the cartesian coordinate system is formed by the

Ngày tải lên: 18/06/2014, 17:20

... proteins within the cell. We found this translocation to be dependent on the final amino acids of the receptor protein. Similar to the other PDZ domain interactions that have been shown to be abolished by ... signal is impaired. We finally examined the effect of deletion of the components of the PDZ binding motif on the signal- ing properties of the receptor in Ca 2+ imaging experiments. Truncation of the ... summary, when the interaction between MUPP1 and OR2AG1 is inhibited by over-expressing the interacting PDZ domains, the resulting response of OR2AG1 to odorant is modified in that the rapid decay...

Ngày tải lên: 18/02/2014, 13:20

... Fenton reaction with the iron metal at the active site. The presence of iron is therefore a prerequisite for its effect and suggests that the absence of reactivity with the MnSOD mutants is due to the lack ... identified to distinguish between FeSOD and MnSOD [7]. The position of the glutamine residue, which is structurally equivalent in the enzymes, is contributed by the N domain of FeSODs (Q69) and the C ... a shift in the position of Y34 and a twist in the ring of the metal- binding H81. Thermostability is not a new phenomenon amongst SODs; those isolated from hyperthermophilic organisms, for example,...

Ngày tải lên: 21/02/2014, 01:21

... Subsequently, the GST–PP2Aca:His–TIPRL complex was incubated with His–a4 and the GST–PP2Aca:His–a4 complex was incubated with His–TIPRL. Binding of His–TIPRL to the previously formed GST–PP2Aca:His–a4 ... order to test this hypothesis, in vitro assays were performed in which the activity of PP2A core enzyme (A and C subunits) was measured in the pres- ence of His–a4 or His–TIPRL using the phosphopep- tide ... PP2Ac is represented by a black bar for comparison. Numbers on the left of the gray bars indicate the first amino acid in the respective activation domain-phosphatase catalytic subunit fusion. The...

Ngày tải lên: 07/03/2014, 05:20

... recruitment, due to the absence of the NTD. This might be overcome by the overexpression of cofactors. To test this hypothesis, we analyzed the effects of two AR cofactors known to interact with the AR LBD. ... observed. The important role of the NTD, which is absent in AR45, has been documented by several studies [40–42]. The hormone-dependent interaction between this region and the LBD of the AR is essen- tial ... primer specific for the extreme 5¢ region was then used together with a primer recognizing the 3¢ end of the AR coding part to amplify the complete coding region of this novel AR form. The deduced amino...

Ngày tải lên: 07/03/2014, 16:20

... mutagenesis of the N-terminus of the V 1a R; this revealed that a single residue (Arg46) located in the distal segment of this domain is critical for high affinity agonist binding but not antag- onist ... affinity agonist binding, by site- directed mutagenesis. The wildtype Arg46 was systematic- ally substituted by all the other 19 encoded amino acids. These receptor constructs were then characterized ... rescued by a chimeric construct in which the N-terminus of the OTR is replaced with the corresponding sequence of the V 1a R [10]. The role of the N-terminus has been addressed recently by alanine-scanning...

Ngày tải lên: 07/03/2014, 21:20

... see [11,12]). At the renal epithelium, small peptides are reabsorbed from the glomerular filtrate into the cells by the subtypes PEPT1 and PEPT2. PEPT2 is also expressed in other tissues, such as ... insertion of the correct cDNA was verified by sequencing. The pNKS-hPEPT1 construct served as template for cRNA synthesis. After linearizing the plasmids with NotI, cRNAs were synthesized using the mMESSAGE ... of vertebrates [1,2]. It is formed as a secondary product through the methylation of the dipeptide carnosine (b-alanyl-l-histidine) [3]. The reac- tion is catalysed by carnosine N-methyltransferase [3,4]....

Ngày tải lên: 15/03/2014, 09:20

... and obviously the isolated proteins are degraded by the protease. On the basis of these observations, we can explain not only the protective effect exerted by HSP90 on the digestion of NOS isozymes, ... can prevent the degradation by calpain of both NOS by protecting the cleavage of the peptide bond close to the CaM binding site. This explains why both NOS are inactivated by calpain in the absence ... quantified as described in the Exper- imental procedures. The areas of the peaks were normalized on the basis of the amount of protein loaded on the column. The val- ues reported are the arithmetical mean...

Ngày tải lên: 23/03/2014, 07:20

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 30/03/2014, 04:20

Ngày tải lên: 31/03/2014, 21:21

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 19/06/2014, 22:20

Ngày tải lên: 20/06/2014, 23:20

... is thus a theory of the global distribution of the units of the grammar. The English grammatical rank scale recognized by Systemic Functional Grammar is as follows: In turn, each unit has their ... English Theme is the first element of a clause and providing what we call the departure for the starting point for the clause. By changing Theme we do not change the ideational meaning but the ... what is it that the participants are engaged in, in which the language is an essential component? + The TENOR OF DISCOURSE concerns who is taking part, to the nature of the participants, their...

Ngày tải lên: 07/11/2012, 14:44

... Safe Senders List The Safe Senders list is part of the Junk E-mail filters. If you choose to allow external content from addresses listed in your Safe Senders list, you need to check the configuration ... select Add Sender to Safe Senders List or Add the Domain @[domain-name] to Safe Senders List. The sender's fully qualified domain name is listed in the menu selection, as shown previously ... you need to check the configuration of the list. You can add addresses and domains to the Safe Senders list directly from an email message. Click on the InfoBar or right-click in any image...

Ngày tải lên: 21/01/2014, 02:20

... as the disease became less prevalent; however, the risk is still high for HCWs who work in settings in which the incidence of TB among patients is high. The precise risk for TB among HCWs in the ... developed at the University of Illinois (Chicago, Illinois) from a strain originated at the Pasteur Institute. The Food and Drug Administration is considering another vaccine, which is produced by Connaught Laboratories, ... with the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices vi MMWR April 26, 1996 Advisory Council for the Elimination of Tuberculosis (ACET) September...

Ngày tải lên: 15/02/2014, 13:20

... tyrosines, by the PTK Lck, to be deduced. Experimental procedures Peptide synthesis Peptides were made by manual solid-phase peptide synthesis using the tBoc method [11], purified by RP-HPLC, ... sample. The analysis was repeated for fragment 5–6, the other high-yielding product of the (incomplete) trypsin digest in which the only ITAM Tyr was 1N. This procedure was performed for all of the ... 2, and the farthest as 3. The two tyrosines within each ITAM are then further classified as N or C, reflecting their locations relative to the N-terminus and C-terminus (Fig. 1). Six synthetic...

Ngày tải lên: 08/03/2014, 02:20

... level of GR in the epithelia of the epididymis (Fig. 1 ). As the epididymis is rich in sulfoxidase, the oxidation of sulfhydryls in the sperm head and tail would be mediated by this enzyme in a ... Thiol oxidation occurs in the nuclei and the tail during the maturation process in the epididymis. The greater the extent of oxidation in nuclei, the more potent are the spermatozoa. In addition, ... prostate gland. The l ocalization of GR, as evidenced by immunohistochemical techniques, reveals that it exists at high levels in the epithelia of the genital tract. In testis, GR is mainly localized...

Ngày tải lên: 17/03/2014, 17:20