... measure ofthe uptake capacity, and K0.5 as well as the ratio between Vmax and K 0.5 are measures ofthe affinity ofthe uptake system Also the slope, a, ofthe initial linear part ofthe uptake ... K0.5 as estimated by the modified Michaelis-menten model (Vmax/K0.5) and the slope ofthe initial part ofthe uptake curve (a) as a measure ofthe affinity for P uptake of Cladium mariscus spp ... the effects of oxygen differed between the species (figure 3c) Affinity for P-uptake The slope ofthe initial linear part ofthe uptake curve at low P solution concentrations, a, as well as the...
... consequence of decreased catalase activity in the lung during the development of fibrosis, we used acatalasemic mice (C3H/AnLCsbCsb) The untreated lungs of acatalasemic mice possess only 8.1% of catalase ... found in the present study that the lung tissues of acatalasemic mice possess only 8.1% ofthe catalase activity of wild-type mice, although acatalasemic mice demonstrate equivalent levels of catalase ... Bronchiolar epithelium is a major site of catalase expression in normal adult lungs Decrease of catalase in bronchiolar epithelium and in abnormal re-epithelialization suggests the presence of intracellular...
... into the regional effects of catecholamines Nevertheless, for the safe and beneficial use of catecholamines in the stabilisation of septic patients we need hard data concerning the effects of patient ... [13] What is the clinical relevance of an increase in blood flow in a hepatic vein? What is the relevance of a changed laser Doppler signal from the gastric mucosa, a technique used in the recent ... loading Such effects could explain the several studies investigating the effects of catecholamines using the same techniques but producing controversial results [6,7] There is no doubt that well-performed...
... downstream ofthe stop codon The mammalian expression vectors containing each ofthe two catalase derivatives (catalase and catalase-SKL) were constructed essentially as the (His)6-tagged human catalase ... consumed by the peroxisome results in the formation of hydrogen peroxide Decomposition of this potentially toxic metabolite by catalase is therefore another fundamentally critical function ofthe organelle ... et al., 1998) The ability to balance the generation and decomposition of ROS prevents oxidative damage to the peroxisome and other cellular components ofthe cell Disruption ofthe ability to...
... space velocity of 1.2h-1 The dashed curve shows the n-butane conversion for the Ni and the solid curve is for the Au/Ni supported catalyst 20 Figure 3.1 Typical flow-chart of VASP for the self consistent ... conditions The presence of subsurface carbon significantly decreases the activity of Ni catalysts and the methane activation energy increases from 101 kJ/mol to 143 kJ/mol, when all the sites in the ... design Ni catalysts with improved stability To develop a molecular level understanding ofthe coking mechanism on Ni catalysts, the stability of different forms of carbon that can exist on Ni catalyst...
... and the origin of catalyst promotion, as well as to the design of a new, improved catalyst The starting point to the design of an ammonia catalyst is the volcano-shaped relation between the ammonia ... the anti-symmetry ofthe wave function required by the Pauli principle Correlation is a consequence ofthe charge ofthe electrons on the pair density Further, the second Hohenberg and Kohn theorem ... Ni catalysts This thesis is organized as follows In chapter 2, an overview is given ofthe state-ofthe-art in first principles based design of metal catalysts In chapter 3, the theory and the...
... ofthe world and make them my disciples; baptize them in the name ofthe Father, the Son and the Holy Spirit” (Matt 28: 19) The first approach to different peoples came at Whitsuntide, when the ... administration ofthe sacraments, or other parts ofthe liturgy, frequently may be of great advantage to the people, the limits of its employment may be extended This will apply in the first place to the ... of secular priests (no monks, no orders) For the dispatch of these three Vicars, the words ofthe Magna Carta di Propaganda were cited as an indication ofthe right way to proceed – now for the...
... The correct NVP setting is necessary; it has an influence on the determination ofthe cable length by the instrument Notes on the use of hand-held test devices for installation ofthe KRONE Cat. 6 ... The correct NVP setting is necessary; it has an influence on the determination ofthe cable length by the instrument Notes on the use of hand-held test devices for installation ofthe KRONE Cat. 6 ... The correct NVP setting is necessary; it has an influence on the determination ofthe cable length by the instrument Notes on the use of hand-held test devices for installation ofthe KRONE Cat. 6...
... ruling country with the Communist Party of Vietnam (CPV) as the leading force ofthe government Most ofthe public officials are also members ofthe party and the notion of “civil servant neutrality” ... training of civil servant The definition of training was provided in the order (ukaz) No 983 ofthe President ofthe Russian Federation of September 1997 on “Complete methods for the training of State ... system to methods of merit selections; so that the most competent politically acceptable person gets the job In the longer term, the further strengthening ofthe principle of merit in the Vietnamese...
... Although the structures ofthe mature native form of cathepsin B clearly exposed the relevance ofthe occluding loop for the exopeptidase activity [11], they not explain the mechanisms of endopeptidase ... site, the farther away the occluding loop residues are shifted As seen in the tetragonal form ofthe cathepsin B chagasin complex (3CBK), the depth ofthe binding of inhibitor as well as the shift ... 1) between stefin A and the different enzymes is the average distance between the centre of mass of CA atoms ofthe stefin molecule and the centre of mass ofthe CA atoms ofthe reactive site cysteine...
... mutant of PfTIM need to be rationalized: (a) the reduced stability ofthe dimeric structure, and (b) the signicantly lower value of kcat, suggesting an impairment ofthe catalytic efciency (kcat ... and in the presence of denaturants [27] Extending these studies, we examine here the effect of increasing the bulk ofthe residue at position 74 Surprisingly, the Y74W mutant exhibited loss of both ... reduction in kcat compared to the WT* enzyme There are two possible reasons for the low activity ofthe Y74W* mutant: (a) introduction ofthe bulkier residue at the interface in place of a tyrosine...
... the final evaluation ofthe kinetic parameters in the presence of stearate Evaluation of kinetic parameters Fig Comparison of three models for the catalytic action of plasmin in the presence of ... decrease of plasmin activity in the course ofthe assay in the presence of stearate (Table 1) The good fit of Model III progress curves to the experimental data supports the concept that in the presence ... to the presence of fatty acids (Fig 8B) These results preclude the catalytic domain as a target ofthe Table Catalytic efficiency [the catalytic constant ⁄ Michaelis constant (kp ⁄ Km) ratio] of...
... [11,12] The study of these events is taxing, as the biosynthesis of glycoproteins is not template driven This results in the formation of so-called ‘glycoforms’ [11,12], proteins with the same ... withstanding these clear demonstrations ofthe utility of linear ligation assembly, a convergent chemoselective approach can offer the key advantages of more ready and flexible modification of a well-defined ... incorporation ofthe polar domain [47] In an example that combines the exploration of two modes of modification, ‘polar patch’-modified enzymes have also been applied to the catalysis of glycan-modified...
... electrostatic potentials (EP) for the Ne ofthe lysine residues of a-CT at pH EP, EwaldEi EP is the Ewald energy of placing a charge of +1 at the location ofthe ith ionizable atom [89] Reactivity ... some of these phenomena may be interconnected within the catalytic mechanism ofthe enzyme Structural insights into the mechanochemical nature of a-CT catalysis A more detailed analysis ofthe ... methodology to the study ofthe kinetics of catalysis by the serine protease a-CT we were able to statistically correlate the structurally dynamic behavior ofthe enzyme with its kinetics of catalysis...
... outside the line ofthe aisles as at Wells, the total width ofthe West front, 145 feet, being nearly the same in both cases There are still indications ofthe position ofthe great west door, but the ... the east ofthe cathedral was the Bishop's Palace, the gardens of it extending over the detached burial ground of St Michael's to the east of Priory Street The grandeur of this assemblage of buildings ... is also the logical outcome ofthe space afforded by the level lines of a wooden roof just as the use ofthe pointed window follows from the use of pointed vaulting The treatment ofthe angles...
... and 235 [9] Of theses residues, only Arg213 is in the vicinity ofthe active site of RTA In order to establish the role of arginyl residues around the active site of RTA in the binding of rRNA, ... and, as such, these mutants could not be purified To assess the effect of substitutions made at each ofthe arginyl residues on catalytic activity of RTA, the ability of each ofthe purified RTA ... from the Ca atoms ofthe wild˚ type crystal structure [11] of 0.33 A The electron density in the area local to the substitution is shown in Fig The positions of catalytic residues and all other...
... in the case of equal lengths, alphabetically The division is based on the evolutionary trees (Fig 4) For the amylopullulanase from Thermococcus hydrothermalis (Q9Y8I8_THEHY), the numbering ofthe ... data) Therefore, in attempt to provide the first elements towards the understanding ofthe functionality ofthe potentially valuable, heat stable GH-57 enzymes, especially that ofthe T hydrothermalis ... hydrothermalis AL662 The analysis of this sequence revealed that the encoded enzyme is a member of GH-57 [21,22] The cloning and expression of apu in Escherichia coli has led to the production of...
... under the action of TPL gives evidence for the orthogonal orientation ofthe a-proton with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety of ... (c) the degree of restriction ofthe free rotation ofthe considered group in the active site For the reaction with L-phenylalanine the value of kr for nondeuterated substrate is more than for the ... group in the external aldimine defines automatically the positions ofthe a-proton and the side chain of any bound amino acid The lability ofthe a-proton observed for a large number of amino...
... how often they visited or walked by their first choice Once they had completed the ‘‘attractive’’ places section, participants were asked to place themselves on the other side ofthe aesthetic ... interviewed Exploring the generality of such a categorization in other geographical scenes, incorporating the analysis of places considered ‘‘unattractive,’’ is one ofthe aims ofthe present study ... evidence ofthe existence of this association in urban settings Within this framework, the overview guiding the design of this study is establishing categories of urban places from the perspective of...