... studies of our group and others, the transcriptional regulatory sequences of the H19 and IGF2 genes emerged as candidates for cancer targetedtherapy H19 and IGF2 (the human P3 and P4 promoters) ... were done in triplicates and the results expressed as mean and standard error In vitro targetedtherapy The cells were cotransfected with μg of the LucSV40 control vector and with the indicated ... obtained for the H19 and IGF2-P4 transcript were purified from the gel by the DNA and Gel Band Purification Kit (Amersham), and used as templates for the PCR-based incorporation of T7 and Sp6 RNA polymerase...
... Hepatocellular Carcinoma Kelly M McMasters Jean-Nicolas Vauthey Editors Hepatocellular Carcinoma TargetedTherapyand Multidisciplinary Care 13 Editors Kelly M McMasters, MD, PhD Department of Surgery ... Media (www.springer.com) Foreword It is a great pleasure and an honor to write the preface for this outstanding book dedicated to the therapy of hepatocellular carcinoma (HCC) This tumor, which ... liver is a massive and complex organ requiring an excellent knowledge of its anatomy and physiology, with an exquisite comprehension of its impact on cardiovascular, pulmonary, and renal function...
... Pritesh Mutha, and Sanjay Gupta 319 21 Cytotoxic Chemotherapy and Endocrine Therapy for Hepatocellular Carcinoma Daniel Palmer and Philip J Johnson 337 22 Targeted Therapies ... Albany, and Ghassan K Abou-Alfa 355 23 The Future: Combination SystemicTherapy for Hepatocellular Carcinoma Ahmed O Kaseb and Melanie B Thomas 369 24 Follow-Up and Salvage Therapy ... India, and Africa Genotypes B and C are common in China, Japan, and Southeast Asia Genotype D is common in Mediterranean areas and in the Middle East [16] Genotypes E–G are common in Central and...
... Arachidonic acid Prostaglandin COX-2 Fig 1.2 Steps in hepatocarcinogenesis, modified from Xu et al [90] and Bensinger and Tontonoz [91] On the other hand, the association between obesity and HCC is hammered ... (mycotoxins) produced by Aspergillus flavus and A parasiticus There are four AF compounds: B1 , B2 , G1 , and G2 [55] The most common and most toxic AF is AFB1 , and the most important target organ is ... by Reddy and colleagues [97] from Mayo Clinic who assessed the association between hypothyroidism and HCC among 54 HCC patients of unknown etiology and 116 HCC patients related to HCV and alcohol...
... including DNA level, RNA level and protein level, with regard to chromosomal imbalance and genetic instability, epigenetic alteration, gene expression and gene regulation and translation [1] Whatever ... proteins, genes and miRNAs in biological pathways Furthermore, significant and complex cross talks among the different pathways exist and are involved in different aspects of HCC development and progression ... overexpression, ligand-independent signalling through structural alteration of receptors and alterations in components of the downstream cytoplasmic circuitry that receives and processes the signals...
... clinicopathological factors such as proliferation, vascular invasion and tumour multiplicity and was reported to associate with not only invasion and metastasis of HCC but also postoperative recurrence [29] ... telomere shortening influences stem cell function, aging and carcinogenesis These mice exhibit an impaired maintenance and function of adult stem cells and reduced regenerative reserve in response to ... regeneration and sometimes in neoplastic transformation [59, 60] This relationship to fat metabolism appears to be important both in NAFLD-related cancer and in HCV, where steatosis, steatohepatitis and...
... subtypes, frequently found simultaneously, are trabecular, pseudoglandular (acinar), compact, and scirrhous The trabecular and acinar patterns are commonly observed in well to moderately differentiated ... of all cases) These tumors occur at a young age and are not associated with common risk factors such as chronic hepatitis and cirrhosis [35–38] and thus are frequently amenable to surgical resection ... inclusions, and fibrinogen containing pale bodies, presenting as pale ground-glass cytoplasmic inclusions [24, 36, 41] Combined Hepatocellular Carcinoma and Cholangiocarcinoma Combined HCC and cholangiocarcinomas...
... demonstrated in up to 96% of HCC [65] Polyclonal CEA and CD10 Both polyclonal CEA and CD10 (neprilysin) antibodies have cross-reactivity with glycoprotein I and exhibit a canalicular distribution pattern ... be negative, and occasional metastatic adenocarcinomas have been reported to be immunoreactive [76] Cytokeratin Low molecular weight keratins, including CAM 5.2 and cytokeratins and 18, usually ... adenomatous hyperplasia, and HCC [62, 77, 78] Diffuse CD34 sinusoidal reactivity would support a diagnosis of HCC [79] and can help differentiate dysplastic nodules and early HCCs from macroregenerative...
... and patients with chronic HCV infection who have cirrhosis stand a very high risk of developing HCC [19] In Japan, HCV infection spread throughout the country mainly in the 1950s and 1960s, and ... their design and in the characteristics of the targeted patients in terms of, for example, disease etiology and severity of background liver diseases Moreover, the reported specificity and sensitivity ... noninvasive Although both the ribs and the air in the lungs and gastrointestinal tract surround the liver and potentially hinder imaging, newer ultrasound devices and techniques have improved hepatic...
... systems may serve to guide choice of therapy, aid in patient counseling, allow comparisons of the end results of therapy, and facilitate patient selection and randomization for research protocols ... US, spiral CT, and MRI and correlation with pathologic examination of explanted liver J Comput Assist Tomogr 25:327–336 50 Miller WJ, Federle MP, Campbell WL (1991) Diagnosis and staging of hepatocellular ... P, Albertini A, Decarli A et al (2002) Alcohol and hepatocellular carcinoma: the effect of lifetime intake and hepatitis virus infections in men and women Am J Epidemiol 155:323–331 24 Kuper H,...
... discussions regarding the use of systemictherapy for HCC Systemic chemotherapy is generally ineffective against HCC, with response rates to doxorubicin of about 10%, and 20% for a combination regimen ... management of chronic cirrhosis and viral hepatitis and in implementing HCC screening protocols The hepatologist is also essential in assessing a patient’s candidacy for and optimizing a patient’s ... system (and related JIS score) may, however, have particular advantages in patients with early HCC As the therapeutic modalities available for HCC and the indications for aggressive therapy expand,...
... including liver resection and liver transplantation, percutaneous ablation therapy as represented by radiofrequency ablation (RFA) and percutaneous ethanol injection (PEI), and transcatheter arterial ... the results of liver resection and PEI when the degree of liver damage was B and the tumor was solitary and no more than cm in diameter, percutaneous ablation therapy is also recommended Although ... Study Group of Japan, and replies were 94 K Hasegawa and N Kokudo obtained from 843 (37%) of them Of those who replied, 55.4% were hepatologists and 38% were liver surgeons, and more than 70% of...
... and above a certain level and reflects it, and it is useful for explaining the complex decision-making process to patients in a way that is easy to comprehend, and for obtaining their understanding ... 96 K Hasegawa and N Kokudo Conclusion Because many effective methods are available to treat HCC and the balance between tumor status and liver function status must be taken ... 242:36–42 17 Chen MS, Li JQ, Zheng Y et al (2006) A prospective randomized trial comparing percutaneous local ablative therapyand partial hepatectomy for hepatocellular carcinoma Ann Surg 243:321–328...
... permission) alcohol injection in five, cryotherapy in two, and transarterial oily chemoembolization in two) with 63 patients who underwent nonsurgical therapyand showed that partial hepatectomy resulted ... survival of 3.4 years and 1-, 3-, and 5-year survival rates of 82, 42, and 42%, respectively Recurrent HCC Tumor recurrence represents the major drawback after curative liver resection and the most common ... hepatitis and cirrhosis (“late recurrence”) In the largest series, reresection rates have been reported between 10 and 31% and depend on the underlying liver status, pattern of recurrence, and extent...
... (TLV) is defined as the standardized FLR (sFLR) and it provides the percent of TLV remaining after resection Preoperative Therapy Transarterial Chemoembolization (TACE) and Portal Vein Embolization ... Advances in anesthetic and surgical techniques, as well as a thorough understanding of the liver anatomy and tumor biology, have contributed dramatically to the safety and effectiveness of liver ... mechanism of intrahepatic recurrence, and later to extrahepatic organs such as the lungs, bone, and adrenal glands These two forms of spread, vascular invasion and intrahepatic metastasis, are among...
... chemotherapy for a previously unresectable hepatocellular carcinoma J Hepatobiliary Pancreat Surg 13:347–350 43 Lau WY, Leung TW, Lai BS et al (2001) Preoperative systemic chemoimmunotherapy and ... faster parenchymal transection time in the group randomized to ultrasonic dissector and saline-linked cautery compared to ultrasonic dissector and bipolar cautery in living donor hepatectomy This ... complications such as bile leak and hemorrhage that needed surgical or radiologic interventions Outcome After Resection Morbidity and Mortality Improvements in patient selection and surgical technique...
... veins as landmarks to reduce the risk of major morbidity and mortality Definition of the tumor–vessels relationship is relevant for planning the type of resection, and based on that, specific and original ... volume, its stability, and the wideness of the ultrasonographic scanning window: the best probe should be small, thin in width, and short in transverse length, stable, and with a wide ultrasonographic ... the contact area between the probe and the liver and a scanning window which enlarges as it gets deeper It is also evident, how at IOUS, the portal vein (PV) and the hepatic vein (HV) have different...
... method for the surgeon to understand the liver anatomy and the relations between tumors and intrahepatic vessels This information is crucial for planning the resection and in this sense IOUS probably ... liver till the in- and outflow are proper in terms of velocity and waveform [39] Conclusions IOUS still remains the best method for staging liver involvement by the tumor, and it has been discussed ... well visualized (arrows) at IOUS, and it runs towards the tumor (T) visualized by IOUS If the target site is correct, the portal branch is ligated and divided and segmentectomy is completed under...
... patient’s age and comorbidities (e.g., diabetes) that may affect hypertrophy and perioperative outcome Thus, after all of these factors have been evaluated and the patient remains a candidate for ... increased after 24 h, tripled after days, and reached a plateau between and 14 days, whereas the regeneration rate of the cirrhotic livers was delayed and of a lesser degree Findings in clinical ... easily obtain an accurate and reproducible FLR volume that can be calculated within minutes of imaging and with a margin of error