tối ưu hóa điều kiện ly trích polysaccharide từ bột nấm linh chi đã qua giai đoạn tiền xử lý
Folk medicine cratoxylum cochinchinense antioxidant but cytotoxic
... (Cratoxylum cochinchinense, Cortex magnoliae officinalis, Psoralea corylifolia L, Curculigo orchioides Gaertn, and Glycyrrhiza uralensis Fisch.) were selected for further characterization C cochinchinense ... University of Singapore for supplying us the Chinese medicinal extracts This project would not be possible without these extracts, especially Cratoxylum cochinchinense which was harvested from ... corylifolia L showed a higher activity C cochinchinense was particularly potent in inhibiting the formation of advanced glycation end products on proteins and strongly inhibited hypochlorous acidinduced...
- 200
- 289
- 0
Isolation, purification and detection of soyasaponins and their associated bioactivities in cultured hepatocarcinoma cells
... technical expertise towards my progress have truly been incredibly helpful Especially, he used his experience to make me understand how to be a qualified PhD student, how to be a good researcher ... flour 94 7.2.2 Hydrolysis at different temperatures 94 7.2.3 HPLC-ESI-MS Analysis 94 7.2.4 Capillary Temperature Optimum of ESI-MS analysis and MS-MS (MSn) analysis ... represents the amount before hydrolysis, open bar represents hydrolysis in a closed container in a 80° water bath, hatched bars was hydrolysis at 100° and solid bar was hydrolysis in a C C 120° dry oven...
- 147
- 280
- 0
Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
... designated as EHEChemolysin, coded by the EHEC hly operon There are two different plasmid-encoded hemolysins, both members of the RTX toxin family, have been described for STEC Alphahemolysin is formed ... easily Recently, immunomagnetic particles for the separation of E coli O26 and O111 have become commercially available With the use of IMS, the isolation rate of E coli O157 has been markedly ... respectively 10 Ji-Yeon Kim et al (Nasco, USA), and placed into the icebox Upon arrival at the laboratory, samples were either analyzed immediately or held at 4oC for no longer than 24 h before analysis...
- 13
- 456
- 0
Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt
... (TEM, JME-100EA III; JEOL, Japan) Additionally, ultrathin sectioning of cultured cells was used to examine inclusion bodies with reference to a previously described method [8] The neutralization ... using the method of Reed-Muench [1] The neutralization test was performed as previously described [7] Briefly, FCWF cells were grown in 96-well flat-bottomed plates until a monolayer of cells ... recommendations and then sequenced The sequence was analyzed by GENETYX version 9.0 computer software (Software Development, Japan) and DNAMAN version 4.0 (Lynnon BioSoft, Canada) Bacteriology failed to...
- 3
- 308
- 0
Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx
... inhibit the expression of Epstein-Barr virus (EBV) lytic proteins during the viral lytic cycle in P3HR1 cells, an oral lymphoma cell line latently infected by EBV Andrographolide inhibits the production ... BW) [101] blood glucose in STZ-induced hyperglycaemic rats (50 mg/kg BW, 10d) [115] hepatic CAT, SOD and GST activities in lymphoma bearing mice (orally 10~30 mg/d) [123] d: day; BW: body weight ... tumor in melanoma subcutaneously implanted mice (orally 200, 400 mg/kg BW, 10d) [45] Immunomodulation proliferation and IL-2 induction in hPBL [108] Anti-hyperglycemic effect plasma glucose concentrations...
- 15
- 311
- 1
Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents
... Ixeris sonchifolia Hance Ixeris sonchifolia Hance (Fig.1.2.1), which belongs to the Compositae family, is a small and bitter perennial herb widely distributed in the northeastern part of China (Feng ... sonchifolia Hance is known for its fibrinolytic and thrombolytic activity, another objective of this thesis was to test if the potential anti-stroke effect of Ixeris sonchifolia Hance was partly ... Calibration graphs 60 3.1.2.4 Quality control 60 3.3 Results 61 3.3.1 Characterization of purified compounds isolated from Ixeris sonchifolia Hance 61 3.3.2 Quantitative HPLC analysis of compounds to...
- 188
- 422
- 0
isolation, selection and identification bacillus subtilis from muddreg of beer
... EXAMINATION COMMITTEE Abstract Bacillus subtilis had widely used in many fields such as medicine, food processing, veterinary, aquaculture and especially in environmental treatment It helps disintegrate ... is approximately seconds Put a counterstain, such as basic fuchsin solution, on the slide over the bacterial smear Allow the counterstain to remain on the smear for approximately 40 to 60 seconds ... Thơ Nguyễn Đức Lượng Nguyễn Thị Thùy Dương 2003 Công nghệ sinh học môi trường, tập 1, Công nghệ xử lý nước thải, Nxb Đại học Quốc gia Tp Hồ Chí Minh, tr.223-225 Nguyễn Lân Dũng 1997 Vi sinh vật...
- 29
- 299
- 0
Purification and cloning of PCR products
... cloning only works efficiently with thermostable DNA polymerases, such as Taq DNA polymerase, which have terminal transferase activity However, for many PCR cloning purposes Taq DNA polymerase ... sample can usually be concentrated by evaporation for a few minutes in a spin-vac to achieve the desired concentration Ethanol precipitation Ethanol precipitation can be used as a fairly crude purification ... is performed the agarose may partly solidify, but this is not a problem Advantages of in-gel ligation are that it is rapid and relatively cheap (although high-quality agarose is expensive) and...
- 26
- 555
- 0
Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf
... why they are typically maintained predominantly in either the highly methylated or the highly demethylated state This is because any repressive modification is likely to be rapidly removed if it ... in Chironomus tentans provide another representation of very actively transcribed genes These are looped out domains of highly decondensed chromatin containing genes heavily loaded with polymerases ... models of chromatin depict chains of regularly spaced nucleosomes that fold up into a helix as highly ordered chromatin fibres However, this image is really only representative of inactive loci that...
- 29
- 743
- 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... respectively This suggests that in the B circulans and the Alteromonas sp chitinases a negatively charged catalytic aspartate residue is absolutely essential, while in the hevamine and A caviae chitinases ... in catalysis is the strict conservation of this residue in family 18 chitinases [28,33] Moreover, mutation of the homologous residues resulted in strongly decreased activities of the chitinases ... the chitinases mentioned above Unfortunately, as yet no structural information is available on those chitinases to support this notion Role of Asp125 in catalysis Information on the catalytic...
- 9
- 616
- 0
Báo cáo " Analysis and identification of multi-variate random pressure fields using covariance and spectral proper transformations " pdf
... analysis of mean and fluctuating pressure fields around low-rise building directly measured due to turbulent flows A linkage between pattern of the pressure distribution and POD modes, especially ... modes observed differently in two cases Kikuchi et al 1997 [6] applied the POD to pressure field of tall buildings, then fluctuating pressure field was reconstructed due to only few dominant POD ... function, suitably normalized due to the mean square basis [1]: Max | ( p(υ , t ) ⊗ Φ (υ )) |2 Φ (υ ) (3) where (⊗ ) , , , denote to inner product, expectation, absolute and Euler squared norm...
- 14
- 390
- 0
Báo cáo sinh học: "Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" pot
... glycoprotein EEV glycoprotein Profilin homolog Interact with host IL-1 EEV glycoprtn, hemagglutinin EEV glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early ... for analysis, and helped analyze the mass spectrometry data TSC prepared samples for analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis ... no general analytical strategy available that is capable of identifying membrane and core proteins, low and high abundant proteins equally well Therefore, we have used several analytical strategies...
- 16
- 331
- 0
báo cáo hóa học:"Pox proteomics: mass spectrometry analysis and identification of Vaccinia virion proteins" docx
... glycoprotein EEV glycoprotein Profilin homolog Interact with host IL-1 EEV glycoprtn, hemagglutinin EEV glycoprotein Serpin (C16L) Capping enzyme subunit virion component virion component Early ... for analysis, and helped analyze the mass spectrometry data TSC prepared samples for analysis, analyzed the mass spectrometry data and helped to draft the manuscript SV assisted with the analysis ... no general analytical strategy available that is capable of identifying membrane and core proteins, low and high abundant proteins equally well Therefore, we have used several analytical strategies...
- 16
- 455
- 0
báo cáo hóa học:" Characterization of the tumor marker muc16 (ca125) expressed by murine ovarian tumor cell lines and identification of a panel of cross-reactive monoclonal antibodies" docx
... only slightly retarded on this column (Fig 2) and was initially identified (data not shown) by Alcian Blue staining [16] To specifically identify Muc16, we conducted Western blot analysis of Pool ... analyses The widely employed clinical serum CA125 assay was also unable to detect CA125 in spent media of MOVCAR-2 cells We therefore tested a panel of ten anti-MUC16 antibodies that we recently ... approximately 250 kDa band for Muc16 (Fig 3) The binding to Muc16 was weaker as compared to MUC16 and was usually observed at 1:250 dilution of the primary antibodies 618F and 653F specifically recognize...
- 7
- 430
- 0
Báo cáo hóa học: " Purification and characterization of novel fibrinolytic proteases as potential antithrombotic agents from earthworm Perionyx excavatus" pot
... and remarkably strong firbinolytic enzymes from Perionyx excavatus earthworm (family Megascolecidae) This species has been widely cultivated in Southern Vietnam for the production of aquatic animal ... by a 15-day autolysis at room temperature Consequently, a two-fold increase of caseinolytic activity was achieved after 10 days Precipitation of earthworm proteins from the autolysate by acetone ... (USA) Page of 11 Autolysis of P excavatus Earthworm biomass was washed and finely homogenized in distilled water containing 0.1% (w/v) sodium azide Autolysis was initially performed at 45°C for...
- 11
- 375
- 0
Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... ecneuqes eht etelpmoc ot tuo deirrac eb lliw slairt eroM etis noitalysocylg laitnetop eno ylno htiw nietorp enarbmemsnart detalysocylg a si nietorp largetni sihT )%55( sullag sullaG dna )%06( ... ytiniffA giF gm 526.0 saw detule snietorpocylg lavral fo tnuoma latot ehT )3 giF( edisonirypoculg D-α lyhtem M 2.0 htiw detule erew hcihw ,snietorpocylg lavral eht yfirup ot esorahpeS-AnoC yb deifirup ... lareveS sutalunna B sulihpooB la te mikaH lE E rmA 671 .h/lm 03 fo etar wolf a ta edisonirypoculg D-α lyhtem M 2.0 htiw detule erew snietorpocylg dnuob eht dna ,4.7 Hp ,lCaN M 5.0 ,lCH-sirT Mm 02 gnisu...
- 6
- 267
- 0
Báo cáo y học: "Genome-wide prioritization of disease genes and identification of disease-disease associations from an integrated human functional linkage network" pps
... diseases still have an AUC of over 0.75, 0.8, and 0.9, respectively Additionally, we have also performed the disease centric analysis using only the area of the extreme left side of the ROC curve, which ... middle, and top lines of the box represent the first quartile, the median, and the third quartile, respectively; whiskers represent 1.5 times the inter-quartile range; red plus signs represent outliers ... predictions not solely rely on the potentially biased text mining, molecular function and cellular component annotations of GO, and curated PPI data sources; other relatively unbiased sources...
- 17
- 415
- 0
báo cáo khoa học: " Screening and Identification of a Renal Carcinoma Specific Peptide from a Phage Display Peptide Library" pot
... contributed equally to this work § Corresponding author TXA: txabs9988@163.com ZJT: brianzg86@163.com WWW:Wangww2000@yahoo.com ZL: Liang2046@163.com ZLY: bytx1974@163.com ZJQ: jiquanzhao2010@163.com ... 2009[1] In China around 23,000 new patients with RCC are diagnosed each year, and the incidence is increasing rapidly due to the aging population [2] Approximately 60% of patients have clinically localized ... and analyzed the data statistically WWW performed immunocytochemical staining ZL performed immunohistochemical staining ZLY and ZJQ performed immunofluorescence microscopy and image analysis DCH...
- 28
- 266
- 0
Báo cáo y học: "Production, purification and characterization of polyclonal antibody against the truncated gK of the duck enteritis virus" pps
... the rabbit anti-tgK polyclonal antibody Lane1 represented that the polyclonal antibody was cursorily extracted by saturated ammonium sulfate; Lane stood for the purified polyclonal antibody by ... transferred onto polyvinylidene fluoride (PVDF) membrane at 120 V for 1.5 h in a BioRad mini Trans-Blot electrophoretic transfer cell (BioRad, Shanghai, China) for western blot analysis The blotted ... chromatography The heavy chain and light chain were approximately 55 KDa and 22 KDa, respectively B Western blot assay Lane 1, Western blotting analysis showed that a specific band was recognized by rabbit...
- 7
- 270
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008