the effect of counter ions on the conformation of intrinsically disordered proteins studied by size exclusion chromatograph
Báo cáo y học: "Insights into the regulation of intrinsically disordered proteins in the human proteome by analyzing sequence and gene expression data" potx
... summary of expression profiles for the highly disordered proteins A summary of expression profiles for the highly disordered proteins (a) The heat map displays four distinct transcript groups; constitutively ... proportions of miRNA targeting and lower proportions of proteins being targeting for ubiquitination These properties play a role in the high levels of gene expression observed in the highly disordered ... highly disordered proteins compared to proteins with less disorder The regulation of disordered proteins is affected by the various factors studied, and the relationships between these properties...
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Báo cáo khoa học: Protein stabilization by compatible solutes Effect of diglycerol phosphate on the dynamics of Desulfovibrio gigas rubredoxin studied by NMR docx
... motions The effect is mostly in the longer time-scales and in the restriction of wider concerted motions, which encompass the structural reactions that allow protected amides to exchange with the ... increase the activation energy of the denaturing reaction would be an effective stabilizer This leads us to consider the possible effects of solute addition on the activation energy of the denaturing ... various conditions [3032] Hydrogen exchange rates of labile protons, such as the amide protons of protein backbones, can also provide dynamic information on longer timescales Amide protons that...
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The effect of inactivation for C.parvum by Pulse lamp
... Experiment results on run Fig Experiment results on run 2) Comparison of the lamps in turbid water samples The inactivation effects of the lamps on turbid water with the excystation and the cell culture ... show the experiment results on run and fig shows the experiment results on run 1) Comparison of the lamps using excystation evaluation Fig shows that the level of inactivity achieved with the ... Inactivation effect of the lamps using the cell culture method 3) Comparison of the evaluation methods used The experiment results (Fig 6) show that the sensitivity to UV was highest when the cell...
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Báo cáo hóa học: " Effect of Ebola virus proteins GP, NP and VP35 on VP40 VLP morphology" docx
... containing VP40+VP35, suggesting an organization of the core of the virus imparted by NP+VP35 nucleocapsid formations [15] The "bull's-eye" present in VP40+GP+NP VLPs along with the results of ... denser These findings suggest that the contribution of NP to density of the particle offsets the decrease in density by GP Statistical analysis suggests that the densities are within one standard ... VLP particles demonstrating the dimensions measured A Electron A Electron micrographs of individual VLP particles demonstrating the dimensions measured B Summary measurements of VLP Length, Diameter,...
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Báo cáo y học: "Effect of a lung recruitment maneuver by high-frequency oscillatory ventilation in experimental acute lung injury on organ blood flow in pigs" ppsx
... by the ventilator The esophageal catheter was then inserted up to the marked position into the esophagus The continuous measurement of the mean esophageal pressures started after activation of ... corrected either by volume administration or by the adaptation of the vasoactive drug dosage One possible explanation for the impairment in the hemodynamics is right ventricular dysfunction due to ... pressure-controlled ventilation (PCV) The primary objective of this study was to asses whether a recruitment procedure of the lung, at initiation of HFOV by stepwise increases of continuous distending...
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Báo cáo y học: "Effect of a lung recruitment maneuver by high-frequency oscillatory ventilation in experimental acute lung injury on organ blood flow" pdf
... by the ventilator The esophageal catheter was then inserted up to the marked position into the esophagus The continuous measurement of the mean esophageal pressures started after activation of ... corrected either by volume administration or by the adaptation of the vasoactive drug dosage One possible explanation for the impairment in the hemodynamics is right ventricular dysfunction due to ... pressure-controlled ventilation (PCV) The primary objective of this study was to asses whether a recruitment procedure of the lung, at initiation of HFOV by stepwise increases of continuous distending...
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Determination of EDTA in Water Samples by SPE-Gas Chromatography/Mass Spectrometry
... Wilmington, DE, USA) in electron-ionization mode with an ionization voltage of 70 eV and ion source temperature of 230ºC The instrument was operated in selected-ion monitoring (SIM) mode The monitor ... those of other river water samples collected downstream of STPs Because the STP is located on a tributary of Tsurumi River, the EDTA concentration might be affected by dilution with the influent of ... portion of Tama River Therefore, it was concluded that concentrations of EDTA decreased going downstream due to dilution In Kansai region, EDTA was only detected in the river water sample of Muko...
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Báo cáo khoa học: Catalytic transformations of supercoiled DNA as studied by flow linear dichroism technique doc
... present in the reaction mixture, only a certain 6340 Fig The effect of increasing concentrations of several campothecine analogues on the kinetics of topoisomerization of pUC19 DNA (8 lg) by topoisomerase ... glycerol) Fig Inhibition of topoizomerase II activity measured by the FLD technique Comparison of the kinetics of inhibition of reaction of lg of pUC19 DNA with lg of topoisomerase II by etoposide and ... transformations makes the above-mentioned consideration inapplicable Time-dependent topological transformations of the scDNA can be experimentally investigated by electrophoretic separation of DNA in the...
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Báo cáo sinh học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" ppt
... with the differentiation status of the KCs The close association of the HPV life cycle with the differentiation state of its host cell is demonstrated by the restriction of late gene transcription ... amplification of viral DNA to suprabasal epithelial cells The study of HPVs in cell culture has been hindered because of the difficulty in recreating the three-dimensional structure of the epithelium on ... pre-transfection treatment of methylcellulose also differentially regulated expression of the L1 proteins from PV Nat or Mod L1 genes, confirming further that expression of the L1 proteins from...
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Báo cáo hóa học: " Differential expression of papillomavirus L1 proteins encoded by authentic and codon modified L1 genes in methylcellulose-treated mouse keratinocytes" pptx
... with the differentiation status of the KCs The close association of the HPV life cycle with the differentiation state of its host cell is demonstrated by the restriction of late gene transcription ... amplification of viral DNA to suprabasal epithelial cells The study of HPVs in cell culture has been hindered because of the difficulty in recreating the three-dimensional structure of the epithelium on ... pre-transfection treatment of methylcellulose also differentially regulated expression of the L1 proteins from PV Nat or Mod L1 genes, confirming further that expression of the L1 proteins from...
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Báo cáo khoa học:" Properties of virion transactivator proteins encoded by primate cytomegaloviruses" doc
... expression of pp71 The basic vector, in1312, has mutations that inactivate the transcriptional functions of three crucial HSV-1 proteins The changes are an insertion in the coding region of the virion ... distribution of the protein whereas, by contrast, only 1% of pp71-positive profiles were dispersed The localizations of the other three homologs, B82, Rh82 and Ch82, were intermediate between those of ... additional level of transcriptional regulation, and one of the most extensively studied of these, the tegument phosphoprotein pp71 (encoded by HCMV gene UL82) activates expression from the MIEP...
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Báo cáo khoa học: " Properties of virion transactivator proteins encoded by primate cytomegaloviruses" pptx
... expression of pp71 The basic vector, in1312, has mutations that inactivate the transcriptional functions of three crucial HSV-1 proteins The changes are an insertion in the coding region of the virion ... distribution of the protein whereas, by contrast, only 1% of pp71-positive profiles were dispersed The localizations of the other three homologs, B82, Rh82 and Ch82, were intermediate between those of ... additional level of transcriptional regulation, and one of the most extensively studied of these, the tegument phosphoprotein pp71 (encoded by HCMV gene UL82) activates expression from the MIEP...
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IDENTIFICATION OF INTRINSICALLY DISORDERED SEPTAL PORE ASSOCIATED PROTEINS REGULATING DIVERSE ASPECTS OF
... cell types are connected to each other via gap junctions Gap junctions are composed of two connexons or hemichannels, with each cell contributing one Each connexon is a hexamer of connexin, a four ... A connexon can be composed of a single type of connexin (homomeric connexon) or different connexins (heteromeric connexon) Two identical connexons form a homotypic channel, while connexons containing ... are generally occluded in the centre by a plug (Ramus, 1969) The function of pit connections is not known, but the plugs that form in the centre of the pit connection act as temporary barriers...
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Selected aspects of enzymatic catalytic activity studied by theoretical methods
... from these applications indicates that the quality of the calibration does not depend on the charge-state of the iron centers, not on their spin state, not on their coordination number of the ... Formation of the right-hand sides of the CPSCF equations (eq (62)) Solution of the 3N CPSCF equations for the orbital rotation coefficients Uϑσ ( ) λ Addition of the H FRHS , Uϑ terms to the Hessian ... the protonation state of one of the residues does affect the strength and position of the H-bonds formed by the others In the Y10F mutant His369 is closer to the NO, whereas mutation of both distal...
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Tài liệu Báo cáo khoa học: Effect of ionic strength and oxidation on the P-loop conformation of the protein tyrosine phosphatase-like phytase, PhyAsr docx
... unoxidized conformation Oxidation of the catalytic cysteine decreased the contacts made to the cysteine Sc but resulted in the formation of 17 additional interactions with the oxygens of the cysteine ... conformations To accommodate the larger size of the cysteine sulfonic acid, the P-loop must undergo a conformational change In the absence of the large P-loop movement, ˚ the main chain amine of Gly255 ... and distinct conformation rearrangement regardless of the position of the general acid and active site arginine In summary, the conformation of the P-loop only changes: (a) when the general acid...
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Báo cáo khoa học: Effect of magnesium ions on the activity of the cytosolic NADH/cytochrome c electron transport system pptx
... catalysed by the respiratory chain is completely different from their effect on the oxidation of exogenous NADH Fig Effect of magnesium ions on both the content of endogenous cyto-c (A) and the binding ... stimulatory effect depending on whether it is added to the incubation medium before or after the mitochondria The plurality and differential effects of magnesium ions on the activity of the respiratory ... added after the mitochondria It also provides direct evidence that, in these latter conditions, the addition of magnesium mimics the results obtained with the addition of cyto-c, the only difference...
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Báo cáo khoa học: " Effect of the medical emergency team on long-term mortality following major surgery"
... swept into the mangroves behind the shores by the force of the wave, causing polymicrobial infections [1,5] Repeated multilocal microbiology identified a wide spectrum of bacteria common to the marine ... estimation about the force with which the victims were hit and swept away by the wave Antiinfective therapy Our intial choice of anti-infective therapy was a combination of a potent quinolone combined ... Psychoemotional aftermath With respect to the tsunami's psychoemotional aftermath, the full impact of the wave on the mental health of the survivors is still unknown [2] In February 2005 the World...
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Báo cáo y học: "Effect of antibodies on the expression of Plasmodium falciparum circumsporozoite protein gene"
... deviation of the mean No amplification was observed when cDNA controls, that lack the reverse transcriptase enzyme, were used in the real time PCR reactions, demonstrating that there was no gDNA contamination ... contamination PCR reaction triplicates showed no major differences among them and expression levels of the internal control were similar in the different experiments The expression levels of csp ... parasite development in the sporogonic [3 - 5], exoerythrocytic [6] and erythrocytic [7] cycle However, there is almost no information on the effect of antibodies on the expression of Plasmodium immunogenic...
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THE EFFECT OF EDUCATION ON ECONOMIC master sciences po fr
... fact of considering only one of the channels in the regression (either the change, which given the MASTER THESIS: THE EFFECT OF EDUCATION ON ECONOMIC GROWTH negative correlation, would lead to ... male individuals go in the same direction than with the whole population, confirming thereby my previous results Conclusion The literature on the effect of education on economic growth is far from ... specially the definition of human capital In order to measure the impact of this misspecification, the authors analyze two specifications for the regression of economic growth on human capital : • One...
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EFFECT OF THE NUMBER OF THE VERTICAL PIPES FOR THE PASSIVE AERATION ON THE COMPOSTING RATE
... based on the ratio of carbon loss as CO2 to the carbon contained in the DF alone In our investigation, the concentrations of CO2 from about to 19% in the exhaust gas were detected during each of the ... ventilation effect by the natural convection (Shimizu and Morita, 1962) However, there have been no studies of the effect of the vertical pipes on the passive aeration and the composting rate The ... respectively CONCLUSIONS The effect of the number of the vertical pipes for air supply in the composting pile on the composting rate was investigated by measuring the bed temperature variations and the...
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