substituted phenol derivatives on pt electrode from aqueous acidic solution kinetics mechanism electrochemical studies and characterization of the polymer obtained
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... consumption was subtracted from the consumption recorded after addition of mM phenol The effects of PHI and PHR concentrations on the overall PH activity were evaluated by systematic variation ... potential on addition of PHI (G Gilardi, Dept of Biological Sciences, Imperial College of Science, Technology and Medicine, London, UK, personal communication) On the other side, on the basis of X-ray ... reaching thermal equilibrium As shown in the inset, the progressive decrease of the molecular ellipticity at k ¼ 200 nm (the absorption region of the peptide bond) reflects the occurrence of a transition...
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Báo cáo khoa học " NMR characterization of the polysaccharidic fraction from Lentinula edodes grown on olive mill waste waters " pdf
... points on the b-(1 fi 6)-D -glucopyranosyl residues (Scheme 1) The integral of the anomeric H resonances of the a and a0 residues of the backbone compared with the integral of the anomeric H resonances ... polysaccharidic fractions were analyzed by gas chromatography (GC) and NMR spectroscopy, and the results were the same; therefore, only the analysis of the fraction extracted from the mycelium grown on OMWW ... Structural elucidation of compound A Figure H–1 H TOCSY map of the polysaccharidic fraction in 0.5 M NaOD aqueous (D2 O) solution at 300 K The H spectrum of the sample with the corresponding assignment...
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Báo cáo khoa học: Expression and characterization of the protein Rv1399c from Mycobacterium tuberculosis A novel carboxyl esterase structurally related to the HSL family docx
... 271) characterization, the Lip term could be confused to refer to a NLH, and the original accession number should be used to avoid confusion Here we report the cloning, expression and refolding of ... Results and Discussion Cloning and expression of Rv1399c The Rv1399c gene product encodes a 318 amino acid protein with a molecular mass of 31.7 kDa and a calculated pI of 4.53 and belongs to the ... [12] Among the 96 refolding conditions tested, 14 of them yielded soluble Rv1399c based on the turbidity measurement at 340 nm The choice of the final refolding buffer was guided by the optimum...
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Báo cáo khoa học: Expression and characterization of the biofilm-related and carnosine-hydrolyzing aminoacylhistidine dipeptidase from Vibrio alginolyticus pot
... examined the V alginolyticus PepD protein In the present study, we present the cloning and expression of the V alginolyticus pepD gene, the purification and biochemical characterization of the produced ... plasma-MS and X-ray atomic absorption spectrum determination These analyses demonstrated that PepD contains zinc as the divalent metal ion We then investigated the effect of metal ion substitutions on ... zinc ions and close to the carboxylate group of the catalytic Glu149; (b) upon substrate binding, the water molecule will be positioned between both zinc ions and the carbonyl carbon of the scissile...
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Báo cáo khoa học: Molecular cloning and characterization of the crustacean hyperglycemic hormone cDNA from Litopenaeus schmitti Functional analysis by double-stranded RNA interference technique pot
... by RT-PCR of a defined band of 260 bp in the saline treated group only These results suggest the possible complete degradation of the CHH transcript because of dsRNA gene silencing mechanism RNA ... of dsRNA, the region of homology between the dsRNA and the target, as well as other lesser know mechanisms [29] In recent investigations it was demonstrated that the length and dose of dsRNA determine ... N-terminal and C-terminal regions of the mature peptide To obtain the CHH cDNA, we used 10 lg of total eyestalk RNA from adult shrimp by RT-PCR assays The quality of synthesized cDNA was confirmed...
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Báo cáo khoa học " Optimisation of extraction procedure for black fungus polysaccharides and effect of the polysaccharides on blood lipid and myocardium antioxidant enzymes activities " pdf
... Vitamin E consumption and the risk of coronary heart disease in men New England Journal of Medicine, 328, 1450 Salisova, M., Toma, S., & Mason, T J (1997) Comparison of conventional and ultrasonically ... and C-6 of -d-glucose Based on the data available in the literature, it was possible to identify that the resonances in the region of 75–95 ppm were attributed to the anomeric carbon atoms of ... Application of fractional factorial experimental and Box–Behnken designs for optimization of single-drop microextraction of 2,4,6-trichloroanisole and 2,4,6-tribromoanisole from wine samples Journal of...
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báo cáo khoa học: "Triple malignancy in a single patient including a cervical carcinoma, a basal cell carcinoma of the skin and a neuroendocrine carcinoma from an unknown primary site: A case report and review of the literature" ppt
... addition, a skin inspection found a cm solitary nodule on the dorsum of the nose: the lesion bled spontaneously at the examination and biopsy confirmed the diagnosis of basal cell carcinoma of the ... involved in the analysis of the data and the literature research, and he also wrote the manuscript SB helped with the patient management and revision of the manuscript TM helped with the literature ... [5] In the case that we present, the histological type of the skin lesion as a basal cell carcinoma and the long survival after the local control of both tumors might exclude the hypothesis of skin...
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Báo cáo khoa học: "Expression, purification and characterization of the Lily symptomless virus coat protein from Lanzhou Isolate" doc
... participated in the sample preparations YW participated in the design of the study LA conceived of the study, and participated in its design and coordination and helped to draft the manuscript All authors ... results MALDI-ToF MS, Database searching for His-CP His-CP of LSV containing band was cut from the gel and destained overnight with a solution of 50 mM ammonium bicarbonate, 40% ethanol The protein ... 13-amino acid extension in C-terminal MALDI-ToF MS and phylogenetic analysis The MALDI-ToF mass spectrum of tryptic digest of the gel band of His-CP was shown in Fig.2a The achieved peptide masses were...
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Báo cáo khoa học: "Expression and characterization of the UL31 protein from duck enteritis virus" pot
... gene and strategy for construction of the Figure expression plasmid pET 32-UL31 A Schematic representation UL regions of the DEV genome containing the UL31 gene and strategy for construction of the ... However, there is no report on the identification and characterization of the UL31 gene product of DEV In the present study, the UL31 gene was amplified from the genome of DEV and successfully expressed ... Expression and purification of recombinant UL31 In the present study, DNA sequence encoding the UL31 gene was amplified from the genome of DEV (Fig 3B), and cloned into the fusion expression vector...
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Báo cáo khoa học: Synthesis and characterization of Pi4, a scorpion toxin from Pandinus imperator that acts on K+ channels doc
... binding of [125I]apamin without any other ligand, and B is the binding in the presence of the indicated concentrations of competitor Abscissa axis is the logarithm of the molar concentration of competitor ... positioning of key functional residues that participate in the interaction with the ion channel pore protein These data strengthen the idea of a multipoint interaction between scorpion toxins and their ... cluster of aromatic residues consisting of Trp344, from one of the four Kv1.2 a-subunits, and Trp345 and Tyr355 of an adjacent a-subunit The phenol ring of Tyr35 additionally forms an hydrogen bond...
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Báo cáo hóa học: " Synthesis and Characterization of ZnO Nanorods and Nanodisks from Zinc Chloride Aqueous Solution" pptx
... Results and Discussion Figure shows the morphologies of the samples prepared in aqueous solutions of equimolar of ZnCl2–HMT mixture with and without 50 vol.% EG additive The initial concentrations of ... crystal On the other hand, the samples b and d in Fig showed quite different spectra to those of Fig 3a, c A strong band due to deformation vibration of H2O molecules at 1,630 cm-1, an absorption band ... conclusions might be drawn: The morphology and crystalline phase of the product by the heat treatment of ZnCl2–HMT aqueous solution with and without EG changed greatly depending on the concentrations...
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Executive summary PHD thesis in history the policy and guideline of the communist party of vietnam on education and training cooperation with ASEAN countries from 1995 to 2010
... including the promotion of international cooperation on education and training In the continued implementation of the 1998 Law on Education and the improvement of the quality and efficiency of international ... countries on education and training Along with the international economic integration process and the accession to the AFTA and the WTO, regional and international cooperation on education and training ... from 2002 to 2005 and further onto 2010 The Central Committee agreed to continue the implementation of the Second resolution of the 8th Central Committee term, the direction for educational and...
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RESEARCH ON PHLOROTANNIN COLLECTION FROM BROWN ALGAE (SARGASSUM SERRATUM) IN NHA TRANG AND ASSAY APPLY TO ANTIOXIDANT BEVERAGE
... evaluation and determination of polyphenol component and character of their structure 3.4.2 Evaluation of phlorotannin purity The purity of fractions collected from the process of solvent fraction and ... natures, and a relation between the quantity and hyppothesis verification in the thesis 3 The new contribution to the science - This is the first time the comprehensive research on phlorotannin of ... until the humidity of 19%, storaged in the room temperature and used for the research of phlorotannin collection 3.2 The scope of research: contain parts 1) Extraction and optimization study on...
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Tài liệu Cleaner Water in China? The Implications of the Amendments to China’s Law on the Prevention and Control of Water Pollution pptx
... national standards for environmental quality” and “national standards for the discharge of pollutants” as well as a monitoring system.32 B The Law on Prevention and Control of Water Pollution and ... protection.35 In addition to the LPCWP, polluting industries are subject to a variety of rules and regulations Nationally, these include the Implementing Rules on the Law on the Prevention and Control ... standards for items not covered by the WEQ standards and must report their standards to the State Council.80 Using the WEQ standards and the country’s economic and technological conditions, the...
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Tài liệu Báo cáo khoa học: Investigation and prediction of the severity of p53 mutants using parameters from structural calculations pptx
... variable alone, we can make reasonably accurate predictions on 35% of the severe mutations and on 20% of the nonsevere mutations Even in the most difficult case, an energy value of 0.225, the variable ... information on how much the mutant affects the activity of the protein Further information can be gathered by considering which parameters have the largest contribution to the total score If the most ... judge the importance of all parameters, regardless of their signs We see that the energy parameter is, on average, responsible for almost onethird of the information used in the prediction Conservation,...
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Tài liệu Báo cáo khoa học: Identification and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf
... genes from other species For example, the location of the intron within the MH1-encoding region is conserved in the human Smad4 gene, whereas the intron within the linker-encoding region of SmSmad1B ... revealed the interaction of SmSmad4 with SmGCN5 and demonstrated its participation in the formation and, probably, the stabilization of the transcriptional protein complex Figure 9B shows that the ... Analysis of the 5¢ upstream region of exon demonstrated the lack of a conserved TATA box upstream of the transcription initiation site However, an AT-rich sequence with a single nucleotide mismatch from...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... the -OH on C2, C3 and C4, which have the same configuration as those in galactose and lactose, are responsible for the strong interaction with the isoforms Interestingly, P II was the only isoform ... whole amount of the cDNA obtained in the reaction described above was used Several primer designs, based on the N-terminal amino acid sequence of the isoforms and on the DNA sequence of pulchellin ... wells containing 50 lL of NaCl ⁄ Pi Then, 50 lL of each isoform solution (112 lgÆmL)1) was added to the wells Following incubation, 50 lL of a 1% erythrocyte solution was added Numbers indicate the...
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Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in the pRN1 prim–pol structure ... designing the truncated Rep245 protein containing the residues predicted to be responsible for polymerase and primase catalysis, and reported on the functional characterization of the main functions of ... inclusion of the extraportion of the protein in Rep516 The Rep245 protein typifies the shortest functional domain among those endowed with primase and polymerase activities Based on the design of the...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... reaction was initiated by addition of the enzyme to the substrate solution, and the oxidation rate (nmolÆL)1Æs)1) was calculated from the linear part of the oxygen consumption curve The protein concentration ... (%) on monophenols and polyphenols from oxygen consumption (nmolÆL)1Æs)1) was calculated according to the stoichiometry that one monophenol molecule needs one oxygen molecule, and one polyphenol ... sequencing of purified tryptic peptides covered 39.1% of the sequence The molecular masses of the tryptic peptides, either from the mass fingerprint or purified peptides, covered 60.3% of the sequence, and...
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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... seven exons and six introns The sizes of all the exons except exon were found to be mostly conserved between PsCBL and AtCBL3 (Fig 2A and B) The PsCBL gene has an additional splice site at the fifth ... representation of the exon–intron organization of genomic PsCBL clone (A) and the Arabidopsis homologue (AtCBL3) clone (B) Closed boxes represent exons, and lines between closed boxes represent introns The ... sequencing and digestion with NcoI and EcoRI to give a band of 678 bp (Fig 6E, lane 2) On the other hand the complete ORF of PsCIPK (1.5 Kb) was cloned into the EcoRI and XhoI sites of yeast two-hybrid...
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