stem progenitor cells used as vehicles for gene therapy for lung diseases
Báo cáo y học: "Differential expression, function and response to inflammatory stimuli of 11β-hydroxysteroid dehydrogenase type 1 in human fibroblasts: a mechanism for tissue-specific regulation of inflammation" pps
... target gene - Ct of housekeeping gene) as raw data for gene expression (high ∆Ct = low gene expression) The fold change in gene expression was determined by subtracting ∆Ct values for treated cells ... (10 ng/ml) for 24 hours Total RNA from these cells was then used to assess expression of mRNA for 11βHSD1 In each case mRNA levels for 11β-HSD1 were normalized for the housekeeping gene 18S rRNA ... µmol/l) for 24 hours Cells were then assessed expression of IL-6 mRNA and protein (a) For mRNA analyses, target gene data were normalized for levels of the housekeeping gene 18S rRNA and presented as...
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Báo cáo khoa học: "A Construction-Specific Approach to Focused Interaction in Flexible Parsing" ppt
... after", for instance, is equivalent to "since" for this purpose In the example, the Syntax slot defines the input syntax for a message; it is used to generate rules for RexP, which ere in turn used ... interactive system is impossible In such circumstances, the parser should interact with the system user to resolve the problem Moreover, to make things as easy as possible for the user, the system should ... then generating patterns for each of the Cases, substituting the appropriate FillerTypes for the slot names that appear in the patterns used to define the Cases, thus generating the subpatterns:...
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báo cáo hóa học: " Comparison of the discriminative ability of a generic and a condition-specific OHRQoL measure in adolescents with and without normative need for orthodontic treatment" potx
... effect size for OHIP-14 was nil when DAI was used to define normative need for orthodontic treatment When the prevalence of oral impacts, calculated by each OHRQoL measure, was used to assess the ... orthodontic treatment This was the first attempt to assess the discriminative ability of both OHRQoL measures When overall scores for both OHRQoL measures were used to assess the impacts of oral ... be provided for individuals who have a DHC grade of or 5, or grade with an AC of or above All other cases were therefore classified as having no need For DAI, 10 occlusal traits were assessed and...
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báo cáo hóa học: " Comparing a disease-specific and a generic health-related quality of life instrument in subjects with asthma from the general population" pot
... Initiative for Asthma: [http://www.ginasthma.com/] Bonii G, Rezzani C, Grassi M, Marinoni A: ECRHS screening questionnaire scoring : a methodological suggestion for asthma assessment J Outcome Meas 2001, ... 50 for the general population with a standard deviation of 10 Validation measures We used a number of validation measures available from the SAPALDIA database that met the Global Initiative for ... body mass index; GP: general practitioner; FVC: forced vital capacity; FEV1: forced expiratory volume at one second; FEF25–75: forced expiratory flow measured between 25% and 75% of the forced...
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Báo cáo hóa học: " Experimental observations of rapid Maize streak virus evolution reveal a strand-specific nucleotide substitution bias" potx
... whereas our estimates are based on mutational distances from known progenitor sequences, theirs are based on distances from a population consensus sequence Had we used a consensus of the 17 MSV-Tas ... That an increased rate of G→T transversions has been associated with time spent as ssDNA [65-67] fits very well with the notion that increased rates of MSV mutation may be at least partially ... -Tas infections In both the MSV-Kom/Set and the MSV-Tas datasets, neither the mp-cp cassette nor the repA-repB cassette contained disproportionately more mutations than could be accounted for...
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báo cáo hóa học:" Application of a disease-specific mapping function to estimate utility gains with effective treatment of schizophrenia" pot
... colleagues, has been to develop a health index based on the content of a short-form measure and to measure utilities for the models with larger numbers of states as defined by the shortform measure ... responsiveness of the measures, we took the standard deviation of SF-36-based and clinically based utility measures at baseline and endpoint and the effect size seen in this study for each measure and estimated ... other mapping functions for short-form measures in depression [14] Use of a health index model or a short-form measure of health status does not address the issue of disease-specific effects on...
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Báo cáo khoa học: " Effects of L-NAME, a non-specific nitric oxide synthase inhibitor, on AlCl3-induced toxicity in the rat forebrain cortex" doc
... spectrophotometric assay for superoxide dismutase based on epinephrine autoxidation Anal Biochem 1978, 90, 81-89 35 Tanino H, Shimohama S, Sasaki Y, Sumida Y, Fujimoto S Increase in phospholipase C-delta1 ... responsible, at least in part, for some of the neurodegenerative changes caused by stress and support a possible neuroprotective role for NOS inhibitors in this context [26] Decreased bilateral ... was derived from polysaturated fatty acids during peroxidation The reaction product, MDA, was measured spectrophotometrically at 533 nm [39] SOD activity was measured spectrophotometrically as...
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Báo cáo khoa học: "Myxoid liposarcoma: a rare soft-tissue tumor with a misleading benign appearance" docx
... the other extremity of the spectrum of disease, the LPS featuring a majority of round cells, metastasizes in 70% of the cases These metastases arise in lungs and bone but, also, in serous membranes ... years later [10] Some genetic diseases (neuromatosis, retinoblastoma, L-Fraumeni syndrome) may lead to the development of soft-tissue sarcomas Lipoma is a very common tumor, generally, of a small ... external radiation therapy and genetic factors are the most well-established risk factors for soft tissue sarcomas Approximately 1% of patients treated with radiation therapy for a malignant tumor,...
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Báo cáo y học: "Long-term cyclical in vivo loading increases cartilage proteoglycan content in a spatially specific manner: an infrared microspectroscopic imaging and polarized light microscopy study" ppt
... proteoglycan was measured as the mean integrated area of the sugar peak (1,185 - 960 cm-1) in the ROI defined above for each FTIR image [18] The amount of collagen was measured as the mean integrated ... Yuka Nakamura for animal handling, Alex Portnoy for tissue sectioning, and Andrew Burghardt for assistance with the FTIR techniques This work was supported by the Centers for Disease Control and ... transilluminated by polarized light A × 20 objective was used for digital image collection Axiovision software version 3.1 (Carl Zeiss) was used for camera control and digital image collection During...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... epitheloid cells rapidly disappeared from the culture as early as passage At later passages, T-MPCs were homogeneously fibroblast-like, with extended cytoplasmic processes Morphologically, these cells ... adipogenesis genes: lipoprotein lipase (LPL) and peroxisome proliferator-activated receptor-gamma (PPARγ), (b) osteogenesis genes: alkaline phosphatase (ALP) and osteocalcin (OC), and (c) chondrogenesis ... medium for weeks, fixed with 10% formalin, and stained with Giemsa (SigmaAldrich), and colonies of fibroblast-like cells were identified and counted based on the methods described by CastroMalaspina...
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Báo cáo y học: "Development and validation of the self-administered Fibromyalgia Assessment Status: a disease-specific composite measure for evaluating treatment effect" pptx
... were more often classified as having high levels of disease activity, and stratification into three categories confirmed that increasing education was associated with lower FAS values: primary ... whereas respondents with a low educational level were more often classified as having a high degree of disease activity It has been reported that years of formal education are a risk factor for ... distributional assumptions The chosen level of significance was α = 0.05 ES is calculated as the mean change in score from baseline divided by the standard deviation of the baseline scores, whereas SRM...
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Báo cáo y học: " Mitogen-activated protein kinases and NFκB are involved in SP-A-enhanced responses of macrophages to " pdf
... increased kinase activity, in vitro kinase assays were performed Cells were treated with BCG or SPA-BCG for and 15 Control cells were incubated for 15 with SP-A alone Total cellular protein was ... follows: cells were incubated with BCG or SPA-BCG for hr at 37°C The cells were washed, and DMEM containing 10% serum plus 2.5 μCi of 3H-uracil was added to each well Assays were performed in ... using one-way ANOVA In all cases, a p value of < 0.05 was considered significant Data in figures are expressed as mean ± SD Results Immunoprecipitation and kinase assays Cells were incubated with...
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Báo cáo y học: "The SLC2A9 non-synonymous Arg265His variant and gout; evidence for a population-specific effect on severit" docx
... equilibrium P values > 0.003 for all case and control sample sets STRUCTURE [24] was used to assign Māori, East Polynesian and West Polynesian individuals into non-Caucasian populations (parameters; ... heterogeneity in genetic association with gout in the Asia-Pacific region has also been documented at ABCG2 [19] Furthermore, given that the C-allele frequency was similar between Māori and Caucasian ... [12,13] Of the other variants, only P321L has been tested for association with gout in an Asian- 12 Pacific population – there was no evidence for association in Japanese [13] The impact of these...
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Báo cáo y học: " Tuberculosis contact investigation with a new, specific blood test in a low-incidence population containing a high proportion of BCG-vaccinated persons" pdf
... incubated at 37°C for1 6– 24 hours, after which they were centrifuged and plasma harvested Plasma was kept refrigerated at 4–6°C until the ELISA was performed The IFN-γ ELISA was performed using the ... with live vaccines As there was no evidence of suspicion of immunosuppression for any of the contacts, an optional PHA mitogen control tube for the QFT-G test was not performed for this study The ... equivalent to about TU of PPD-S) was injected intradermally into the volar aspect of the forearm and transverse induration diameter was measured 72 hours later Individuals performing the TST were blinded...
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Báo cáo sinh học: "Syntenator: Multiple gene order alignments with a gene-specific scoring function" docx
... release 46: 1) Ensembl orthologs (1:1, 1:many and many:many), 2) Best reciprocal BLASTP hits (BRH) and 3) 3-best-reciprocal BLASTP hits (BRH3) In the last case, a gene may have up to hits Generally, ... similarity The genomic context of a gene copy might provide additional information as to the gene' s evolutionary history Gene copies that are surrounded by the same genes in different genomes are ... a gene- gene mapping or mapping of vertices of the two input graphs (Figure 1C) Three possible scenarios may occur in this simple example: two genes match, two genes not match or one gene has...
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Báo cáo y học: "Latency profiles of full length HIV-1 molecular clone variants with a subtype specific promoter" pps
... Performance of the HIV-1 latency assay A: Average percentages of CA-p24 positive cells as determined by FACS in SupT1 T cells infected with increasing concentrations of HIV-1LAI (ng/infection) Cells ... linear range of the assay, SupT1 cells were infected with different amounts of subtype AE or B virus As expected, the basal percentage of CA-p24 positive cells was always higher for AE than B (Figure ... Flow cytometry was performed with RD1- or FITC-conjugated mouse monoclonal anti-CA-p24 (clone KC57, Coulter) Cells were fixed in 4% formaldehyde for at least at room temperature, washed with FACS...
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Báo cáo y học: "Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line" ppsx
... showing CD3+ lymphoma cells in lung metastases (IHC, hematoxylin, ×200) zone B cell lymphoma (MZL) as well as early thymic T-LL To our knowledge, spontaneous splenic MZL has not been reported ... Thus, Fv1n cells are permissive for N-tropic and partially restrictive for Btropic MuLVs; the reciprocal pattern is observed for cells of Fv1b mice A few isolates with long laboratory passage histories, ... proposals for classification of lymphoid neoplasms in mice Blood 2002, 100:246-258 Vasmel WLE, Zijlstra M, Radaszkiewicz T, Leupers CJM, de Goede REY, Melief CJM: Major histocompatibility complex class...
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A site specific design of a fixed pitch fixed speed wind turbine blade with multiple airfoils as design variable
... y-coordinates max 0.04 0.15 Same as Pt 0.08 0.2 Same as Pt 0.02 0.10 -0.10 -0.01 Same as Pt 10 -0.20 -0.08 Same as Pt 10 -0.10 0.07 The problem yields six variables for the chord, six for the twist, and each ... relative step of 0.15 for the finite difference step was implemented on the AEP calculation for an optimization of the design The starting point for the search was the design of the Phase VI wind turbine ... relative thickness As it was previously established by Grasso [12] a variation in relative thickness is recommended for the inner part of the blade to be larger than the outer part for structural...
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Glial cell line drived neurotrophic factor (GDNF) family of ligands is a mitogenic agent in human glioblastoma and confers chemoresistance in a ligand specific fashion
... oligodendrogliomas are some of the examples 1.2 Astrocytomas The term astrocytoma was used as early as the late 19th century by Virchow2 but was only firmly used in histopathological classification ... as high-grade or malignant astrocytomas The most common WHO Grade III astrocytoma is anaplastic astrocytoma and the most common WHO Grade IV astrocytoma is glioblastoma High-grade astrocytomas ... microvascular proliferation and necrosis Similar to anaplastic astrocytoma, glioblastoma may arise de novo as glioblastoma or may transform from diffuse astrocytoma (WHO Grade II) or anaplastic astrocytoma...
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Identification and characterization of IFI30 as a glioblastoma specific promoter for glioma gene therapy
... gene therapy As gene therapy for GBM is localised there is minimum risk of systemic toxicity (Pulkkanen and Yla-Herttuala, 2005) Gene therapy is the transfer of exogenous genes, called transgenes, ... somatic cells of a patient to obtain a therapeutic effect Initially gene therapy was considered as an approach for treating hereditary diseases, but it's potential in the treatment of acquired diseases ... Baculovirus as a vector for gene therapy There is an increasing use of the insect baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV)-based vectors as gene delivery vectors for...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25