surface properties of native s layer lattices from bacillaceae
Tài liệu Fertility, Family Planning, and Reproductive Health of U.S. Women: Data From the 2002 National Survey of Family Growth doc
... last weeks These two tables show similar relationships between consistency of condom use and marital status, marital status and age, and cohabiting status As expected, more consistent condom use ... more years too soon (as opposed to less seriously mistimed (Table 21)) The percentage of recent births seriously mistimed decreases from 15 percent among mothers with less than a high school diploma ... characteristics of the pregnancy are also shown, such as respondent s age at birth and wantedness of the pregnancy at time of conception The definitions of variables used in this report, as well as the...
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Reproductive contributions of Taiwan''''s foreign wives from the top five source countries pot
... census of the foreign spouses of Taiwanese citizens in 2003 The universe of the census was created by merging (1) the records of foreign spouses (not including those from China) in the data system ... probability of childlessness slightly (by 3% or less) In sum, the highest risks of lifetime childlessness resulted from (1) marrying beyond the mid-3 0s and (2) having a separated marital status The ... decades (Douglass 2006) The creation and sustainment of households at all lifecycle stages increasingly depends on short- and long-term migrations of individuals across the borders of nation-states...
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Báo cáo khoa học: Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis ppt
... Zhongshan station in Larsemann Hills, and collected Syw-1 from the soils in Kizahashihama, Skarvsnes, Soya coast DNA was extracted from the A psychrotrophicus strains KS-1, Z-23 and Syw-1 using ... fish, insects, plants and microorganisms have been reported to consist of five to 10 isoforms [12,21–25], it could be assumed that AnpAFP consists of approximately 10 isoforms Here, AnpAFP, TisAFP ... 5) consist of a mixture of AFP isoforms in A psychrotrophicus, Typhula ishikariensis and Zoarces elongatus, respectively We attempted to determine the primary sequence of the isoforms of AnpAFP,...
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Báo cáo khoa học: Enzymatic properties of the lactate dehydrogenase enzyme from Plasmodium falciparum pdf
... that not possess the extra five residues in the substrate-specificity loop Crystal structures of holo-LDHs with serine at position 163 show that the hydroxy group of the serine side-chain is hydrogen ... investigate this possibility, the activity of the enzyme was tested with alternative substrates for comparison with other well-characterized LDHs; Table shows a summary of the results There was ... respectively Initially, a series of diagnostic steady-state experiments were designed to assign the general kinetic mechanism In these enzyme assays, NADH and pyruvate were used as the substrates...
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Báo cáo khoa học: Properties of purified gut trypsin from Helicoverpa zea, adapted to proteinase inhibitors pdf
... of proteases So far, insect serine proteases could not be expressed as active enzymes in a variety of hosts tested (Escherichia coli, yeast, insect cells; unpublished data), so no definite assignment ... energy-consuming change in gene expression of protease genes Synthesis of proteases is an important metabolic activity of the gut cells, as protease mRNAs make up 20% of gut cDNAs [9] One suggested ... HzTrypsin -S, is synthesized, which is highly insensitive to all plant inhibitors tested It is puzzling why these insects not constitutively express the inhibitor-insensitive trypsin genes, but instead...
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Báo cáo khoa học: Altering the surface properties of baculovirus Autographa californica NPV by insertional mutagenesis of the envelope protein gp64 ppt
... produced five successive virus stocks These stocks were examined for virus titre and surface expression of the ELDKWA peptide on infected cells using FACS (Fig 3) For FACS, triplicate infections of 106 ... peptides designed for specific functions, e.g virus targeting to selected tissues, extension of host range or enhancement of transduction efficiency of nonpermissive cells are envisaged as future aims ... experiments have shown that this strategy leads to a drastic increase in avidity of proteins as compared to previously described methods (unpublished results) ACKNOWLEDGEMENTS The authors thank G Blissard...
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Báo cáo khoa học: Mechanistic aspects and redox properties of hyperthermophilic L-proline dehydrogenase from Pyrococcus furiosus related to dimethylglycine dehydrogenase⁄oxidase potx
... 4Fe) 4S ferredoxin This article represents the first detailed report of the kinetic and spectroscopic properties of this novel type of PRODH Results Analysis of a and b subunit sequences Sequence ... Manchester, UK), using sinapinic acid as matrix Electrospray MS was performed using a Waters Micromass LCT TOF mass spectrometer (Waters Ltd., Elstree, UK) L-proline dehydrogenase from P furiosus ... Discussion The mechanism of substrate oxidation by flavoprotein amine dehydrogenase ⁄ oxidases remains contentious In recent years, however, substrate oxidation by members of this enzyme class...
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Báo cáo khoa học: Biomechanical properties of native basement membranes docx
... the mean ± SD surfaces, such as glass or plastic, allowing reliable thickness measurements It is also of note that the ILM isolation method uses firmly mounted retina as a source; thus, the preparation ... USA) The filter ⁄ retinal sandwiches were placed, vitreous surface down, on poly lysine-coated (Sigma, St Louis, MO, USA) glass slides (Superfrost ⁄ plus, Fisher Scientific, Pittsburgh, PA, USA) ... Increases in both the membrane thickness and stiffness (Young s modulus) would enhance BM strength to resist stress induced during the growth of the eye The change in stiffness is biologically useful...
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Báo cáo hóa học: " Surface properties of glass micropipettes and their effect on biological studies" pot
... with each set of parameters and their tip sizes were measured by SEM Figure is a summary of the statistics of the experiments A few sudden variations in tip sizes are mainly because of the nonhomogeneities ... carriage system as the glass softens By increasing the velocity, both the tip size and the surface roughness decrease The velocity has the most significant effect on the tip size and the surface ... increasing the tip size, surface roughness also increases As an example, the surface properties of two pipettes with considerably different tip sizes are shown in Table The bigger pipette has higher...
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Báo cáo hóa học: "BASIC PROPERTIES OF SOBOLEV’S SPACES ON TIME SCALES" pdf
... (2.10) As we know from general theory of Sobolev s spaces, another important class of functions is just the absolutely continuous functions Definition 2.9 A function f : J → R is said to be absolutely ... ≡c Δ-a.e on J o (3.11) Basic properties of Sobolev s spaces on time scales Proof The necessary condition is consequence of the fundamental theorem of Calculus Conversely, fix u ∈ Cc (J o ) arbitrary; ... (4.1) 12 Basic properties of Sobolev s spaces on time scales n,p It is easy to prove the following characterization of the set WΔ (J) p n,p ¯ Proposition 4.2 Suppose that u : J → R is such that...
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Báo cáo lâm nghiệp: "Comparison of physical and mechanical properties of tension and opposite wood from ten tropical rainforest trees from different species" pptx
... positions 4, and there was never high level of growth strains, as it is usual for opposite wood Specimens from these positions were used for calculation of mean values of opposite wood properties 3.2 ... Figure Size of samples used for mechanical and physical properties CSCP: Crushing strength in compression parallel to the grain CSSB: Crushing strength in static bending 2.7 Crushing strength ... in softwoods, tension wood in hardwoods seems to be much more diverse in its technological properties This point reflects more diverse strategies in fibre differentiation This means also that tension...
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Báo cáo y học: "Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus" ppsx
... cytokines Polarity IFN-γ TNF-α TNF-β IL-1α IL-1β IL-6 iNOS β-actin Sequence Product size sense antisense sense antisense sense antisense sense antisense sense antisense sense antisense sense antisense ... test All data were reported as means ± SD A P value of less than 0.05 was considered significant Results Analysis of expression of the MuLV gene and protein in the brains of ICR, SAMR1 and SAMP8 ... enous retroviruses have been described in other mammals and in birds; it was suggested that some types of cancers are induced by endogenous retroviruses in these species [27] The SAMP8 mouse strain...
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Properties of pseudoscalar flavor singlet mesons from lattice QCD
... extrapolations, leading to a rather complete assessment of the systematic uncertainties in our study Through special techniques we will achieve precise values for the masses of the η and η ′ mesons as well ... values of the lattice spacings While one of the main benefits of Wilson twisted mass fermions is its property of so-called automatic O (a) improvement, this does not exclude the possibility of ... observables) of our studies In addition, we include a summary of the statistical methods used for the data analysis in this work In chapter we present the results and details of our analysis This covers...
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Báo cáo khoa học: Purification and properties of a new S-adenosyl-Lmethionine:flavonoid 4¢-O-methyltransferase from carnation (Dianthus caryophyllus L.) pot
... spite of its high selectivity in the catalyzed methylation, this enzyme possesses some characteristics that are commonly shared by other previously described OMTs In fact, F 4¢-OMT consists of ... the same amounts of both Fod-inoculated and Fod-uninoculated tissues, with the aim of assessing if the F 4¢-OMT activity could be associated, to some extent, to the tissue s defense response HPLC ... chromatographic steps needed to be accomplished as rapidly as possible, as the enzyme proved to quickly loose its activity in the course of time: a storage period of weeks at )20 °C caused a 50% loss of...
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Báo cáo khoa học: Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle, Trachemys scripta elegans pdf
... lower specific activities of GSTs in liver from anoxic turtles (using either CDNB or cumeme hydroperoxide as substrates) suggest a possible specific suppression of GST activity during anaerobiosis, ... suggestive of l class which typically shows only minor activity towards cumene hydroperoxide The classification of GSTs also depends on their responses to substrates and inhibitors Like all GSTs, ... The native molecular mass of some yeast (Y lipolytica) GSTs, however, is 110 kDa [32] Thus GSTs can vary widely in their subunit size The larger molecular mass of turtle GSTs may arise as a result...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... the presence of USP increases ligand-binding affinity [12–15] The diversity of ligand-responsive characteristics seen among ecdysteroid receptors from various insect species suggests a basis for ... insecticides acting as disruptors of normal ecdysteroid action This possibility increases the importance of evaluating the heterologous cell culture assay as a valid tool for the assessment of ecdysteroid ... receptors from several species, this work focuses on a direct and thorough comparison of several attributes associated with well-described ecdysteroid receptors from two insect species for which...
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Tài liệu Báo cáo khoa học: Role of hydroxyl group and R/S configuration of isostere in binding properties of HIV-1 protease inhibitors docx
... The aspartate that does not bind to the isostere NH seems to make weak hydrogen bonds to both CH2 groups of the isostere The changes of torsion angles in the isostere also resulted in shifts of ... Review of interactions of native HIV-1 protease with inhibitors OE, RE, SE analysed residue by residue Comparison of protease residues which are in contact with individual side chains of inhibitors ... Comparison of hydroden bonds (2.4–3.6 A) of inhibitors OE, RE and SE towards their HIV-1 proteases Stated numbers are distances ˚ between inhibitor and protease atoms in A Reside of Atoms of Inhibitor...
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Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... expression strain supplying tRNAs for these codons, and the E coli Rosetta strain was tested as such a host As an alternative, another expression vector was constructed, p24-glucose dehydrogenase, which ... these enzymes in P torridus The results from testing the substrate specificity of the purified recombinant GdhA indicate a relatively strict range of substrates Nevertheless, the enzyme was considerably ... as in the previous step The level of purification of the heterologously expressed protein at each step was monitored by measuring the specific glucose dehydrogenase activity and assessed by SDS...
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Tài liệu Báo cáo khoa học: Enzymatic properties of wild-type and active site mutants of chitinase A from Vibrio carchariae, as revealed by HPLC-MS pptx
... monomer of Mr 62 700 Analysis of chitin hydrolysis by using the viscosity assay and HPLC-ESI MS suggested that the newly isolated chitinase acts as an endochitinase [25] We also reported isolation of ... masses from monomer to hexamer Identification of b and a anomers was assessed from previous experiments with equivalent reverse-phase HPLC system and 1H NMR [14] Transglycosylation of chitinase ... composed of an array of subsites, probably six GlcNAc subsites This corresponds to the structural data obtained for Serratia marcescens ChiA and the hevamine chitinase [12,15], in which a substrate-binding...
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Tài liệu Báo cáo Y học: Antibacterial and antifungal properties of a-helical, cationic peptides in the venom of scorpions from southern Africa pptx
... to molecular mass by the Maxent software (Micromass, UK) The masses of the peptides resulting from the tryptic digestion were determined by MALDI-TOF MS on a VG Tofspec (Micromass, UK), equipped ... presence of the peptides was studied For this purpose an assay based on the uptake of SYTOX green was used as described by Thevissen Ó FEBS 2002 4806 L Moerman et al (Eur J Biochem 269) Table Synergism ... suppresses growth of E coli at concentrations when very few hemolysis occurs S marcescens is relatively resistant to the action of the cationic peptides (Table 1) This has also been observed...
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