... in the signals ofthe haem methyls of DvHc3:ZnRb complex vs the results in the isolated cytochrome is close to the full effect of complex formation Because the thermodynamic parameters for the ... contributions, hence they are suitable for monitoring the thermodynamic propertiesofthe cytochrome The paramagnetic chemical shift ofthe haem methyl resonances is a very sensitive probe ofthe haem environment ... respectively The figure shows that the effect of binding Zn-rubredoxin on the NMR signals ofthe haem methyls vs the results obtained for the isolated cytochrome is very small As observed for the case of...
... b sheet with the b12 ofthe other monomer, and towards the C-terminus, the subsequent residues, Phe182Lys186, pass through the a2 vicinity ofthe other monomer For the FeR dimer, the N-terminal ... Resolution ofthe structure of NADP+-bound FeR revealed that the nicotinamide moiety of NADP+ faces the re-side ofthe isoalloxazine ring, and that the 2Â-P-AMP moiety is held in the groove between the ... in the binding ofthe phosphate group(s) of FMN or FAD Lys92 forms a salt bridge with the O3P of FMN A salt bridge that involves avin species has not been reported in the structures ofthe other...
... destabilization ofthe overall structure ofthe sHsp [35,36] Therefore, we analyzed the effect of point mutations in the linker connecting the b5 and b7 strands of HSP22 on its thermal stability The wild-type ... correspond to the b8 strand ofthe other sHsp The primary structure ofthe a-crystallin domain of human sHsp is very conservative and the loop connecting the b5 and b7 strands is shorter than the corresponding ... affect the elution profile of any ofthe proteins analyzed The amplitude, position and the width ofthe protein peaks were not dependent on the transient heating These data suggest that the wild-type...
... residue to G143, the higher is the pKa value, independent ofthe nature ofthe displaced amino acid One possible explanation for the high pKa value of heme–Syn HO-1, and of Hmu O, is that the distal ... mg ofthe purified protein Formation ofthe heme–Syn HO-1 complex When an aliquot ofthe alkaline-hemin solution is added into the solution containing Syn HO-1, the resultant solution gives the ... Curve fitting ofthe fraction ofthe alkaline form to the calculated values using the Henderson–Hasselbalch equation yielded the best-fitted result with pKa ¼ 8.9 (Fig 5B) EPR spectra ofthe heme–Syn...
... suggests that cathepsin K is a new member ofthe kininase family Both the kininogenase activity of cathepsin L and/or the kininase activity of cathepsin K may be favored by the generation of an acidic ... components, which reduces the pH ofthe pericellular environment of macrophages [20] This raises the question ofthe dual behaviour of kininogens complexed with CP While concentrations of cystatins C, ... sites were identified, one at the Gly384Phe385 bond and the other at the Arg389-Ser390 bond, which is consistent with the unique ability of cathepsin K among mammalian cathepsins to accomodate Pro...
... Conclusions The present study indicates the importance of RGDechiHCit in the selective inhibition of endothelial aVb3 integrin Such inhibition opens new fields of investigation on the mechanisms of angiogenesis, ... in serum The RP-HPLC profile ofthe peptide before the incubation showed a single peak at tr = 16.64 min, ascribable to the complete sequence by the MS spectrum (Figure 1C) After 24h of incubation ... without the addition of hFN, they showed formation of only a few spontaneous tube structures (17.4 ± 1.2 branches per 10000 μm ) On the other hand, when the cells were plated on Matrigel with the...
... pA, while the black areas show a current lower than the noise level the conduction and trapping propertiesofthe stacks, which could reduce the reliability ofthe MOS devices due to the weaker ... strength ofthe grain boundaries Finally, the influence ofthe environment conditions on the study of polycrystalline high-k dielectrics was also analyzed The results demonstrate that the reduction of ... However, since the EOT ofthe polycrystalline sample is smaller (see Table 1), its lower conductivity can only be attributed to the crystallinity ofthe stack and not to the reduction ofthe oxide...
... towards the innards of these aggregates in which filler particles and the polymer matrix are present Nevertheless, the mechanical propertiesofthe filled samples were degraded after the absorption-desorption ... micrograph ofthe fracture toughness surfaces ofthe PMMA matrix at a magnification of 2000X Figure 4: SEM micrograph of fracture toughness surfaces ofthe 10 w% (BaTiO3-filled) PMMA matrix at 10000X The ... Effect of filler content on the fracture toughness ofthe PMMA matrix containing 10 wt% BaTiO3 3.2 The Effect of SBF Exposure on Fracture Toughness The mechanical propertiesof denture-base polymers...
... if ≤ i ≤ k , , otherwise The meaning ofthe existence of tB (i) is similar as in Remark 2.3 Proof of Theorem 1.2 We start with an outline ofthe proof First, we redefine the sequence B by adding ... implies the statement of Theorem 1.2 Proof of Theorem 1.2 In view of Lemma 1.5 we need to show that sC (2k) > 0.255 To this end, we will verify that the limit inferior ofthe right side of (3) ... take a sequence C of 2k symbols from the proof of Theorem 1.3, for k ≥ 12 and even Then an approach similar to the one used in Theorem 1.3 yields sC (2k) > 1− 2k + Proof of Theorem 1.4 For a...
... then converted to number of molecules, and the values for each sample were calculated as the ratio ofthe number ofmoleculesofthe target gene to the number ofmoleculesof GAPDH Data are expressed ... resorption The additive effect of both compounds at inhibiting bone resorptive activity could then be explained by the sum ofthe effect of CS on the OPG and RANKL and the effect of one or both of these ... osteoblasts, and the number of differentiated osteoclasts was measured with the tartrate-resistant acid phosphatase (TRAP) using the Bioquant software At the end ofthe incubation period, the cells...
... to the RNA, resulting in quenching ofthe tyrosine fluorescence We then studied the effect of changing the molar ratio ofthe viral RNA fragment to TGBp2 on the tyrosine fluorescence quenching ... property of TGBp2, which is responsible for the non-specific interaction between TGBp2 and viral RNA On the basis ofthe known topological propertiesof TGBp2 [9], we propose that the self-assembly of ... binding of TGBp2 To determine whether the unfused TGBp2 binds viral RNA in a specific or non-specific manner, two non-viral Figure The RNA-binding propertiesof unfused TGBp2 The RNA-binding properties...
... simplified the treatment of diabetes It is a competitive inhibitor ofthe major α-glucosidase enzymes in the brush border ofthe mucosal cell ofthe small intestine It inhibits the digestion ofthe ... modulator ofthe rate of insulin biosynthesis, through changes in mRNA levels (Kakita et al., 1982) Rat β-cells exhibit a differential regulation of biosynthesis ofthe two insulin isoforms at the ... Laboratory Officers, Ms Ng Foong Har ofthe Department of Biochemistry and Ms Annie Hsu, ofthe Department of Pharmacology and Ms Boon Yoke Yin, Laboratory Officer, Department of Biochemistry, for their...
... accurate positioning ofthe sample at the centre ofthe jaws, a correct amount of tightening ofthe jaw bolts was required – overtightening ofthe jaw caused buckling ofthe sample, while insufficient ... the name suggests, this method involves application of a measured acceleration pulse, or G-level, to the mounting holes ofthe board None ofthe other supporting structures such as the standoffs ... area ofthe gauge length The engineering strain in the sample is defined by L Lo (3.2) where L is the extension ofthe sample and Lo is the original length The true strain t, i.e the instantaneous...
... accurate positioning ofthe sample at the centre ofthe jaws, a correct amount of tightening ofthe jaw bolts was required – overtightening ofthe jaw caused buckling ofthe sample, while insufficient ... the name suggests, this method involves application of a measured acceleration pulse, or G-level, to the mounting holes ofthe board None ofthe other supporting structures such as the standoffs ... area ofthe gauge length The engineering strain in the sample is defined by L Lo (3.2) where L is the extension ofthe sample and Lo is the original length The true strain t, i.e the instantaneous...
... characteristics ofthe "Sassi of Matera" and "Trulli of Alberobello" In the South of Italy, the sites ofthe "Sassi of Matera" (Figure 1a) (classified as humanity world heritage in 1993) and "Trulli of Alberobello" ... if the present structures are characterized with local materials, namely, “Tufo of Matera” for the structures ofthe "Sassi of Matera," and “Stone of Fasano” for the structures ofthe "Trulli of ... and the knowledge ofthe temperature coefficient ofthe material of which it is made A single measure allows for the determination of diffusivity, thermal conductivity, and specific heat of the...
... time of heatsetting (air, solvent or water) and the tension applied to the substrates during heatsetting [11, 12] The essence of this paper is to determine the effect of time of heatsetting on the ... Figure Effect of time of heatsetting on the linearity of load extension in weft direction of knitted Ingeo™ Poly (lactic acid) Figure Mean effect of time of heatsetting on the linearity of load extension ... [PLA] Figure Mean [Ā] of effect of time of heatsetting on the tensile extension EM [%] of Ingeo™ Poly (lactic acid) 3.2 Linearity of load extension curve LT [-] The linearity of load extension curve...
... the SH sub-domain (item 1: ‘‘How often does your child have a lot of fun?’’ and item 4: ‘‘How often does your child feel happy?’’) The items ofthe SPS sub-domain Psychometric propertiesofthe ... smaller The RA and the PA sub-domains had lower correlations with their domains than most ofthe other domains at baseline, at endpoint, and Psychometric propertiesofthe CHIP-CE also for the change ... use the sub-domains rather than the domains ofthe CHIPCE when evaluating ADHD patients This is supported by the factor analysis based on the sub-domains and the correlation analysis ofthe sub-domains,...
... et al / VNU Journal of Science, Mathematics - Physics 25 (2009) 1-7 the magnetic squareness Mr/Ms reduced with J because ofthe presence ofthe Fe3Pt Of cause, the kink in the magnetic curves ... duration toff of 0.5 s During ton, FePt nanoparticles were deposited on the surface ofthe electrode When the current was switched off, an ultrasound was activated to remove the nanoparticles from the ... that of Pt which is similar to the XRD result of FePt foils prepared by cold deformation [20] The Pt peaks in the as-prepared samples are broad due to the small size ofthe particles Using the...
... of ferrites Ni1-xCoxFe2O4, hence it was the increasing of HC The increasing of HC proved that the soft magnetic property of ferrite NiFe2O4 changed to the hard magnetic property of ferrite CoFe2O4 ... atomic weights of all the atoms in the unit cell, V is volume ofthe unit cell and N is the Avogadro’s number Since each primitive unit cell ofthe spinel structure contains molecules, the X-ray density, ... materials, the second term can take part of several tens percent of HC and the last one whereas can only reach several hundreds of gauss Thus, the increasing of Co-concentration led to the increasing of...
... in the ECM, and the pH ofthe ECM control the filtering aspect ofthe ECM The ECM is a transit area for the passage of nutrients from the bloodstream into the cells and for toxins released by the ... improve the electrical functions ofthe ECM Therefore the composition and degree of toxicity ofthe ECM-glycocalyx interface will affect the electrical field and the flow of biocurrents in the ECM The ... between the inside ofthe cell and the outside ofthe cell The difference is known as the membrane potential • Because the membrane potential is created by the difference in the concentration of ions...