steroidogenic enzyme 17 20 lyase activity in cortisolsecreting and non functioning adrenocortica
Báo cáo khoa học: Interference with the citrulline-based nitric oxide synthase assay by argininosuccinate lyase activity in Arabidopsis extracts docx
... by ninhydrin staining, and then marked as dashed lines on the autoradiogram The origin was marked with an ink spot Argininosuccinate is the immediate precursor to arginine in the urea cycle, and ... and is converted to arginine and fumarate by argininosuccinate lyase (ASL; EC 4.3.2.1; Fig 5) Argininosuccinate is normally made from citrulline and aspartate by argininosuccinate synthetase, but ... assay Citrulline Hydroxyarginine Argininosuccinate Ornithine Origin 1-D 2-D Fig Two-dimensional TLC analysis of reaction product A reaction with [14C]arginine, desalted protein extract and ADF was...
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Báo cáo Y học: Baboon cytochrome P450 17a-hydroxylase/17,20-lyase (CYP17) Characterization of the adrenal microsomal and cloned enzymes docx
... activity of the enzyme, i.e substrate docking and binding, the active site including the heme-binding domain and redox partner binding domain [10] Baboon and human CYP17 are excellent candidates for ... adrenals and gonads [5] Mutations in the human gene result in the loss of a functional protein eliminating, in some clinical cases, only the 17, 20- lyase activity [6] or both the 17a-hydroxylase and 17, 20- lyase ... prediction of domains involved in substrate binding and redox partner interaction [10 ,17] Although site-directed mutageneses and naturally occurring CYP17 mutants have pinpointed specific amino acid residues...
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Báo cáo khoa học: Regulation of matrix metalloproteinase activity in health and disease pdf
... hemopexin-like domain (C domain) of human gelatinase A (matrix metalloproteinase-2) requires Ca2+ for fibronectin and heparin binding Binding properties of recombinant gelatinase A C domain to ... sialoprotein binds MMP-2, osteopontin binds MMP-3 and dentin matrix protein-1 binds MMP-9, all with a : stoichiometric ratio and binding constants in the nanomolar range [26] These SIBLINGs and MMPs ... illustrated in Fig 2B (step 1) Interactions with small integrin-binding ligand N-linked glycoprotein (SIBLING) Individual members of the SIBLING family are known to bind strongly to both pro- and active...
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Báo cáo khoa học: Acetyl-CoA:1-O-alkyl-sn-glycero-3-phosphocholine acetyltransferase (lyso-PAF AT) activity in cortical and medullary human renal tissue docx
... system of creatinine phosphate and creatinine phosphate kinase exerted the same inhibitory effect on both standard PAF and the lipid product of similar aggregatory activity Mild alkaline hydrolysis ... microsomal protein are shown in Fig Linearity for up to 20 of incubation and 0.2 mgÆmL)1 of cortex microsomal protein and for up to 20 and 0.12 mgÆmL)1 of medulla microsomal protein was observed ... effective than BSA in binding PAF or lyso-PAF [31] In our assays, it seems that the microsomal proteins can bind PAF effectively preventing it from inhibiting lyso-PAF AT activity and the addition...
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Báo cáo khoa học: Identification of a preferred substrate peptide for transglutaminase 3 and detection of in situ activity in skin and hair follicles pdf
... differentiation, TGase and TGase are believed to act cooperatively in the cross-linking of proteins, including involucrin, loricrin and small Preferred substrate peptide for TGase proline-rich proteins (SPRs) ... [23,24] Furthermore, TGase in hair follicles is involved in crosslinking structural proteins such as trichohyalin and keratin intermediate to hardening the inner root sheath In this case, TGase co-operates ... cross-linking structural proteins in keratinocytes [7–9] TGase is expressed in the prostate and is reported to be involved in plug formation in rodents [10] The biochemical characterization and...
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research report 'using the activity in pairs and in groups to teach writing in english'
... everyone is involved, but not staying too long with any group since the main initiative for writing should rest with students Group activity has two practical benefits in the teaching of writing Firstly, ... teaching writing to the first year students with an intermediate knowledge of English who study in Vinh university The writing games can be classified into group according to the teaching aims and ... command is given and the procedure is repeated - When the work has finished, the students open them and, in writing, join the fragments of information together to make it more interesting and logical...
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Báo cáo khoa hoc:" Genetic relationship between cyclic ovarian activity in heifers and cows and beef traits in males" ppt
... as (Renand, personal communication): IS = 0.426 IFW − 0.655 IRFI where IFW (final weight index) and IRFI (residual feed intake index) are standardised indexes obtained from: I = 100 + 20 ¯ g−g ... intake was daily recorded At the end of the test, breeding values were computed for final live weight and residual feed intake and a selection index (IS ) was used for ranking the bulls This index ... levels: 3–4; 5–6 and 7+ years) Cyclicity and beef traits: genetic relationship Twinningt an Final ageiktn eijktn 279 = fixed effect of type of birth t (2 levels: single or twin) = random additive...
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Báo cáo y học: "Vascular Endothelial Growth Factor (VEGF) isoform expression and activity in human and murine lung injury" pps
... Several lines of in vitro evidence have pointed to a possible role for VEGF in lung repair and recovery following injury[19,29,35,36] In one LPSinduced murine model of lung injury, intrapulmonary ... depicting 3ng/ml and combination confirming a significant and cell number (B) following treatment for 48 hours with Bar Bar graphs depicting 3H-thymidine incorporation into ATII cells (A) and cell ... length: VEGF121, VEGF165, VEGF189 being the main forms[3,1214] Exon (not present in VEGF121 and VEGF165) and exon provide heparin-binding affinity, exon (present in all active isoforms) is necessary...
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Regulation of sla2p by activity in endocytosis and actin organization by the ark1 prk1 family of kinases
... C-terminal domain ( 720- 968 amino acids) sows significant homology to filamentous actin 24 CHAPTER I INTRODUCTION binding protein Talin and has been shown to bind actin via its I/LWEQ actin-binding ... N-terminal of the protein acted in concert with WA to activate Arp2/3 complex In vivo, Las17p is under stringent regulation by a host of proteins (Rodal et al, 200 3) and is maintained in an inactive ... also contain EH domain-binding NPF (asparagine-prolinephenylalanine) motifs and a clathrin-binding motif (CBM) These proteins are found to localize to endocytic plasma membrane patches In mammalian...
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RECRUITMENT IN EXECUTIVE AND NON-EXECUTIVE CADRE pot
... Manufacturing Industries Three years Diploma in Engineering with 60% marks in aggregate from a recognised Indian University/Institute in the following respective branches Mechanical Engineering 04 ... Rs.7 lacs Rs 4200 Rs.6 lacs Rs 9300-34800 Rs 4200 Rs.5.5 lacs Rs 13640 Rs 5200 -202 00 Rs 2800 Rs.4.75 lacs PB-1 Rs 10160 Rs 5200 -202 00 Rs 200 0 Rs.3.75 lacs PB-1 Rs 9280 Rs 5200 -202 00 Rs 1900 Rs.3.50 ... examination, interview, selection and any other matter relating to recruitment will be final and binding on the candidate (22) The Candidates may please take note that the factory being a continuous...
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Comparision between background concentration of arsenic in urban and non urban areas of florida
... distribution in (a) Gainesville (ns200), (b) Miami (ns240), and non- urban areas (ns448) in Florida centrations in non- urban areas surrounding the two cities and land-use categories analyzed within the ... between urban and non- urban areas Therefore, knowing the distribution of arsenic in these three populations in both urban and non- urban soils aids our understanding of the impacts of human activity ... Edelman and de Bruin, 1986) suggesting that clay content is important in controlling the level and distribution of trace metal concentrations in soils A study conducted in both urban and non- urban...
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Báo cáo khoa học: Identification of proNeuropeptide FFA peptides processed in neuronal and non-neuronal cells and in nervous tissue potx
... N-terminal sequence of NPFF in mammals and recent reports demonstrating the importance of a protein convertase, the subtilisin/kexin isozyme SKI-1, in the processing of prohormones at specific nonbasic ... Characterization and inhibition of a cholecystokinin-inactivating serine peptidase Nature 380, 403–409 Eipper, B.A., Milgram, S.L., Husten, E.J., Yun, H.Y & Mains, R.E (1993) Peptidylglycine alpha-amidating ... most abundant in rat spinal cord [31] NPFF and NPSF were also identified in the mouse spinal cord [31], NPAF and NPSF in human cerebrospinal fluid [32] These reports suggested a processing of proNPFFA...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 3 pptx
... assumption of a linear decline in Pmax is sometimes incorporated into leaf lifetime carbon gain models (Hiremath, 200 0; Kikuzawa 1991) As it turns out, making an assumption of a linear decline in Pmax ... ~30% increase Anti-sense lowered expression of succinate dehydrogenase gene SDH2-2 Point mutation in sdd1-1 ~30% increase ~20% increase ~30% increase not reported Lower succinate dehydrogenase activity ... to capture inorganic carbon and concentrate it in chloroplasts near Calvin cycle machinery (Sage, 200 4) This high Cc sufficiently inhibits RuBP oxygenation reactions and virtually eliminates photorespiration...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 4 pot
... the intrinsic dissipation, we postulate an elastic domain defined by a convex function : ( , , )≤0 (79) The domain contains the origin, in other words: (0,0,0) < (80) Introducing the classic standard ... (108) Specialising to a linear isotropic porous material, after substituting (107) into (76) and taking into consideration the decomposition into the mean and deviatoric parts, and the initial stresses ... Following Coussy (200 4), we split the strain and porosity variation into a elastic and a plastic part: = + ; = + ; Δ = − = + (60) We denote by and the volumetric component of the skeleton strain and...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 5 doc
... follows: Heating with linear increasing of the temperature in a wide range from 20 0С to 200 0С with heating rate of 3.5 0С/min under constant strain stress from МРа to 1.7 МРа (increasing step of ... 0.12 MPa at a linear heating with the same heating rate (3.5 0C/min) and again in the temperature range from 20 0C to 200 0C The structural tests of the SHMM samples in this preliminary experiment ... premelting and melting endotherms in Fig smoothly shifts to higher temperatures with stress increasing Multiple melting peaks in PET pellets (Kong & Hay, 200 3) and filaments are observed and studied...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 6 doc
... interesting in that it works by supplying heat energy regardless of its origin (waste heat, geothermal water, solar energy ) Operating principle The operating principle of such a machine is briefly ... Systems in Equilibrium and Non- Equilibrium Conclusion In this investigation, single and double-stage absorption cycles using water-ammonia are analyzed We presented different configurations and we ... Refrigerating System Operating at Low Enthalpy Sources Nahla Bouaziz, Ridha BenIffa, Ezzedine Nehdi and Lakdar Kairouani Engineering National School of Tunis Tunisia Introduction Since the work...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 7 doc
... landscape 152 Thermodynamics – Systems in Equilibrium and Non- Equilibrium 4.3 Main transformation modalities in the landscape In a landscape or in its subsystems (i.e Landscape Units) the main ... capacity or BTC (Ingegnoli 1991, 200 2; Ingegnoli and Giglio 1999, 200 5, Ingegnoli and Pignatti, 200 7), which represents the flux of energy that an ecocoenotope must dissipate to maintain its proper ... transforming itself following the time arrow, in a non- finalistic evolution and in a creative way That is why history has becoming indispensable Without it, it is simply impossible to understand properly...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 8 doc
... (lines are eye-guides), and b) corresponding H/T versus 1/T plot (lines are linear fits to isomagnetic points) 182 Thermodynamics – Systems in Equilibrium and Non- Equilibrium Will-be-set-by -IN- TECH ... construct the scaling plot, using the obtained λ1 and λ3 parameters (Fig 8(b)) From the scaling plot and the subsequent fit with the Brillouin function, we obtain values of spin and saturation magnetization ... from the non- equilibrium thermodynamics, concerning living systems and, consequently, (b) to revise the main concepts of vegetation science in the light of the new “Landscape Bionomics” and indicate...
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Thermodynamics Systems in Equilibrium and Non Equilibrium Part 9 doc
... and Q are respectively the Prandtl number and the heat/sink k k parameter Introducing the variable Pr 2 e (17) And inserting (17) in (15) we obtain Pr Pr 1 ... FEDER-COMPETE and FCT through Projects PTDC/CTM-NAN/115125 /200 9, PTDC/FIS/105416 /200 8, CERN/FP/116 320/ 2010, grants SFRH/BPD/39262 /200 7 (S Das) and SFRH/BPD/63942 /200 9 (J S Amaral) References Aharoni, A (200 0) ... i.e., the system is in thermodynamic equilibrium and is homogeneous Numerically integrating the Maxwell relation corresponds to integrating the magnetic isotherms in field, and dividing by the temperature...
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