... contrast, the chimeras possessing only the mouse HSF3 activation 4116 domain did not induce their expression Furthermore, the C-terminal activation domain of human HSF1 [43–45] was swapped with the ... HSF1 [19] These observations implied that a balance of the monomer and trimer HSF affects the amount of HSF bound to the HSE in vivo, but even a small amount of the trimer could regulate the gene ... inducing the expression of only nonclassical heat shock genes These observations suggest the importance of the regulation and function of the nonclassical heat shock genes Analysis of these new...
... microscopy study of the neuronal endings in the sinus gland to determine the subcellular localization of the different epimers Taken together, these results allow a discussion of the existence of ... within a well-defined and restricted peptide family, as illustrated by a recent study of the I1-conotoxin superfamily [35] In the case of the CHH family, the occurrence of DAACPs appears to be problematic ... terminal in the sinus gland, as a result of late and progressive isomerization of the Phe3 of the CHH during the migration of the secretion vesicles along the axonal tract [39,41] The immunohistochemical...
... [3,11–13] Of the three GH families of the clan GH-H, it was thefamily GH13 that was originally established as the a-amylase family [14–17] At present it belongs to the largest families in the entire ... not have it [9,19,24,25] The main goal of the present study was to investigate further the resemblance between hcHAT proteins and the enzymes from the a-amylase family We therefore carried out a ... of the hcHAT proteins and the GH13 a-amylase family members The tree is based on the alignment of complete sequences and calculated including gaps The numbers represent the bootstrap values The...
... I The Teresiani and the Prussiani II Frederick the Great as a Saint III The Cloister Brothers of San Giovanni e Paolo IV The Return from the Army V The Brave Fathers and the Cowardly Sons VI The ... Prison Barricade VII The Battle of Collin VIII The Inimical Brothers IX The Letters X In the Castle at Dresden XI The Te Deum XII Camp Scene XIII The Watch-Fire XIV The Battle of Leuthen XV Winter ... Wife IV The Fete in the Woods V Intrigues VI The Private Audience VII The Traitor VIII Declaration of War IX The King and his Brothers X The Laurel-Branch XI The Ball at Count Bruhl's XII The Interrupted...
... Yu et al therapeutics First, the relatively small size of the enzyme family can make it easier to specifically target the enzyme of interest and distinguish it from other members of thefamily Second, ... extra-enzymatic abilities (Fig 1) The DPIV gene familyThe DPIV gene family is a subgroup of the prolyl oligopeptidase family of enzymes (Table 1), which are specialized in the cleavage of prolyl bonds ... denotes the location of the active site Some residues in front of the figure, which would otherwise obscure the active site, have been omitted to indicate the hollow cavity found in this protein family...
... respectively The TTR family branch represented by vertebrates is also indicated vertebrates The considerable sequence similarity at the binding site within the TRP family indicates that they perform ... comparing the sequence of the amplified cDNA of R09H10.3 to that of the available genomic sequence, we were able to deduce the organization of exons and introns The splicing between the two protein ... The 5¢ splice site used for splicing of the SL1 trans-spliced leader sequence (0) and the position of the 3¢ primer used in RT/PCR and sequencing are indicated The structure and sequence of the...
... explains the impact of the protein environment on the stereoselectivity of the cyclization reaction As a result of the steric restrictions in the cavity, changes in the position of the epoxide ... stereoselectivity of the reaction (Table [23]) To further characterize the importance of the hydrophobic environment inside the binding pocket of AOC2, and to assess the role of the amino acids forming the polar ... around the C8–C9 bond The conformational change is further promoted by a hydrophobic effect, as it buries more of the hydrocarbon tail of the molecule inside the cavity In contrast to the spontaneous...
... located on the SBD on the side opposite to the variable loop region and the catalytic domain, whereas binding site is near the catalytic domain This juxtaposition of the SBD relative to the catalytic ... challenging The corresponding evolutionary tree (Fig 2), containing 33 sequences from the largest family, CBM20, eight from CBM21, 16 from CBM48, and three from the new family, CBM53, highlights the ... distort the conformation and packing of the polymers, thereby facilitating their degradation CBMs with afnity for starch are commonly referred to as SBDs The rst discovered SBDs were placed in the...
... of the GATEWAY cassette The initiation codon is therefore provided by the vector, and is the same for all the constructs The 5¢-flanking region of each cardosin gene was amplified by PCR from the ... a The artificial mismatch introduced in the primer is underlined b The att sequences are underlined c The ATG within the reverse primer, mutated to ATC, is in Italic d The six bases preceding the ... k4.2 The former harbored the complete sequence of the cardosin B gene, whereas the latter enclosed a partial sequence of the cyprosin gene Like other plant AP genes, cardosin genes have their...
... within the two ZAS Fig Structural organization of the XHIVEP proteins Black bars indicate the position of the ZAS C2H2 zinc fingers (ZAS-N and ZASC) and the isolated C2HC zinc finger (I-Znf) The ZAS ... has, at its core the invariant CCC and an upstream GAGA box To evaluate the contribution of these elements to ZAS-N Znf binding, mutations were created in either the GAGA box or the trinucleotide ... representation of the full length XHIVEP1 and the Myc-tagged (MT) deletion mutants Black and white boxes indicate the position of the Znf domains and the serine-rich stripe, respectively The relative...
... the two members of the cinnamyl alcohol dehydrogenase family (a subdivision of the MDR superfamily [22]), in yeast This enzymatic family probably participates in the lignin synthesis pathway in ... amplify by PCR the truncated gene carrying the URA3 marker The yeast strains BJ2168 and BJ18 were transformed with the truncated ycr105w gene by using the lithium acetate method [19] The resulting ... 7.0) These results and the specificity for NADP(H) suggest that the enzyme would act as an aldehyde reductase, rather than as an alcohol dehydrogenase The catalytic efficiencies shown for the reductive...
... because, among other things, it didn’t have the power to hire or fire the ground crew, set the pay rates for the workers, keep their employment records, set their work schedules, or control their work ... between the two companies to determine whether your company acts like an employer The main factors to consider are whether your company: • has the power to hire and fire some employees of the other ... other company • has the power to set rates and methods of pay of employees of the other company • has the right to supervise the work and work schedules of employees of the other company, and •...
... basically thefamily s task to sustain itself, with the able-bodied providing for the disabled, the elders educating the young, the healthy caring for the sick, the young providing security for the ... value of the conceptions of thefamily and proper family relations in the three Abrahamic religions and will leave to others the question of whether the resultant familial institutions in the Western ... clinching their point In sum, then, the reader of the Classic of Family Reverence needs to keep in mind both the routine life of the people and the rapid pace of change and violence endemic to the...
... As the pada lacked a common space, the girls and the mothers groups met in the house of the pada worker, and the boys and fathers groups met in the house of the angan- http://www.jiasociety.org/content/12/1/35 ... deter their participation in a familybased programme In the mother focus groups, some women indicated that although they wanted to talk about HIV/AIDS with their children, they were worried that their ... transmitted, others indicated that they knew very little In the mother focus groups, one mother explained her knowledge about HIV/AIDS as: Of the boys who had some knowledge about HIV/AIDS, they identified...
... within the pannus, which are the putative target of this cytokine [18,19] The localization of Ang-1 is similar to that of VEGF, which further supports the crucial role of the interaction between these ... (Ang-1) in synovial tissues The arrows denote the lining cell layer, the double arrowhead denotes subsynovial macrophages and the single arrowhead denotes vascular endothelium The immunohistochemistry ... constitute a family of endothelial tyrosine kinase receptors [3,4] There are two members in this receptor family, termed Tie1 and Tie2 (also known as Tek) Tie1 is an orphan receptor, whereas the ligands...
... mRNA induction The level of BAFF mRNA peaked 48 hours after the addition of IFN-γ (Figure 1b) Subsequently, these positive results in epithelial cell lines led us to determine whether they could ... the T cell infiltrate and ductal epithelial cells [29] However, we could not eliminate the possibility that this finding was due to the passive fixation of BAFF on its receptor Glandular epithelial ... in epithelial-cell cultures from patients and in the HSG cell line, as seen by the total absence of staining with myeloid markers in immunocytochemistry and the 100% positivity with epithelial...
... thus hypothesized that some of the six new members of the IL-1 family might also play a role during RA Therefore, we sought to investigate the effects and the expression of new IL-1 family members ... [13] In the present study we investigated the effects of the new IL1 family member IL-1F8 on primary human synovial fibroblasts (hSFs) and human articular chondrocytes (hACs), and examined the expression ... play key roles in mediating the pathophysiological processes that underlie the inflammation and tissue destruction that occur in RA The role of the four 'classical' IL1 family members (for instance,...
... level to the GS genes in archaebacteria The full-length cDNAs (FLcDNAs) of the GS genes were analyzed and the characteristics of the polypeptides encoded by their ORFs were compared (Table 1) The ... individual members of the gene family confirming the existence of the GS gene duplicates The four duplicated GS genes were positioned in the linkage groups (LG) or scaffolds present in the Populus trichocarpa ... PtGS2 duplicates On the other hand, the last exon in the PtGS1.1 and PtGS1.3 duplicates represents the fusion of exons 12 and 13 in the PtGS1.2 duplicate It is interesting to note the presence of...
... TIP1;1 in the knockout On the other hand, the effect of the absence of TIP1;2 from the root cap may be subtle and may have gone undetected under the experimental conditions adopted for the whole-plant ... reach the tonoplast This is further demonstrated by the fact that in the epidermis of cotyledonary cells, where TIP1;2 is uniformly expressed, the fusion protein appears to localise to the tonoplast ... detectable in 2-4 cells at the LRP As the LRP grows further, the number of cells expressing TIP2;1-YFP increases but remains confined to a cluster underlying the base the lateral root (Fig 4, panels...