the qst fst comparison applied to two different ecotypes of the lake whitefish coregonus clupeaformis
Population Genetics for Animal Conservation potx
... merely that of inferring the values of the Wi variables On the other hand, estimating the proportion of fish from different populations is just the process of inferring the value of π Finally, ... that denotes the population of origin of the ith fish It can be thought of as a ball that is tied to the fin of the fish, with the colour of the ball telling us where the fish comes from Under ... preparation of this book and the organization of the conference on Population Genetics for Animal Conservation from the Ministry of Research and Innovation of the Autonomous Province of Trento, the Edmund...
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Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
... particularly the striatum, can oxidize retinol to retinal, providing the first step on the way to retinoic acid [31] The brain must thus rely on the activity of other enzymes, for example ADH3 or other ... findings in adult brain tissue of all three species Discussion The distribution of ADHs in the brain is of particular interest because of their implications in the metabolism of ethanol and retinoic ... the promoter [12] In these mice, we could clearly demonstrate absence of Adh4 mRNA in the stomach epithelia – the tissue with the best characterized Adh4 expression Taken together, our results demonstrate...
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Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx
... excluded The observed expression of the TPH gene in spleen and pituitary deserves further study to assess the possible production of serotonin by these organs Our extensive molecular analyses of AANAT ... to the oxidation of melatonin [47,48] To summarize, mouse skin has both the capability of producing serotonin and the machinery for its extensive metabolism Data currently available suggest the ... products of NAS metabolism (for details see Discussion) to NAS would limit serotonin effects in the skin (proedema, vasodilatory, pruritogenic and proinflammatory activities) The further metabolism of...
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báo cáo hóa học: " Reduced inflammation accompanies diminished myelin damage and repair in the NG2 null mouse spinal cord" potx
... multiplied by 10 to obtain the demyelinated volume for that particular segment of 10 sections, and all 20 values were then summed to obtain the total volume of demyelination For animals of the same genotype ... according to guidelines issued by the National Institutes of Health, following procedures approved by the Office of Laboratory Animal Welfare All experimental protocols were approved by the Sanford-Burnham ... attributed to several factors, including failure of OPC proliferation, failure of OPC recruitment to the lesion, failure of OPC differentiation, and failure of OPCs or oligodendrocytes to interact with...
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báo cáo hóa học:" Progressive obesity leads to altered ovarian gene expression in the Lethal Yellow mouse: a microarray study" pptx
... control confirming the comparability of the two sets of microarrays Conversely, the Ay mutation leads to a reduced expression of the Raly gene which was expressed at half the level of black mice in ... CodeLink software (Applied Microarrays, Tempe, AZ) applied the background correction GeneSpring 7.0 software (Agilent, Santa Clara, CA) was used to normalize the expression of each gene to the median ... in the final data set After these exclusions, 52 of the roughly 3.3 × 104 genes analyzed with the microarrays showed statistically significant differential expression Twenty-eight of the differentially...
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Báo cáo y học: "Hepatocyte growth factor ameliorates dermal sclerosis in the tight-skin mouse model of scleroderma" doc
... accordance with the guidelines of the National Institutes of Health (Bethesda, MD, USA), as specified by the animal care policy of Hyogo College of Medicine Expression vector and preparation of liposomes ... analysis of cDNA for human hepatocyte growth factor Biochem Biophys Res Commun 1989, 163:967-973 Zarnegar R, Michalopoulos GK: The many faces of hepatocyte growth factor: from hepatopoiesis to hematopoiesis ... participated in the design and co-ordination of the study, and participated in the interpretation of the results T Imado and SK performed the animal study and histologic analysis HS participated in the design...
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Báo cáo y học: "Analysis of C4 and the C4 binding protein in the MRL/lpr mouse" pptx
... preventing the formation of the C3 convertase by binding to C4b; accelerating the natural decay of the classical pathway C3 convertase; and as a cofactor for the serine proteinase factor I in the proteolytic ... injury in the MRL mouse is independent of Fc receptors [51], our studies showing the functional deficiency of C4 in these mice, and that the loss of the major negative regulator of the classical ... believed to be due to the inefficiency of C3b binding to the immune complex: only 10% of generated C3b binds to the Available online http://arthritis-research.com/content/9/5/R114 complex Loss of the...
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báo cáo khoa học: " Increased hepatic oxidative metabolism distinguishes the action of Peroxisome proliferator-activated receptor d from Peroxisome proliferatoractivated receptor g in the ob/ob mouse" ppt
... β-oxidation of fatty acids in the liver, the production of acetyl-CoA can exceed the capacity of the TCA cycle The excess acetyl-CoA is converted to β-hydroxybutyrate through ketogenesis in liver mitochondria ... and Pareto-scaled prior to analysis Pareto scaling involves weighting each of the variables by the square root of that variable’s variance, minimizing the impact of noise and increasing the importance ... Agonist To examine the fate of the increased serum β-hydroxybutyrate produced by the liver through increased fatty acid oxidation following exposure to the PPARδ agonist, the metabolome of skeletal...
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Báo cáo y học: "Quantifying the major mechanisms of recent gene duplications in the human and mouse genomes: a novel strategy to estimate gene duplication rates" docx
... retroposition The rates are the slopes of the linear functions (red lines) fitted to the curves of the cumulative distributions of retrogenes All of the linear functions are fitted to the part of the curves ... into two parts using Ks = 0.25 as the cut-off and fitted linear models to each part of the curves The results are shown in Table The slopes of the linear functions are therefore the estimates of ... method to obtain the data, they claimed that the lower rates are due to the removal of the effect of gene conversion on the data However, our results show that most of the statistics in two- copy...
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Báo cáo y học: " Gene expression regulation in the context of mouse interspecific mosaic genomes" pdf
... obliges trans-regulators of one species to act on cis-elements of another species In the hybrid, by contrast, the co-existence of two 'hemigenomes' allows trans-factors to find their bona fide targets ... grouping of genes according either to a given function or to a specific keyword This is likely due to the presence of more than one M spretustype transcription factor in the segments, each of them ... summary of the modes of regulation encountered in the IRCSs and their parent strains A summary of the modes of regulation encountered in the IRCSs and their parent strains Blue marks the B6 chromosome...
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Role of SPHK2S1P signalling in regulating mitochondrial function in the MPTP induced mouse model of parkinsons disease and in the MPP treated MN9D cells
... one of a simply sporadic premise to the view that both environmental and genetic factors help the onset of the disease 1.7.1 Aging- The cardinal factor Age is one of the prominent risk factors ... in the development of PD (Gorell et al., 1998) Nevertheless, the results were not consistent The major drive for the environmental factors to be the major cause of PD came from the discovery of ... on the ability of S1P to activate p-CREB, our results showed that S1P can activate P-CREB in the MPP+-treated MN9D cells The localization of Sphk2 in the mitochondria and the ability of S1P to...
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Tài liệu Báo cáo khoa học: Treatment of neutral glycosphingolipid lysosomal storage diseases via inhibition of the ABC drug transporter, MDR1 Cyclosporin A can lower serum and liver globotriaosyl ceramide levels in the Fabry mouse model doc
... disease, the primary pathology is in the kidney [1], the major site of Gb3 synthesis in man [11], and in the heart, possibly due to the association of Gb3 synthesis with the microvasculature GSL synthesis ... considered that the increased renal Gb3 of the Fabry mouse might extend to the glomerulus to provide a model of the human disease In the cortex of wild-type kidney, subpopulations of renal tubules ... lL of plasma and then partitioned against ⁄ volume of water The lower phase was dried under a stream of nitrogen gas and then the residue was dissolved in chloroform The sample was applied to...
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Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... upstream of the major transcription start site The presence of a CCAAT box and the absence of a TATA box seem to be one of the characteristics of the mouse integrin a3 gene We identified two transcription ... However, it remains to be identified which transcription factor of the Ets-family is involved in the regulation The members of the Ets-family of transcription factors bind to specific purine-rich ... and the sets of primers listed in Table After the PCR products were digested with KpnI and SacI, the fragments were inserted into the KpnI/SacI site of Fig Structures of the 5¢-flanking region of...
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Báo cáo khoa học: Investigating the role of the invariant carboxylate residues E552 and E1197 in the catalytic activity of Abcb1a (mouse Mdr3) docx
... relating to the mechanism of action of ABC transporters remain obscure An important issue is the catalytic mechanism of ATP hydrolysis by the two NBDs, which can be further subdivided into two major ... reproducibility of our current assay; and would represent < 1% of the activity of the wild-type enzyme We then determined, by photoaffinity labeling, whether any of the mutations altered the apparent ... for the IC residues is starting to emerge; first the ICs appear to be important in NBD– NBD communication and transmission of the nucleotide state of one active site to the other; second, the...
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Báo cáo khoa học: Structural and functional consequences of single amino acid substitutions in the pyrimidine base binding pocket of Escherichia coli CMP kinase pdf
... CMP The side chain of R110 is a hydrogen bond donor to the N3 atom of cytosine As the main chain carbonyl of D129, a H-bond acceptor, interacts with the terminal oxygen of S36 side chain, the ... energies of the original resiDG i;j due i in the native protein with the rest of the residues, self DDG k mutị are the changes of the self energies of the residues k that change either their ionization ... or R110 disrupts the salt bridge D132R110 and causes the partner side chain to adopt a different conformation, thereby reducing the effect of the mutation The other two types of relaxation are...
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Báo cáo khoa học: Secondary structure assignment of mouse SOCS3 by NMR defines the domain boundaries and identifies an unstructured insertion in the SH2 domain pdf
... injection of a total of 290 lL of 80–200 lm of the gp130 peptide The heat of dilution of the gp130 peptide into buffer was determined in control experiments and subtracted from the raw data of the ... surprising in view of the hypothesis for its mechanism proposed by Yasukawa et al [6] This requires the KIR to structurally mimic the activation loop of JAK2, so that in the absence of JAK2 the KIR would ... because of the higher solubility of the former SOCS1, based on the similarity of the ESS sequence to a similar region in Stat1 [8] and Stat3b [9], and suggested that the ESS and KIR form two short...
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Báo cáo khoa học: New members of the brachyurins family in lobster include a trypsin-like enzyme with amino acid substitutions in the substrate-binding pocket potx
... comparative modelling The aim of the present work was to study the diversity of trypsins in the digestive gland of P argus, with focus on: (a) the position of lobster trypsins within the brachyurins ... structure of the chymotrypsin fold consisting of two six-stranded b-barrel domains packed against each other, with the catalytic residues (His74, Asp125, Ser218) located at the junction of the two ... the rest of P argus enzymes and other crustacean strict trypsins, this enzyme could not be allocated to any of the brachyurins types due to amino acid substitutions found in the vicinity of the...
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Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deficient mouse embryonic fibroblast cells pdf
... to nearly half the initial amount On the other hand, the amount of glycoprotein in the medium did not differ substantially from the beginning to the end of the experiment, because the medium was ... other sources We then determined the variation of amount of glycoprotein in cells As shown in Fig 5E, although the total amount of protein in cells decreased to 87% after 48 h of incubation, the ... according to the manufacturer’s instructions Briefly, the mutant bovine DNase I cDNAs were subcloned into the pENTER 1A vector After purification of the plasmids, the cDNA inserts were transferred to the...
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Báo cáo khoa học: Identification of an antibacterial protein as L-amino acid oxidase in the skin mucus of rockfish Sebastes schlegeli pot
... the antibacterial activity of SSAP was examined The addition of catalase almost completely abolished the antibacterial activity of SSAP, indicating that H2O2 is the mediator of the activity of ... consistent with those of the FAD standard Figure 5B shows the mass spectrum of the chromophore from SSAP ESI-TOF-MS in the positive mode gave the main peak at m ⁄ z 786.32 and the ion peaks at m ... consisting of 75-kDa subunits by SDS ⁄ PAGE, partly because of the scarcity of the protein of interest In this study therefore we isolated the antibacterial protein, SSAP, from the skin mucus of S...
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Báo cáo khoa học: Dual expression of mouse and rat VRL-1 in the dorsal root ganglion derived cell line F-11 and biochemical analysis of VRL-1 after heterologous expression pptx
... ligated into lL pcDNA3.1/CT-GFP-TOPO vector in the presence of lL salt solution for at RT The accuracy of the entire open reading frame was checked by DNA sequencing of both strands To obtain the full ... cloning the open reading frame of rVRL-1 from rat brain RNA without the last codon for amino acid P761 and the stop codon into pcDNA3.1/CT-GFP-TOPO vector The resulting sequence was identical to GenBank ... for authentic DRG cells They showed that several features of differentiated DRG cells are present in F-11 cells, e.g release of substance P, the presence of l- and d-opioid receptors, receptors...
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