).The most striking result for Y70W was that itbecame an almost entirely pyrimidine-specific kinase,because phosphorylation of dAdo and dGuo wasalmost abolished, compared ... levels of phosphorylation expressed in rela-tion to percentage dThd phosphorylation of the wild-type. Relativephosphorylation expressed as a relation of the percentage of dThdphosphorylation of...
... importance of identifying symptomaticsemploying cough of two weeks or more instead three weeks or more for diagnosis of TB. Among the55561 adult outpatients screened, 2210 had cough of two weeks ... additionalcriterion for detection of cases. Two samples of sputum specimens were collected from those whowere symptomatic and/or abnormal on X-raysuggestive of TB and processed for identification of cases. ... Distribution of symptomatics and number of cases identified in three surveysP. G. GOPI ET AL Surveys No. Eligible No. Examined (%) No. of symptomatics (%) No. of sputum examined (%) No. of...
... 17 for more information) 4. Metaphor in poetryThe use of metaphor in poetry is one of the most important aspects of poetic style that must be mastered. Metaphor can be described as figure of ... Before we continue, let us not forget that: “One of the major functions of metaphor is to express emotion” (Goatly 1997: 158).Emotion metaphors form an extensive group in the taxonomy of metaphors. ... realized by means of a clause, but rather by means of another type of form, such as a noun phrase, as in the example at hand. In this sense, grammatical metaphor again involves a type of metaphorical...
... analysis. Investigation of Organic Chemicals Potentially Responsiblefor Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007By David A. ... Laboratory4Friends of the North Fork of the Shenandoah RiverPrepared in cooperation with the Friends of the North Fork of the Shenandoah RiverOpen-File Report 2008–1093U.S. Department of the InteriorU.S. ... 2008, Investiga-tion of organic chemicals potentially responsiblefor mortality and intersex in fish of the North Fork of the Shenandoah River, Virginia, during spring of 2007: U.S. Geological...
... Drug ApplicationORA Office of Regulatory AffairsORO Office of Regional OperationsOSD Office of the Secretary of DefensePB pyridostigmine bromidePEO Program Executive OfficerPHSA Public Health ... distribution unlimitedPrepared for the Of ce of the Secretary of DefenseR Health National Defense Research InstituteHealth The Challenges of Acquisition 15information for the reviewers to evaluate ... thestudy, the Joint Program Office–Biological Defense (JPO-BD) was responsiblefor theacquisition of biological agents. In the final stages of report preparation, that officewas reorganized as...
... were recovered into the organic phase by three- fold extraction with 50% NaCl ⁄ chloroform (1 : 1v ⁄ v). A new volume of chloroform was used for each of the three extractions. Finally, the organic ... developedcountries, more than 80% of children under the age of 3 years will be diagnosed at least once with otitismedia, and M. catarrhalis is responsiblefor 15–25% of all of these cases [4,5]. In adults ... a-galactosyltransferase for addition of the terminal galactose of the LOS [25], and Schwin-gel et al. found the lgt6 gene involved in the initialassembly of the LOS [26]. However, for lipid Abiosynthesis of the...
... communications regulator Ofcom has specified up to age 16 years for controls on marketing specified foods during children’s TV programmes, and the Danish Forum ofResponsibleFood Marketing Communication ... effectiveness of marketing is a function of exposure and power, the overall policy objective should be to reduce both the exposure of children to, and power of, marketing of foods high in saturated ... per Reference Quantity have been determined for each Nutrient to Encourage. 25 TheFoodProfiler is a method of evaluating the nutritional quality of foods according to their potential to ‘rebalance’...
... and expression of cDNA for rat heme oxyge-nase. Proc Natl Acad Sci USA 82, 7865–7869.4 Maines MD, Trakshel GM & Kutty RK (1986) Char-acterization of two constitutive forms of rat livermicrosomal ... Suzuki H, Takeda K,Meguro K & Endo K (1993) Functional analysis of cDNAs for two typesof human heme oxygenase andevidence for their separate regulation. J Biochem(Tokyo) 113, 214–218.10 ... lgặmL)1) for the indicated num-ber of hours. Each lane contains 15 lg of total RNA. The lane labeled 0 h contained RNA prepared from precultured cells harvested justbefore the addition of actinomycin...
... to be informed. Offer special discounts, offers and schemes specifically designed for these shoppers. Although whatever best you do for them, is not enough. Yet the more services you offer them, ... TO HANDLE SUCH TYPE OF CUSTOMERS: These typesof customers can be easily identified by the way they react to the assistance offered to them. Such typesof customers prefer to be left alone and ... argue with these typesof customers and offend them. They are very sure of their decisions and although are ready to experiment with new ideas or suggestions but after a lot of thinking. They...
... Murakami2and Kenji Aoki21Division of Science of Biological Resources, Graduate School of Science and Technology,2Department of Biofunctional Chemistry,Faculty of Agriculture, Kobe University, ... thebiodegradation of nitrobenzenes [1–4]. 2-Aminophenolsserve not only as a carbon source, but also as a nitrogensource for g rowth of the assimilating bacteria. Deaminases,which catalyze the release of ... peak of the purified enzyme at 266 nm. The typicalprotein absorption p eak of 2 80 nm shifts to 265 nm if theprotein contains a flavin-type cofac tor [28]. We failed toidentify the cofactor of...
... thatimproves κ is a combination of Prominent Relation-ship with Argument Types (combined κ of 0.250, ascompared with 0.145 for Prominent Relationship and0.180 for Argument Types) . However, we believethis ... x is of type ‘person’.” Statedin this way, these kinds of knowledge look quite dif-ferent. If one reads our proofs from start to finish,the flow of the argument indicates which of theseforms ... ourset of categories. Further surveys would be requiredto validate this idea.The 20 categories of knowledge covered 215(97%) of the 221 statements of world knowledgein our proofs. Of the...
... alleles may be responsiblefor the generation of four possible allelic lineages (represented by A, B2, B1,and W ), one of which (W/M) i s r evealed by this work. Thehigh polymorp hism of the goat ... previouslymentioned, we were working with a mixture of twounresolvable variants, one of which (M) a ccounts for morethan 80% of the whole as1-Cas. This overrepresentation of the M variant allowed us to continue ... a t the as1-Cas locus. There-fore, analysis o f their transcripts could have been ratherdifficult because of the occurrence of at least nine differentforms of messenger arising from the F...
... determinant responsiblefor PKAphosphorylation in vitroTo identify the structural determinants that are respon-sible for phosphorylation of GIRK1 by PKA, fusionproteins comprising truncated forms of ... distalpart is unique for GIRK1 among the Kir3.x isoforms.Further truncations of the C-terminus revealed a 49amino acid stretch (amino acids 362–411) that was the most prominent target for phosphorylation ... amount of incorporated radioactive32P. The most effective result was obtained by simulta-neous mutation of all three S ⁄ Ts (subsequently denoted3*), resulting in an almost complete absence of...
... abolishes proper nucleartargeting of the mSMOl isoform.ResultsStructural analysis and modeling of vertebrateSMO proteinsComparison of the derived amino acid sequences of vertebrate SMO proteins ... mammalian-specific function of SMO (e.g. nucleartargeting of the mSMOl isoform). Inspection of themSMOl modelled structure (Fig. 2) indicates that bothregions are located on the tip of the FAD-bindingdomain, ... between the two isoforms is the presence of an extra protein domain in mSMO l, encoded by theexon VIa [10].Comparative analysis of the amino acid sequence of the vertebrate members of the SMO family...
... positive for catalase, oxidase andnitrate reduction but negative of hydrolysis for gelatin andstarch. Therefore, according to all above analysis results, thestrain Iso1 was clearly a strain of V. ... Optimal temperature and thermostability of N-D-AAase. (A)The optimal temperature of purified enzyme. Enzyme activity mea-surements were performed at various temperatures for 20 min. Thehighest ... Downstream of the stop codon, aterminator was found and the pI was predicted to be 5.80 bythe use of the N-D-AAase amino acid composition in theDNASISsoftware package.Sequence comparison of theV....