... seawater (Mg concentration one-tenth of control seawater), control seawater, low-Ca2? seawater (Ca concentration one-tenth of control seawater), and back to control seawater Fish were maintained ... produced inthe intestine as a result of seawater adaptation, or hyposmoregulation Evacuation of Ca precipitates is typically observed in fasting Japanese eel acclimated to seawater The Ca precipitates ... seawater-acclimated eels were placed ina 60-l glass tank containing control artificial seawater and maintained for days Then, the environmental water was changed sequentially to low-Mg2? seawater...
... Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like immunoreactivity inthe blue crab: Tissue distribution, variations inthe molt cycle, and partial characterization ... bromine Comp Biochem Physiol 11 9A: 941-950 Graf F, M Fouchereau-Peron, A Van-Wormhoudt and J.C Meyran 1989 Variations of calcitoninlike immunoreactivity inthe crustacean Orchestia cavimana during ... R.P., F.D Parado-Estepa, E.G de Jesus and J.M Ladja 1987 Osmotic and chloride regulation inthe hemolymph ofthe tiger prawn Penaeus monodon during molting in various salinities Marine Biology...
... Journal of Physiology 363:377-401 Cameron J.N and P Thomas 1992 Calcitonin-like immunoreactivity inthe blue crab: Tissue distribution, variations inthe molt cycle, and partial characterization ... bromine Comp Biochem Physiol 11 9A: 941-950 Graf F, M Fouchereau-Peron, A Van-Wormhoudt and J.C Meyran 1989 Variations of calcitoninlike immunoreactivity inthe crustacean Orchestia cavimana during ... R.P., F.D Parado-Estepa, E.G de Jesus and J.M Ladja 1987 Osmotic and chloride regulation inthe hemolymph ofthe tiger prawn Penaeus monodon during molting in various salinities Marine Biology...
... the appearance of an activity peak of DNA polymerases was consistent with the minimum inthe inhibitory activity of PMLA, an additional periodic variation inthe intrinsic activities ofthe endogenous ... microplasmodia (j) The control inthe absence ofthe nuclear extract containing an equivalent ofthe ingredients inthe nuclear extraction buffer The arrow refers to the equivalence ofthe added DNA polymerase ... extract (d) The activity was measured as a function of added amounts of DNA polymerase ainthe standard DNA polymerase assay that contained the extract of · 106 nuclei isolated from microplasmodia...
... beginning in 1996, L vannamei was introduced into Asia ona commercial scale This started in Mainland China and Taiwan Province of China and subsequently spread to the Philippines, Indonesia, ... vannamei spawn inthe ocean, releasing their eggs into the water The eggs hatch into a non-feeding nauplius larva, which lasts about two days, before molting into a zoea stage (4-5 days), a mysis ... Teichert-Coddington, and D., Rouse, D.B., 2002 Acclimation of Litopenaeus vannamei postlarvae to low salinity: influence of age, salinity endpoint, and rate of salinity reduction Journal ofthe World Aquaculture...
... MgATP inthe dimer formation ofa H4-H5 loop protein of Na+/K+ ATPase [55] In conclusion, neither method revealed any indication ofa conformational change ofthe N-domain upon ligand binding inthe ... eventually induce its bending towards the P-domain and thereby explain the amino acid labeling ofthe P-domain found with 8-N3-TNP-ADP [25] The same consideration may apply to the labeling of P668 ... respecting the N- and C-terminal parts ofthe P-domain [20], shows F548 as part ofthe ATP site (Fig 3A) , while ina model based onthe crystal structure ofthe N-domain of Na+/K+ ATPase and not including...
... against Na/K-ATPase inactivation was not due to the removal of fructose by binding to a- crystallin, BSA was resealed (in the same manner and concentration as that of a- crystallin) and incubated with ... 20 kDa) The effects of ouabain, a- crystallin and sugars onthe Rb uptake ofthe ghost cells are shown in Fig Ouabain (0.1 mM), a specific inhibitor of Na/K-ATPase, caused a 35% decrease inthe ... cataract and correlate with increasing lens colour and with cortical opacification [31] The change in monovalent cation concentrations may in part be attributed to decreased efficiency ofthe Na/K-ATPase...
... helices, and a separation inthe contact surface of membrane and protein due to the thickening and shrinkage of lipid bilayer For the last case, a quantitative estimation ofthe thickness and cross-sectional ... mM NADH, lgámL)1 of pyruvate kinase, and 2.5 lgámL)1 LDH ina total volume of mL The reaction was initiated by an addition of 50 ng of Na+/K+-ATPase Na+-dependent ATPase activity was also measured ... palmitic acid and 26.4% of oleic acid However, the shrinkage ofthe cross-sectional area is partly caused by the phase transition of lipids and is accumulative inthe lateral direction, and lastly, the...
... Stimulate cAMP generation Theophylline Degrades cAMP- leading to increased intracellular accumulation of cAMP Monensin Increases Na+ concentration Insulin/IGF-1 Increases Na+ affinity of NKA Possible ... increase affinity of NKA forNa+ β2 agonists (Salbutamol, Isoproterenol) Stimulate cAMP generation Calcitonins Stimulate cAMP generation Amylin Stimulate cAMP generation Calcitonin gene related ... program on NKA isoform abundance in human are unknown Therefore Chapter investigated whether 12 weeks of high-intensity interval training (HIT) in aged individuals induced adaptations in NKA isoforms...
... other hand, increasing the Na+ concentration had no effect onthe ouabain-insensitivity of pNPPase activity The concentration giving halfmaximal activation (K0.5) of pNPPase for K+ was mm, and the ... Na+ binding was very low inthe absence of oligomycin, which increased the affinity of Na+ ⁄ K+-ATPase for Na+ [14] The resultant increase in Na+ binding was regarded as Na+ occlusion Inthe absence ... The combination of 10 mm NaCl and 0.1 mm ATP maximally activated pNPPase Inthe presence of ATP and KCl, increasing the NaCl concentration induced convex-shaped activation curves of pNPPase (Fig...
... sites and 110 was maximal inthe G2 phase, in contrast, phosphorylation at the other sites (7, 32, 54) was maximal inthe M phase Phosphorylation of MCM4 at sites and 32 decreased during anaphase ... antibodies Staining with P-3 antibodies, anti-BrdU sera and DAPI, and a cell image to determine area are shown These are original data for quantification in Fig 5A This material is available from ... re-replication of DNA in eukaryotic cells including human cells [7] Targets ofthe kinase inthe regulation of DNA replication include ORC2, Cdc6 and Mcm proteins in Saccharomyces cerevisiae [8], and...
... signal-transduction by ouabain Fig Ouabain a nities to rat Na+/K+-ATPase isoforms and actual ouabain or OLF concentrations The figure summarizes the huge variation in ouabain a nities of Na+/K+-ATPase ... Cellular compartments with larger changes in intracellular Na+ and Ca2+ may thus be the result ofa ouabain interaction with subpopulations of Na+/K+ATPase [27] An overall increase in intracellular ... isoforms, ofwhichthe latter may have a sublocalization critical for the observations looking at ouabain as a signal-transducer Experiments pointing to a pivotal role of ouabain in signal-transduction...
... and [Ca2+]i that may occur These activated pathways, the resulting increase in ROS, and the concomitant increase in [Ca2+]i lead to activations of NF-jB and AP-1, transcriptional regulation of ... protein–protein interactions is a common property of Na+/K+-ATPase in most, if not all, cells Using partially inhibitory concentrations of ouabain, activation of parts or all ofthe pathways that begin ... ofthe diseased heart as stated in textbooks, but rather a part ofthe continuum ofthe derangements leading to advanced heart failure Because ofthe latter, at the time there was extensive ongoing...
... linear regressions ofthe original data on rectangular coordinates; case D was a linear regression ofthe logarithm ofthe original data, so that the fit to an exponential case was tested Case ... stand against the board and their height would be measured by drawing a line and dating the line At the left is the full board and at the right is a close-up ofa portion ofthe board at a constant ... grandchildren at random occasions, usually family gatherings When measuring time occurs the grandchildren stand against the board, a line is drawn at the top of their head, and the date ofthe line is...
... effector caspase and one of its substrates, poly(ADP-ribose) polymerase (PARP), by western blotting (Fig 5A) Cleavage of caspase 3, indicating activation as well as cleavage of PARP, as soon as h ... fusion variants, as well as EGFP fusion variants ofthe truncated VP3 (containing 103 amino acids ofthe C-terminus) We followed minor protein localization in mouse cells, cell death and the ... localization ofthe wt minor proteins and their fusion variants Distribution ofthe fused minor capsid proteins was followed through the analysis of confocal microscopy images of cells stained...
... TCGAGAATCTAGT TCA TAC AGC TAG ATA ACC AAA GA TAGTA TCA TAC AGC TAG ATA ACC AAA GA TAGTA TCA TAC AGC TAG ATA ACC AAA GAT CTAGA TCT TTG GTT ATC TAG CTG TAT GA TACTA TCT TTG GTT ATC TAG CTG TAT GA TACTA TCT ... GTT ATC TAG CTG TAT GA ACTAGATTC TCGAGAATC TAG TAC TTT CGG TTA TCT AGC TTT A TAGTA ACT TTC GGT TAT CTA GCT TTA TAGTA ACT TTC GGT TAT CTA GCT TTAT CTA GAT AAA GCT AGA TAA TTG AAA GT TACTA TAA AGC ... CCT AGA GTG CAA AAG CAC TTT Primer applications ATA CCC CTG TTA GCC GGC CAA ACA CCA GCG ACC AGA TAT GAT GTC TTC GAT TAG ACT GTG GAA GC GAG TTC ATA TCT GAA CGA GTG GAC tranfection Transient transfections...
... C, and enabling a conformational change in path A, thereby increasing the rate of both cycles In reaction path A, binding of Mg2+ to subunit induces a conformational change from 12 to 21 Inorganic ... of metal ion activation, and the dependence of APase activity onthe metal ion concentration In order to clarify the mode of APase activation by Zn2+, and to establish the appropriate Zn2+ ion ... substrate concentrations, could be enhanced inthe same way Both activation of APase with Mg2+ and kinetic data indicate that Mg2+ enhances the reaction rate in uencing allosteric Ó FEBS 2003 Catalytic...
... sequence is involved in interactions associated with the formation ofthe aggregate At mildly acidic pH, the fragment SKA1 in isolation can adopt a conformation rich in b sheet, containing a hydrophobic ... transitions of intact SK by consecutive heating ofthe same sample inthe calorimeter The sample concentrations and pH values are indicated inthe panels First heatings ofthe sample are represented ... equations using the following set of parameters: a linear heat capacity function for the native state, Cp(N); the enthalpies and the heat capacities ofthe unfolded state, DHU and DCpU, and of the...
... widespread lateral gene transfer among all organisms, and one combination of such transfers gave rise to the eukaryotic lineage, which some time later obtained mitochondria (the Ôgenetic annealingÕ ... cytosolic aconitase, at least in animals, is that of an ironresponsive element-binding protein (IRE-BP), which binds to mRNA of ferritin and the transferrin receptor and thus participates in regulating ... plants and animals is closely related to the eubacterial aconitase homologues termed here aconitase AThe sequences for eubacterial aconitase B proteins fall into a separate gene cluster and are...