the magnocellular neuronal phenotype cell specific gene expression in the hypothalamo neurohypophysial system
báo cáo khoa học: " Effects of DNMT1 silencing on malignant phenotype and methylated gene expression in cervical cancer cells" pdf
... protocol (Invitrogen Co.) Cells were harvested and rinsed twice in ice-cold PBS, and kept on ice for 30 in cell lysis buffer containing mM PMSF while agitating constantly, and insoluble cell debris ... to various degrees and the mRNA expressions were also increased These results are in accordance with the findings of other reports [15-19], which could be important in the development of new ... primer The relative changes in the extent of promoter methylation were determined by measuring the amount of promoter in immunoprecipitated DNA after normalization to the input DNA: %(MeDNA-IP/Input)...
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Báo cáo y học: "Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system" ppsx
... enriched in either embryonic or larval neurons In the future, it will be interesting to determine if these genes define stage -specific features of the developing nervous system The mRNA-tagging method ... the majority of genes in this interactome group are expressed in the nervous system favors the idea that these networks reflect authentic interactions in neurons We note that 13 of the proteins ... binding For example, the JIP3/JSAP1 JNK scaffolding protein, UNC-16, interacts with KLC-2 (kinesin light chain) to regulate vesicular transport in neurons [68] Other members of this interacting...
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báo cáo hóa học:" Site-specific analysis of gene expression in early osteoarthritis using the Pond-Nuki model in dogs" pot
... assessments The lamina splendens was not disrupted in any location based on lack of India ink staining, indicat- ing that AC surface integrity was maintained for two weeks in the dogs in this study ... level of expression for each gene (± standard error) compared to the house keeping gene GAPDH The increase in ADAMTS gene expression in the CrMFC approached significance and was included in the table ... may influence the "point of no return" in the progression of OA are chondrocyte viability and phenotype During the development of OA, there is often a focal increase in cell death[15-18] Since...
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Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
... current therapies In this study we provide the first gene array profiling of an estrogen responsive breast cancer cell line demonstrating that the combination of iodine and iodide alters gene expression ... BRCA1 inhibition thus permitting its inhibition of estrogen responsive transcription These data open the way for further defining pathways impacted by the essential element, iodine, in the cellular ... our hypothesis, we analyzed the effects of Lugol’s iodine solution (5% I2, 10% KI) on global gene expression in the estrogen responsive MCF-7 breast cancer cell line Analysis of the gene expression...
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Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... be narrower than the size distribution of the original population, cell size should increase as the cells move through the cell cycle, and during the period of cell division there should be a ... interest to look at the basic data in Table of Liu, Gaido and Wolfinger [1] In Table they present results on the gene expression amplitudes in two different experiments In Table they are trying ... explain differences between different experiments in terms of variation in the phase angles of peaking of gene expression in different individual cells Thus, given a particular variation in gene expression...
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Báo cáo y học: "Transporter Molecules influence the Gene Expression in HeLa Cells"
... protein linked to signal transduction (CD147) (GO:0007166) The basigin protein was shown to be present in vascular endothelium in both in the non-neoplastic region of the brain and in tumor cells ... impact on the gene expression patterns, when analyzing the efficacy and the genetic consequences of targeted gene therapy in vitro (and in vivo) Microarray Study Differential Gene Expression ... validated genes (A, B, C) The graphs display the results of the quantitative PCR of the differential gene expression related to the HPRT1 gene in HeLa cells 24h after treatment; the abscissae show the...
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Báo cáo khoa học: Oxygen control of nif gene expression in Klebsiella pneumoniae depends on NifL reduction at the cytoplasmic membrane by electrons derived from the reduced quinone pool doc
... tetracycline-resistance cassette (derived from the MiniTn5 [28]) was inserted and the resulting interrupted genes were cloned into the suicide vector, pKAS46 These constructs were then introduced into ... NifL-protein are located in the N-terminal domain [31] that binds the FAD-cofactor [17], one can speculate that the N-terminal domain of NifL might enter the lipid bilayer and contact the quinones ... derepressing conditions confirmed the observed nif – phenotype of both mutant strains In contrast to the parental strain, NifL was found mainly in the cytoplasmic fraction of the fdnG- and the nuoCD...
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Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
... intron of the ANX4 gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in the ... significant increase in the expression of the cyclin-dependent kinase inhibitor p21, a target of p53 [10] Comprehensive gene expression profiling has revealed that the pattern of gene expression in CCA cells ... RMG-II were maintained in Ham’s F-12 medium, and the other cell lines were cultured in RPMI medium The human embryonic kidney cell line HEK293 and the prostate adeno- 1480 carcinoma cell line LNCaP...
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báo cáo hóa học:" Progressive obesity leads to altered ovarian gene expression in the Lethal Yellow mouse: a microarray study" pptx
... This is the first report of differences in the levels of ovarian gene expression in an obese mouse model The most important finding of this study is that modified gene expression in the ovaries ... (in bold type) Genesto black mice involved in cholesterol biosynthesis exhibiting elevated expression (p < 0.05) in 180-day LY mice as comGenes (in bold type) involved in cholesterol biosynthesis ... by hyperinsulinemia/insulin resistance [37] Moreover, leptin bi-phasically modulates granulosa cell Star expression [36] An interesting and unexpected finding was the reciprocal shift in Hsd11b1...
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Báo cáo toán học: "A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3'''', 5''''-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pot
... lignin-degrading strain and for furthering our understanding of the lignin-degradation system in these fungi Phanerochaete chrysosporium, which is the most widely researched white-rot fungus in the world, ... is the entire ligninolytic system Information concerning the LiP and MnP expression system is highly important and requisite not only for better understanding the expression of the entire ligninolytic ... to interact with a CaM-oxysterol binding protein complex to signal the expression LiP and MnP in P 17 chrysosporium We will analyze possible correlations following the search for CaM-interacting...
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báo cáo hóa học:" A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pot
... play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and ... is the entire ligninolytic system Information concerning the LiP and MnP expression system is highly important and requisite not only for better understanding the expression of the entire ligninolytic ... expression as discussed in our previous report (Sakamoto et al 2010) Further study is required to determine whether the CaM has other functions including post-transcriptional effects on the expression...
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Báo cáo hóa học: "A calmodulin inhibitor, W-7 influences the effect of cyclic adenosine 3’, 5’-monophosphate signaling on ligninolytic enzyme gene expression in Phanerochaete chrysosporium" pptx
... play an important role in initiating the lignin degrading reaction of the fungus, because they can cleave lignin structures extracellularly in the first step of lignin mineralization (Cullen and ... is the entire ligninolytic system Information concerning the LiP and MnP expression system is highly important and requisite not only for better understanding the expression of the entire ligninolytic ... expression as discussed in our previous report (Sakamoto et al 2010) Further study is required to determine whether the CaM has other functions including post-transcriptional effects on the expression...
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Báo cáo y học: "The influence of the NOD Nss1/Idd5 loci on sialadenitis and gene expression in salivary glands of congenic mice" docx
... through the endothelial lining and into the SG [33] The reduced gene expression of Ifnz is of interest because the gene lies in the Nss1 congenic region The gene product is known to be inhibitory ... between the strains Gene expression differences of genes located in the NOD loci Idd5 and Nss1 The main focus of the gene expression profiling has been on identifying genes in the two NOD derived ... transforming the intensities, replacing missing values and collapsing probes to genes, the gene lists were filtered by minimum standard deviation = 0.7 Finally, the genes in the list 'pre-processing...
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Báo cáo y học: "Regulation of catabolic gene expression in normal and degenerate human intervertebral disc cells: implications for the pathogenesis of intervertebral disc degeneration" ppt
... factor-alpha on matrix gene expression The basal gene expression for the collagen type I gene was significantly higher in the degenerate cells than in the normal NP cells (P = 0.05) There was no significant ... NP cells (P = 0.05) and MMP-13 gene expression in normal cells (P = 0.05) (Figure 1a) There was no significant change in gene expression for MMP-9 in normal or degenerate cells or for MMP-13 in ... downregulated in normal NP cells following the addition of TNF-α (P = 0.05), but expression was not significantly changed in degenerate cells (Figure 2) There was no significant change in the gene expression...
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Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
... receptor binding parameters were determined using Scatchard analysis [32] The specific binding was deter- Page of 11 mined by subtracting non -specific binding from the total The binding parameters, ... activity in diabetic mice, but not in non diabetic mice [46] In our study, curcumin and insulin increased the dopamine D1 receptor expression levels in the cerebellum, which suggests that the curcumin ... hrs after the administration of morning dose of insulin and curcumin The results were expressed in terms of milligram per decilitre of blood Total Dopamine receptor binding studies in the cerebellum...
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Báo cáo y học: "Comparative effects of the herbal constituent parthenolide (Feverfew) on lipopolysaccharide-induced inflammatory gene expression in murine spleen and liver" ppt
... [8,10-12] in cell culture studies Parthenolide appears to inhibit NF-κB by targeting the IκB (inhibitor of NF-κB) kinase complex [9] which might inhibit proinflammatory cytokine and chemokine gene expression ... determine whether differences in expression levels of each gene existed between the spleen and liver These organs contain macrophages and other cell types capable of responding to LPS and other inflammatory ... liver cells and 80% of the liver's volume [18] The primary functions of these cells are exocrine and metabolic in nature Although they are capable of functioning as antigen-presenting cells in certain...
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báo cáo khoa học: " High-throughput sequencing identifies STAT3 as the DNA-associated factor for p53-NF-κB-complex-dependent gene expression in human heart failure" pps
... domain Purified recombinant GST-RELA peptides (amino terminus, DNA binding domain; carboxyl terminus, transactivation domain; in the left panel, the arrow indicates the GST-RELA 428-551 amino ... the cooperation between three transcription factors in a multi-protein complex may control gene expression program in heart failure Understanding the way that different DNA binding proteins interact ... of RELA because a purified recombinant GST fusion protein of the RELA carboxy-terminal domain, but not the amino terminus DNA binding domain, was sufficient to interact with p53 (Additional file...
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báo cáo khoa học: " The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro" ppsx
... nitrogen-containing bisphosphonates appeared to induce collagen type I gene expression during the first 10 days At day 14 the collagen gene expression level was lowered by the nitrogen containing bisphosphonates ... clodronate did not significantly influence the gene expression of cyclin D1 during the first days Zoledronate, however, caused a decreasing cyclin D1 gene expression after the 6th day whereas ibandronate ... explain the missing regeneration potential of BONJs In contrast, the non-nitrogen-containing bisphosphonate clodronate did not have any significant impact on osteoblasts cyclin D1 gene expression...
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báo cáo khoa học: " Uncovering the Arabidopsis thaliana nectary transcriptome: investigation of differential gene expression in floral nectariferous tissues" docx
... Comparison of gene expression in different nectary types Comparison of gene expression in different nectary types The number of genes displaying a two-fold or greater difference in expression in different ... within nectary-enriched genes The large numbers of genes displaying nectary-enriched expression profiles suggests common mechanisms for restricting and/or activating their expression within these ... significance of these elements occurring within the promoters of these genes Further information on the MYB4 and CArGCW8GAT promoter motifs are discussed below Discussion Gene expression profiles in different...
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báo cáo khoa học: " The SNF1-type serine-threonine protein kinase SAPK4 regulates stress-responsive gene expression in rice" ppsx
... SAPK4 in the regulation of gene expresModel Model on the putative involvement of the SNF1-type serine-threonine protein kinase SAPK4 in the regulation of gene expression in response to salinity ... deficient in the sucrose non-fermenting (SNF1) serine-threonine protein kinase that is related to the mammalian AMP-activated protein kinase was severely inhibited by NaCl indicating a main function ... kanamycin resistance and by the presence of the kanamycin resistance gene Plants of the T2 generations were used for further investigations to examine the role of SAPK4 in plant salt tolerance In...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25