thermodynamics protein modification and molecular dynamics in characterizing lactose repressor protein strategies for co
Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt
... in conformational adaptability of bovine myelin basic protein Proteins 7, 32–40 Ramwani, J.J., Epand, R.M & Moscarello, M.A (1989) Secondary structure of charge isomers of myelin basic protein ... epitope in the N-terminus could increase the binding and affinity for I-Au because of occupation of the P1 pocket Recent thermodynamic and kinetic studies of the binding of TCRs to peptide–MHC ligands ... S.W (1984) Proteins of myelin In Myelin, 2nd edn (Morell, P., ed.), pp 197–224 Plenum Press, New York Smith, R (1992) The basic protein of CNS myelin: its structure and ligand binding J Neurochem...
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Báo cáo khoa học: Structural flexibility in Trypanosoma brucei enolase revealed by X-ray crystallography and molecular dynamics pdf
... chemistry involving a Lys-Glu dyad [8,9] Also essential is the binding of two divalent metal ions to distinct sites: the first ‘conformational’ site being required for substrate binding and the second ... sulfate-bound form, including one containing a novel metal binding site Furthermore, they show structural heterogeneity in their inhibitor-bound forms, highlighting the potential to extend future inhibitors ... and Glu208, His156 is the only protein ligand of the metal in site IV (Fig 5) Zinc ions bound by single protein ligands are comparatively rare in the Metalloprotein Database [33] but there are...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1
... the Transforming Acidic Coiled Coil protein, Mia1p, functions in sustaining proper MTOC and microtubule dynamics, both in interphase and mitosis Briefly, I show that Mia1p is required for microtubule ... Liling Zheng , Cindi Schwartz , Liangmeng Wee , and Snezhana Oliferenko (2006) The Fission Yeast Transforming Acidic Coiled Coil–related Protein Mia1p/Alp7p Is Required for Formation and Maintenance ... essential to understand molecular mechanisms underlying MTOC emergence and maintenance In this thesis, I use a combination of cell imaging, cell manipulation and genetics experiments in the fission...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 2
... by depositing the kelch-repeat protein, Tea1p, at the cell tips There, Tea1p functions in polarity establishment by recruiting a complex, forminbinding protein Bud6p and the formin, For3 p, to ... bundles during interphase My work during my Ph.D project has concentrated on uncovering the molecular functions of Mia1p and understanding how it might be involved in supporting microtubule dynamics ... D-TACC protein, another centrosomal protein -Minispindles (Msps), making a breakthrough in understanding normal function of TACC protein (Lee et al., 2001) Msps belongs to the highly conserved...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 3
... and resuspended in 40μl TE buffer containing RNAse and incubated at 37oC for 20min DNA was stored at -20oC 2.3 Construction of knock out mutants and epitope tagging of genes 2.3.1 Construction of ... Imaging, Sunnyvale, CA) For imaging of microtubule dynamics, S pombe cells expressing αtubulin-GFP were grown in appropriate selective medium and placed in sealed growth chambers containing 2% ... filter and incubated in the PEMG buffer [100mM piperazine-N, N’-bis(2-ethanesulfonic acid), PH6.9, 2mM EGTA, 1mM MgSO4 and 1mM GTP] containing 5% milk and then with taxol-stabilized bovine brain...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 4
... coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p-overexpressing cells are bipolar and require the microtubule-crosslinking protein, Ase1p, for ... spindle poles and NE division fails in Mia1poverexpressing cells Since the SPBs are thought to be required for mitotic spindle assembly and function in fungi, and are always localized at spindle ... B) Since Mia1p has been found to be involved in maintaining stable microtubule bundles during interphase, I decided to check whether any aspects of the eMTOC/PAA dynamics could be affected in...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 5
... TACC proteins contain extensive coiled coil regions and can likely form oligomers This, in combination with their microtubule binding properties, could provide a framework for clustering and focusing ... and antiparallel spindles (see Figure 3.2.3B&F) These spindles exhibited a well defined spindle midzone and contained the γ-TuRC complexes and the BimC kinesin, Cut7p, at 112 acentrosomal spindle ... indicating that in addition to Mia1p, other protein( s) is/are function in parallel pathway to anchor microtubule to the SPB during interphase One possible candidate involving in anchoring minus...
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Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 6
... M., Vince, P., Wolfman, A., and Cowell, J.K (1999a) Cloning of TACC1, an embryonically expressed, potentially transforming coiled coil containing gene, from the 8p11 breast cancer amplicon Oncogene ... Still, I.H., Vince, P., and Cowell, J.K (1999b) The third member of the transforming acidic coiled coil-containing gene family, TACC3, maps in 4p16, close to translocation breakpoints in multiple ... N., and Toda, T (2004) Interdependency of fission yeast Alp14/TOG and coiled coil protein Alp7 in microtubule localization and bipolar spindle formation Mol Biol Cell 15, 16091622 127 70 Sawin,...
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A modeling study of ion implantation in crystalline silicon involving monte carlo and molecular dynamics methods
... Scientific (U.K), Integrated Systems Engineering (ISE, Zurich), Institute of Materials Research and Engineering (IMRE, Singapore), Institute of High Performance Computing (IHPC, Singapore) and the department ... Phaseshift for the scattering of an electron EF Fermi energy xx CHAPTER 1.1 INTRODUCTION Motivation The main driving force for performance in the semiconductor industry lies in the need to increase ... profiles in silicon (Hofker et al., 1975), Ryssel et al (1980) found that this is also true for boron in SiO2 and Si3N4, for arsenic in silicon, SiO and Si3N4, and all other combinations that were investigated...
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Inorganic molecular sieves Preparation, modification and industrial application in catalytic processes
... nm specifications according to the new and stringent legislative requirements In addition, during hydrocracking operation sulfur and nitrogen-containing compounds are removed and saturation of ... but, in the last years, there has been an increasing trend in using multi-component catalysts, where the acidic zeolite is combined with amorphous silica alumina (ASA) and an alumina binder In ... petrochemical industry [27] Applications for the chemical industry involve mainly oximation, epoxidation, acylations, condensation and amination processes Moreover, their presence is increasing in emerging...
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Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf
... KCl containing varying concentrations of UDP-Glc (0–145 mM) were studied The variable concentrations of UDP-Glc were obtained by starting with a stock solution containing all of the components including ... metal binding event would be totally independent of the interactions involved in the enzyme–substrate complex The binding of MUG and Mg2+ are not independent, however, but instead are highly coupled ... fluorescence were performed at 50 nM MUG in 50 mM Hepes, pH 7.6 and 150 mM KCl Binding titrations were performed in solutions containing 50 nM MUG in 50 mM Hepes, pH 7.6, 150 mM KCl, and mM dithiothreitol...
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Clinical and Molecular Advances in Ankylosing Spondylitis Edited by Jacome Bruges-Armas doc
... another being the sternoclavicular joint The disc divides each joint into two The lower joint compartment formed by the mandible and the articular disc is involved in rotational 16 Clinical and Molecular ... from first symptoms onset Glucocorticoids Constant DAR2 BMI DAR1 Glucocorticoids IM2 Constant DAR2 BMI IM2 Glucocorticoids DAR1 Constant Regression coefficient (B) (standard error) -.124 (.027) ... and jaw and is worsened with chewing Headache and/ or neck ache: In some cases, patients may complain of headache without localized pain in the temporomandibular joint A bite that feels uncomfortable...
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High-Tech Start-Ups and Industry Dynamics in Silicon Valley potx
... and their linkage to Asia, the role of U.S tariff policy and its effect on increasing export activity, and the role of exports and foreign direct investment in building California’s economy for ... examining Silicon Valley’s high-tech economy in a dynamic context Using two unique longitudinal databases, we investigate firm formation, growth, mortality, and migration in Silicon Valley during ... except for “moving inside” Silicon Valley, which is not a major concern of our study Death Merger and acquisition Moving inside Moving out Moving in Growth Birth Figure 1.2—Industry Dynamics in Silicon...
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democratic drift majoritarian modification and democratic anomie in the united kingdom jan 2010
... distrust and disengagement remain high This was the driving force behind the Independent Inquiry into Britain’s Democracy (The Power Report) in 2006 and which led Colin Hay, in his in uential ... Oxford University’s Centre for Socio-Legal Studies for coordinating a project on Charter 88 and democratic change; Steven Fielding of Nottingham University for convening a conference on the Conservative ... trust and increase in disenchantment that occurred during the final years of New Labour This book examines Brown’s constitutional inheritance and provides a framework for mapping and considering...
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CLINICAL AND MOLECULAR ADVANCES IN ANKYLOSING SPONDYLITIS pptx
... another being the sternoclavicular joint The disc divides each joint into two The lower joint compartment formed by the mandible and the articular disc is involved in rotational 16 Clinical and Molecular ... from first symptoms onset Glucocorticoids Constant DAR2 BMI DAR1 Glucocorticoids IM2 Constant DAR2 BMI IM2 Glucocorticoids DAR1 Constant Regression coefficient (B) (standard error) -.124 (.027) ... and jaw and is worsened with chewing Headache and/ or neck ache: In some cases, patients may complain of headache without localized pain in the temporomandibular joint A bite that feels uncomfortable...
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Báo cáo khoa học: "Effects of sylvicultural practices on nutrient status in a Pinus radiata plantation: Nutrient export by tree removal and nutrient dynamics in decomposing logging residues" ppt
... logging residues were incorporated into the Figure Remaining slash needles (a) and twigs (b) after decomposition in the thinned stand and in the prepared and unprepared plots after clear-felling ... a thinned stand and in an adjacent clear-cut area In the latter, the effect of intensive site preparation involving logging residue incorporation to the mineral soil was compared with the conventional ... Litterfall in the thinned stand was collected monthly in each of six litter traps (0.25 m2) located at random in the plot, and analysed Six rain gauges were set at random in the plot and in the nearby...
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Báo cáo khoa học: "Organic matter and nitrogen dynamics in a mature forest of common beech in the Sierra de la Demanda, Spain" doc
... the N contents in green leaves and the contents of this element in shed leaves) As is logical, the highest IR index was found at the beginning of autumn and the lowest in spring Accordingly, ... production and N and P cycling along a nitrogen mineralization gradient on Blackhawk Island, Wisconsin, Ecology 65 (1984) 256–258 [65] Prescott C.C., Corbin J.P., Parkinson D., Biomass productivity and ... Ecol (1987) 293–296 [8] Berg B., Dynamics of nitrogen (15N) in decomposing Scots pine (Pinus sylvestris) needle litter Long-term decomposition in a Scots pine forest VI., Can J Bot 66 (1988) 1539–1546...
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Biochemical, Genetic, and Molecular Interactions in Development - part 1 docx
... concentration (21) In tandem, BMPs are modulated by BMP-binding proteins and other growth and differentiation factors, resulting in combinatorial signaling and divergent outcomes depending on genetic ... mature protein consists of five tandem repeat domains termed cadherin repeats, each of which consists of approx 110 amino acids The cadherin repeats form four Ca2+-binding domains, and the N-terminal ... or in conjunction with -actinin Other proteins also bind the cytoplasmic domain of N-cadherin, such as p120ctn and the nonreceptor protein tyrosine phosphatase 1B (PTP1B) Both of these proteins...
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Biochemical, Genetic, and Molecular Interactions in Development - part 2 potx
... morphogenetic proteins differentially modulate progressive CNS lineage fate Dev Neurosci 22, 74–85 22 Reddi, A H (2001) Interplay between bone morphogenetic proteins and cognate binding proteins in bone and ... deleted gene for type I procollagen (COL1A1) A breeding line with a phenotype of spontaneous fractures and decreased bone collagen and mineral J Clin Invest 91, 709–716 171 Forlino, A., Porter, ... Development In the absence of a transgenic mouse expressing, in a inducible manner, cre recombinase in proliferating and prehypertrophic chondrocytes, we hypothesized that maintaining Cbfa1 expression in...
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Biochemical, Genetic, and Molecular Interactions in Development - part 3 pdf
... 1(XXVII) COL4A1 COL4A2 COL4A3 COL4A4 COL4A5 COL4A6 COL5A1 COL5A2 COL5A3 COL5A4 COL6A1 COL6A2 COL6A3 COL7A1 COL8A1 COL8A2 COL9A1 COL9A2 COL9A3 COL10A1 COL11A1 COL11A2 COL2A1 COL12A1 COL13A1 COL14A1 COL15A1 ... b Gene COL1A1; COL1A2 COL2A1 COL3A1 COL4A3; COL4A4; COL4A5 COL4A5 and COL4A6 COL5A1; COL5A2 COL6A1; COL6A2; COL6A3 COL7A1 COL8A2 COL9A1; COL9A2; COL9A3 COL10A1 COL11A1; COL11A2 COL17A1 COL18A1 ... Tissues containing type I collagen Nervous system Most connective tissues Anchoring fibrils in skin, cornea, cervix, oral, and esophageal mucosa Many tissues Tissues containing type II collagen...
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