swi snf chromatin remodeling complex involved in rna polymerase ii elongation process in drosophila melanogaster
Báo cáo khoa học: Activator-binding domains of the SWI ⁄ SNF chromatin remodeling complex characterized in vitro are required for its recruitment to promoters in vivo pot
... DABDsnf5) lacking the Snf5 activator-binding domain (SNF5 D2-327) and strain YPP247 (dark grey bars, DABDswi1) lacking the Swi1 activator-binding domain (SWI1 D329-655) The level of SWI ⁄ SNF enrichment ... min), in strain YPP310 (light grey bars, DDABDswi1 + snf5 ) lacking both activator-binding domains (SWI1 D329-655, SNF5 D2-327), strain YPP211 (white bars, DABDsnf5) lacking the Snf5 activator-binding ... SWI ⁄ SNF in relation to other possible recruitment mechanisms mediated via the nonspecific DNA binding domains in the complex, the acetyl-histone binding bromo-domain of Swi2 ⁄ Snf2 or protein...
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Báo cáo y học: " Effect of SWI/SNF chromatin remodeling complex on HIV-1 Tat activated transcription" pps
... the SWI/ SNF chromatin remodeling complex Using a number of in vitro binding as well as in vivo ChIP assays, we detected the presence of both BRG1 and acetylated Tat in the same complex Finally, ... the SWI/ SNF chromatin- remodeling complex This interaction was regulated by Tat acetylation at lysine 50 Tat recruited the SWI/ SNF complex to the LTR in vivo, leading to the activation of the integrated ... II (RNAPII) by a number of kinases, including TFIIH [17-19], hSpt5 which functions in transcription elongation as a stabilization factor of RNAPII [20], and binding of Tat directly to RNAPII [21,22]...
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Tài liệu Báo cáo khoa học: Involvement of two positively charged residues of Chlamydomonas reinhardtii glyceraldehyde-3-phosphate dehydrogenase in the assembly process of a bi-enzyme complex involved in CO2 assimilation doc
... directed against recombinant C reinhardtii CP12 (1 : 2000) or a rabbit antiserum directed against recombinant C reinhardtii GAPDH (1 : 5000) Antibody binding was revealed using alkaline phosphatase ... previously shown that proteinprotein interactions can result in information transfer, imprinting effects and can modify the regulatory properties of the enzymes involved in this complex [1620] GAPDH ... residues, Lys128 and Arg197 (C reinhardtii numbering, corresponding to Lys122 and Arg191 in spinach) that seem to protrude and could hence play a role in proteinprotein interactions (Fig 1A) Hydrophobicity...
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Báo cáo y học: " Differential patterns of intronic and exonic DNA regions with respect to RNA polymerase II occupancy, nucleosome density and H3K36me3 marking in fission yeast" pps
... Distinct chromatin patterns in intronic regions To our surprise, intronic regions showed distinct patterns with respect to the chromatin- related features The overall H3 occupancy was lower in introns ... showed distinct chromatin patterns Unexpectedly, Pol II occupancy was much higher in intronic than in exonic DNA regions, most notably in lowly expressed genes This differential marking of introns ... expression, there is decreasing H3K36me3 modification and increasing Pol II accumulation within intronic regions relative to exonic regions Pol II enrichment in intronic regions In accordance with the...
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Báo cáo y học: " Chromatin meets RNA polymerase II" pptx
... snapshot methods of protein crosslinking and DNA footprinting in Drosophila that changes in the chromatin structure at the heat-shock protein locus HSP70 are dependent on binding of heatshock factor ... controls Pol II elongation status by regulating the ability of TFIIF and NELF to engage the elongation complex He found that TFIIF alone was unable to associate with a Pol II complex containing NELF ... Pol II movement into the coding region of the gene He reported that inhibition of the activating transcription elongation factor kinase, P-TEFb, blocked the transition to elongation, suggesting...
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Báo cáo y học: "nomic mapping of RNA polymerase II reveals sites of co-transcriptional regulation in human cells" pot
... Transitions in RNA polymerase II elongation complexes at the 3' ends of genes EMBO J 2004, 23:354-364 Ahn SH, Kim M, Buratowski S: Phosphorylation of serine within the RNA polymerase II C-terminal domain ... towards terminal exons over initiating exons (Figure 4) This is probably reflecting the stalling of PolII during the coupled processes of transcription termination and 3'end processing [15] For ... domain of RNA polymerase II J Biol Chem 1996, 271:19009-19012 Komarnitsky P, Cho EJ, Buratowski S: Different phosphorylated forms of RNA polymerase II and associated mRNA processing factors during...
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THE ROLE OF THE CTD PHOSPHATASE RTR1 AND POST-TRANSLATIONAL MODIFICATIONS IN REGULATION OF RNA POLYMERASE II
... Phosphorylated serine of yeast RNA polymerase II C-terminal domain S5 Serine of yeast RNA polymerase II C-terminal domain S5-P Phosphorylated serine of yeast RNA polymerase II C-terminal domain S7 Serine of ... transcription elongation factor b PTM Post-translational Modification RNA Ribonucleic Acid RNAPII Yeast RNA polymerase II complex S2 Serine of yeast RNA polymerase II C-terminal domain S2-P Phosphorylated ... enzymes in higher eukaryotes (Vannini & Cramer, 2012) In eukaryotes, RNA polymerase II (RNAPII) is primarily responsible for transcribing DNA into messenger RNA (mRNA) as well as small nuclear RNA...
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A cullin 1 based SCF e3 ligase complex directs two distinct modes of neuronal pruning in drosophila melanogaster
... activating protein HECT homologous to E6 associated protein C-terminus Hh hedgehog IGF insulin like growth factor InR insulin receptor IPB infra-pyramidal bundles xv IPC insulin producing cell IRS insulin ... E6-associated protein C-terminus) or RING (Really Interesting New Gene) domain containing E3 ligase While HECT domain E3s are involved in the direct catalysis of the substrate, RING domain E3s serve as an ... counterpart, insulin signaling in Drosophila also regulates cell or systemic growth Disruption of insulin signaling through ablation of dilp-1, 2, and producing brain insulin producing cells reduces...
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Genetic analysis of the role of ARP2 3 complex in border cell migration in drosophila melanogaster
... al 1997) Since profilin binds to actin monomer, FH1 domains can bind the profilin-G-actin 13 Arp2/3 complex in border cell migration Lu Ruifeng 2010 complex near the barbed end of actin filaments ... (Perinuclear binding protein and substrate for protein kinase C) is a neuronal BAR domain-containing protein and regulates postsynaptic trafficking of glutamate receptors A VCA-like domain was ... FH2, including the Diaphanous autoregulatory domain (DAD), DAD interacting domain (DID), and GTPases binding domain (GBD) (Li and Higgs 2005) DAD interacts intramolecularly with DID and inhibits...
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Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx
... phosphorylation by casein kinase in modulating Antennapedia activity in Drosophila Genes Dev 11, 1327–1340 Boldyreff, B & Issinger, O.-G (1997) A-Raf kinase is a new interacting partner of protein kinase CK2 ... TGGGATTCATTTGACCA-3¢ (the gene encoding the Drosophila CK2 a subunit does not contain introns in the coding region [21]) The BamHI–HindIII digested PCR fragment was subcloned in the pQE 30 vector CK2b¢ ... Biosynthesis of casein kinase II ¨ in lymphoid cell lines Eur J Biochem 220, 521–526 12 Guerra, B., Siemer, S., Boldyreff, B., Issinger, O.-G (1999) Protein kinase CK2: evidence for a protein kinase CK2b...
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Báo cáo khoa học: DNA polymerase e associates with the elongating form of RNA polymerase II and nascent transcripts pot
... an RNA pol II holoenzyme complex that also comprised several factors involved in DNA repair and recombination, including Pol e The latter complex contained mainly hypophosphorylated RNA pol IIA, ... for staining of Pol e (antibody G1A) and all forms of RNA pol II (antibody N20) No difference in focal staining and colocalization was detected at different time points Staining RNA Pol II Ser2-P ... signal for the co-ordinated sequestration of processing factors required for mRNA capping, splicing and 3¢ end processing [4] The RNA pol II holoenzyme may also integrate mRNA transcription with...
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Báo cáo khoa học: Characterization and cDNA cloning of a clofibrate-inducible microsomal epoxide hydrolase in Drosophila melanogaster potx
... described using a cyclodiene analogue as a substrate [18] Insect hydrolases including EHs metabolize cyclodiene insecticides [19] However, EH activities in DDT-resistant D melanogaster and insecticide-resistant ... signify amino acids identical among all three proteins; dots indicate amino acids identical between two proteins The small box indicates the position of substituted amino acids within the mEH ... precisely determined in this study Similar rapid and transient activations of self-defence protein genes in insects occur when insects are infected with microorganisms [35], in which case antimicrobial...
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Báo cáo khoa học: Investigating RNA polymerase II carboxyl-terminal domain (CTD) phosphorylation ˆ Benoıt Palancade and Olivier Bensaude pptx
... progression of RNAP II along the genes in vivo (Fig 2) Interestingly, an alanine scanning analysis of the CTD in yeast RNAP II had suggested that phosphorylation on both serine and serine position in the ... These findings are consistent with an H14 staining attributed in part to initiating polymerases (productive or abortive) and an H5 staining of elongating polymerases Conclusion Determining the ... C-terminal domain of RNA polymerase II couples mRNA processing to transcription Nature 385, 357–361 87 Skaar, D.A & Greenleaf, A.L (2002) The RNA polymerase II CTD kinase CTDK-I affects pre-mRNA-3¢...
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Báo cáo Y học: Distinct parts of minichromosome maintenance protein 2 associate with histone H3/H4 and RNA polymerase II holoenzyme pptx
... protein interactions and the precise biochemical role of MCMs in regulating DNA replication remain unclear There are certain indications that MCMs might be involved in pol II transcription in higher ... encoding the indicated amino acid residues in the full length protein were cloned into pFLAG-CMV-2 All sequences contain a N-terminal FLAG tag (+) denotes that binding of the expressed protein ... blotting with the indicated antibodies The figure shows one of two independent experiments Point mutations in the MCM2(169–212) domain disrupt the binding of pol II holoenzyme in vitro and in vivo...
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Báo cáo khóa học: Three cyclin-dependent kinases preferentially phosphorylate different parts of the C-terminal domain of the large subunit of RNA polymerase II potx
... C-terminus of the RNA pol II CTD is required for transcription, splicing and 3¢ end processing EMBO J 22, 4274–4282 10 Bentley, D (2002) The mRNA assembly line: transcription and processing machines ... (2001) Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxylterminal domain independently of cyclin-dependent kinase-activating kinase J Biol Chem 276, 12317–12323 ... spreading of phosphorylation by this kinase into the C-terminal portion Thus, the CTD seems to be separated into two subdomains The C-terminal subdomain is mainly phosphorylated by the processive...
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Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx
... Gal(b1-3)GalNAc-binding lectin peanut agglutinin failed to reveal any signal in embryonic salivary glands [8], which could mean that salivary O-glycan chains are elongated, thus abrogating peanut agglutinin ... abrogating peanut agglutinin binding Furthermore, the peanut agglutinin staining in the developing nervous system documented by D’Amico and Jacobs [8] could not be confirmed in our in situ hybridization ... proteinÆlL)1 slurry The integrity and amounts of FLAG-tagged recombinant proteins were inspected by western blotting Cloning of Drosophila cDNAs Total RNA was extracted from tight-rod disintegrated...
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Báo cáo sinh học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" pptx
... lentiviral structural mRNAs [5] Whether similar mechanisms govern the fate of recombinant Pol II mRNAs of viruses replicating in the cytoplasm is unclear Instead of using cellular RNA polymerases for ... viruses have a regulatory protein (Rev) involved in shuttling the mRNA for the structural proteins from the nucleus to the cytoplasm [4] Retention of these lentiviral mRNAs in the nucleus has been attributed ... Figure of Analysis RSV-F mRNA processing Analysis of RSV-F mRNA processing A) Map of exon-intron structure and poly(A) signals of the precursor mRNA encoded by pIFwt Arrows indicate location of primers...
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Báo cáo hóa học: " Expression of RNA virus proteins by RNA polymerase II dependent expression plasmids is hindered at multiple steps" ppt
... lentiviral structural mRNAs [5] Whether similar mechanisms govern the fate of recombinant Pol II mRNAs of viruses replicating in the cytoplasm is unclear Instead of using cellular RNA polymerases for ... viruses have a regulatory protein (Rev) involved in shuttling the mRNA for the structural proteins from the nucleus to the cytoplasm [4] Retention of these lentiviral mRNAs in the nucleus has been attributed ... Figure of Analysis RSV-F mRNA processing Analysis of RSV-F mRNA processing A) Map of exon-intron structure and poly(A) signals of the precursor mRNA encoded by pIFwt Arrows indicate location of primers...
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Báo cáo khoa hoc:" Comparative influence of Odh and Adh loci on alcohol tolerance in Drosophila melanogaster" pot
... different (figure 1) In contrast, all the four strains containing the OdhF’! allele originated from the same isofemale line ( figure B) The isolation of all strains was completed in six generations ... significantly in their initial survival rates in both life stages (table I: IFL) The strains originating from isofemale line A (figure 1) had lower survival in the absence of ethanol in both the larva-to-pupa ... strains with Adh genotype (originating from three different isofemale lines) and four strains with s Adh genotype (originating from two isofemale lines) were analysed in this study (figure 1) McKenzie...
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Báo cáo khoa hoc:"Body size reaction norms in Drosophila melanogaster: temporal stability and genetic architecture in a natural population" docx
... the W/T ratio by calculating the co-ordinates of the inflexion points (table IQ, that is, the phenotype at the inflexion point (PIP) and the temperature of the inflexion point (TIP) For this character, ... explain It rules out, however, the above-mentioned possible bias of measuring the same thing twice In the future, evolvability of a trait should receive increasing attention As discussed in the Introduction, ... Statsoft Inc., Tulsa, OK, USA, 1995 [44] Tantawy A.O., Studies on natural populations of Drosophila III Morphological and genetic differences in wing length of D melanogaster and D simulans in relation...
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